(Circular) (Six-base) MAPSORT of: pFN21AE3466.seq Check: 4256 from: 1 to: 8875 pFN21AE3466 8875 bp With 182 enzymes: SgfI * August 1, 2019 17:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6685 278 Size: 53 83 186 936 5149 2468 Fragments arranged by size: 5149 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2370 5007 6218 1937 Size: 433 2637 1211 4594 Fragments arranged by size: 4594 2637 1211 433 Acc65I G'GTAC_C Cuts at: 6196 6196 Size: 8875 AclI AA'CG_TT Cuts at: 1563 7002 7375 1563 Size: 5439 373 3063 Fragments arranged by size: 5439 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2222 2939 4195 4626 4692 6938 8322 8590 Size: 590 717 1256 431 66 2246 1384 268 Cuts at: 8590 1632 Size: 1917 Fragments arranged by size: 2246 1917 1384 1256 717 590 431 268 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8678 AflIII A'CryG_T Cuts at: 1203 2434 4091 5094 5688 7774 8751 1203 Size: 1231 1657 1003 594 2086 977 1327 Fragments arranged by size: 2086 1657 1327 1231 1003 977 594 AgeI A'CCGG_T Cuts at: 2574 7706 2574 Size: 5132 3743 AhdI GACnn_n'nnGTC Cuts at: 2467 7604 2467 Size: 5137 3738 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2057 1184 Size: 873 8002 AlwNI CAG_nnn'CTG Cuts at: 2847 3062 6618 8190 2847 Size: 215 3556 1572 3532 Fragments arranged by size: 3556 3532 1572 215 ApaI G_GGCC'C Cuts at: 2986 5223 2986 Size: 2237 6638 ApaLI G'TGCA_C Cuts at: 3720 6932 8088 3720 Size: 3212 1156 4507 Fragments arranged by size: 4507 3212 1156 ApoI r'AATT_y Cuts at: 1493 2608 3217 3436 3788 4060 4242 4306 4792 Size: 1115 609 219 352 272 182 64 486 Cuts at: 4792 5316 5503 5648 6184 6410 6562 8671 1493 Size: 524 187 145 536 226 152 2109 1697 Fragments arranged by size: 2109 1697 1115 609 536 524 486 352 272 226 219 187 182 152 145 64 AseI AT'TA_AT Cuts at: 160 1032 7429 160 Size: 872 6397 1606 Fragments arranged by size: 6397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8875 AvaI C'yCGr_G Cuts at: 1870 1943 4321 6200 1870 Size: 73 2378 1879 4545 Fragments arranged by size: 4545 2378 1879 73 BamHI G'GATC_C Cuts at: 3970 4479 6205 6572 3970 Size: 509 1726 367 6273 Fragments arranged by size: 6273 1726 509 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2198 3624 5964 6196 7651 Size: 325 217 627 411 1426 2340 232 1455 Cuts at: 7651 618 Size: 1842 Fragments arranged by size: 2340 1842 1455 1426 627 411 325 232 217 BanII G_rGCy'C Cuts at: 1391 2511 2986 5223 5848 6194 1391 Size: 1120 475 2237 625 346 4072 Fragments arranged by size: 4072 2237 1120 625 475 346 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2238 5552 5911 928 Size: 971 339 3314 359 3892 Fragments arranged by size: 3892 3314 971 359 339 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7066 7100 1903 Size: 34 5129 34 3678 Fragments arranged by size: 5129 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5091 6108 6767 7977 5091 Size: 1017 659 1210 5989 Fragments arranged by size: 5989 1210 1017 659 BclI T'GATC_A Cuts at: 1548 2386 1548 Size: 838 8037 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 7486 136 Size: 107 122 71 631 605 337 5477 1525 Fragments arranged by size: 5477 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3681 4891 5494 5542 8870 3681 Size: 1210 603 48 3328 3686 Fragments arranged by size: 3686 3328 1210 603 48 BlpI GC'TnA_GC Cuts at: 6292 6292 Size: 8875 Bme1580I G_kGCm'C Cuts at: 1790 2986 3724 4963 5223 6936 8092 1790 Size: 1196 738 1239 260 1713 1156 2573 Fragments arranged by size: 2573 1713 1239 1196 1156 738 260 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2271 3531 5217 Size: 416 53 83 399 24 854 1260 1686 Cuts at: 5217 6606 7564 442 Size: 1389 958 1753 Fragments arranged by size: 1753 1686 1389 1260 958 854 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2912 3146 3450 7535 1239 Size: 1673 234 304 4085 2579 Fragments arranged by size: 4085 2579 1673 304 234 Bpu10I CC'TnA_GC Cuts at: 3659 3659 Size: 8875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7000 7865 8163 7000 Size: 865 298 7712 Fragments arranged by size: 7712 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3342 5463 7538 882 Size: 617 1843 2121 2075 2219 Fragments arranged by size: 2219 2121 2075 1843 617 BsaAI yAC'GTr Cuts at: 493 1204 6666 493 Size: 711 5462 2702 Fragments arranged by size: 5462 2702 711 BsaBI GATnn'nnATC Cuts at: 3249 8869 3249 Size: 5620 3255 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6682 7064 7699 275 Size: 53 83 186 936 5149 382 635 1451 Fragments arranged by size: 5149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2574 7308 7706 7980 8127 8538 2574 Size: 4734 398 274 147 411 2911 Fragments arranged by size: 4734 2911 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2538 2568 1178 Size: 30 165 30 1135 30 7485 Fragments arranged by size: 7485 1135 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2080 4926 5810 5856 1186 Size: 894 2846 884 46 4205 Fragments arranged by size: 4205 2846 894 884 46 BseYI C'CCAG_C Cuts at: 2510 2985 3759 4138 8078 8574 2510 Size: 475 774 379 3940 496 2811 Fragments arranged by size: 3940 2811 774 496 475 379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 3817 5096 5222 1163 Size: 573 345 1736 1279 126 4816 Fragments arranged by size: 4816 1736 1279 573 345 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3647 6325 7086 8114 1540 Size: 275 186 1646 2678 761 1028 2301 Fragments arranged by size: 2678 2301 1646 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 3192 3724 3857 4280 5176 5820 6048 6936 Size: 1146 532 133 423 896 644 228 888 Cuts at: 6936 7021 8092 2046 Size: 85 1071 2829 Fragments arranged by size: 2829 1146 1071 896 888 644 532 423 228 133 85 BsmI GAATG_Cn' Cuts at: 2638 2869 3095 3659 6386 6479 2638 Size: 231 226 564 2727 93 5034 Fragments arranged by size: 5034 2727 564 231 226 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2511 2986 3192 3724 3857 4280 Size: 399 256 465 475 206 532 133 423 Cuts at: 4280 4963 5176 5223 5820 5848 6048 6194 6936 Size: 683 213 47 597 28 200 146 742 Cuts at: 6936 7021 8092 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 683 597 532 475 465 423 399 256 213 206 200 146 133 85 47 28 BspEI T'CCGG_A Cuts at: 8538 8538 Size: 8875 BspHI T'CATG_A Cuts at: 3184 5394 6764 8494 3184 Size: 2210 1370 1730 3565 Fragments arranged by size: 3565 2210 1730 1370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 3385 4710 6230 844 Size: 1588 953 1325 1520 3489 Fragments arranged by size: 3489 1588 1520 1325 953 BsrBI CCG'CTC Cuts at: 1394 1823 3663 6762 1394 Size: 429 1840 3099 3507 Fragments arranged by size: 3507 3099 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2077 2633 4570 7370 7544 65 Size: 1183 829 556 1937 2800 174 1396 Fragments arranged by size: 2800 1937 1396 1183 829 556 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2574 7519 7706 1700 Size: 110 764 4945 187 2869 Fragments arranged by size: 4945 2869 764 187 110 BsrGI T'GTAC_A Cuts at: 96 4510 5713 96 Size: 4414 1203 3258 Fragments arranged by size: 4414 3258 1203 BssHII G'CGCG_C Cuts at: 1925 3236 1925 Size: 1311 7564 BssSI C'ACGA_G Cuts at: 2039 6935 7947 2039 Size: 4896 1012 2967 Fragments arranged by size: 4896 2967 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3588 794 Size: 2794 6081 BstBI TT'CG_AA Cuts at: 5500 5512 7679 5500 Size: 12 2167 6696 Fragments arranged by size: 6696 2167 12 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8875 BstXI CCAn_nnnn'nTGG Cuts at: 5748 5748 Size: 8875 BstYI r'GATC_y Cuts at: 1972 2924 3540 3681 3970 4225 4479 4891 5494 Size: 952 616 141 289 255 254 412 603 Cuts at: 5494 5542 6205 6572 6957 6974 8426 8512 8524 Size: 48 663 367 385 17 1452 86 12 Cuts at: 8524 8870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 952 663 616 603 412 385 367 346 289 255 254 141 86 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2145 3934 513 Size: 552 1080 1789 5454 Fragments arranged by size: 5454 1789 1080 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4364 487 Size: 683 56 3138 4998 Fragments arranged by size: 4998 3138 683 56 BtsI GCAGTG_nn' Cuts at: 2479 3190 4906 5205 6391 7185 7205 2479 Size: 711 1716 299 1186 794 20 4149 Fragments arranged by size: 4149 1716 1186 794 711 299 20 ClaI AT'CG_AT Cuts at: 1554 3079 1554 Size: 1525 7350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8840 DraI TTT'AAA Cuts at: 2223 3798 4101 6179 2223 Size: 1575 303 2078 4919 Fragments arranged by size: 4919 2078 1575 303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2698 5095 7882 809 Size: 507 1382 2397 2787 1802 Fragments arranged by size: 2787 2397 1802 1382 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3931 6322 7211 Size: 54 1425 50 275 18 2101 2391 889 Cuts at: 7211 8840 8 Size: 1629 43 Fragments arranged by size: 2391 2101 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6322 1537 Size: 275 4510 4090 Fragments arranged by size: 4510 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2499 5254 6805 1353 Size: 1146 2755 1551 3423 Fragments arranged by size: 3423 2755 1551 1146 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7462 7836 7982 387 Size: 1064 138 5873 374 146 1280 Fragments arranged by size: 5873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2912 2939 3146 3450 4195 4626 Size: 393 590 690 27 207 304 745 431 Cuts at: 4626 4692 6938 7535 8322 8590 1239 Size: 66 2246 597 787 268 1524 Fragments arranged by size: 2246 1524 787 745 690 597 590 431 393 304 268 207 66 27 EcoNI CCTnn'n_nnAGG Cuts at: 2240 2240 Size: 8875 EcoO109I rG'GnC_Cy Cuts at: 2011 2421 2982 2011 Size: 410 561 7904 Fragments arranged by size: 7904 561 410 EcoRI G'AATT_C Cuts at: 4060 4306 6184 6562 4060 Size: 246 1878 378 6373 Fragments arranged by size: 6373 1878 378 246 EcoRV GAT'ATC Cuts at: 2646 6015 2646 Size: 3369 5506 FspI TGC'GCA Cuts at: 7381 7381 Size: 8875 HaeII r_GCGC'y Cuts at: 1678 2541 8022 1678 Size: 863 5481 2531 Fragments arranged by size: 5481 2531 863 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 2538 2570 3658 Size: 32 135 32 881 32 53 32 1088 Cuts at: 3658 3690 5512 5544 5789 5821 7516 7548 7590 Size: 32 1822 32 245 32 1695 32 42 Cuts at: 7590 7622 1373 Size: 32 2626 Fragments arranged by size: 2626 1822 1695 1088 881 245 135 53 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2371 2413 3115 3139 3286 4376 Size: 988 281 433 42 702 24 147 1090 Cuts at: 4376 6219 6465 669 Size: 1843 246 3079 Fragments arranged by size: 3079 1843 1090 988 702 433 281 246 147 42 24 HindIII A'AGCT_T Cuts at: 748 748 Size: 8875 HpaI GTT'AAC Cuts at: 6465 6465 Size: 8875 KpnI G_GTAC'C Cuts at: 6200 6200 Size: 8875 MfeI C'AATT_G Cuts at: 4077 6474 4077 Size: 2397 6478 MluI A'CGCG_T Cuts at: 8751 8751 Size: 8875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2451 4274 4615 5277 5555 7989 8173 1305 Size: 1146 1823 341 662 278 2434 184 2007 Fragments arranged by size: 2434 2007 1823 1146 662 341 278 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3933 10 Size: 54 1425 343 2101 4952 Fragments arranged by size: 4952 2101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2354 2882 3871 4337 5244 5415 Size: 648 873 315 528 989 466 907 171 Cuts at: 5415 6833 7192 7351 518 Size: 1418 359 159 2042 Fragments arranged by size: 2042 1418 989 907 873 648 528 466 359 315 171 159 MspA1I CmG'CkG Cuts at: 1606 2018 2147 4429 5464 6291 6968 8116 8361 Size: 412 129 2282 1035 827 677 1148 245 Cuts at: 8361 1606 Size: 2120 Fragments arranged by size: 2282 2120 1148 1035 827 677 412 245 129 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8323 NdeI CA'TA_TG Cuts at: 387 3752 387 Size: 3365 5510 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8875 NotI GC'GGCC_GC Cuts at: 6322 6322 Size: 8875 NspI r_CATG'y Cuts at: 1132 1229 2438 4095 4374 5098 5210 5692 6233 Size: 97 1209 1657 279 724 112 482 541 Cuts at: 6233 7778 1132 Size: 1545 2229 Fragments arranged by size: 2229 1657 1545 1209 724 541 482 279 112 97 PasI CC'CwG_GG Cuts at: 1348 2542 1348 Size: 1194 7681 PciI A'CATG_T Cuts at: 2434 4091 5094 5688 7774 2434 Size: 1657 1003 594 2086 3535 Fragments arranged by size: 3535 2086 1657 1003 594 PflMI CCAn_nnn'nTGG Cuts at: 1108 3551 1108 Size: 2443 6432 PfoI T'CCnGG_A Cuts at: 3444 6008 3444 Size: 2564 6311 PmeI GTTT'AAAC Cuts at: 6179 6179 Size: 8875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6944 6976 8457 8489 6944 Size: 32 1481 32 7330 Fragments arranged by size: 7330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2421 2421 Size: 8875 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8875 PsiI TTA'TAA Cuts at: 6445 6445 Size: 8875 PspOMI G'GGCC_C Cuts at: 2982 5219 2982 Size: 2237 6638 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3247 3279 3247 Size: 32 8843 PstI C_TGCA'G Cuts at: 830 2309 3566 3584 6227 830 Size: 1479 1257 18 2643 3478 Fragments arranged by size: 3478 2643 1479 1257 18 PvuI CG_AT'CG Cuts at: 2001 3647 2001 Size: 1646 7229 PvuII CAG'CTG Cuts at: 4429 4429 Size: 8875 SacII CC_GC'GG Cuts at: 2148 2148 Size: 8875 SalI G'TCGA_C Cuts at: 1936 2369 6217 1936 Size: 433 3848 4594 Fragments arranged by size: 4594 3848 433 SapI GCTCTTCn'nnn_ Cuts at: 2499 2499 Size: 8875 SbfI CC_TGCA'GG Cuts at: 6227 6227 Size: 8875 ScaI AGT'ACT Cuts at: 7123 8835 7123 Size: 1712 7163 SfcI C'TryA_G Cuts at: 826 1045 2305 3562 3580 4980 5004 5707 6223 Size: 219 1260 1257 18 1400 24 703 516 Cuts at: 6223 7358 8039 8230 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1400 1260 1257 1135 703 681 516 219 191 24 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8875 SmaI CCC'GGG Cuts at: 1872 6202 1872 Size: 4330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6979 7880 8142 820 Size: 197 926 5036 901 262 1553 Fragments arranged by size: 5036 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6666 493 Size: 6173 2702 SpeI A'CTAG_T Cuts at: 152 5111 152 Size: 4959 3916 SphI G_CATG'C Cuts at: 1132 6233 1132 Size: 5101 3774 SspI AAT'ATT Cuts at: 5 52 6799 5 Size: 47 6747 2081 Fragments arranged by size: 6747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8875 StyI C'CwwG_G Cuts at: 513 1065 2055 2194 3817 4762 6314 513 Size: 552 990 139 1623 945 1552 3074 Fragments arranged by size: 3074 1623 1552 990 945 552 139 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 2295 5396 6902 6919 7072 7257 Size: 1255 590 125 3101 1506 17 153 185 Cuts at: 7257 7702 325 Size: 445 1498 Fragments arranged by size: 3101 1506 1498 1255 590 445 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 3953 4094 Size: 274 80 33 51 1443 276 1700 141 Cuts at: 4094 4510 4573 4927 5713 7121 8833 96 Size: 416 63 354 786 1408 1712 138 Fragments arranged by size: 1712 1700 1443 1408 786 416 354 276 274 141 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 3868 3900 8835 8867 2019 Size: 32 1817 32 4935 32 2027 Fragments arranged by size: 4935 2027 1817 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8875 XbaI T'CTAG_A Cuts at: 2809 4064 6211 2809 Size: 1255 2147 5473 Fragments arranged by size: 5473 2147 1255 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6159 6159 Size: 8875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8875 XmaI C'CCGG_G Cuts at: 1870 6200 1870 Size: 4330 4545 XmnI GAAnn'nnTTC Cuts at: 2235 4908 7004 2235 Size: 2673 2096 4106 Fragments arranged by size: 4106 2673 2096 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6683 276 Size: 53 83 186 936 5149 2468 Fragments arranged by size: 5149 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI