(Linear) (Six-base) MAPSORT of: KIEE3168.seq Check: 7568 from: 1 to: 573 KIEE3168.orf With 182 enzymes: SgfI * July 3, 2012 17:24 .. AccI GT'mk_AC Cuts at: 0 446 573 Size: 446 127 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 573 Size: 136 437 AfeI AGC'GCT Cuts at: 0 412 573 Size: 412 161 ApaI G_GGCC'C Cuts at: 0 158 277 404 573 Size: 158 119 127 169 Fragments arranged by size: 169 158 127 119 AvaI C'yCGr_G Cuts at: 0 276 403 573 Size: 276 127 170 Fragments arranged by size: 276 170 127 BamHI G'GATC_C Cuts at: 0 78 573 Size: 78 495 BanII G_rGCy'C Cuts at: 0 158 215 277 331 348 404 455 573 Size: 158 57 62 54 17 56 51 118 Fragments arranged by size: 158 118 62 57 56 54 51 17 BbsI GAAGACnn'nnnn_ Cuts at: 0 3 487 573 Size: 3 484 86 Fragments arranged by size: 484 86 3 BciVI GTATCCnnnnn_n' Cuts at: 0 186 573 Size: 186 387 Bme1580I G_kGCm'C Cuts at: 0 158 277 324 404 573 Size: 158 119 47 80 169 Fragments arranged by size: 169 158 119 80 47 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 487 573 Size: 487 86 BsaHI Gr'CG_yC Cuts at: 0 371 425 573 Size: 371 54 148 Fragments arranged by size: 371 148 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 460 490 573 Size: 460 30 83 Fragments arranged by size: 460 83 30 BseYI C'CCAG_C Cuts at: 0 71 158 573 Size: 71 87 415 Fragments arranged by size: 415 87 71 BsiEI CG_ry'CG Cuts at: 0 27 573 Size: 27 546 BsiHKAI G_wGCw'C Cuts at: 0 215 473 573 Size: 215 258 100 Fragments arranged by size: 258 215 100 BsmBI CGTCTCn'nnnn_ Cuts at: 0 259 573 Size: 259 314 Bsp1286I G_dGCh'C Cuts at: 0 158 215 277 324 331 348 404 455 Size: 158 57 62 47 7 17 56 51 Cuts at: 455 473 573 Size: 18 100 Fragments arranged by size: 158 100 62 57 56 51 47 18 17 7 BsrFI r'CCGG_y Cuts at: 0 22 356 573 Size: 22 334 217 Fragments arranged by size: 334 217 22 BssSI C'ACGA_G Cuts at: 0 259 573 Size: 259 314 BstXI CCAn_nnnn'nTGG Cuts at: 0 150 573 Size: 150 423 BstYI r'GATC_y Cuts at: 0 78 432 573 Size: 78 354 141 Fragments arranged by size: 354 141 78 Bsu36I CC'TnA_GG Cuts at: 0 89 573 Size: 89 484 BtsI GCAGTG_nn' Cuts at: 0 484 573 Size: 484 89 EaeI y'GGCC_r Cuts at: 0 24 302 573 Size: 24 278 271 Fragments arranged by size: 278 271 24 EagI C'GGCC_G Cuts at: 0 24 573 Size: 24 549 EarI CTCTTCn'nnn_ Cuts at: 0 329 573 Size: 329 244 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 166 573 Size: 166 407 EcoICRI GAG'CTC Cuts at: 0 213 573 Size: 213 360 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 487 573 Size: 136 351 86 Fragments arranged by size: 351 136 86 EcoO109I rG'GnC_Cy Cuts at: 0 120 154 155 273 573 Size: 120 34 1 118 300 Fragments arranged by size: 300 120 118 34 1 HaeII r_GCGC'y Cuts at: 0 414 573 Size: 414 159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 273 573 Size: 273 300 MslI CAynn'nnrTG Cuts at: 0 263 573 Size: 263 310 MspA1I CmG'CkG Cuts at: 0 71 162 573 Size: 71 91 411 Fragments arranged by size: 411 91 71 NaeI GCC'GGC Cuts at: 0 24 358 573 Size: 24 334 215 Fragments arranged by size: 334 215 24 NgoMIV G'CCGG_C Cuts at: 0 22 356 573 Size: 22 334 217 Fragments arranged by size: 334 217 22 PflMI CCAn_nnn'nTGG Cuts at: 0 555 573 Size: 555 18 PfoI T'CCnGG_A Cuts at: 0 206 392 573 Size: 206 186 181 Fragments arranged by size: 206 186 181 PpuMI rG'GwC_Cy Cuts at: 0 120 573 Size: 120 453 PspOMI G'GGCC_C Cuts at: 0 154 273 400 573 Size: 154 119 127 173 Fragments arranged by size: 173 154 127 119 SacI G_AGCT'C Cuts at: 0 215 573 Size: 215 358 SmaI CCC'GGG Cuts at: 0 278 573 Size: 278 295 SrfI GCCC'GGGC Cuts at: 0 278 573 Size: 278 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 63 543 573 Size: 63 480 30 Fragments arranged by size: 480 63 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 460 492 573 Size: 460 32 81 Fragments arranged by size: 460 81 32 XmaI C'CCGG_G Cuts at: 0 276 573 Size: 276 297 Enzymes that do cut: AccI AcuI AfeI ApaI AvaI BamHI BanII BbsI BciVI Bme1580I BpmI BsaHI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssSI BstXI BstYI Bsu36I BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII MmeI MslI MspA1I NaeI NgoMIV PflMI PfoI PpuMI PspOMI SacI SmaI SrfI TaqII TstI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI