(Linear) (Six-base) MAPSORT of: KIEE9425.seq Check: 190 from: 1 to: 3666 KIEE9425.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AccI GT'mk_AC Cuts at: 0 1001 3666 Size: 1001 2665 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1889 2281 3630 3666 Size: 1889 392 1349 36 Fragments arranged by size: 1889 1349 392 36 AflII C'TTAA_G Cuts at: 0 456 1830 3666 Size: 456 1374 1836 Fragments arranged by size: 1836 1374 456 AflIII A'CryG_T Cuts at: 0 1926 2369 2777 3666 Size: 1926 443 408 889 Fragments arranged by size: 1926 889 443 408 AgeI A'CCGG_T Cuts at: 0 223 3666 Size: 223 3443 AhdI GACnn_n'nnGTC Cuts at: 0 1130 3666 Size: 1130 2536 AleI CACnn'nnGTG Cuts at: 0 1286 3666 Size: 1286 2380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2653 3666 Size: 2653 1013 AlwNI CAG_nnn'CTG Cuts at: 0 2259 3666 Size: 2259 1407 ApaI G_GGCC'C Cuts at: 0 1356 3666 Size: 1356 2310 ApoI r'AATT_y Cuts at: 0 325 798 939 1692 1998 2109 2833 2947 Size: 325 473 141 753 306 111 724 114 Cuts at: 2947 3666 Size: 719 Fragments arranged by size: 753 724 719 473 325 306 141 114 111 BamHI G'GATC_C Cuts at: 0 2067 2265 3666 Size: 2067 198 1401 Fragments arranged by size: 2067 1401 198 BanII G_rGCy'C Cuts at: 0 219 539 787 883 1356 3666 Size: 219 320 248 96 473 2310 Fragments arranged by size: 2310 473 320 248 219 96 BbsI GAAGACnn'nnnn_ Cuts at: 0 590 734 2005 3392 3666 Size: 590 144 1271 1387 274 Fragments arranged by size: 1387 1271 590 274 144 BbvCI CC'TCA_GC Cuts at: 0 973 1722 3666 Size: 973 749 1944 Fragments arranged by size: 1944 973 749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 360 394 3666 Size: 360 34 3272 Fragments arranged by size: 3272 360 34 BfrBI ATG'CAT Cuts at: 0 381 619 706 3666 Size: 381 238 87 2960 Fragments arranged by size: 2960 381 238 87 BglII A'GATC_T Cuts at: 0 3053 3666 Size: 3053 613 Bme1580I G_kGCm'C Cuts at: 0 1356 3666 Size: 1356 2310 BmgBI CAC'GTC Cuts at: 0 2372 3666 Size: 2372 1294 BmrI ACTGGGnnnn_n' Cuts at: 0 44 1489 1638 3666 Size: 44 1445 149 2028 Fragments arranged by size: 2028 1445 149 44 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1313 3666 Size: 1313 2353 Bpu10I CC'TnA_GC Cuts at: 0 973 1060 1722 2626 3666 Size: 973 87 662 904 1040 Fragments arranged by size: 1040 973 904 662 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 348 1345 1470 1876 2125 3666 Size: 348 997 125 406 249 1541 Fragments arranged by size: 1541 997 406 348 249 125 BsaBI GATnn'nnATC Cuts at: 0 3539 3666 Size: 3539 127 BsaWI w'CCGG_w Cuts at: 0 223 3083 3666 Size: 223 2860 583 Fragments arranged by size: 2860 583 223 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 3196 3226 3666 Size: 3196 30 440 Fragments arranged by size: 3196 440 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2644 3666 Size: 2644 1022 BseYI C'CCAG_C Cuts at: 0 1089 2849 2992 3666 Size: 1089 1760 143 674 Fragments arranged by size: 1760 1089 674 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1133 2250 3070 3666 Size: 1133 1117 820 596 Fragments arranged by size: 1133 1117 820 596 BsiHKAI G_wGCw'C Cuts at: 0 539 2148 2632 3666 Size: 539 1609 484 1034 Fragments arranged by size: 1609 1034 539 484 BsmI GAATG_Cn' Cuts at: 0 957 3166 3666 Size: 957 2209 500 Fragments arranged by size: 2209 957 500 Bsp1286I G_dGCh'C Cuts at: 0 219 539 787 883 1356 2148 2632 3666 Size: 219 320 248 96 473 792 484 1034 Fragments arranged by size: 1034 792 484 473 320 248 219 96 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3122 3666 Size: 3122 544 BsrBI CCG'CTC Cuts at: 0 1086 3666 Size: 1086 2580 BsrDI GCAATG_nn' Cuts at: 0 2705 2783 3023 3603 3666 Size: 2705 78 240 580 63 Fragments arranged by size: 2705 580 240 78 63 BsrFI r'CCGG_y Cuts at: 0 223 3666 Size: 223 3443 BsrGI T'GTAC_A Cuts at: 0 2780 3666 Size: 2780 886 BstAPI GCAn_nnn'nTGC Cuts at: 0 3172 3666 Size: 3172 494 BstEII G'GTnAC_C Cuts at: 0 1620 3666 Size: 1620 2046 BstXI CCAn_nnnn'nTGG Cuts at: 0 1617 1743 3535 3666 Size: 1617 126 1792 131 Fragments arranged by size: 1792 1617 131 126 BstYI r'GATC_y Cuts at: 0 2067 2265 2334 2964 3053 3666 Size: 2067 198 69 630 89 613 Fragments arranged by size: 2067 630 613 198 89 69 Bsu36I CC'TnA_GG Cuts at: 0 1190 3666 Size: 1190 2476 BtgI C'CryG_G Cuts at: 0 1496 3500 3666 Size: 1496 2004 166 Fragments arranged by size: 2004 1496 166 BtsI GCAGTG_nn' Cuts at: 0 3126 3666 Size: 3126 540 DraI TTT'AAA Cuts at: 0 1658 1691 1877 3666 Size: 1658 33 186 1789 Fragments arranged by size: 1789 1658 186 33 DraIII CAC_nnn'GTG Cuts at: 0 102 3666 Size: 102 3564 DrdI GACnn_nn'nnGTC Cuts at: 0 1481 3666 Size: 1481 2185 EaeI y'GGCC_r Cuts at: 0 576 1103 1522 3666 Size: 576 527 419 2144 Fragments arranged by size: 2144 576 527 419 EarI CTCTTCn'nnn_ Cuts at: 0 2136 2662 3214 3666 Size: 2136 526 552 452 Fragments arranged by size: 2136 552 526 452 EcoICRI GAG'CTC Cuts at: 0 537 3666 Size: 537 3129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1313 1889 2281 3630 3666 Size: 1313 576 392 1349 36 Fragments arranged by size: 1349 1313 576 392 36 EcoO109I rG'GnC_Cy Cuts at: 0 149 1124 1353 1633 2191 3181 3666 Size: 149 975 229 280 558 990 485 Fragments arranged by size: 990 975 558 485 280 229 149 EcoRI G'AATT_C Cuts at: 0 2947 3666 Size: 2947 719 EcoRV GAT'ATC Cuts at: 0 1644 3666 Size: 1644 2022 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1590 2222 3666 Size: 1590 632 1444 Fragments arranged by size: 1590 1444 632 FspI TGC'GCA Cuts at: 0 3118 3666 Size: 3118 548 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 280 312 1116 1148 2590 2622 2949 2981 Size: 280 32 804 32 1442 32 327 32 Cuts at: 2981 3532 3564 3666 Size: 551 32 102 Fragments arranged by size: 1442 804 551 327 280 102 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1002 3666 Size: 1002 2664 HindIII A'AGCT_T Cuts at: 0 744 1871 3666 Size: 744 1127 1795 Fragments arranged by size: 1795 1127 744 MfeI C'AATT_G Cuts at: 0 276 631 1883 3666 Size: 276 355 1252 1783 Fragments arranged by size: 1783 1252 355 276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 161 992 1557 1673 2935 3443 3666 Size: 161 831 565 116 1262 508 223 Fragments arranged by size: 1262 831 565 508 223 161 116 MscI TGG'CCA Cuts at: 0 578 1105 1524 3666 Size: 578 527 419 2142 Fragments arranged by size: 2142 578 527 419 MslI CAynn'nnrTG Cuts at: 0 1286 3028 3533 3666 Size: 1286 1742 505 133 Fragments arranged by size: 1742 1286 505 133 MspA1I CmG'CkG Cuts at: 0 977 1109 3666 Size: 977 132 2557 Fragments arranged by size: 2557 977 132 NcoI C'CATG_G Cuts at: 0 1496 3500 3666 Size: 1496 2004 166 Fragments arranged by size: 2004 1496 166 NdeI CA'TA_TG Cuts at: 0 2571 3666 Size: 2571 1095 NsiI A_TGCA'T Cuts at: 0 383 621 708 3666 Size: 383 238 87 2958 Fragments arranged by size: 2958 383 238 87 NspI r_CATG'y Cuts at: 0 204 385 1930 2781 3666 Size: 204 181 1545 851 885 Fragments arranged by size: 1545 885 851 204 181 PciI A'CATG_T Cuts at: 0 1926 2777 3666 Size: 1926 851 889 Fragments arranged by size: 1926 889 851 PfoI T'CCnGG_A Cuts at: 0 2180 3666 Size: 2180 1486 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1705 1737 3666 Size: 1705 32 1929 Fragments arranged by size: 1929 1705 32 PpuMI rG'GwC_Cy Cuts at: 0 149 1124 1633 2191 3181 3666 Size: 149 975 509 558 990 485 Fragments arranged by size: 990 975 558 509 485 149 PsiI TTA'TAA Cuts at: 0 923 3666 Size: 923 2743 PspOMI G'GGCC_C Cuts at: 0 1352 3666 Size: 1352 2314 PvuII CAG'CTG Cuts at: 0 977 1109 3666 Size: 977 132 2557 Fragments arranged by size: 2557 977 132 SacI G_AGCT'C Cuts at: 0 539 3666 Size: 539 3127 SalI G'TCGA_C Cuts at: 0 1000 3666 Size: 1000 2666 SanDI GG'GwC_CC Cuts at: 0 1124 3181 3666 Size: 1124 2057 485 Fragments arranged by size: 2057 1124 485 SapI GCTCTTCn'nnn_ Cuts at: 0 2136 3666 Size: 2136 1530 SexAI A'CCwGG_T Cuts at: 0 500 3666 Size: 500 3166 SfcI C'TryA_G Cuts at: 0 1149 3666 Size: 1149 2517 SmlI C'TyrA_G Cuts at: 0 363 456 1324 1449 1830 1855 2104 3666 Size: 363 93 868 125 381 25 249 1562 Fragments arranged by size: 1562 868 381 363 249 125 93 25 SspI AAT'ATT Cuts at: 0 1731 1935 3627 3666 Size: 1731 204 1692 39 Fragments arranged by size: 1731 1692 204 39 StuI AGG'CCT Cuts at: 0 350 3666 Size: 350 3316 StyI C'CwwG_G Cuts at: 0 1336 1436 1496 3500 3666 Size: 1336 100 60 2004 166 Fragments arranged by size: 2004 1336 166 100 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2516 3666 Size: 2516 1150 TatI w'GTAC_w Cuts at: 0 1200 2780 3666 Size: 1200 1580 886 Fragments arranged by size: 1580 1200 886 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 123 3036 3666 Size: 123 2913 630 Fragments arranged by size: 2913 630 123 XmnI GAAnn'nnTTC Cuts at: 0 421 821 1547 2771 3666 Size: 421 400 726 1224 895 Fragments arranged by size: 1224 895 726 421 400 Enzymes that do cut: AccI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApoI BamHI BanII BbsI BbvCI BcgI BfrBI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PciI PfoI PpiI PpuMI PsiI PspOMI PvuII SacI SalI SanDI SapI SexAI SfcI SmlI SspI StuI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BciVI BclI BglI BlpI BmtI BplI BsaI BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI EagI EciI EcoNI FseI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PmlI PshAI PspXI PsrI PstI PvuI RsrII SacII SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI XmaI ZraI