(Linear) (Six-base) MAPSORT of: KIEE3353.seq Check: 8875 from: 1 to: 3132 KIEE3353.orf With 182 enzymes: SgfI * July 3, 2012 17:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 220 714 1839 2198 3132 Size: 220 494 1125 359 934 Fragments arranged by size: 1125 934 494 359 220 AatII G_ACGT'C Cuts at: 0 2396 3132 Size: 2396 736 AccI GT'mk_AC Cuts at: 0 89 3132 Size: 89 3043 Acc65I G'GTAC_C Cuts at: 0 541 3132 Size: 541 2591 AclI AA'CG_TT Cuts at: 0 578 3132 Size: 578 2554 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1737 2095 2246 2596 3055 3132 Size: 1737 358 151 350 459 77 Fragments arranged by size: 1737 459 358 350 151 77 AflII C'TTAA_G Cuts at: 0 1294 3132 Size: 1294 1838 AflIII A'CryG_T Cuts at: 0 1319 3110 3132 Size: 1319 1791 22 Fragments arranged by size: 1791 1319 22 AleI CACnn'nnGTG Cuts at: 0 2659 3132 Size: 2659 473 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 811 1072 3132 Size: 811 261 2060 Fragments arranged by size: 2060 811 261 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 949 981 3132 Size: 949 32 2151 Fragments arranged by size: 2151 949 32 AlwNI CAG_nnn'CTG Cuts at: 0 144 468 693 1122 1833 3132 Size: 144 324 225 429 711 1299 Fragments arranged by size: 1299 711 429 324 225 144 ApaI G_GGCC'C Cuts at: 0 567 1911 3132 Size: 567 1344 1221 Fragments arranged by size: 1344 1221 567 ApaLI G'TGCA_C Cuts at: 0 2244 3132 Size: 2244 888 ApoI r'AATT_y Cuts at: 0 28 1891 2751 2854 3132 Size: 28 1863 860 103 278 Fragments arranged by size: 1863 860 278 103 28 AvaI C'yCGr_G Cuts at: 0 193 1426 2589 3132 Size: 193 1233 1163 543 Fragments arranged by size: 1233 1163 543 193 BanI G'GyrC_C Cuts at: 0 149 541 1946 2903 3132 Size: 149 392 1405 957 229 Fragments arranged by size: 1405 957 392 229 149 BanII G_rGCy'C Cuts at: 0 518 567 980 1106 1911 2107 2966 3132 Size: 518 49 413 126 805 196 859 166 Fragments arranged by size: 859 805 518 413 196 166 126 49 BbeI G_GCGC'C Cuts at: 0 2907 3132 Size: 2907 225 BbsI GAAGACnn'nnnn_ Cuts at: 0 716 2119 2842 3132 Size: 716 1403 723 290 Fragments arranged by size: 1403 723 716 290 BbvCI CC'TCA_GC Cuts at: 0 124 3132 Size: 124 3008 BglI GCCn_nnn'nGGC Cuts at: 0 1207 1775 3132 Size: 1207 568 1357 Fragments arranged by size: 1357 1207 568 BglII A'GATC_T Cuts at: 0 1469 3132 Size: 1469 1663 BlpI GC'TnA_GC Cuts at: 0 2101 2965 3132 Size: 2101 864 167 Fragments arranged by size: 2101 864 167 Bme1580I G_kGCm'C Cuts at: 0 567 660 1372 1911 2248 3132 Size: 567 93 712 539 337 884 Fragments arranged by size: 884 712 567 539 337 93 BmgBI CAC'GTC Cuts at: 0 273 633 3132 Size: 273 360 2499 Fragments arranged by size: 2499 360 273 BmrI ACTGGGnnnn_n' Cuts at: 0 153 3019 3132 Size: 153 2866 113 Fragments arranged by size: 2866 153 113 BmtI G_CTAG'C Cuts at: 0 1639 3132 Size: 1639 1493 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 402 2199 3132 Size: 402 1797 933 Fragments arranged by size: 1797 933 402 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 532 976 1144 1483 1564 1759 2023 Size: 265 267 444 168 339 81 195 264 Cuts at: 2023 3132 Size: 1109 Fragments arranged by size: 1109 444 339 267 265 264 195 168 81 Bpu10I CC'TnA_GC Cuts at: 0 124 1729 2960 3132 Size: 124 1605 1231 172 Fragments arranged by size: 1605 1231 172 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1840 2977 3132 Size: 1840 1137 155 Fragments arranged by size: 1840 1137 155 BsaAI yAC'GTr Cuts at: 0 1773 1968 3132 Size: 1773 195 1164 Fragments arranged by size: 1773 1164 195 BsaHI Gr'CG_yC Cuts at: 0 261 2393 2904 3132 Size: 261 2132 511 228 Fragments arranged by size: 2132 511 261 228 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 949 979 1093 1123 1762 1792 1939 1969 Size: 949 30 114 30 639 30 147 30 Cuts at: 1969 3132 Size: 1163 Fragments arranged by size: 1163 949 639 147 114 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 58 346 457 613 828 1528 1531 2311 Size: 58 288 111 156 215 700 3 780 Cuts at: 2311 2632 3037 3040 3132 Size: 321 405 3 92 Fragments arranged by size: 780 700 405 321 288 215 156 111 92 58 3 3 BseYI C'CCAG_C Cuts at: 0 468 473 1479 2209 2664 3132 Size: 468 5 1006 730 455 468 Fragments arranged by size: 1006 730 468 468 455 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 781 1048 1867 3132 Size: 295 486 267 819 1265 Fragments arranged by size: 1265 819 486 295 267 BsiEI CG_ry'CG Cuts at: 0 321 452 2679 3132 Size: 321 131 2227 453 Fragments arranged by size: 2227 453 321 131 BsiHKAI G_wGCw'C Cuts at: 0 980 1099 1577 1622 1708 1856 2107 2248 Size: 980 119 478 45 86 148 251 141 Cuts at: 2248 2966 3132 Size: 718 166 Fragments arranged by size: 980 718 478 251 166 148 141 119 86 45 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1171 3132 Size: 1171 1961 Bsp1286I G_dGCh'C Cuts at: 0 518 567 660 980 1099 1106 1372 1577 Size: 518 49 93 320 119 7 266 205 Cuts at: 1577 1622 1708 1856 1911 2107 2248 2966 3132 Size: 45 86 148 55 196 141 718 166 Fragments arranged by size: 718 518 320 266 205 196 166 148 141 119 93 86 55 49 45 7 BspHI T'CATG_A Cuts at: 0 2696 3132 Size: 2696 436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 220 714 1157 1706 1839 1841 2198 3132 Size: 220 494 443 549 133 2 357 934 Fragments arranged by size: 934 549 494 443 357 220 133 2 BsrBI CCG'CTC Cuts at: 0 171 765 1011 1548 1955 3132 Size: 171 594 246 537 407 1177 Fragments arranged by size: 1177 594 537 407 246 171 BsrDI GCAATG_nn' Cuts at: 0 120 1250 3132 Size: 120 1130 1882 Fragments arranged by size: 1882 1130 120 BsrFI r'CCGG_y Cuts at: 0 458 3132 Size: 458 2674 BsrGI T'GTAC_A Cuts at: 0 2285 3132 Size: 2285 847 BssSI C'ACGA_G Cuts at: 0 376 1576 3132 Size: 376 1200 1556 Fragments arranged by size: 1556 1200 376 BstAPI GCAn_nnn'nTGC Cuts at: 0 1845 2361 3132 Size: 1845 516 771 Fragments arranged by size: 1845 771 516 BstEII G'GTnAC_C Cuts at: 0 1653 3132 Size: 1653 1479 BstYI r'GATC_y Cuts at: 0 1469 3132 Size: 1469 1663 Bsu36I CC'TnA_GG Cuts at: 0 2422 3132 Size: 2422 710 BtgI C'CryG_G Cuts at: 0 23 824 1044 1364 2907 3132 Size: 23 801 220 320 1543 225 Fragments arranged by size: 1543 801 320 225 220 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2484 3132 Size: 2484 648 BtsI GCAGTG_nn' Cuts at: 0 2248 3132 Size: 2248 884 DraIII CAC_nnn'GTG Cuts at: 0 1446 1661 3111 3132 Size: 1446 215 1450 21 Fragments arranged by size: 1450 1446 215 21 EaeI y'GGCC_r Cuts at: 0 449 1711 1913 1929 2651 2736 3132 Size: 449 1262 202 16 722 85 396 Fragments arranged by size: 1262 722 449 396 202 85 16 EagI C'GGCC_G Cuts at: 0 449 3132 Size: 449 2683 EarI CTCTTCn'nnn_ Cuts at: 0 178 2923 3132 Size: 178 2745 209 Fragments arranged by size: 2745 209 178 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1255 3132 Size: 1255 1877 EcoICRI GAG'CTC Cuts at: 0 978 2105 2964 3132 Size: 978 1127 859 168 Fragments arranged by size: 1127 978 859 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 532 976 1144 1483 1564 1737 1759 Size: 265 267 444 168 339 81 173 22 Cuts at: 1759 2023 2095 2246 2596 3055 3132 Size: 264 72 151 350 459 77 Fragments arranged by size: 459 444 350 339 267 265 264 173 168 151 81 77 72 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 1426 2013 2353 2473 3132 Size: 1426 587 340 120 659 Fragments arranged by size: 1426 659 587 340 120 EcoO109I rG'GnC_Cy Cuts at: 0 535 564 1584 1908 2079 2178 2598 2885 Size: 535 29 1020 324 171 99 420 287 Cuts at: 2885 3132 Size: 247 Fragments arranged by size: 1020 535 420 324 287 247 171 99 29 EcoRI G'AATT_C Cuts at: 0 28 3132 Size: 28 3104 FspI TGC'GCA Cuts at: 0 1378 3132 Size: 1378 1754 HaeII r_GCGC'y Cuts at: 0 646 1403 1636 1736 2907 3132 Size: 646 757 233 100 1171 225 Fragments arranged by size: 1171 757 646 233 225 100 HincII GTy'rAC Cuts at: 0 66 576 809 3132 Size: 66 510 233 2323 Fragments arranged by size: 2323 510 233 66 KasI G'GCGC_C Cuts at: 0 2903 3132 Size: 2903 229 KpnI G_GTAC'C Cuts at: 0 545 3132 Size: 545 2587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2535 3132 Size: 2535 597 MscI TGG'CCA Cuts at: 0 1915 2653 2738 3132 Size: 1915 738 85 394 Fragments arranged by size: 1915 738 394 85 MslI CAynn'nnrTG Cuts at: 0 1676 2038 2251 2476 2659 3132 Size: 1676 362 213 225 183 473 Fragments arranged by size: 1676 473 362 225 213 183 MspA1I CmG'CkG Cuts at: 0 141 229 468 510 639 693 791 1119 Size: 141 88 239 42 129 54 98 328 Cuts at: 1119 1405 1524 1530 1842 2309 2718 2969 3132 Size: 286 119 6 312 467 409 251 163 Fragments arranged by size: 467 409 328 312 286 251 239 163 141 129 119 98 88 54 42 6 NaeI GCC'GGC Cuts at: 0 460 3132 Size: 460 2672 NarI GG'CG_CC Cuts at: 0 2904 3132 Size: 2904 228 NcoI C'CATG_G Cuts at: 0 23 824 3132 Size: 23 801 2308 Fragments arranged by size: 2308 801 23 NdeI CA'TA_TG Cuts at: 0 2658 3132 Size: 2658 474 NgoMIV G'CCGG_C Cuts at: 0 458 3132 Size: 458 2674 NheI G'CTAG_C Cuts at: 0 1635 3132 Size: 1635 1497 NotI GC'GGCC_GC Cuts at: 0 449 3132 Size: 449 2683 NspI r_CATG'y Cuts at: 0 183 1323 1507 2157 2364 3132 Size: 183 1140 184 650 207 768 Fragments arranged by size: 1140 768 650 207 184 183 PasI CC'CwG_GG Cuts at: 0 7 3132 Size: 7 3125 PciI A'CATG_T Cuts at: 0 1319 3132 Size: 1319 1813 PflMI CCAn_nnn'nTGG Cuts at: 0 1256 2388 2745 3132 Size: 1256 1132 357 387 Fragments arranged by size: 1256 1132 387 357 PfoI T'CCnGG_A Cuts at: 0 1541 1613 1625 2000 3132 Size: 1541 72 12 375 1132 Fragments arranged by size: 1541 1132 375 72 12 PmlI CAC'GTG Cuts at: 0 1968 3132 Size: 1968 1164 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 949 981 1298 1330 3132 Size: 949 32 317 32 1802 Fragments arranged by size: 1802 949 317 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1584 3132 Size: 1584 1548 PshAI GACnn'nnGTC Cuts at: 0 826 3132 Size: 826 2306 PspOMI G'GGCC_C Cuts at: 0 563 1907 3132 Size: 563 1344 1225 Fragments arranged by size: 1344 1225 563 PstI C_TGCA'G Cuts at: 0 491 509 725 790 1529 1841 2017 2195 Size: 491 18 216 65 739 312 176 178 Cuts at: 2195 2375 3132 Size: 180 757 Fragments arranged by size: 757 739 491 312 216 180 178 176 65 18 PvuI CG_AT'CG Cuts at: 0 2679 3132 Size: 2679 453 PvuII CAG'CTG Cuts at: 0 141 468 510 791 1524 1530 1842 2718 Size: 141 327 42 281 733 6 312 876 Cuts at: 2718 2969 3132 Size: 251 163 Fragments arranged by size: 876 733 327 312 281 251 163 141 42 6 SacI G_AGCT'C Cuts at: 0 980 2107 2966 3132 Size: 980 1127 859 166 Fragments arranged by size: 1127 980 859 166 SapI GCTCTTCn'nnn_ Cuts at: 0 178 3132 Size: 178 2954 SexAI A'CCwGG_T Cuts at: 0 1586 3132 Size: 1586 1546 SfcI C'TryA_G Cuts at: 0 487 505 721 786 1525 1837 2013 2191 Size: 487 18 216 65 739 312 176 178 Cuts at: 2191 2371 3132 Size: 180 761 Fragments arranged by size: 761 739 487 312 216 180 178 176 65 18 SfoI GGC'GCC Cuts at: 0 2905 3132 Size: 2905 227 SmaI CCC'GGG Cuts at: 0 2591 3132 Size: 2591 541 SmlI C'TyrA_G Cuts at: 0 1294 1855 2992 3132 Size: 1294 561 1137 140 Fragments arranged by size: 1294 1137 561 140 SphI G_CATG'C Cuts at: 0 2157 3132 Size: 2157 975 StuI AGG'CCT Cuts at: 0 199 371 481 2008 2167 2468 2954 3132 Size: 199 172 110 1527 159 301 486 178 Fragments arranged by size: 1527 486 301 199 178 172 159 110 StyI C'CwwG_G Cuts at: 0 23 38 626 824 2132 3132 Size: 23 15 588 198 1308 1000 Fragments arranged by size: 1308 1000 588 198 23 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2041 3132 Size: 2041 1091 TatI w'GTAC_w Cuts at: 0 491 779 1448 2285 3132 Size: 491 288 669 837 847 Fragments arranged by size: 847 837 669 491 288 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1093 1125 1576 1608 1762 1794 1939 1971 Size: 1093 32 451 32 154 32 145 32 Cuts at: 1971 2179 2211 2383 2415 3097 3129 3132 Size: 208 32 172 32 682 32 3 Fragments arranged by size: 1093 682 451 208 172 154 145 32 32 32 32 32 32 32 3 Tth111I GACn'n_nGTC Cuts at: 0 1071 2092 2812 3132 Size: 1071 1021 720 320 Fragments arranged by size: 1071 1021 720 320 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 184 1896 2217 2661 2731 3132 Size: 184 1712 321 444 70 401 Fragments arranged by size: 1712 444 401 321 184 70 XmaI C'CCGG_G Cuts at: 0 2589 3132 Size: 2589 543 XmnI GAAnn'nnTTC Cuts at: 0 2089 3040 3132 Size: 2089 951 92 Fragments arranged by size: 2089 951 92 ZraI GAC'GTC Cuts at: 0 2394 3132 Size: 2394 738 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII SacI SapI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AfeI AgeI AhdI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BsaI BsaBI BsaWI BsiWI BsmI BspEI BssHII BstBI BstXI BstZ17I ClaI CspCI DraI DrdI EcoRV FalI FseI FspAI Hin4I HindIII HpaI MfeI MluI NruI NsiI PacI PmeI PsiI PspXI PsrI RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI