Restriction map for
pFN21AE1589
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AflII AhdI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BcgI BciVI BfrI BglI
BglII BlnI BsgI BsiWI BspDI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO109I EcoO65I
EcoRV FseI FspI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NarI NdeI
NheI NotI NruI NspV PacI
PflFI PmeI PpuMI PshAI PshBI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 748 GTCTAC GT^[AC][GT]AC
AccIII 2113 TCCGGA T^CCGGA
AclI 80 AACGTT AA^CGTT
AcyI 307 GGCGTC G[GA]^CG[CT]C
AflIII 1363 ACATGT A^C[GA][CT]GT
AgeI 2753 ACCGGT A^CCGGT
AlwNI 2600 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2468 AGCGCT AGC^GCT
BanI 990 GGCACC G^G[CT][GA]CC
BbrPI 295 CACGTG CAC^GTG
BclI 480 TGATCA T^GATCA
BsaAI 295 CACGTG [CT]AC^GT[GA]
BsaBI 1303 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 307 GGCGTC G[GA]^CG[CT]C
BseAI 2113 TCCGGA T^CCGGA
BspEI 2113 TCCGGA T^CCGGA
BspHI 3023 TCATGA T^CATGA
BspLU11I 1363 ACATGT A^CATGT
DraI 606 TTTAAA TTT^AAA
DsaI 2264 CCATGG C^C[GA][CT]GG
Eco47III 2468 AGCGCT AGC^GCT
EcoRI 374 GAATTC G^AATTC
EcoT14I 2264 CCATGG C^C[AT][AT]GG
EcoT22I 1849 ATGCAT ATGCA^T
FauI 549 GCGGG CCCGC(4/6)
FbaI 480 TGATCA T^GATCA
HaeII 2468 AGCGCT [GA]GCGC^[CT]
Hin1I 307 GGCGTC G[GA]^CG[CT]C
MamI 1303 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2113 TCCGGA T^CCGGA
MspA1I 5 CAGCTG C[AC]G^C[GT]G
NaeI 1043 GCCGGC GCC^GGC
NcoI 2264 CCATGG C^CATGG
NgoMIV 1043 GCCGGC G^CCGGC
NsiI 1849 ATGCAT ATGCA^T
PaeR7I 2519 CTCGAG C^TCGAG
PflMI 2415 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2753 ACCGGT A^CCGGT
PmaCI 295 CACGTG CAC^GTG
PmlI 295 CACGTG CAC^GTG
Psp1406I 80 AACGTT AA^CGTT
PstI 2606 CTGCAG CTGCA^G
PvuII 5 CAGCTG CAG^CTG
RcaI 3023 TCATGA T^CATGA
SexAI 2225 ACCAGGT A^CC[AT]GGT
SphI 1148 GCATGC GCATG^C
StyI 2264 CCATGG C^C[AT][AT]GG
Van91I 2415 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 346 CCACCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2519 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 965 GTGCAC G^TGCAC
1503 GTGCAC
AocI 2332 CCTCAGG CC^T[ACGT]AGG
2880 CCTGAGG
ApaLI 965 GTGCAC G^TGCAC
1503 GTGCAC
AvaI 970 CCCGAG C^[CT]CG[GA]G
2519 CTCGAG
BalI 1127 TGGCCA TGG^CCA
2894 TGGCCA
BanII 2639 GAGCTC G[GA]GC[CT]^C
2986 GAGCTC
BcnI 2152 CCGGG CC^[GC]GG
2644 CCCGG
BsaI 2908 GAGACC GGTCTC(1/5)
3147 GAGACC
BsaWI 2113 TCCGGA [AT]^CCGG[AT]
2753 ACCGGT
BsiEI 1896 CGGTCG CG[GA][CT]^CG
2755 CGGTCG
BsmBI 309 CGTCTC CGTCTC(1/5)
1164 CGTCTC
BsmI 766 GAATGC GAATGC(1/-1)
941 GAATGC
BsoBI 970 CCCGAG C^[CT]CG[GA]G
2519 CTCGAG
Bsp1407I 1305 TGTACA T^GTACA
1626 TGTACA
BsrFI 1043 GCCGGC [GA]^CCGG[CT]
2753 ACCGGT
BsrGI 1305 TGTACA T^GTACA
1626 TGTACA
BstAPI 1143 GCAAGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2400 GCAAGTGTTGC
Bsu36I 2332 CCTCAGG CC^T[ACGT]AGG
2880 CCTGAGG
CfoI 2067 GCGC GCG^C
2469 GCGC
Cfr10I 1043 GCCGGC [GA]^CCGG[CT]
2753 ACCGGT
EaeI 1127 TGGCCA [CT]^GGCC[GA]
2894 TGGCCA
Eco81I 2332 CCTCAGG CC^T[ACGT]AGG
2880 CCTGAGG
EcoNI 1229 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1864 CCTTTTCAAGG
HhaI 2067 GCGC GCG^C
2469 GCGC
HinP1I 2067 GCGC G^CGC
2469 GCGC
HindIII 1733 AAGCTT A^AGCTT
2707 AAGCTT
MluNI 1127 TGGCCA TGG^CCA
2894 TGGCCA
MscI 1127 TGGCCA TGG^CCA
2894 TGGCCA
NciI 2152 CCGGG CC^[GC]GG
2644 CCCGG
SacI 2639 GAGCTC GAGCT^C
2986 GAGCTC
ScaI 49 AGTACT AGT^ACT
1720 AGTACT
SmlI 890 CTCAAG C^T[CT][GA]AG
2519 CTCGAG
SspBI 1305 TGTACA T^GTACA
1626 TGTACA
XbaI 519 TCTAGA T^CTAGA
1608 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbsI
BbvCI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI