Restriction map for
pFN21AE3168
(Circular) (Six-base) MAPSORT of: pFN21AE3168.seq Check: 7015 from: 1 to: 5278
pFN21AE3168 5278 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3088 278
Size: 53 83 186 936 1552 2468
Fragments arranged by size:
2468 1552 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 2621 1937
Size: 514 170 4594
Fragments arranged by size:
4594 514 170
Acc65I G'GTAC_C
Cuts at: 2599 2599
Size: 5278
AclI AA'CG_TT
Cuts at: 1563 3405 3778 1563
Size: 1842 373 3063
Fragments arranged by size:
3063 1842 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 3341 4725 4993 1632
Size: 509 1200 1384 268 1917
Fragments arranged by size:
1917 1384 1200 509 268
AfeI AGC'GCT
Cuts at: 2417 2417
Size: 5278
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5081
AflIII A'CryG_T
Cuts at: 1203 4177 5154 1203
Size: 2974 977 1327
Fragments arranged by size:
2974 1327 977
AgeI A'CCGG_T
Cuts at: 4109 4109
Size: 5278
AhdI GACnn_n'nnGTC
Cuts at: 4007 4007
Size: 5278
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5278
AlwNI CAG_nnn'CTG
Cuts at: 3021 4593 3021
Size: 1572 3706
ApaI G_GGCC'C
Cuts at: 2163 2282 2409 2163
Size: 119 127 5032
Fragments arranged by size:
5032 127 119
ApaLI G'TGCA_C
Cuts at: 3335 4491 3335
Size: 1156 4122
ApoI r'AATT_y
Cuts at: 1493 2587 2813 2965 5074 1493
Size: 1094 226 152 2109 1697
Fragments arranged by size:
2109 1697 1094 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3832 160
Size: 872 2800 1606
Fragments arranged by size:
2800 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5278
AvaI C'yCGr_G
Cuts at: 1870 1943 2281 2408 2603 1870
Size: 73 338 127 195 4545
Fragments arranged by size:
4545 338 195 127 73
BamHI G'GATC_C
Cuts at: 2083 2608 2975 2083
Size: 525 367 4386
Fragments arranged by size:
4386 525 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2599 4054 618
Size: 325 217 627 812 1455 1842
Fragments arranged by size:
1842 1455 812 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2163 2220 2282 2336 2353 2409 2460 2597
Size: 772 57 62 54 17 56 51 137
Cuts at: 2597 1391
Size: 4072
Fragments arranged by size:
4072 772 137 62 57 56 54 51
17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2008 2492 928
Size: 971 109 484 3714
Fragments arranged by size:
3714 971 484 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3469 3503 1903
Size: 34 50 34 1448 34 3678
Fragments arranged by size:
3678 1448 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2191 3170 4380 2191
Size: 979 1210 3089
Fragments arranged by size:
3089 1210 979
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3889 136
Size: 107 122 71 631 605 2217 1525
Fragments arranged by size:
2217 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5273 5273
Size: 5278
BlpI GC'TnA_GC
Cuts at: 2695 2695
Size: 5278
Bme1580I G_kGCm'C
Cuts at: 1790 2163 2282 2329 2409 3339 4495 1790
Size: 373 119 47 80 930 1156 2573
Fragments arranged by size:
2573 1156 930 373 119 80 47
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5278
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3009 3967 442
Size: 416 53 83 399 24 1592 958 1753
Fragments arranged by size:
1753 1592 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 3938 1239
Size: 1253 1446 2579
Fragments arranged by size:
2579 1446 1253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3403 4268 4566 3403
Size: 865 298 4115
Fragments arranged by size:
4115 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3941 882
Size: 617 2442 2219
Fragments arranged by size:
2442 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3069 493
Size: 711 1865 2702
Fragments arranged by size:
2702 1865 711
BsaBI GATnn'nnATC
Cuts at: 5272 5272
Size: 5278
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2376 2430 3085 3467
Size: 53 83 186 936 843 54 655 382
Cuts at: 3467 4102 275
Size: 635 1451
Fragments arranged by size:
1451 936 843 655 635 382 186 83
54 53
BsaWI w'CCGG_w
Cuts at: 3711 4109 4383 4530 4941 3711
Size: 398 274 147 411 4048
Fragments arranged by size:
4048 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2465 2495 1178
Size: 30 165 30 1062 30 3961
Fragments arranged by size:
3961 1062 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5278
BseYI C'CCAG_C
Cuts at: 2076 2163 4481 4977 2076
Size: 87 2318 496 2377
Fragments arranged by size:
2377 2318 496 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4705
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2032 2728 3489 4517 1540
Size: 275 186 31 696 761 1028 2301
Fragments arranged by size:
2301 1028 761 696 275 186 31
BsiHKAI G_wGCw'C
Cuts at: 2220 2478 3339 3424 4495 2220
Size: 258 861 85 1071 3003
Fragments arranged by size:
3003 1071 861 258 85
BsmI GAATG_Cn'
Cuts at: 2789 2882 2789
Size: 93 5185
BsmBI CGTCTCn'nnnn_
Cuts at: 2264 2264
Size: 5278
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2220 2282 2329 2336 2353 2409
Size: 399 373 57 62 47 7 17 56
Cuts at: 2409 2460 2478 2597 3339 3424 4495 1391
Size: 51 18 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 119 85 62
57 56 51 47 18 17 7
BspEI T'CCGG_A
Cuts at: 4941 4941
Size: 5278
BspHI T'CATG_A
Cuts at: 3167 4897 3167
Size: 1730 3548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2633 844
Size: 1789 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3165 1394
Size: 429 1342 3507
Fragments arranged by size:
3507 1342 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3773 3947 65
Size: 1183 2525 174 1396
Fragments arranged by size:
2525 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 2361 3922 4109 1700
Size: 110 217 334 1561 187 2869
Fragments arranged by size:
2869 1561 334 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5278
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5278
BssSI C'ACGA_G
Cuts at: 2264 3338 4350 2264
Size: 1074 1012 3192
Fragments arranged by size:
3192 1074 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5278
BstBI TT'CG_AA
Cuts at: 4082 4082
Size: 5278
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5278
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2155
Size: 5278
BstYI r'GATC_y
Cuts at: 1972 2083 2437 2608 2975 3360 3377 4829 4915
Size: 111 354 171 367 385 17 1452 86
Cuts at: 4915 4927 5273 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 354 346 171 111
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2094 2094
Size: 5278
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4726
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4539
Fragments arranged by size:
4539 683 56
BtsI GCAGTG_nn'
Cuts at: 2489 2794 3588 3608 2489
Size: 305 794 20 4159
Fragments arranged by size:
4159 794 305 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5243
DraI TTT'AAA
Cuts at: 2582 2582
Size: 5278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4285 809
Size: 507 2969 1802
Fragments arranged by size:
2969 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2307 2725
Size: 54 1425 50 275 18 199 278 418
Cuts at: 2725 3614 5243 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 418 278 275 199 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2029 2725 1537
Size: 275 217 696 4090
Fragments arranged by size:
4090 696 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 3208 1353
Size: 981 874 3423
Fragments arranged by size:
3423 981 874
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2171 3865 4239 4385 387
Size: 1064 138 582 1694 374 146 1280
Fragments arranged by size:
1694 1280 1064 582 374 146 138
EcoICRI GAG'CTC
Cuts at: 2218 2218
Size: 5278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2492 3341 3938 4725 4993 1239
Size: 393 509 351 849 597 787 268 1524
Fragments arranged by size:
1524 849 787 597 509 393 351 268
EcoO109I rG'GnC_Cy
Cuts at: 2125 2159 2160 2278 2125
Size: 34 1 118 5125
Fragments arranged by size:
5125 118 34 1
EcoRI G'AATT_C
Cuts at: 2587 2965 2587
Size: 378 4900
FspI TGC'GCA
Cuts at: 3784 3784
Size: 5278
HaeII r_GCGC'y
Cuts at: 1678 2419 4425 1678
Size: 741 2006 2531
Fragments arranged by size:
2531 2006 741
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3919 3951 3993 4025 1373
Size: 32 135 32 2347 32 42 32 2626
Fragments arranged by size:
2626 2347 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2622 2868 669
Size: 988 281 684 246 3079
Fragments arranged by size:
3079 988 684 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5278
HpaI GTT'AAC
Cuts at: 2868 2868
Size: 5278
KpnI G_GTAC'C
Cuts at: 2603 2603
Size: 5278
MfeI C'AATT_G
Cuts at: 2877 2877
Size: 5278
MluI A'CGCG_T
Cuts at: 5154 5154
Size: 5278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2278 4392 4576 1305
Size: 973 2114 184 2007
Fragments arranged by size:
2114 2007 973 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3456
Fragments arranged by size:
3456 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2268 3236 3595 3754 518
Size: 648 1102 968 359 159 2042
Fragments arranged by size:
2042 1102 968 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2167 2694 3371 4519 4764 1606
Size: 470 91 527 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 527 470 245 91
NaeI GCC'GGC
Cuts at: 2029 2363 2029
Size: 334 4944
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4726
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5278
NgoMIV G'CCGG_C
Cuts at: 2027 2361 2027
Size: 334 4944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5278
NotI GC'GGCC_GC
Cuts at: 2725 2725
Size: 5278
NspI r_CATG'y
Cuts at: 1132 1229 2636 4181 1132
Size: 97 1407 1545 2229
Fragments arranged by size:
2229 1545 1407 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5278
PciI A'CATG_T
Cuts at: 4177 4177
Size: 5278
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 1108
Size: 1452 3826
PfoI T'CCnGG_A
Cuts at: 2211 2397 2211
Size: 186 5092
PmeI GTTT'AAAC
Cuts at: 2582 2582
Size: 5278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 4860 4892 3347
Size: 32 1481 32 3733
Fragments arranged by size:
3733 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2125
Size: 5278
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5278
PsiI TTA'TAA
Cuts at: 2848 2848
Size: 5278
PspOMI G'GGCC_C
Cuts at: 2159 2278 2405 2159
Size: 119 127 5032
Fragments arranged by size:
5032 127 119
PstI C_TGCA'G
Cuts at: 830 2630 830
Size: 1800 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5278
SacI G_AGCT'C
Cuts at: 2220 2220
Size: 5278
SalI G'TCGA_C
Cuts at: 1936 2620 1936
Size: 684 4594
SbfI CC_TGCA'GG
Cuts at: 2630 2630
Size: 5278
ScaI AGT'ACT
Cuts at: 3526 5238 3526
Size: 1712 3566
SfcI C'TryA_G
Cuts at: 826 1045 2626 3761 4442 4633 826
Size: 219 1581 1135 681 191 1471
Fragments arranged by size:
1581 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5278
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5278
SmaI CCC'GGG
Cuts at: 1872 2283 2605 1872
Size: 411 322 4545
Fragments arranged by size:
4545 411 322
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3382 4283 4545 820
Size: 197 926 1439 901 262 1553
Fragments arranged by size:
1553 1439 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3069 493
Size: 2576 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5278
SphI G_CATG'C
Cuts at: 1132 2636 1132
Size: 1504 3774
SrfI GCCC'GGGC
Cuts at: 2283 2283
Size: 5278
SspI AAT'ATT
Cuts at: 5 52 3202 5
Size: 47 3150 2081
Fragments arranged by size:
3150 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5278
StyI C'CwwG_G
Cuts at: 513 1065 2717 513
Size: 552 1652 3074
Fragments arranged by size:
3074 1652 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2068 2548 3305 3322 3475 3660 4105
Size: 1255 488 480 757 17 153 185 445
Cuts at: 4105 325
Size: 1498
Fragments arranged by size:
1498 1255 757 488 480 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3524 5236 96
Size: 274 80 33 51 1443 1547 1712 138
Fragments arranged by size:
1712 1547 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 5238 5270 2465
Size: 32 2741 32 2473
Fragments arranged by size:
2741 2473 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5278
XbaI T'CTAG_A
Cuts at: 2614 2614
Size: 5278
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5278
XmaI C'CCGG_G
Cuts at: 1870 2281 2603 1870
Size: 411 322 4545
Fragments arranged by size:
4545 411 322
XmnI GAAnn'nnTTC
Cuts at: 3407 3407
Size: 5278
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3086 276
Size: 53 83 186 936 1552 2468
Fragments arranged by size:
2468 1552 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SwaI XcmI