################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvaI AvrII BanI BbeI BbrPI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoNI FbaI FseI HaeII Hin1I HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1000 GTCGAC GT^[AC][GT]AC AgeI 223 ACCGGT A^CCGGT AhdI 1125 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 2254 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1189 CCTGAGG CC^T[ACGT]AGG ApaI 1352 GGGCCC GGGCC^C AspEI 1125 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3116 TGCGCA TGC^GCA BcgI 371 CGAATGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 3053 AGATCT A^GATCT BpmI 1292 CTGGAG CTGGAG(16/14) BsaBI 3535 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 2653 CTCCTC GAGGAG(10/8) Bsp1407I 2780 TGTACA T^GTACA BspMI 3113 ACCTGC ACCTGC(4/8) BsrBI 1084 CCGCTC CCGCTC(-3/-3) BsrFI 223 ACCGGT [GA]^CCGG[CT] BsrGI 2780 TGTACA T^GTACA BstAPI 3166 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1620 GGTAACC G^GT[ACGT]ACC BstPI 1620 GGTAACC G^GT[ACGT]ACC Bsu36I 1189 CCTGAGG CC^T[ACGT]AGG CfoI 3117 GCGC GCG^C Cfr10I 223 ACCGGT [GA]^CCGG[CT] DraIII 97 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1475 GACAACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1125 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1189 CCTGAGG CC^T[ACGT]AGG EcoO65I 1620 GGTAACC G^GT[ACGT]ACC EcoRI 2947 GAATTC G^AATTC EcoRV 1642 GATATC GAT^ATC FauI 1083 CCCGC CCCGC(4/6) FspI 3116 TGCGCA TGC^GCA HgaI 207 GACGC GACGC(5/10) HhaI 3117 GCGC GCG^C HinP1I 3117 GCGC G^CGC HincII 1000 GTCGAC GT[CT]^[GA]AC HindII 1000 GTCGAC GT[CT]^[GA]AC MamI 3535 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 2570 CATATG CA^TATG PinAI 223 ACCGGT A^CCGGT SacI 535 GAGCTC GAGCT^C SalI 1000 GTCGAC G^TCGAC SapI 2141 GAAGAGC GCTCTTC(1/4) SexAI 500 ACCAGGT A^CC[AT]GGT SfcI 1149 CTATAG C^T[GA][CT]AG SspBI 2780 TGTACA T^GTACA StuI 348 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflII 456 CTTAAG C^TTAAG 1830 CTTAAG BamHI 2067 GGATCC G^GATCC 2265 GGATCC BbvCI 972 CCTCAGC CCTCAGC(-5/-2) 1721 CCTCAGC BfrI 456 CTTAAG C^TTAAG 1830 CTTAAG BsaWI 223 ACCGGT [AT]^CCGG[AT] 3083 ACCGGA BsmI 951 GAATGC GAATGC(1/-1) 3166 GCATTC BspLU11I 1926 ACATGT A^CATGT 2777 ACATGT DsaI 1496 CCATGG C^C[GA][CT]GG 3500 CCATGG HapII 224 CCGG C^CGG 3084 CCGG HindIII 744 AAGCTT A^AGCTT 1871 AAGCTT HpaII 224 CCGG C^CGG 3084 CCGG MaeII 1547 ACGT A^CGT 2371 ACGT MspA1I 975 CAGCTG C[AC]G^C[GT]G 1107 CAGCTG MspI 224 CCGG C^CGG 3084 CCGG NcoI 1496 CCATGG C^CATGG 3500 CCATGG PvuII 975 CAGCTG CAG^CTG 1107 CAGCTG XcmI 116 CCAACTGCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3029 CCATGTTCATTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BalI BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MunI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI