Restriction map for pFN21AE3353
 (Circular) (Six-base) MAPSORT of: pFN21AE3353.seq  Check: 2709  from: 1  to: 7837



pFN21AE3353  7837 bp

 With 182 enzymes: SgfI * 

                        July 6, 2012 17:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2225    2719    3844    4203    2225 
   Size:        494    1125     359    5859

  Fragments arranged by size: 

                5859    1125     494     359
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4401    5647     278 
   Size:         53      83     186     936    2865    1246    2468

  Fragments arranged by size: 

                2865    2468    1246     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2094    5180    1937 
   Size:        157    3086    4594

  Fragments arranged by size: 

                4594    3086     157
 
Acc65I G'GTAC_C

Cuts at:   2546    5158    2546 
   Size:       2612    5225
 
AclI AA'CG_TT

Cuts at:   1563    2583    5964    6337    1563 
   Size:       1020    3381     373    3063

  Fragments arranged by size: 

                3381    3063    1020     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3742    4100    4251    4601    5060    5900    7284    7552 
   Size:       2110     358     151     350     459     840    1384     268

Cuts at:   7552    1632 
   Size:       1917

  Fragments arranged by size: 

                2110    1917    1384     840     459     358     350     268
                 151
 
AflII C'TTAA_G

Cuts at:    820    1017    3299     820 
   Size:        197    2282    5358

  Fragments arranged by size: 

                5358    2282     197
 
AflIII A'CryG_T

Cuts at:   1203    3324    5115    6736    7713    1203 
   Size:       2121    1791    1621     977    1327

  Fragments arranged by size: 

                2121    1791    1621    1327     977
 
AgeI A'CCGG_T

Cuts at:   6668    6668 
   Size:       7837
 
AhdI GACnn_n'nnGTC

Cuts at:   6566    6566 
   Size:       7837
 
AleI CACnn'nnGTG

Cuts at:   1166    4664    1166 
   Size:       3498    4339
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2816    3077    1184 
   Size:       1632     261    5944

  Fragments arranged by size: 

                5944    1632     261
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2954    2986    2954 
   Size:         32    7805
 
AlwNI CAG_nnn'CTG

Cuts at:   2149    2473    2698    3127    3838    5580    7152    2149 
   Size:        324     225     429     711    1742    1572    2834

  Fragments arranged by size: 

                2834    1742    1572     711     429     324     225
 
ApaI G_GGCC'C

Cuts at:   2572    3916    2572 
   Size:       1344    6493
 
ApaLI G'TGCA_C

Cuts at:   4249    5894    7050    4249 
   Size:       1645    1156    5036

  Fragments arranged by size: 

                5036    1645    1156
 
ApoI r'AATT_y

Cuts at:   1493    2033    3896    4756    4859    5146    5372    5524    7633 
   Size:        540    1863     860     103     287     226     152    2109

Cuts at:   7633    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1863    1697     860     540     287     226     152
                 103
 
AseI AT'TA_AT

Cuts at:    160    1032    6391     160 
   Size:        872    5359    1606

  Fragments arranged by size: 

                5359    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7837
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2198    3431    4594    5162    1870 
   Size:         73     255    1233    1163     568    4545

  Fragments arranged by size: 

                4545    1233    1163     568     255      73
 
BamHI G'GATC_C

Cuts at:   5167    5534    5167 
   Size:        367    7470
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2154    2546    3951    4908    5158 
   Size:        325     217     627     367     392    1405     957     250

Cuts at:   5158    6613     618 
   Size:       1455    1842

  Fragments arranged by size: 

                1842    1455    1405     957     627     392     367     325
                 250     217
 
BanII G_rGCy'C

Cuts at:   1391    2523    2572    2985    3111    3916    4112    4971    5156 
   Size:       1132      49     413     126     805     196     859     185

Cuts at:   5156    1391 
   Size:       4072

  Fragments arranged by size: 

                4072    1132     859     805     413     196     185     126
                  49
 
BbeI G_GCGC'C

Cuts at:   4912    4912 
   Size:       7837
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2721    4124    4847     928 
   Size:        971     822    1403     723    3918

  Fragments arranged by size: 

                3918    1403     971     822     723
 
BbvCI CC'TCA_GC

Cuts at:   2129    2129 
   Size:       7837
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    6028    6062    1903 
   Size:         34      50      34    4007      34    3678

  Fragments arranged by size: 

                4007    3678      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   5729    6939    5729 
   Size:       1210    6627
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       7837
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3212    3780    6448 
   Size:        107     122      71     631     605    1540     568    2668

Cuts at:   6448     136 
   Size:       1525

  Fragments arranged by size: 

                2668    1540    1525     631     605     568     122     107
                  71
 
BglII A'GATC_T

Cuts at:   3474    7832    3474 
   Size:       4358    3479
 
BlpI GC'TnA_GC

Cuts at:   4106    4970    5254    4106 
   Size:        864     284    6689

  Fragments arranged by size: 

                6689     864     284
 
Bme1580I G_kGCm'C

Cuts at:   1790    2572    2665    3377    3916    4253    5898    7054    1790 
   Size:        782      93     712     539     337    1645    1156    2573

  Fragments arranged by size: 

                2573    1645    1156     782     712     539     337      93
            
 
BmgBI CAC'GTC

Cuts at:   1318    2278    2638    1318 
   Size:        960     360    6517

  Fragments arranged by size: 

                6517     960     360
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2158    5024    5568 
   Size:        416      53      83     399      24     741    2866     544

Cuts at:   5568    6526     442 
   Size:        958    1753

  Fragments arranged by size: 

                2866    1753     958     741     544     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    3644    1056 
   Size:       2588    5249
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2407    4204    2407 
   Size:       1797    6040
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2270    2537    2981    3149    3488    3569    3764    4028 
   Size:       1031     267     444     168     339      81     195     264

Cuts at:   4028    6497    1239 
   Size:       2469    2579

  Fragments arranged by size: 

                2579    2469    1031     444     339     267     264     195
                 168      81
 
Bpu10I CC'TnA_GC

Cuts at:   2129    3734    4965    2129 
   Size:       1605    1231    5001

  Fragments arranged by size: 

                5001    1605    1231
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3845    4982    5962    6827    7125    3845 
   Size:       1137     980     865     298    4557

  Fragments arranged by size: 

                4557    1137     980     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    6500     882 
   Size:        617    5001    2219

  Fragments arranged by size: 

                5001    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3778    3973    5628     493 
   Size:        711    2574     195    1655    2702

  Fragments arranged by size: 

                2702    2574    1655     711     195
 
BsaBI GATnn'nnATC

Cuts at:   7831    7831 
   Size:       7837
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2266    4398    4909    5644 
   Size:         53      83     186     936     733    2132     511     735

Cuts at:   5644    6026    6661     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                2132    1451     936     735     733     635     511     382
                 186      83      53
 
BsaWI w'CCGG_w

Cuts at:   6270    6668    6942    7089    7500    6270 
   Size:        398     274     147     411    6607

  Fragments arranged by size: 

                6607     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2954    2984    3098    3128    3767 
   Size:         30     165      30    1551      30     114      30     639

Cuts at:   3767    3797    3944    3974    1178 
   Size:         30     147      30    5041

  Fragments arranged by size: 

                5041    1551     639     165     147     114      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2063    2351    2462    2618    2833    3533    3536    4316 
   Size:        877     288     111     156     215     700       3     780

Cuts at:   4316    4637    5042    5045    1186 
   Size:        321     405       3    3978

  Fragments arranged by size: 

                3978     877     780     700     405     321     288     215
                 156     111       3       3
 
BseYI C'CCAG_C

Cuts at:   2473    2478    3484    4214    4669    7040    7536    2473 
   Size:          5    1006     730     455    2371     496    2774

  Fragments arranged by size: 

                2774    2371    1006     730     496     455       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2300    2786    3053    3872    1163 
   Size:        573     564     486     267     819    5128

  Fragments arranged by size: 

                5128     819     573     564     486     267
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2326    2457    4684    5287    6048    7076 
   Size:        275     186     325     131    2227     603     761    1028

Cuts at:   7076    1540 
   Size:       2301

  Fragments arranged by size: 

                2301    2227    1028     761     603     325     275     186
                 131
 
BsiHKAI G_wGCw'C

Cuts at:   2985    3104    3582    3627    3713    3861    4112    4253    4971 
   Size:        119     478      45      86     148     251     141     718

Cuts at:   4971    5898    5983    7054    2985 
   Size:        927      85    1071    3768

  Fragments arranged by size: 

                3768    1071     927     718     478     251     148     141
                 119      86      85      45
 
BsmI GAATG_Cn'

Cuts at:   5348    5441    5348 
   Size:         93    7744
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3176    3176 
   Size:       7837
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2523    2572    2665    2985    3104    3111    3377 
   Size:        399     733      49      93     320     119       7     266

Cuts at:   3377    3582    3627    3713    3861    3916    4112    4253    4971 
   Size:        205      45      86     148      55     196     141     718

Cuts at:   4971    5156    5898    5983    7054    1391 
   Size:        185     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     733     718     399     320     266
                 205     196     185     148     141     119      93      86
                  85      55      49      45       7
 
BspEI T'CCGG_A

Cuts at:   7500    7500 
   Size:       7837
 
BspHI T'CATG_A

Cuts at:   4701    5726    7456    4701 
   Size:       1025    1730    5082

  Fragments arranged by size: 

                5082    1730    1025
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2225    2719    3162    3711    3844    3846    4203    5125 
   Size:       1381     494     443     549     133       2     357     922

Cuts at:   5125    5192     844 
   Size:         67    3489

  Fragments arranged by size: 

                3489    1381     922     549     494     443     357     133
                  67       2
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2176    2770    3016    3553    3960    5724    1394 
   Size:        429     353     594     246     537     407    1764    3507

  Fragments arranged by size: 

                3507    1764     594     537     429     407     353     246
            
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2125    3255    6332    6506      65 
   Size:       1183     877    1130    3077     174    1396

  Fragments arranged by size: 

                3077    1396    1183    1130     877     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2463    6481    6668    1700 
   Size:        110     653    4018     187    2869

  Fragments arranged by size: 

                4018    2869     653     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    4290      96 
   Size:       4194    3643
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7837
 
BssSI C'ACGA_G

Cuts at:   2381    3581    5897    6909    2381 
   Size:       1200    2316    1012    3309

  Fragments arranged by size: 

                3309    2316    1200    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3850    4366     794 
   Size:       3056     516    4265

  Fragments arranged by size: 

                4265    3056     516
 
BstBI TT'CG_AA

Cuts at:   6641    6641 
   Size:       7837
 
BstEII G'GTnAC_C

Cuts at:   1184    3658    1184 
   Size:       2474    5363
 
BstYI r'GATC_y

Cuts at:   1972    3474    5167    5534    5919    5936    7388    7474    7486 
   Size:       1502    1693     367     385      17    1452      86      12

Cuts at:   7486    7832    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1693    1502    1452     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   4427    4427 
   Size:       7837
 
BtgI C'CryG_G

Cuts at:    513    1065    2028    2829    3049    3369    4912     513 
   Size:        552     963     801     220     320    1543    3438

  Fragments arranged by size: 

                3438    1543     963     801     552     320     220
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    4489     487 
   Size:        683      56    3263    3835

  Fragments arranged by size: 

                3835    3263     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4253    5353    6147    6167    4253 
   Size:       1100     794      20    5923

  Fragments arranged by size: 

                5923    1100     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7837
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7802
 
DraI TTT'AAA

Cuts at:   5141    5141 
   Size:       7837
 
DraIII CAC_nnn'GTG

Cuts at:   3451    3666    5116    3451 
   Size:        215    1450    6172

  Fragments arranged by size: 

                6172    1450     215
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6844     809 
   Size:        507    5528    1802

  Fragments arranged by size: 

                5528    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2454    3716    3918 
   Size:         54    1425      50     275      18     624    1262     202

Cuts at:   3918    3934    4656    4741    5284    6173    7802       8 
   Size:         16     722      85     543     889    1629      43

  Fragments arranged by size: 

                1629    1425    1262     889     722     624     543     275
                 202      85      54      50      43      18      16
 
EagI C'GGCC_G

Cuts at:   1537    1812    2454    5284    1537 
   Size:        275     642    2830    4090

  Fragments arranged by size: 

                4090    2830     642     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2183    4928    5767    1353 
   Size:        830    2745     839    3423

  Fragments arranged by size: 

                3423    2745     839     830
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3260    6424    6798    6944     387 
   Size:       1064     138    1671    3164     374     146    1280

  Fragments arranged by size: 

                3164    1671    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2983    4110    4969    2983 
   Size:       1127     859    5851

  Fragments arranged by size: 

                5851    1127     859
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2270    2537    2981    3149    3488    3569    3742 
   Size:        393     638     267     444     168     339      81     173

Cuts at:   3742    3764    4028    4100    4251    4601    5060    5900    6497 
   Size:         22     264      72     151     350     459     840     597

Cuts at:   6497    7284    7552    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     840     787     638     597     459     444     393
                 350     339     268     267     264     173     168     151
                  81      72      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3431    4018    4358    4478    3431 
   Size:        587     340     120    6790

  Fragments arranged by size: 

                6790     587     340     120
 
EcoO109I rG'GnC_Cy

Cuts at:   2540    2569    3589    3913    4084    4183    4603    4890    2540 
   Size:         29    1020     324     171      99     420     287    5487

  Fragments arranged by size: 

                5487    1020     420     324     287     171      99      29
            
 
EcoRI G'AATT_C

Cuts at:   2033    5146    5524    2033 
   Size:       3113     378    4346

  Fragments arranged by size: 

                4346    3113     378
 
FspI TGC'GCA

Cuts at:   3383    6343    3383 
   Size:       2960    4877
 
HaeII r_GCGC'y

Cuts at:   1678    2651    3408    3641    3741    4912    6984    1678 
   Size:        973     757     233     100    1171    2072    2531

  Fragments arranged by size: 

                2531    2072    1171     973     757     233     100
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    6478    6510    6552    6584    1373 
   Size:         32     135      32    4906      32      42      32    2626

  Fragments arranged by size: 

                4906    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2071    2581    2814    5181    5427     669 
   Size:        988     281     133     510     233    2367     246    3079

  Fragments arranged by size: 

                3079    2367     988     510     281     246     233     133
            
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       7837
 
HpaI GTT'AAC

Cuts at:   5427    5427 
   Size:       7837
 
KasI G'GCGC_C

Cuts at:   4908    4908 
   Size:       7837
 
KpnI G_GTAC'C

Cuts at:   2550    5162    2550 
   Size:       2612    5225
 
MfeI C'AATT_G

Cuts at:   5436    5436 
   Size:       7837
 
MluI A'CGCG_T

Cuts at:   7713    7713 
   Size:       7837
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4540    6951    7135    1305 
   Size:       3235    2411     184    2007

  Fragments arranged by size: 

                3235    2411    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3920    4658    4743      10 
   Size:         54    1425     343    2088     738      85    3104

  Fragments arranged by size: 

                3104    2088    1425     738     343      85      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3681    4043    4256    4481    4664    5795    6154 
   Size:        648    2515     362     213     225     183    1131     359

Cuts at:   6154    6313     518 
   Size:        159    2042

  Fragments arranged by size: 

                2515    2042    1131     648     362     359     225     213
                 183     159
 
MspA1I CmG'CkG

Cuts at:   1606    2146    2234    2473    2515    2644    2698    2796    3124 
   Size:        540      88     239      42     129      54      98     328

Cuts at:   3124    3410    3529    3535    3847    4314    4723    4974    5253 
   Size:        286     119       6     312     467     409     251     279

Cuts at:   5253    5930    7078    7323    1606 
   Size:        677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     540     467     409     328     312
                 286     279     251     245     239     129     119      98
                  88      54      42       6
 
NaeI GCC'GGC

Cuts at:   2465    2465 
   Size:       7837
 
NarI GG'CG_CC

Cuts at:   4909    4909 
   Size:       7837
 
NcoI C'CATG_G

Cuts at:    513    1065    2028    2829     513 
   Size:        552     963     801    5521

  Fragments arranged by size: 

                5521     963     801     552
 
NdeI CA'TA_TG

Cuts at:    387    4663     387 
   Size:       4276    3561
 
NgoMIV G'CCGG_C

Cuts at:   2463    2463 
   Size:       7837
 
NheI G'CTAG_C

Cuts at:   1052    3640    1052 
   Size:       2588    5249
 
NotI GC'GGCC_GC

Cuts at:   2454    5284    2454 
   Size:       2830    5007
 
NspI r_CATG'y

Cuts at:   1132    1229    2188    3328    3512    4162    4369    5195    6740 
   Size:         97     959    1140     184     650     207     826    1545

Cuts at:   6740    1132 
   Size:       2229

  Fragments arranged by size: 

                2229    1545    1140     959     826     650     207     184
                  97
 
PasI CC'CwG_GG

Cuts at:   1348    2012    1348 
   Size:        664    7173
 
PciI A'CATG_T

Cuts at:   3324    6736    3324 
   Size:       3412    4425
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3261    4393    4750    1108 
   Size:       2153    1132     357    4195

  Fragments arranged by size: 

                4195    2153    1132     357
 
PfoI T'CCnGG_A

Cuts at:   3546    3618    3630    4005    3546 
   Size:         72      12     375    7378

  Fragments arranged by size: 

                7378     375      72      12
 
PmeI GTTT'AAAC

Cuts at:   5141    5141 
   Size:       7837
 
PmlI CAC'GTG

Cuts at:   3973    3973 
   Size:       7837
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2954    2986    3303    3335    5906    5938    7419    7451    2954 
   Size:         32     317      32    2571      32    1481      32    3340

  Fragments arranged by size: 

                3340    2571    1481     317      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:   3589    3589 
   Size:       7837
 
PshAI GACnn'nnGTC

Cuts at:   1613    2831    1613 
   Size:       1218    6619
 
PsiI TTA'TAA

Cuts at:   5407    5407 
   Size:       7837
 
PspOMI G'GGCC_C

Cuts at:   2568    3912    2568 
   Size:       1344    6493
 
PstI C_TGCA'G

Cuts at:    830    2496    2514    2730    2795    3534    3846    4022    4200 
   Size:       1666      18     216      65     739     312     176     178

Cuts at:   4200    4380    5189     830 
   Size:        180     809    3478

  Fragments arranged by size: 

                3478    1666     809     739     312     216     180     178
                 176      65      18
 
PvuI CG_AT'CG

Cuts at:   2001    4684    2001 
   Size:       2683    5154
 
PvuII CAG'CTG

Cuts at:   2146    2473    2515    2796    3529    3535    3847    4723    4974 
   Size:        327      42     281     733       6     312     876     251

Cuts at:   4974    2146 
   Size:       5009

  Fragments arranged by size: 

                5009     876     733     327     312     281     251      42
                   6
 
SacI G_AGCT'C

Cuts at:   2985    4112    4971    2985 
   Size:       1127     859    5851

  Fragments arranged by size: 

                5851    1127     859
 
SalI G'TCGA_C

Cuts at:   1936    5179    1936 
   Size:       3243    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2183    2183 
   Size:       7837
 
SbfI CC_TGCA'GG

Cuts at:   5189    5189 
   Size:       7837
 
ScaI AGT'ACT

Cuts at:   6085    7797    6085 
   Size:       1712    6125
 
SexAI A'CCwGG_T

Cuts at:   3591    3591 
   Size:       7837
 
SfcI C'TryA_G

Cuts at:    826    1045    2492    2510    2726    2791    3530    3842    4018 
   Size:        219    1447      18     216      65     739     312     176

Cuts at:   4018    4196    4376    5185    6320    7001    7192     826 
   Size:        178     180     809    1135     681     191    1471

  Fragments arranged by size: 

                1471    1447    1135     809     739     681     312     219
                 216     191     180     178     176      65      18
 
SfoI GGC'GCC

Cuts at:   4910    4910 
   Size:       7837
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7837
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7837
 
SmaI CCC'GGG

Cuts at:   1872    4596    5164    1872 
   Size:       2724     568    4545

  Fragments arranged by size: 

                4545    2724     568
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3299    3860    4997    5941    6842    7104 
   Size:        197     926    1356     561    1137     944     901     262

Cuts at:   7104     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1356    1137     944     926     901     561     262
                 197
 
SnaBI TAC'GTA

Cuts at:    493    5628     493 
   Size:       5135    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7837
 
SphI G_CATG'C

Cuts at:   1132    4162    5195    1132 
   Size:       3030    1033    3774

  Fragments arranged by size: 

                3774    3030    1033
 
SspI AAT'ATT

Cuts at:      5      52    5761       5 
   Size:         47    5709    2081

  Fragments arranged by size: 

                5709    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2204    2376    2486    4013    4172    4473    4959    1517 
   Size:        687     172     110    1527     159     301     486    4395

  Fragments arranged by size: 

                4395    1527     687     486     301     172     159     110
            
 
StyI C'CwwG_G

Cuts at:    513    1065    2028    2043    2631    2829    4137    5276     513 
   Size:        552     963      15     588     198    1308    1139    3074

  Fragments arranged by size: 

                3074    1308    1139     963     588     552     198      15
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4046    5864    5881    6034    6219    6664     325 
   Size:       1255    2466    1818      17     153     185     445    1498

  Fragments arranged by size: 

                2466    1818    1498    1255     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2496    2784    3453 
   Size:        274      80      33      51    1443     519     288     669

Cuts at:   3453    4290    6083    7795      96 
   Size:        837    1793    1712     138

  Fragments arranged by size: 

                1793    1712    1443     837     669     519     288     274
                 138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3098    3130    3581    3613    3767    3799    3944    3976    4184 
   Size:         32     451      32     154      32     145      32     208

Cuts at:   4184    4216    4388    4420    5102    5134    7797    7829    3098 
   Size:         32     172      32     682      32    2663      32    3106

  Fragments arranged by size: 

                3106    2663     682     451     208     172     154     145
                  32      32      32      32      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    3076    4097    4817    1316 
   Size:       1760    1021     720    4336

  Fragments arranged by size: 

                4336    1760    1021     720
 
XbaI T'CTAG_A

Cuts at:   5173    5173 
   Size:       7837
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2189    3901    4222    4666    4736    2189 
   Size:       1712     321     444      70    5290

  Fragments arranged by size: 

                5290    1712     444     321      70
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7837
 
XmaI C'CCGG_G

Cuts at:   1870    4594    5162    1870 
   Size:       2724     568    4545

  Fragments arranged by size: 

                4545    2724     568
 
XmnI GAAnn'nnTTC

Cuts at:   4094    5045    5966    4094 
   Size:        951     921    5965

  Fragments arranged by size: 

                5965     951     921
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4399    5645     276 
   Size:         53      83     186     936    2865    1246    2468

  Fragments arranged by size: 

                2865    2468    1246     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI
    PvuII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AscI    AvrII     BaeI    BfrBI    BsiWI    BstXI  BstZ17I
    EcoRV     FalI     FseI    FspAI     NruI     NsiI     PacI    PspXI
     PsrI    RsrII    SacII    SanDI     SfiI     SrfI     SwaI
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