(Linear) (Six-base) MAPSORT of: KIEE4219.seq Check: 6541 from: 1 to: 2154 KIEE4219.orf With 182 enzymes: SgfI * July 3, 2012 18:47 .. AccI GT'mk_AC Cuts at: 0 98 2154 Size: 98 2056 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1501 1764 2154 Size: 1501 263 390 Fragments arranged by size: 1501 390 263 AhdI GACnn_n'nnGTC Cuts at: 0 504 2154 Size: 504 1650 AleI CACnn'nnGTG Cuts at: 0 451 2154 Size: 451 1703 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 223 2154 Size: 223 1931 AlwNI CAG_nnn'CTG Cuts at: 0 27 294 2154 Size: 27 267 1860 Fragments arranged by size: 1860 267 27 ApaI G_GGCC'C Cuts at: 0 109 136 2154 Size: 109 27 2018 Fragments arranged by size: 2018 109 27 ApoI r'AATT_y Cuts at: 0 1462 2154 Size: 1462 692 AvaI C'yCGr_G Cuts at: 0 236 259 2154 Size: 236 23 1895 Fragments arranged by size: 1895 236 23 BanI G'GyrC_C Cuts at: 0 363 369 400 2154 Size: 363 6 31 1754 Fragments arranged by size: 1754 363 31 6 BanII G_rGCy'C Cuts at: 0 109 136 636 950 1454 1877 2067 2154 Size: 109 27 500 314 504 423 190 87 Fragments arranged by size: 504 500 423 314 190 109 87 27 BbeI G_GCGC'C Cuts at: 0 367 373 2154 Size: 367 6 1781 Fragments arranged by size: 1781 367 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 1847 2154 Size: 1847 307 BciVI GTATCCnnnnn_n' Cuts at: 0 1946 2154 Size: 1946 208 BclI T'GATC_A Cuts at: 0 1212 2154 Size: 1212 942 BfrBI ATG'CAT Cuts at: 0 1634 2154 Size: 1634 520 BglI GCCn_nnn'nGGC Cuts at: 0 433 640 698 2154 Size: 433 207 58 1456 Fragments arranged by size: 1456 433 207 58 BlpI GC'TnA_GC Cuts at: 0 28 302 685 1871 2066 2154 Size: 28 274 383 1186 195 88 Fragments arranged by size: 1186 383 274 195 88 28 Bme1580I G_kGCm'C Cuts at: 0 109 136 2154 Size: 109 27 2018 Fragments arranged by size: 2018 109 27 BmrI ACTGGGnnnn_n' Cuts at: 0 626 2154 Size: 626 1528 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2086 2154 Size: 2086 68 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 964 2154 Size: 331 633 1190 Fragments arranged by size: 1190 633 331 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 775 2154 Size: 775 1379 BsaI GGTCTCn'nnnn_ Cuts at: 0 854 2154 Size: 854 1300 BsaHI Gr'CG_yC Cuts at: 0 224 232 364 370 1988 2154 Size: 224 8 132 6 1618 166 Fragments arranged by size: 1618 224 166 132 8 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1060 1090 1664 1694 2154 Size: 1060 30 574 30 460 Fragments arranged by size: 1060 574 460 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 685 1081 1084 1462 2154 Size: 685 396 3 378 692 Fragments arranged by size: 692 685 396 378 3 BseYI C'CCAG_C Cuts at: 0 643 717 1011 2016 2154 Size: 643 74 294 1005 138 Fragments arranged by size: 1005 643 294 138 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1576 2154 Size: 1576 578 BsiEI CG_ry'CG Cuts at: 0 567 2154 Size: 567 1587 BsiHKAI G_wGCw'C Cuts at: 0 686 950 1454 1790 1877 2067 2154 Size: 686 264 504 336 87 190 87 Fragments arranged by size: 686 504 336 264 190 87 87 BsmI GAATG_Cn' Cuts at: 0 888 1279 2154 Size: 888 391 875 Fragments arranged by size: 888 875 391 BsmBI CGTCTCn'nnnn_ Cuts at: 0 412 2154 Size: 412 1742 Bsp1286I G_dGCh'C Cuts at: 0 109 136 636 686 950 1454 1790 1877 Size: 109 27 500 50 264 504 336 87 Cuts at: 1877 2067 2154 Size: 190 87 Fragments arranged by size: 504 500 336 264 190 109 87 87 50 27 BspHI T'CATG_A Cuts at: 0 876 2154 Size: 876 1278 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1236 2154 Size: 1236 918 BsrBI CCG'CTC Cuts at: 0 285 1748 2154 Size: 285 1463 406 Fragments arranged by size: 1463 406 285 BsrDI GCAATG_nn' Cuts at: 0 1143 2090 2154 Size: 1143 947 64 Fragments arranged by size: 1143 947 64 BsrFI r'CCGG_y Cuts at: 0 366 566 2154 Size: 366 200 1588 Fragments arranged by size: 1588 366 200 BssSI C'ACGA_G Cuts at: 0 742 1647 2154 Size: 742 905 507 Fragments arranged by size: 905 742 507 BstAPI GCAn_nnn'nTGC Cuts at: 0 321 2154 Size: 321 1833 BstXI CCAn_nnnn'nTGG Cuts at: 0 1807 2154 Size: 1807 347 BstYI r'GATC_y Cuts at: 0 1200 1674 2154 Size: 1200 474 480 Fragments arranged by size: 1200 480 474 BtgI C'CryG_G Cuts at: 0 404 431 2154 Size: 404 27 1723 Fragments arranged by size: 1723 404 27 BtsI GCAGTG_nn' Cuts at: 0 389 784 886 2154 Size: 389 395 102 1268 Fragments arranged by size: 1268 395 389 102 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 511 546 2154 Size: 511 35 1608 Fragments arranged by size: 1608 511 35 EaeI y'GGCC_r Cuts at: 0 443 519 611 1743 2154 Size: 443 76 92 1132 411 Fragments arranged by size: 1132 443 411 92 76 EarI CTCTTCn'nnn_ Cuts at: 0 410 1058 1499 2154 Size: 410 648 441 655 Fragments arranged by size: 655 648 441 410 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 85 349 427 588 907 2154 Size: 85 264 78 161 319 1247 Fragments arranged by size: 1247 319 264 161 85 78 EcoICRI GAG'CTC Cuts at: 0 948 1452 1875 2065 2154 Size: 948 504 423 190 89 Fragments arranged by size: 948 504 423 190 89 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 964 1501 1764 2154 Size: 331 633 537 263 390 Fragments arranged by size: 633 537 390 331 263 EcoNI CCTnn'n_nnAGG Cuts at: 0 1682 2154 Size: 1682 472 EcoO109I rG'GnC_Cy Cuts at: 0 106 132 133 182 2090 2154 Size: 106 26 1 49 1908 64 Fragments arranged by size: 1908 106 64 49 26 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1414 1794 2154 Size: 1414 380 360 Fragments arranged by size: 1414 380 360 HaeII r_GCGC'y Cuts at: 0 361 367 373 573 771 1779 2154 Size: 361 6 6 200 198 1008 375 Fragments arranged by size: 1008 375 361 200 198 6 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 490 522 1072 1104 1664 1696 1732 1764 Size: 490 32 550 32 560 32 36 32 Cuts at: 1764 1944 1976 2154 Size: 180 32 178 Fragments arranged by size: 560 550 490 180 178 36 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 675 1758 2154 Size: 675 1083 396 Fragments arranged by size: 1083 675 396 HindIII A'AGCT_T Cuts at: 0 1495 2154 Size: 1495 659 KasI G'GCGC_C Cuts at: 0 363 369 2154 Size: 363 6 1785 Fragments arranged by size: 1785 363 6 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1058 2154 Size: 1058 1096 MscI TGG'CCA Cuts at: 0 521 613 2154 Size: 521 92 1541 Fragments arranged by size: 1541 521 92 MslI CAynn'nnrTG Cuts at: 0 451 881 1892 2154 Size: 451 430 1011 262 Fragments arranged by size: 1011 451 430 262 MspA1I CmG'CkG Cuts at: 0 88 294 309 318 433 666 689 1803 Size: 88 206 15 9 115 233 23 1114 Cuts at: 1803 2154 Size: 351 Fragments arranged by size: 1114 351 233 206 115 88 23 15 9 NaeI GCC'GGC Cuts at: 0 368 2154 Size: 368 1786 NarI GG'CG_CC Cuts at: 0 364 370 2154 Size: 364 6 1784 Fragments arranged by size: 1784 364 6 NgoMIV G'CCGG_C Cuts at: 0 366 2154 Size: 366 1788 NsiI A_TGCA'T Cuts at: 0 1636 2154 Size: 1636 518 NspI r_CATG'y Cuts at: 0 911 1665 2154 Size: 911 754 489 Fragments arranged by size: 911 754 489 PpuMI rG'GwC_Cy Cuts at: 0 182 2090 2154 Size: 182 1908 64 Fragments arranged by size: 1908 182 64 PspOMI G'GGCC_C Cuts at: 0 105 132 2154 Size: 105 27 2022 Fragments arranged by size: 2022 105 27 PspXI vC'TCGA_Gb Cuts at: 0 259 2154 Size: 259 1895 PstI C_TGCA'G Cuts at: 0 129 2154 Size: 129 2025 PvuII CAG'CTG Cuts at: 0 294 309 318 2154 Size: 294 15 9 1836 Fragments arranged by size: 1836 294 15 9 SacI G_AGCT'C Cuts at: 0 950 1454 1877 2067 2154 Size: 950 504 423 190 87 Fragments arranged by size: 950 504 423 190 87 SacII CC_GC'GG Cuts at: 0 434 2154 Size: 434 1720 SfcI C'TryA_G Cuts at: 0 125 2154 Size: 125 2029 SfoI GGC'GCC Cuts at: 0 365 371 2154 Size: 365 6 1783 Fragments arranged by size: 1783 365 6 SgrAI Cr'CCGG_yG Cuts at: 0 366 2154 Size: 366 1788 SmaI CCC'GGG Cuts at: 0 238 2154 Size: 238 1916 SmlI C'TyrA_G Cuts at: 0 259 790 2154 Size: 259 531 1364 Fragments arranged by size: 1364 531 259 StyI C'CwwG_G Cuts at: 0 227 866 1668 2051 2154 Size: 227 639 802 383 103 Fragments arranged by size: 802 639 383 227 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 128 836 2154 Size: 128 708 1318 Fragments arranged by size: 1318 708 128 TatI w'GTAC_w Cuts at: 0 794 1558 2154 Size: 794 764 596 Fragments arranged by size: 794 764 596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2024 2154 Size: 2024 130 XhoI C'TCGA_G Cuts at: 0 259 2154 Size: 259 1895 XmaI C'CCGG_G Cuts at: 0 236 2154 Size: 236 1918 Enzymes that do cut: AccI AcuI AhdI AleI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PpuMI PspOMI PspXI PstI PvuII SacI SacII SfcI SfoI SgrAI SmaI SmlI StyI TaqII TatI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BglII BmgBI BmtI Bpu10I BsaAI BsaBI BsaWI BsiWI BspEI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XmnI ZraI