Restriction map for
pFN21AE4055
(Linear) (Six-base) MAPSORT of: KIEE4055.seq Check: 9322 from: 1 to: 2661
KIEE4055.orf
With 182 enzymes: SgfI *
July 3, 2012 18:27 ..
AccI GT'mk_AC
Cuts at: 0 31 314 1376 2661
Size: 31 283 1062 1285
Fragments arranged by size:
1285 1062 283 31
Acc65I G'GTAC_C
Cuts at: 0 265 2661
Size: 265 2396
AclI AA'CG_TT
Cuts at: 0 1109 1235 2661
Size: 1109 126 1426
Fragments arranged by size:
1426 1109 126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 911 979 1147 2200 2443 2651 2661
Size: 376 535 68 168 1053 243 208 10
Fragments arranged by size:
1053 535 376 243 208 168 68 10
AflII C'TTAA_G
Cuts at: 0 811 2661
Size: 811 1850
AleI CACnn'nnGTG
Cuts at: 0 960 2661
Size: 960 1701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2135 2661
Size: 2135 526
AlwNI CAG_nnn'CTG
Cuts at: 0 889 2661
Size: 889 1772
ApoI r'AATT_y
Cuts at: 0 55 827 1423 1576 2661
Size: 55 772 596 153 1085
Fragments arranged by size:
1085 772 596 153 55
AvaI C'yCGr_G
Cuts at: 0 1118 2661
Size: 1118 1543
BanI G'GyrC_C
Cuts at: 0 265 2661
Size: 265 2396
BanII G_rGCy'C
Cuts at: 0 189 859 2661
Size: 189 670 1802
Fragments arranged by size:
1802 670 189
BbsI GAAGACnn'nnnn_
Cuts at: 0 1666 2288 2347 2661
Size: 1666 622 59 314
Fragments arranged by size:
1666 622 314 59
BciVI GTATCCnnnnn_n'
Cuts at: 0 133 2661
Size: 133 2528
BclI T'GATC_A
Cuts at: 0 60 1407 1716 1947 2661
Size: 60 1347 309 231 714
Fragments arranged by size:
1347 714 309 231 60
BglII A'GATC_T
Cuts at: 0 1821 2661
Size: 1821 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1649 2661
Size: 1649 1012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 1259 2273 2661
Size: 173 1086 1014 388
Fragments arranged by size:
1086 1014 388 173
BsaAI yAC'GTr
Cuts at: 0 1380 2661
Size: 1380 1281
BsaBI GATnn'nnATC
Cuts at: 0 1541 1715 2661
Size: 1541 174 946
Fragments arranged by size:
1541 946 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 846 876 1019 1049 2661
Size: 846 30 143 30 1612
Fragments arranged by size:
1612 846 143 30 30
BseYI C'CCAG_C
Cuts at: 0 2300 2661
Size: 2300 361
BsiEI CG_ry'CG
Cuts at: 0 1283 2661
Size: 1283 1378
BsiHKAI G_wGCw'C
Cuts at: 0 189 1758 2661
Size: 189 1569 903
Fragments arranged by size:
1569 903 189
BsmI GAATG_Cn'
Cuts at: 0 1553 1799 2661
Size: 1553 246 862
Fragments arranged by size:
1553 862 246
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2476 2661
Size: 2476 185
Bsp1286I G_dGCh'C
Cuts at: 0 189 859 1758 2661
Size: 189 670 899 903
Fragments arranged by size:
903 899 670 189
BspHI T'CATG_A
Cuts at: 0 836 2661
Size: 836 1825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2103 2661
Size: 2103 558
BsrFI r'CCGG_y
Cuts at: 0 2033 2661
Size: 2033 628
BsrGI T'GTAC_A
Cuts at: 0 2010 2661
Size: 2010 651
BstAPI GCAn_nnn'nTGC
Cuts at: 0 643 2661
Size: 643 2018
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2196 2661
Size: 2196 465
BstYI r'GATC_y
Cuts at: 0 149 216 225 1083 1821 1912 2661
Size: 149 67 9 858 738 91 749
Fragments arranged by size:
858 749 738 149 91 67 9
Bsu36I CC'TnA_GG
Cuts at: 0 145 2661
Size: 145 2516
BtgI C'CryG_G
Cuts at: 0 2507 2661
Size: 2507 154
BtsI GCAGTG_nn'
Cuts at: 0 1069 1333 2141 2609 2661
Size: 1069 264 808 468 52
Fragments arranged by size:
1069 808 468 264 52
DraI TTT'AAA
Cuts at: 0 12 1022 2661
Size: 12 1010 1639
Fragments arranged by size:
1639 1010 12
DraIII CAC_nnn'GTG
Cuts at: 0 1198 2661
Size: 1198 1463
DrdI GACnn_nn'nnGTC
Cuts at: 0 2349 2661
Size: 2349 312
EaeI y'GGCC_r
Cuts at: 0 2466 2661
Size: 2466 195
EarI CTCTTCn'nnn_
Cuts at: 0 594 1690 2078 2176 2350 2661
Size: 594 1096 388 98 174 311
Fragments arranged by size:
1096 594 388 311 174 98
EcoICRI GAG'CTC
Cuts at: 0 187 2661
Size: 187 2474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 911 979 1147 1649 2200 2443 2651
Size: 376 535 68 168 502 551 243 208
Cuts at: 2651 2661
Size: 10
Fragments arranged by size:
551 535 502 376 243 208 168 68
10
EcoRV GAT'ATC
Cuts at: 0 1795 2661
Size: 1795 866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 220 2661
Size: 220 2441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 903 935 1019 1023 1051 1055
Size: 13 32 858 32 84 4 28 4
Cuts at: 1055 1392 1424 1935 1967 2440 2472 2661
Size: 337 32 511 32 473 32 189
Fragments arranged by size:
858 511 473 337 189 84 32 32
32 32 32 28 13 4 4
HincII GTy'rAC
Cuts at: 0 90 1185 2661
Size: 90 1095 1476
Fragments arranged by size:
1476 1095 90
HindIII A'AGCT_T
Cuts at: 0 844 1455 2227 2661
Size: 844 611 772 434
Fragments arranged by size:
844 772 611 434
HpaI GTT'AAC
Cuts at: 0 1185 2661
Size: 1185 1476
KpnI G_GTAC'C
Cuts at: 0 269 2661
Size: 269 2392
MslI CAynn'nnrTG
Cuts at: 0 960 2512 2661
Size: 960 1552 149
Fragments arranged by size:
1552 960 149
MspA1I CmG'CkG
Cuts at: 0 2394 2661
Size: 2394 267
NaeI GCC'GGC
Cuts at: 0 2035 2661
Size: 2035 626
NcoI C'CATG_G
Cuts at: 0 2507 2661
Size: 2507 154
NdeI CA'TA_TG
Cuts at: 0 742 2661
Size: 742 1919
NgoMIV G'CCGG_C
Cuts at: 0 2033 2661
Size: 2033 628
NspI r_CATG'y
Cuts at: 0 933 1901 2661
Size: 933 968 760
Fragments arranged by size:
968 933 760
PflMI CCAn_nnn'nTGG
Cuts at: 0 468 603 2661
Size: 468 135 2058
Fragments arranged by size:
2058 468 135
PshAI GACnn'nnGTC
Cuts at: 0 2509 2661
Size: 2509 152
PsiI TTA'TAA
Cuts at: 0 614 2661
Size: 614 2047
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 888 920 2661
Size: 888 32 1741
Fragments arranged by size:
1741 888 32
PstI C_TGCA'G
Cuts at: 0 1340 2616 2661
Size: 1340 1276 45
Fragments arranged by size:
1340 1276 45
PvuII CAG'CTG
Cuts at: 0 2394 2661
Size: 2394 267
SacI G_AGCT'C
Cuts at: 0 189 2661
Size: 189 2472
ScaI AGT'ACT
Cuts at: 0 233 2661
Size: 233 2428
SexAI A'CCwGG_T
Cuts at: 0 620 1608 2240 2661
Size: 620 988 632 421
Fragments arranged by size:
988 632 620 421
SfcI C'TryA_G
Cuts at: 0 555 989 1336 2579 2612 2661
Size: 555 434 347 1243 33 49
Fragments arranged by size:
1243 555 434 347 49 33
SmlI C'TyrA_G
Cuts at: 0 188 811 1118 1274 2288 2661
Size: 188 623 307 156 1014 373
Fragments arranged by size:
1014 623 373 307 188 156
SpeI A'CTAG_T
Cuts at: 0 696 2661
Size: 696 1965
SspI AAT'ATT
Cuts at: 0 720 1867 1933 2661
Size: 720 1147 66 728
Fragments arranged by size:
1147 728 720 66
StuI AGG'CCT
Cuts at: 0 2628 2661
Size: 2628 33
StyI C'CwwG_G
Cuts at: 0 332 2148 2507 2646 2661
Size: 332 1816 359 139 15
Fragments arranged by size:
1816 359 332 139 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 985 1297 2206 2637 2661
Size: 985 312 909 431 24
Fragments arranged by size:
985 909 431 312 24
TatI w'GTAC_w
Cuts at: 0 231 2010 2527 2661
Size: 231 1779 517 134
Fragments arranged by size:
1779 517 231 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 960 992 1959 1991 2661
Size: 960 32 967 32 670
Fragments arranged by size:
967 960 670 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1306 2661
Size: 1306 1355
XbaI T'CTAG_A
Cuts at: 0 207 298 2661
Size: 207 91 2363
Fragments arranged by size:
2363 207 91
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 469 2661
Size: 469 2192
XhoI C'TCGA_G
Cuts at: 0 1118 2661
Size: 1118 1543
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AleI AlfI AlwNI
ApoI AvaI BanI BanII BbsI BciVI BclI BglII
BpmI BpuEI BsaAI BsaBI BsaXI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoRV FalI Hin4I HincII
HindIII HpaI KpnI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PflMI PshAI PsiI PsrI PstI PvuII
SacI ScaI SexAI SfcI SmlI SpeI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII AfeI AflIII AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaHI BsaWI BseRI BsgI
BsiWI BspEI BsrBI BsrDI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI CspCI EagI EciI EcoNI EcoO109I
EcoRI FseI FspI FspAI HaeII KasI MfeI MluI
MmeI MscI NarI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SwaI XmaI XmnI ZraI