(Circular) (Six-base) MAPSORT of: pFN21AE4055.seq Check: 1638 from: 1 to: 7366 pFN21AE4055 7366 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5176 278 Size: 53 83 186 936 3640 2468 Fragments arranged by size: 3640 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2036 2319 3381 4709 1937 Size: 99 283 1062 1328 4594 Fragments arranged by size: 4594 1328 1062 283 99 Acc65I G'GTAC_C Cuts at: 2270 4687 2270 Size: 2417 4949 AclI AA'CG_TT Cuts at: 1563 3114 3240 5493 5866 1563 Size: 1551 126 2253 373 3063 Fragments arranged by size: 3063 2253 1551 373 126 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2381 2916 2984 3152 4205 4448 4656 5429 Size: 749 535 68 168 1053 243 208 773 Cuts at: 5429 6813 7081 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1053 773 749 535 268 243 208 168 68 AflII C'TTAA_G Cuts at: 820 1017 2816 820 Size: 197 1799 5370 Fragments arranged by size: 5370 1799 197 AflIII A'CryG_T Cuts at: 1203 6265 7242 1203 Size: 5062 977 1327 Fragments arranged by size: 5062 1327 977 AgeI A'CCGG_T Cuts at: 6197 6197 Size: 7366 AhdI GACnn_n'nnGTC Cuts at: 6095 6095 Size: 7366 AleI CACnn'nnGTG Cuts at: 1166 2965 1166 Size: 1799 5567 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4140 1184 Size: 2956 4410 AlwNI CAG_nnn'CTG Cuts at: 2894 5109 6681 2894 Size: 2215 1572 3579 Fragments arranged by size: 3579 2215 1572 ApaLI G'TGCA_C Cuts at: 5423 6579 5423 Size: 1156 6210 ApoI r'AATT_y Cuts at: 1493 2060 2832 3428 3581 4675 4901 5053 7162 Size: 567 772 596 153 1094 226 152 2109 Cuts at: 7162 1493 Size: 1697 Fragments arranged by size: 2109 1697 1094 772 596 567 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 5920 160 Size: 872 4888 1606 Fragments arranged by size: 4888 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7366 AvaI C'yCGr_G Cuts at: 1870 1943 3123 4691 1870 Size: 73 1180 1568 4545 Fragments arranged by size: 4545 1568 1180 73 BamHI G'GATC_C Cuts at: 4696 5063 4696 Size: 367 6999 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2270 4687 6142 618 Size: 325 217 627 483 2417 1455 1842 Fragments arranged by size: 2417 1842 1455 627 483 325 217 BanII G_rGCy'C Cuts at: 1391 2194 2864 4685 1391 Size: 803 670 1821 4072 Fragments arranged by size: 4072 1821 803 670 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3671 4293 4352 928 Size: 971 1772 622 59 3942 Fragments arranged by size: 3942 1772 971 622 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5557 5591 1903 Size: 34 3620 34 3678 Fragments arranged by size: 3678 3620 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2138 5258 6468 2138 Size: 3120 1210 3036 Fragments arranged by size: 3120 3036 1210 BclI T'GATC_A Cuts at: 1548 2065 3412 3721 3952 1548 Size: 517 1347 309 231 4962 Fragments arranged by size: 4962 1347 517 309 231 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5977 136 Size: 107 122 71 631 605 4305 1525 Fragments arranged by size: 4305 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3826 7361 3826 Size: 3535 3831 BlpI GC'TnA_GC Cuts at: 4783 4783 Size: 7366 Bme1580I G_kGCm'C Cuts at: 1790 5427 6583 1790 Size: 3637 1156 2573 Fragments arranged by size: 3637 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5097 6055 442 Size: 416 53 83 399 24 3680 958 1753 Fragments arranged by size: 3680 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7366 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3654 6026 1239 Size: 2415 2372 2579 Fragments arranged by size: 2579 2415 2372 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2178 3264 4278 5491 6356 6654 2178 Size: 1086 1014 1213 865 298 2890 Fragments arranged by size: 2890 1213 1086 1014 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6029 882 Size: 617 4530 2219 Fragments arranged by size: 4530 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3385 5157 493 Size: 711 2181 1772 2702 Fragments arranged by size: 2702 2181 1772 711 BsaBI GATnn'nnATC Cuts at: 3546 3720 7360 3546 Size: 174 3640 3552 Fragments arranged by size: 3640 3552 174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5173 5555 6190 275 Size: 53 83 186 936 3640 382 635 1451 Fragments arranged by size: 3640 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5799 6197 6471 6618 7029 5799 Size: 398 274 147 411 6136 Fragments arranged by size: 6136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2851 2881 3024 3054 1178 Size: 30 165 30 1448 30 143 30 5490 Fragments arranged by size: 5490 1448 165 143 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7366 BseYI C'CCAG_C Cuts at: 4305 6569 7065 4305 Size: 2264 496 4606 Fragments arranged by size: 4606 2264 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6793 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3288 4816 5577 6605 1540 Size: 275 186 1287 1528 761 1028 2301 Fragments arranged by size: 2301 1528 1287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2194 3763 5427 5512 6583 2194 Size: 1569 1664 85 1071 2977 Fragments arranged by size: 2977 1664 1569 1071 85 BsmI GAATG_Cn' Cuts at: 3558 3804 4877 4970 3558 Size: 246 1073 93 5954 Fragments arranged by size: 5954 1073 246 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4481 4481 Size: 7366 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2194 2864 3763 4685 5427 5512 6583 Size: 399 404 670 899 922 742 85 1071 Cuts at: 6583 1391 Size: 2174 Fragments arranged by size: 2174 1071 922 899 742 670 404 399 85 BspEI T'CCGG_A Cuts at: 7029 7029 Size: 7366 BspHI T'CATG_A Cuts at: 2841 5255 6985 2841 Size: 2414 1730 3222 Fragments arranged by size: 3222 2414 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4108 4721 844 Size: 3264 613 3489 Fragments arranged by size: 3489 3264 613 BsrBI CCG'CTC Cuts at: 1394 1823 5253 1394 Size: 429 3430 3507 Fragments arranged by size: 3507 3430 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5861 6035 65 Size: 1183 4613 174 1396 Fragments arranged by size: 4613 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4038 6010 6197 1700 Size: 110 2228 1972 187 2869 Fragments arranged by size: 2869 2228 1972 187 110 BsrGI T'GTAC_A Cuts at: 96 4015 96 Size: 3919 3447 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7366 BssSI C'ACGA_G Cuts at: 5426 6438 5426 Size: 1012 6354 BstAPI GCAn_nnn'nTGC Cuts at: 794 2648 794 Size: 1854 5512 BstBI TT'CG_AA Cuts at: 6170 6170 Size: 7366 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7366 BstXI CCAn_nnnn'nTGG Cuts at: 4201 4201 Size: 7366 BstYI r'GATC_y Cuts at: 1972 2154 2221 2230 3088 3826 3917 4696 5063 Size: 182 67 9 858 738 91 779 367 Cuts at: 5063 5448 5465 6917 7003 7015 7361 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 858 779 738 385 367 346 182 91 86 67 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2150 2150 Size: 7366 BtgI C'CryG_G Cuts at: 513 1065 4512 513 Size: 552 3447 3367 Fragments arranged by size: 3447 3367 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6627 Fragments arranged by size: 6627 683 56 BtsI GCAGTG_nn' Cuts at: 3074 3338 4146 4614 4882 5676 5696 3074 Size: 264 808 468 268 794 20 4744 Fragments arranged by size: 4744 808 794 468 268 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7366 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7331 DraI TTT'AAA Cuts at: 2017 3027 4670 2017 Size: 1010 1643 4713 Fragments arranged by size: 4713 1643 1010 DraIII CAC_nnn'GTG Cuts at: 3203 3203 Size: 7366 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4354 6373 809 Size: 507 3038 2019 1802 Fragments arranged by size: 3038 2019 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4471 4813 5702 Size: 54 1425 50 275 18 2641 342 889 Cuts at: 5702 7331 8 Size: 1629 43 Fragments arranged by size: 2641 1629 1425 889 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4813 1537 Size: 275 3001 4090 Fragments arranged by size: 4090 3001 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2599 3695 4083 4181 4355 5296 1353 Size: 1246 1096 388 98 174 941 3423 Fragments arranged by size: 3423 1246 1096 941 388 174 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5953 6327 6473 387 Size: 1064 138 4364 374 146 1280 Fragments arranged by size: 4364 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2192 2192 Size: 7366 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2916 2984 3152 3654 4205 4448 Size: 393 749 535 68 168 502 551 243 Cuts at: 4448 4656 5429 6026 6813 7081 1239 Size: 208 773 597 787 268 1524 Fragments arranged by size: 1524 787 773 749 597 551 535 502 393 268 243 208 168 68 EcoRI G'AATT_C Cuts at: 4675 5053 4675 Size: 378 6988 EcoRV GAT'ATC Cuts at: 3800 3800 Size: 7366 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2225 2225 Size: 7366 FspI TGC'GCA Cuts at: 5872 5872 Size: 7366 HaeII r_GCGC'y Cuts at: 1678 6513 1678 Size: 4835 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2908 2940 3024 Size: 32 135 32 446 32 858 32 84 Cuts at: 3024 3028 3056 3060 3397 3429 3940 3972 4445 Size: 4 28 4 337 32 511 32 473 Cuts at: 4445 4477 6007 6039 6081 6113 1373 Size: 32 1530 32 42 32 2626 Fragments arranged by size: 2626 1530 858 511 473 446 337 135 84 42 32 32 32 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2095 3190 4710 4956 669 Size: 988 281 157 1095 1520 246 3079 Fragments arranged by size: 3079 1520 1095 988 281 246 157 HindIII A'AGCT_T Cuts at: 748 2849 3460 4232 748 Size: 2101 611 772 3882 Fragments arranged by size: 3882 2101 772 611 HpaI GTT'AAC Cuts at: 3190 4956 3190 Size: 1766 5600 KpnI G_GTAC'C Cuts at: 2274 4691 2274 Size: 2417 4949 MfeI C'AATT_G Cuts at: 4965 4965 Size: 7366 MluI A'CGCG_T Cuts at: 7242 7242 Size: 7366 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6480 6664 1305 Size: 5175 184 2007 Fragments arranged by size: 5175 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5544 Fragments arranged by size: 5544 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2965 4517 5324 5683 5842 518 Size: 648 1799 1552 807 359 159 2042 Fragments arranged by size: 2042 1799 1552 807 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4399 4782 5459 6607 6852 1606 Size: 2793 383 677 1148 245 2120 Fragments arranged by size: 2793 2120 1148 677 383 245 NaeI GCC'GGC Cuts at: 4040 4040 Size: 7366 NcoI C'CATG_G Cuts at: 513 1065 4512 513 Size: 552 3447 3367 Fragments arranged by size: 3447 3367 552 NdeI CA'TA_TG Cuts at: 387 2747 387 Size: 2360 5006 NgoMIV G'CCGG_C Cuts at: 4038 4038 Size: 7366 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7366 NotI GC'GGCC_GC Cuts at: 4813 4813 Size: 7366 NspI r_CATG'y Cuts at: 1132 1229 2938 3906 4724 6269 1132 Size: 97 1709 968 818 1545 2229 Fragments arranged by size: 2229 1709 1545 968 818 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7366 PciI A'CATG_T Cuts at: 6265 6265 Size: 7366 PflMI CCAn_nnn'nTGG Cuts at: 1108 2473 2608 1108 Size: 1365 135 5866 Fragments arranged by size: 5866 1365 135 PmeI GTTT'AAAC Cuts at: 4670 4670 Size: 7366 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5435 5467 6948 6980 5435 Size: 32 1481 32 5821 Fragments arranged by size: 5821 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4514 1613 Size: 2901 4465 PsiI TTA'TAA Cuts at: 2619 4936 2619 Size: 2317 5049 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2893 2925 2893 Size: 32 7334 PstI C_TGCA'G Cuts at: 830 3345 4621 4718 830 Size: 2515 1276 97 3478 Fragments arranged by size: 3478 2515 1276 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7366 PvuII CAG'CTG Cuts at: 4399 4399 Size: 7366 SacI G_AGCT'C Cuts at: 2194 2194 Size: 7366 SalI G'TCGA_C Cuts at: 1936 4708 1936 Size: 2772 4594 SbfI CC_TGCA'GG Cuts at: 4718 4718 Size: 7366 ScaI AGT'ACT Cuts at: 2238 5614 7326 2238 Size: 3376 1712 2278 Fragments arranged by size: 3376 2278 1712 SexAI A'CCwGG_T Cuts at: 2625 3613 4245 2625 Size: 988 632 5746 Fragments arranged by size: 5746 988 632 SfcI C'TryA_G Cuts at: 826 1045 2560 2994 3341 4584 4617 4714 5849 Size: 219 1515 434 347 1243 33 97 1135 Cuts at: 5849 6530 6721 826 Size: 681 191 1471 Fragments arranged by size: 1515 1471 1243 1135 681 434 347 219 191 97 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7366 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7366 SmaI CCC'GGG Cuts at: 1872 4693 1872 Size: 2821 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2193 2816 3123 3279 4293 5470 Size: 197 926 250 623 307 156 1014 1177 Cuts at: 5470 6371 6633 820 Size: 901 262 1553 Fragments arranged by size: 1553 1177 1014 926 901 623 307 262 250 197 156 SnaBI TAC'GTA Cuts at: 493 5157 493 Size: 4664 2702 SpeI A'CTAG_T Cuts at: 152 2701 152 Size: 2549 4817 SphI G_CATG'C Cuts at: 1132 4724 1132 Size: 3592 3774 SspI AAT'ATT Cuts at: 5 52 2725 3872 3938 5290 5 Size: 47 2673 1147 66 1352 2081 Fragments arranged by size: 2673 2081 1352 1147 66 47 StuI AGG'CCT Cuts at: 1517 4633 1517 Size: 3116 4250 StyI C'CwwG_G Cuts at: 513 1065 2337 4153 4512 4651 4805 513 Size: 552 1272 1816 359 139 154 3074 Fragments arranged by size: 3074 1816 1272 552 359 154 139 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2990 3302 4211 4642 5393 5410 5563 Size: 1255 1410 312 909 431 751 17 153 Cuts at: 5563 5748 6193 325 Size: 185 445 1498 Fragments arranged by size: 1498 1410 1255 909 751 445 431 312 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2236 4015 4532 Size: 274 80 33 51 1443 259 1779 517 Cuts at: 4532 5612 7324 96 Size: 1080 1712 138 Fragments arranged by size: 1779 1712 1443 1080 517 274 259 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2965 2997 3964 3996 7326 7358 2965 Size: 32 967 32 3330 32 2973 Fragments arranged by size: 3330 2973 967 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3311 1316 Size: 1995 5371 XbaI T'CTAG_A Cuts at: 2212 2303 4702 2212 Size: 91 2399 4876 Fragments arranged by size: 4876 2399 91 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2474 2474 Size: 7366 XhoI C'TCGA_G Cuts at: 1943 3123 1943 Size: 1180 6186 XmaI C'CCGG_G Cuts at: 1870 4691 1870 Size: 2821 4545 XmnI GAAnn'nnTTC Cuts at: 5495 5495 Size: 7366 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5174 276 Size: 53 83 186 936 3640 2468 Fragments arranged by size: 3640 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI