(Circular) (Six-base) MAPSORT of: pFN21AE3353.seq Check: 2709 from: 1 to: 7837 pFN21AE3353 7837 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2225 2719 3844 4203 2225 Size: 494 1125 359 5859 Fragments arranged by size: 5859 1125 494 359 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4401 5647 278 Size: 53 83 186 936 2865 1246 2468 Fragments arranged by size: 2865 2468 1246 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2094 5180 1937 Size: 157 3086 4594 Fragments arranged by size: 4594 3086 157 Acc65I G'GTAC_C Cuts at: 2546 5158 2546 Size: 2612 5225 AclI AA'CG_TT Cuts at: 1563 2583 5964 6337 1563 Size: 1020 3381 373 3063 Fragments arranged by size: 3381 3063 1020 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3742 4100 4251 4601 5060 5900 7284 7552 Size: 2110 358 151 350 459 840 1384 268 Cuts at: 7552 1632 Size: 1917 Fragments arranged by size: 2110 1917 1384 840 459 358 350 268 151 AflII C'TTAA_G Cuts at: 820 1017 3299 820 Size: 197 2282 5358 Fragments arranged by size: 5358 2282 197 AflIII A'CryG_T Cuts at: 1203 3324 5115 6736 7713 1203 Size: 2121 1791 1621 977 1327 Fragments arranged by size: 2121 1791 1621 1327 977 AgeI A'CCGG_T Cuts at: 6668 6668 Size: 7837 AhdI GACnn_n'nnGTC Cuts at: 6566 6566 Size: 7837 AleI CACnn'nnGTG Cuts at: 1166 4664 1166 Size: 3498 4339 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2816 3077 1184 Size: 1632 261 5944 Fragments arranged by size: 5944 1632 261 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2954 2986 2954 Size: 32 7805 AlwNI CAG_nnn'CTG Cuts at: 2149 2473 2698 3127 3838 5580 7152 2149 Size: 324 225 429 711 1742 1572 2834 Fragments arranged by size: 2834 1742 1572 711 429 324 225 ApaI G_GGCC'C Cuts at: 2572 3916 2572 Size: 1344 6493 ApaLI G'TGCA_C Cuts at: 4249 5894 7050 4249 Size: 1645 1156 5036 Fragments arranged by size: 5036 1645 1156 ApoI r'AATT_y Cuts at: 1493 2033 3896 4756 4859 5146 5372 5524 7633 Size: 540 1863 860 103 287 226 152 2109 Cuts at: 7633 1493 Size: 1697 Fragments arranged by size: 2109 1863 1697 860 540 287 226 152 103 AseI AT'TA_AT Cuts at: 160 1032 6391 160 Size: 872 5359 1606 Fragments arranged by size: 5359 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7837 AvaI C'yCGr_G Cuts at: 1870 1943 2198 3431 4594 5162 1870 Size: 73 255 1233 1163 568 4545 Fragments arranged by size: 4545 1233 1163 568 255 73 BamHI G'GATC_C Cuts at: 5167 5534 5167 Size: 367 7470 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2154 2546 3951 4908 5158 Size: 325 217 627 367 392 1405 957 250 Cuts at: 5158 6613 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1405 957 627 392 367 325 250 217 BanII G_rGCy'C Cuts at: 1391 2523 2572 2985 3111 3916 4112 4971 5156 Size: 1132 49 413 126 805 196 859 185 Cuts at: 5156 1391 Size: 4072 Fragments arranged by size: 4072 1132 859 805 413 196 185 126 49 BbeI G_GCGC'C Cuts at: 4912 4912 Size: 7837 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2721 4124 4847 928 Size: 971 822 1403 723 3918 Fragments arranged by size: 3918 1403 971 822 723 BbvCI CC'TCA_GC Cuts at: 2129 2129 Size: 7837 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6028 6062 1903 Size: 34 50 34 4007 34 3678 Fragments arranged by size: 4007 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5729 6939 5729 Size: 1210 6627 BclI T'GATC_A Cuts at: 1548 1548 Size: 7837 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3212 3780 6448 Size: 107 122 71 631 605 1540 568 2668 Cuts at: 6448 136 Size: 1525 Fragments arranged by size: 2668 1540 1525 631 605 568 122 107 71 BglII A'GATC_T Cuts at: 3474 7832 3474 Size: 4358 3479 BlpI GC'TnA_GC Cuts at: 4106 4970 5254 4106 Size: 864 284 6689 Fragments arranged by size: 6689 864 284 Bme1580I G_kGCm'C Cuts at: 1790 2572 2665 3377 3916 4253 5898 7054 1790 Size: 782 93 712 539 337 1645 1156 2573 Fragments arranged by size: 2573 1645 1156 782 712 539 337 93 BmgBI CAC'GTC Cuts at: 1318 2278 2638 1318 Size: 960 360 6517 Fragments arranged by size: 6517 960 360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 5024 5568 Size: 416 53 83 399 24 741 2866 544 Cuts at: 5568 6526 442 Size: 958 1753 Fragments arranged by size: 2866 1753 958 741 544 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3644 1056 Size: 2588 5249 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2407 4204 2407 Size: 1797 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2537 2981 3149 3488 3569 3764 4028 Size: 1031 267 444 168 339 81 195 264 Cuts at: 4028 6497 1239 Size: 2469 2579 Fragments arranged by size: 2579 2469 1031 444 339 267 264 195 168 81 Bpu10I CC'TnA_GC Cuts at: 2129 3734 4965 2129 Size: 1605 1231 5001 Fragments arranged by size: 5001 1605 1231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3845 4982 5962 6827 7125 3845 Size: 1137 980 865 298 4557 Fragments arranged by size: 4557 1137 980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6500 882 Size: 617 5001 2219 Fragments arranged by size: 5001 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3778 3973 5628 493 Size: 711 2574 195 1655 2702 Fragments arranged by size: 2702 2574 1655 711 195 BsaBI GATnn'nnATC Cuts at: 7831 7831 Size: 7837 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2266 4398 4909 5644 Size: 53 83 186 936 733 2132 511 735 Cuts at: 5644 6026 6661 275 Size: 382 635 1451 Fragments arranged by size: 2132 1451 936 735 733 635 511 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6270 6668 6942 7089 7500 6270 Size: 398 274 147 411 6607 Fragments arranged by size: 6607 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2954 2984 3098 3128 3767 Size: 30 165 30 1551 30 114 30 639 Cuts at: 3767 3797 3944 3974 1178 Size: 30 147 30 5041 Fragments arranged by size: 5041 1551 639 165 147 114 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2351 2462 2618 2833 3533 3536 4316 Size: 877 288 111 156 215 700 3 780 Cuts at: 4316 4637 5042 5045 1186 Size: 321 405 3 3978 Fragments arranged by size: 3978 877 780 700 405 321 288 215 156 111 3 3 BseYI C'CCAG_C Cuts at: 2473 2478 3484 4214 4669 7040 7536 2473 Size: 5 1006 730 455 2371 496 2774 Fragments arranged by size: 2774 2371 1006 730 496 455 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 2786 3053 3872 1163 Size: 573 564 486 267 819 5128 Fragments arranged by size: 5128 819 573 564 486 267 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2326 2457 4684 5287 6048 7076 Size: 275 186 325 131 2227 603 761 1028 Cuts at: 7076 1540 Size: 2301 Fragments arranged by size: 2301 2227 1028 761 603 325 275 186 131 BsiHKAI G_wGCw'C Cuts at: 2985 3104 3582 3627 3713 3861 4112 4253 4971 Size: 119 478 45 86 148 251 141 718 Cuts at: 4971 5898 5983 7054 2985 Size: 927 85 1071 3768 Fragments arranged by size: 3768 1071 927 718 478 251 148 141 119 86 85 45 BsmI GAATG_Cn' Cuts at: 5348 5441 5348 Size: 93 7744 BsmBI CGTCTCn'nnnn_ Cuts at: 3176 3176 Size: 7837 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2523 2572 2665 2985 3104 3111 3377 Size: 399 733 49 93 320 119 7 266 Cuts at: 3377 3582 3627 3713 3861 3916 4112 4253 4971 Size: 205 45 86 148 55 196 141 718 Cuts at: 4971 5156 5898 5983 7054 1391 Size: 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 733 718 399 320 266 205 196 185 148 141 119 93 86 85 55 49 45 7 BspEI T'CCGG_A Cuts at: 7500 7500 Size: 7837 BspHI T'CATG_A Cuts at: 4701 5726 7456 4701 Size: 1025 1730 5082 Fragments arranged by size: 5082 1730 1025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2225 2719 3162 3711 3844 3846 4203 5125 Size: 1381 494 443 549 133 2 357 922 Cuts at: 5125 5192 844 Size: 67 3489 Fragments arranged by size: 3489 1381 922 549 494 443 357 133 67 2 BsrBI CCG'CTC Cuts at: 1394 1823 2176 2770 3016 3553 3960 5724 1394 Size: 429 353 594 246 537 407 1764 3507 Fragments arranged by size: 3507 1764 594 537 429 407 353 246 BsrDI GCAATG_nn' Cuts at: 65 1248 2125 3255 6332 6506 65 Size: 1183 877 1130 3077 174 1396 Fragments arranged by size: 3077 1396 1183 1130 877 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2463 6481 6668 1700 Size: 110 653 4018 187 2869 Fragments arranged by size: 4018 2869 653 187 110 BsrGI T'GTAC_A Cuts at: 96 4290 96 Size: 4194 3643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7837 BssSI C'ACGA_G Cuts at: 2381 3581 5897 6909 2381 Size: 1200 2316 1012 3309 Fragments arranged by size: 3309 2316 1200 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3850 4366 794 Size: 3056 516 4265 Fragments arranged by size: 4265 3056 516 BstBI TT'CG_AA Cuts at: 6641 6641 Size: 7837 BstEII G'GTnAC_C Cuts at: 1184 3658 1184 Size: 2474 5363 BstYI r'GATC_y Cuts at: 1972 3474 5167 5534 5919 5936 7388 7474 7486 Size: 1502 1693 367 385 17 1452 86 12 Cuts at: 7486 7832 1972 Size: 346 1977 Fragments arranged by size: 1977 1693 1502 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4427 4427 Size: 7837 BtgI C'CryG_G Cuts at: 513 1065 2028 2829 3049 3369 4912 513 Size: 552 963 801 220 320 1543 3438 Fragments arranged by size: 3438 1543 963 801 552 320 220 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4489 487 Size: 683 56 3263 3835 Fragments arranged by size: 3835 3263 683 56 BtsI GCAGTG_nn' Cuts at: 4253 5353 6147 6167 4253 Size: 1100 794 20 5923 Fragments arranged by size: 5923 1100 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7837 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7802 DraI TTT'AAA Cuts at: 5141 5141 Size: 7837 DraIII CAC_nnn'GTG Cuts at: 3451 3666 5116 3451 Size: 215 1450 6172 Fragments arranged by size: 6172 1450 215 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6844 809 Size: 507 5528 1802 Fragments arranged by size: 5528 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2454 3716 3918 Size: 54 1425 50 275 18 624 1262 202 Cuts at: 3918 3934 4656 4741 5284 6173 7802 8 Size: 16 722 85 543 889 1629 43 Fragments arranged by size: 1629 1425 1262 889 722 624 543 275 202 85 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2454 5284 1537 Size: 275 642 2830 4090 Fragments arranged by size: 4090 2830 642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 4928 5767 1353 Size: 830 2745 839 3423 Fragments arranged by size: 3423 2745 839 830 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3260 6424 6798 6944 387 Size: 1064 138 1671 3164 374 146 1280 Fragments arranged by size: 3164 1671 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2983 4110 4969 2983 Size: 1127 859 5851 Fragments arranged by size: 5851 1127 859 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2537 2981 3149 3488 3569 3742 Size: 393 638 267 444 168 339 81 173 Cuts at: 3742 3764 4028 4100 4251 4601 5060 5900 6497 Size: 22 264 72 151 350 459 840 597 Cuts at: 6497 7284 7552 1239 Size: 787 268 1524 Fragments arranged by size: 1524 840 787 638 597 459 444 393 350 339 268 267 264 173 168 151 81 72 22 EcoNI CCTnn'n_nnAGG Cuts at: 3431 4018 4358 4478 3431 Size: 587 340 120 6790 Fragments arranged by size: 6790 587 340 120 EcoO109I rG'GnC_Cy Cuts at: 2540 2569 3589 3913 4084 4183 4603 4890 2540 Size: 29 1020 324 171 99 420 287 5487 Fragments arranged by size: 5487 1020 420 324 287 171 99 29 EcoRI G'AATT_C Cuts at: 2033 5146 5524 2033 Size: 3113 378 4346 Fragments arranged by size: 4346 3113 378 FspI TGC'GCA Cuts at: 3383 6343 3383 Size: 2960 4877 HaeII r_GCGC'y Cuts at: 1678 2651 3408 3641 3741 4912 6984 1678 Size: 973 757 233 100 1171 2072 2531 Fragments arranged by size: 2531 2072 1171 973 757 233 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 6478 6510 6552 6584 1373 Size: 32 135 32 4906 32 42 32 2626 Fragments arranged by size: 4906 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2071 2581 2814 5181 5427 669 Size: 988 281 133 510 233 2367 246 3079 Fragments arranged by size: 3079 2367 988 510 281 246 233 133 HindIII A'AGCT_T Cuts at: 748 748 Size: 7837 HpaI GTT'AAC Cuts at: 5427 5427 Size: 7837 KasI G'GCGC_C Cuts at: 4908 4908 Size: 7837 KpnI G_GTAC'C Cuts at: 2550 5162 2550 Size: 2612 5225 MfeI C'AATT_G Cuts at: 5436 5436 Size: 7837 MluI A'CGCG_T Cuts at: 7713 7713 Size: 7837 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4540 6951 7135 1305 Size: 3235 2411 184 2007 Fragments arranged by size: 3235 2411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3920 4658 4743 10 Size: 54 1425 343 2088 738 85 3104 Fragments arranged by size: 3104 2088 1425 738 343 85 54 MslI CAynn'nnrTG Cuts at: 518 1166 3681 4043 4256 4481 4664 5795 6154 Size: 648 2515 362 213 225 183 1131 359 Cuts at: 6154 6313 518 Size: 159 2042 Fragments arranged by size: 2515 2042 1131 648 362 359 225 213 183 159 MspA1I CmG'CkG Cuts at: 1606 2146 2234 2473 2515 2644 2698 2796 3124 Size: 540 88 239 42 129 54 98 328 Cuts at: 3124 3410 3529 3535 3847 4314 4723 4974 5253 Size: 286 119 6 312 467 409 251 279 Cuts at: 5253 5930 7078 7323 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 540 467 409 328 312 286 279 251 245 239 129 119 98 88 54 42 6 NaeI GCC'GGC Cuts at: 2465 2465 Size: 7837 NarI GG'CG_CC Cuts at: 4909 4909 Size: 7837 NcoI C'CATG_G Cuts at: 513 1065 2028 2829 513 Size: 552 963 801 5521 Fragments arranged by size: 5521 963 801 552 NdeI CA'TA_TG Cuts at: 387 4663 387 Size: 4276 3561 NgoMIV G'CCGG_C Cuts at: 2463 2463 Size: 7837 NheI G'CTAG_C Cuts at: 1052 3640 1052 Size: 2588 5249 NotI GC'GGCC_GC Cuts at: 2454 5284 2454 Size: 2830 5007 NspI r_CATG'y Cuts at: 1132 1229 2188 3328 3512 4162 4369 5195 6740 Size: 97 959 1140 184 650 207 826 1545 Cuts at: 6740 1132 Size: 2229 Fragments arranged by size: 2229 1545 1140 959 826 650 207 184 97 PasI CC'CwG_GG Cuts at: 1348 2012 1348 Size: 664 7173 PciI A'CATG_T Cuts at: 3324 6736 3324 Size: 3412 4425 PflMI CCAn_nnn'nTGG Cuts at: 1108 3261 4393 4750 1108 Size: 2153 1132 357 4195 Fragments arranged by size: 4195 2153 1132 357 PfoI T'CCnGG_A Cuts at: 3546 3618 3630 4005 3546 Size: 72 12 375 7378 Fragments arranged by size: 7378 375 72 12 PmeI GTTT'AAAC Cuts at: 5141 5141 Size: 7837 PmlI CAC'GTG Cuts at: 3973 3973 Size: 7837 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2954 2986 3303 3335 5906 5938 7419 7451 2954 Size: 32 317 32 2571 32 1481 32 3340 Fragments arranged by size: 3340 2571 1481 317 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3589 3589 Size: 7837 PshAI GACnn'nnGTC Cuts at: 1613 2831 1613 Size: 1218 6619 PsiI TTA'TAA Cuts at: 5407 5407 Size: 7837 PspOMI G'GGCC_C Cuts at: 2568 3912 2568 Size: 1344 6493 PstI C_TGCA'G Cuts at: 830 2496 2514 2730 2795 3534 3846 4022 4200 Size: 1666 18 216 65 739 312 176 178 Cuts at: 4200 4380 5189 830 Size: 180 809 3478 Fragments arranged by size: 3478 1666 809 739 312 216 180 178 176 65 18 PvuI CG_AT'CG Cuts at: 2001 4684 2001 Size: 2683 5154 PvuII CAG'CTG Cuts at: 2146 2473 2515 2796 3529 3535 3847 4723 4974 Size: 327 42 281 733 6 312 876 251 Cuts at: 4974 2146 Size: 5009 Fragments arranged by size: 5009 876 733 327 312 281 251 42 6 SacI G_AGCT'C Cuts at: 2985 4112 4971 2985 Size: 1127 859 5851 Fragments arranged by size: 5851 1127 859 SalI G'TCGA_C Cuts at: 1936 5179 1936 Size: 3243 4594 SapI GCTCTTCn'nnn_ Cuts at: 2183 2183 Size: 7837 SbfI CC_TGCA'GG Cuts at: 5189 5189 Size: 7837 ScaI AGT'ACT Cuts at: 6085 7797 6085 Size: 1712 6125 SexAI A'CCwGG_T Cuts at: 3591 3591 Size: 7837 SfcI C'TryA_G Cuts at: 826 1045 2492 2510 2726 2791 3530 3842 4018 Size: 219 1447 18 216 65 739 312 176 Cuts at: 4018 4196 4376 5185 6320 7001 7192 826 Size: 178 180 809 1135 681 191 1471 Fragments arranged by size: 1471 1447 1135 809 739 681 312 219 216 191 180 178 176 65 18 SfoI GGC'GCC Cuts at: 4910 4910 Size: 7837 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7837 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7837 SmaI CCC'GGG Cuts at: 1872 4596 5164 1872 Size: 2724 568 4545 Fragments arranged by size: 4545 2724 568 SmlI C'TyrA_G Cuts at: 820 1017 1943 3299 3860 4997 5941 6842 7104 Size: 197 926 1356 561 1137 944 901 262 Cuts at: 7104 820 Size: 1553 Fragments arranged by size: 1553 1356 1137 944 926 901 561 262 197 SnaBI TAC'GTA Cuts at: 493 5628 493 Size: 5135 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7837 SphI G_CATG'C Cuts at: 1132 4162 5195 1132 Size: 3030 1033 3774 Fragments arranged by size: 3774 3030 1033 SspI AAT'ATT Cuts at: 5 52 5761 5 Size: 47 5709 2081 Fragments arranged by size: 5709 2081 47 StuI AGG'CCT Cuts at: 1517 2204 2376 2486 4013 4172 4473 4959 1517 Size: 687 172 110 1527 159 301 486 4395 Fragments arranged by size: 4395 1527 687 486 301 172 159 110 StyI C'CwwG_G Cuts at: 513 1065 2028 2043 2631 2829 4137 5276 513 Size: 552 963 15 588 198 1308 1139 3074 Fragments arranged by size: 3074 1308 1139 963 588 552 198 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4046 5864 5881 6034 6219 6664 325 Size: 1255 2466 1818 17 153 185 445 1498 Fragments arranged by size: 2466 1818 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 2784 3453 Size: 274 80 33 51 1443 519 288 669 Cuts at: 3453 4290 6083 7795 96 Size: 837 1793 1712 138 Fragments arranged by size: 1793 1712 1443 837 669 519 288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3098 3130 3581 3613 3767 3799 3944 3976 4184 Size: 32 451 32 154 32 145 32 208 Cuts at: 4184 4216 4388 4420 5102 5134 7797 7829 3098 Size: 32 172 32 682 32 2663 32 3106 Fragments arranged by size: 3106 2663 682 451 208 172 154 145 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3076 4097 4817 1316 Size: 1760 1021 720 4336 Fragments arranged by size: 4336 1760 1021 720 XbaI T'CTAG_A Cuts at: 5173 5173 Size: 7837 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2189 3901 4222 4666 4736 2189 Size: 1712 321 444 70 5290 Fragments arranged by size: 5290 1712 444 321 70 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7837 XmaI C'CCGG_G Cuts at: 1870 4594 5162 1870 Size: 2724 568 4545 Fragments arranged by size: 4545 2724 568 XmnI GAAnn'nnTTC Cuts at: 4094 5045 5966 4094 Size: 951 921 5965 Fragments arranged by size: 5965 951 921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4399 5645 276 Size: 53 83 186 936 2865 1246 2468 Fragments arranged by size: 2865 2468 1246 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI