Restriction map for
pFN21AE3552
(Circular) (Six-base) MAPSORT of: pFN21AE3552.seq Check: 6381 from: 1 to: 10579
pFN21AE3552 10579 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 3452 8389 278
Size: 53 83 186 936 1786 130 4937 2468
Fragments arranged by size:
4937 2468 1786 936 186 130 83 53
AccI GT'mk_AC
Cuts at: 1937 6075 6811 7922 1937
Size: 4138 736 1111 4594
Fragments arranged by size:
4594 4138 1111 736
Acc65I G'GTAC_C
Cuts at: 7900 7900
Size: 10579
AclI AA'CG_TT
Cuts at: 1563 8706 9079 1563
Size: 7143 373 3063
Fragments arranged by size:
7143 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2139 2919 4095 4530 4733 4774 5091 5668
Size: 507 780 1176 435 203 41 317 577
Cuts at: 5668 5867 6057 6525 6936 7032 7063 8642 10026
Size: 199 190 468 411 96 31 1579 1384
Cuts at: 10026 10294 1632
Size: 268 1917
Fragments arranged by size:
1917 1579 1384 1176 780 577 507 468
435 411 317 268 203 199 190 96
41 31
AflII C'TTAA_G
Cuts at: 820 1017 3041 5825 820
Size: 197 2024 2784 5574
Fragments arranged by size:
5574 2784 2024 197
AflIII A'CryG_T
Cuts at: 1203 2015 7341 9478 10455 1203
Size: 812 5326 2137 977 1327
Fragments arranged by size:
5326 2137 1327 977 812
AgeI A'CCGG_T
Cuts at: 7512 9410 7512
Size: 1898 8681
AhdI GACnn_n'nnGTC
Cuts at: 4759 4832 9308 4759
Size: 73 4476 6030
Fragments arranged by size:
6030 4476 73
AleI CACnn'nnGTG
Cuts at: 1166 2792 1166
Size: 1626 8953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3323 6242 7446 1184
Size: 2139 2919 1204 4317
Fragments arranged by size:
4317 2919 2139 1204
AlwNI CAG_nnn'CTG
Cuts at: 2225 6635 8322 9894 2225
Size: 4410 1687 1572 2910
Fragments arranged by size:
4410 2910 1687 1572
ApaLI G'TGCA_C
Cuts at: 8636 9792 8636
Size: 1156 9423
ApoI r'AATT_y
Cuts at: 1493 3033 3139 3648 3759 3796 5863 7006 7453
Size: 1540 106 509 111 37 2067 1143 447
Cuts at: 7453 7888 8114 8266 10375 1493
Size: 435 226 152 2109 1697
Fragments arranged by size:
2109 2067 1697 1540 1143 509 447 435
226 152 111 106 37
AseI AT'TA_AT
Cuts at: 160 1032 4556 5492 9133 160
Size: 872 3524 936 3641 1606
Fragments arranged by size:
3641 3524 1606 936 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10579
AvaI C'yCGr_G
Cuts at: 1870 1943 3828 4378 7904 1870
Size: 73 1885 550 3526 4545
Fragments arranged by size:
4545 3526 1885 550 73
AvrII C'CTAG_G
Cuts at: 3635 3635
Size: 10579
BamHI G'GATC_C
Cuts at: 7909 8276 7909
Size: 367 10212
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7900 9355 618
Size: 325 217 627 6113 1455 1842
Fragments arranged by size:
6113 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 6587 6821 7330 7898 1391
Size: 5196 234 509 568 4072
Fragments arranged by size:
5196 4072 568 509 234
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2490 4758 4780 5079 5855 5943 6654
Size: 971 591 2268 22 299 776 88 711
Cuts at: 6654 928
Size: 4853
Fragments arranged by size:
4853 2268 971 776 711 591 299 88
22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8770 8804 1903
Size: 34 6833 34 3678
Fragments arranged by size:
6833 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2296 8471 9681 2296
Size: 6175 1210 3194
Fragments arranged by size:
6175 3194 1210
BclI T'GATC_A
Cuts at: 1548 4450 1548
Size: 2902 7677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4258 6677
Size: 107 122 71 631 605 337 2249 2419
Cuts at: 6677 9190 136
Size: 2513 1525
Fragments arranged by size:
2513 2419 2249 1525 631 605 337 122
107 71
BglII A'GATC_T
Cuts at: 3089 3769 10574 3089
Size: 680 6805 3094
Fragments arranged by size:
6805 3094 680
BlpI GC'TnA_GC
Cuts at: 4614 6653 7531 7996 4614
Size: 2039 878 465 7197
Fragments arranged by size:
7197 2039 878 465
Bme1580I G_kGCm'C
Cuts at: 1790 3348 4021 7425 8640 9796 1790
Size: 1558 673 3404 1215 1156 2573
Fragments arranged by size:
3404 2573 1558 1215 1156 673
BmgBI CAC'GTC
Cuts at: 1318 4337 5043 6660 1318
Size: 3019 706 1617 5237
Fragments arranged by size:
5237 3019 1617 706
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3343 4050 6407
Size: 416 53 83 399 24 1926 707 2357
Cuts at: 6407 7242 8310 9268 442
Size: 835 1068 958 1753
Fragments arranged by size:
2357 1926 1753 1068 958 835 707 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5093 1056
Size: 4037 6542
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 3127 6649 2947
Size: 180 3522 6877
Fragments arranged by size:
6877 3522 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2995 4083 5163 6790 9239 1239
Size: 1756 1088 1080 1627 2449 2579
Fragments arranged by size:
2579 2449 1756 1627 1088 1080
Bpu10I CC'TnA_GC
Cuts at: 5697 7598 5697
Size: 1901 8678
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 2160 2523 3064 3078 3420 5566 8704 9569
Size: 121 363 541 14 342 2146 3138 865
Cuts at: 9569 9867 2039
Size: 298 2751
Fragments arranged by size:
3138 2751 2146 865 541 363 342 298
121 14
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 4289 5003 7312 9242 882
Size: 617 575 2215 714 2309 1930 2219
Fragments arranged by size:
2309 2219 2215 1930 714 617 575
BsaAI yAC'GTr
Cuts at: 493 1204 4702 8370 493
Size: 711 3498 3668 2702
Fragments arranged by size:
3668 3498 2702 711
BsaBI GATnn'nnATC
Cuts at: 4642 4994 10573 4642
Size: 352 5579 4648
Fragments arranged by size:
5579 4648 352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3319 3449 4237 5442
Size: 53 83 186 936 1786 130 788 1205
Cuts at: 5442 8386 8768 9403 275
Size: 2944 382 635 1451
Fragments arranged by size:
2944 1786 1451 1205 936 788 635 382
186 130 83 53
BsaWI w'CCGG_w
Cuts at: 7512 9012 9410 9684 9831 10242 7512
Size: 1500 398 274 147 411 7849
Fragments arranged by size:
7849 1500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2382 2412 3670 3700 4286
Size: 30 165 30 979 30 1258 30 586
Cuts at: 4286 4316 4513 4534 4543 4564 1178
Size: 30 197 21 9 21 7193
Fragments arranged by size:
7193 1258 979 586 197 165 30 30
30 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2283 2658 3744 6386 7620 1186
Size: 1097 375 1086 2642 1234 4145
Fragments arranged by size:
4145 2642 1234 1097 1086 375
BseYI C'CCAG_C
Cuts at: 6081 6452 9782 10278 6081
Size: 371 3330 496 6382
Fragments arranged by size:
6382 3330 496 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6077 1163
Size: 573 4341 5665
Fragments arranged by size:
5665 4341 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7662 8029 8790 9818 1540
Size: 275 186 5661 367 761 1028 2301
Fragments arranged by size:
5661 2301 1028 761 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 3445 6497 8640 8725 9796 3445
Size: 3052 2143 85 1071 4228
Fragments arranged by size:
4228 3052 2143 1071 85
BsmI GAATG_Cn'
Cuts at: 5060 5382 7375 7822 8090 8183 5060
Size: 322 1993 447 268 93 7456
Fragments arranged by size:
7456 1993 447 322 268 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 10579
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3348 3445 4021 6497 6587 6821 7330
Size: 399 1558 97 576 2476 90 234 509
Cuts at: 7330 7425 7898 8640 8725 9796 1391
Size: 95 473 742 85 1071 2174
Fragments arranged by size:
2476 2174 1558 1071 742 576 509 473
399 234 97 95 90 85
BspEI T'CCGG_A
Cuts at: 10242 10242
Size: 10579
BspHI T'CATG_A
Cuts at: 3334 4453 5394 8468 10198 3334
Size: 1119 941 3074 1730 3715
Fragments arranged by size:
3715 3074 1730 1119 941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2347 4322 7934 844
Size: 1503 1975 3612 3489
Fragments arranged by size:
3612 3489 1975 1503
BsrBI CCG'CTC
Cuts at: 1394 1823 7479 8466 1394
Size: 429 5656 987 3507
Fragments arranged by size:
5656 3507 987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2584 2812 3779 6420 7681 9074 9248
Size: 1183 1336 228 967 2641 1261 1393 174
Cuts at: 9248 65
Size: 1396
Fragments arranged by size:
2641 1396 1393 1336 1261 1183 967 228
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7512 9223 9410 1700
Size: 110 5702 1711 187 2869
Fragments arranged by size:
5702 2869 1711 187 110
BsrGI T'GTAC_A
Cuts at: 96 2360 4920 96
Size: 2264 2560 5755
Fragments arranged by size:
5755 2560 2264
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10579
BssSI C'ACGA_G
Cuts at: 7644 8639 9651 7644
Size: 995 1012 8572
Fragments arranged by size:
8572 1012 995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5257 7817 794
Size: 4463 2560 3556
Fragments arranged by size:
4463 3556 2560
BstBI TT'CG_AA
Cuts at: 5926 9383 5926
Size: 3457 7122
BstEII G'GTnAC_C
Cuts at: 1184 2366 3849 5956 1184
Size: 1182 1483 2107 5807
Fragments arranged by size:
5807 2107 1483 1182
BstXI CCAn_nnnn'nTGG
Cuts at: 3019 7711 3019
Size: 4692 5887
BstYI r'GATC_y
Cuts at: 1972 3089 3737 3769 5158 5755 6197 7618 7909
Size: 1117 648 32 1389 597 442 1421 291
Cuts at: 7909 8276 8661 8678 10130 10216 10228 10574 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1421 1389 1117 648 597 442
385 367 346 291 86 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 4214 6822 4214
Size: 2608 7971
BtgI C'CryG_G
Cuts at: 513 1065 2643 4874 6834 513
Size: 552 1578 2231 1960 4258
Fragments arranged by size:
4258 2231 1960 1578 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9840
Fragments arranged by size:
9840 683 56
BtsI GCAGTG_nn'
Cuts at: 2799 3379 3821 8095 8889 8909 2799
Size: 580 442 4274 794 20 4469
Fragments arranged by size:
4469 4274 794 580 442 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10579
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3571 3606 529
Size: 35 3007 35 7502
Fragments arranged by size:
7502 3007 35 35
DraI TTT'AAA
Cuts at: 2937 3754 7883 2937
Size: 817 4129 5633
Fragments arranged by size:
5633 4129 817
DraIII CAC_nnn'GTG
Cuts at: 7366 7366
Size: 10579
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2029 4948 5144 9586 809
Size: 507 713 2919 196 4442 1802
Fragments arranged by size:
4442 2919 1802 713 507 196
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2138 4871
Size: 54 1425 50 275 18 180 128 2733
Cuts at: 4871 6735 7563 8026 8915 10544 8
Size: 1864 828 463 889 1629 43
Fragments arranged by size:
2733 1864 1629 1425 889 828 463 275
180 128 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8026 1537
Size: 275 6214 4090
Fragments arranged by size:
6214 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4953 5127 5304 5711 6033 6618 7082 7683
Size: 3600 174 177 407 322 585 464 601
Cuts at: 7683 8509 1353
Size: 826 3423
Fragments arranged by size:
3600 3423 826 601 585 464 407 322
177 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4380 9166 9540 9686 387
Size: 1064 138 2791 4786 374 146 1280
Fragments arranged by size:
4786 2791 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2919 2995 4083 4095 4530 4733
Size: 393 507 780 76 1088 12 435 203
Cuts at: 4733 4774 5091 5163 5668 5867 6057 6525 6790
Size: 41 317 72 505 199 190 468 265
Cuts at: 6790 6936 7032 7063 8642 9239 10026 10294 1239
Size: 146 96 31 1579 597 787 268 1524
Fragments arranged by size:
1579 1524 1088 787 780 597 507 505
468 435 393 317 268 265 203 199
190 146 96 76 72 41 31 12
EcoNI CCTnn'n_nnAGG
Cuts at: 5063 5063
Size: 10579
EcoO109I rG'GnC_Cy
Cuts at: 2144 2336 2730 2882 4507 7540 7728 2144
Size: 192 394 152 1625 3033 188 4995
Fragments arranged by size:
4995 3033 1625 394 192 188 152
EcoRI G'AATT_C
Cuts at: 7888 8266 7888
Size: 378 10201
EcoRV GAT'ATC
Cuts at: 6477 6477
Size: 10579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4832 6062 4832
Size: 1230 9349
FspI TGC'GCA
Cuts at: 9085 9085
Size: 10579
HaeII r_GCGC'y
Cuts at: 1678 9726 1678
Size: 8048 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2843 2875 3062 3094 4286
Size: 32 135 32 1271 32 187 32 1192
Cuts at: 4286 4318 4372 4404 4571 4603 4745 4763 4777
Size: 32 54 32 167 32 142 18 14
Cuts at: 4777 4795 4818 4850 6815 6847 7475 7507 7596
Size: 18 23 32 1965 32 628 32 89
Cuts at: 7596 7628 9220 9252 9294 9326 1373
Size: 32 1592 32 42 32 2626
Fragments arranged by size:
2626 1965 1592 1271 1192 628 187 167
142 135 89 54 42 32 32 32
32 32 32 32 32 32 32 32
32 32 23 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 5500 6812 7194 7230 7923 8169
Size: 988 281 3562 1312 382 36 693 246
Cuts at: 8169 669
Size: 3079
Fragments arranged by size:
3562 3079 1312 988 693 382 281 246
36
HindIII A'AGCT_T
Cuts at: 748 2565 7777 748
Size: 1817 5212 3550
Fragments arranged by size:
5212 3550 1817
HpaI GTT'AAC
Cuts at: 7194 8169 7194
Size: 975 9604
KpnI G_GTAC'C
Cuts at: 7904 7904
Size: 10579
MfeI C'AATT_G
Cuts at: 8178 8178
Size: 10579
MluI A'CGCG_T
Cuts at: 2015 10455 2015
Size: 8440 2139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2000 3200 4849 5070 5295 5470 9693 9877
Size: 695 1200 1649 221 225 175 4223 184
Cuts at: 9877 1305
Size: 2007
Fragments arranged by size:
4223 2007 1649 1200 695 225 221 184
175
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 2140 6737 7565 10
Size: 54 1425 343 180 128 4597 828 3024
Fragments arranged by size:
4597 3024 1425 828 343 180 128 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 3069 5814 5963 7428 8537 8896
Size: 648 1626 277 2745 149 1465 1109 359
Cuts at: 8896 9055 518
Size: 159 2042
Fragments arranged by size:
2745 2042 1626 1465 1109 648 359 277
159 149
MspA1I CmG'CkG
Cuts at: 1606 6251 6558 7505 7995 8672 9820 10065 1606
Size: 4645 307 947 490 677 1148 245 2120
Fragments arranged by size:
4645 2120 1148 947 677 490 307 245
NcoI C'CATG_G
Cuts at: 513 1065 2643 4874 513
Size: 552 1578 2231 6218
Fragments arranged by size:
6218 2231 1578 552
NdeI CA'TA_TG
Cuts at: 387 4648 5504 387
Size: 4261 856 5462
Fragments arranged by size:
5462 4261 856
NheI G'CTAG_C
Cuts at: 1052 5089 1052
Size: 4037 6542
NotI GC'GGCC_GC
Cuts at: 8026 8026
Size: 10579
NspI r_CATG'y
Cuts at: 1132 1229 3902 5968 7345 7937 9482 1132
Size: 97 2673 2066 1377 592 1545 2229
Fragments arranged by size:
2673 2229 2066 1545 1377 592 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10579
PciI A'CATG_T
Cuts at: 7341 9478 7341
Size: 2137 8442
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 10579
PfoI T'CCnGG_A
Cuts at: 5109 5560 7115 5109
Size: 451 1555 8573
Fragments arranged by size:
8573 1555 451
PmeI GTTT'AAAC
Cuts at: 7883 7883
Size: 10579
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8648 8680 10161 10193 8648
Size: 32 1481 32 9034
Fragments arranged by size:
9034 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2144 2336 2882 4507 7540 2144
Size: 192 546 1625 3033 5183
Fragments arranged by size:
5183 3033 1625 546 192
PshAI GACnn'nnGTC
Cuts at: 1613 6131 6807 1613
Size: 4518 676 5385
Fragments arranged by size:
5385 4518 676
PsiI TTA'TAA
Cuts at: 2378 3279 8149 2378
Size: 901 4870 4808
Fragments arranged by size:
4870 4808 901
PspXI vC'TCGA_Gb
Cuts at: 4378 4378
Size: 10579
PstI C_TGCA'G
Cuts at: 830 3007 7813 7931 830
Size: 2177 4806 118 3478
Fragments arranged by size:
4806 3478 2177 118
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10579
PvuII CAG'CTG
Cuts at: 6251 6558 7505 6251
Size: 307 947 9325
Fragments arranged by size:
9325 947 307
SalI G'TCGA_C
Cuts at: 1936 6810 7921 1936
Size: 4874 1111 4594
Fragments arranged by size:
4874 4594 1111
SapI GCTCTTCn'nnn_
Cuts at: 4953 6033 7082 4953
Size: 1080 1049 8450
Fragments arranged by size:
8450 1080 1049
SbfI CC_TGCA'GG
Cuts at: 3007 7931 3007
Size: 4924 5655
ScaI AGT'ACT
Cuts at: 2712 8827 10539 2712
Size: 6115 1712 2752
Fragments arranged by size:
6115 2752 1712
SexAI A'CCwGG_T
Cuts at: 2722 3282 2722
Size: 560 10019
SfcI C'TryA_G
Cuts at: 826 1045 2501 2734 3003 5427 7809 7927 9062
Size: 219 1456 233 269 2424 2382 118 1135
Cuts at: 9062 9743 9934 826
Size: 681 191 1471
Fragments arranged by size:
2424 2382 1471 1456 1135 681 269 233
219 191 118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10579
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10579
SmaI CCC'GGG
Cuts at: 1872 7906 1872
Size: 6034 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 2175 2538 3041 3079 3093
Size: 197 926 111 121 363 503 38 14
Cuts at: 3093 3435 4378 5581 5825 8683 9584 9846 820
Size: 342 943 1203 244 2858 901 262 1553
Fragments arranged by size:
2858 1553 1203 943 926 901 503 363
342 262 244 197 121 111 38 14
SnaBI TAC'GTA
Cuts at: 493 8370 493
Size: 7877 2702
SpeI A'CTAG_T
Cuts at: 152 6164 152
Size: 6012 4567
SphI G_CATG'C
Cuts at: 1132 7937 1132
Size: 6805 3774
SspI AAT'ATT
Cuts at: 5 52 8503 5
Size: 47 8451 2081
Fragments arranged by size:
8451 2081 47
StuI AGG'CCT
Cuts at: 1517 2060 6671 1517
Size: 543 4611 5425
Fragments arranged by size:
5425 4611 543
StyI C'CwwG_G
Cuts at: 513 1065 2643 3635 4803 4874 7710 8018 513
Size: 552 1578 992 1168 71 2836 308 3074
Fragments arranged by size:
3074 2836 1578 1168 992 552 308 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2385 5975 7264 7676 8606 8623 8776
Size: 1255 805 3590 1289 412 930 17 153
Cuts at: 8776 8961 9406 325
Size: 185 445 1498
Fragments arranged by size:
3590 1498 1289 1255 930 805 445 412
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2360 2702 2710
Size: 274 80 33 51 1443 383 342 8
Cuts at: 2710 4920 5976 8825 10537 96
Size: 2210 1056 2849 1712 138
Fragments arranged by size:
2849 2210 1712 1443 1056 383 342 274
138 80 51 33 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4000 4032 5814 5846 10539 10571 4000
Size: 32 1782 32 4693 32 4008
Fragments arranged by size:
4693 4008 1782 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3318 4300 4948 1316
Size: 2002 982 648 6947
Fragments arranged by size:
6947 2002 982 648
XbaI T'CTAG_A
Cuts at: 5761 7915 5761
Size: 2154 8425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3679 4589 6595 3679
Size: 910 2006 7663
Fragments arranged by size:
7663 2006 910
XhoI C'TCGA_G
Cuts at: 1943 4378 1943
Size: 2435 8144
XmaI C'CCGG_G
Cuts at: 1870 7904 1870
Size: 6034 4545
XmnI GAAnn'nnTTC
Cuts at: 2487 3484 3874 4771 8708 2487
Size: 997 390 897 3937 4358
Fragments arranged by size:
4358 3937 997 897 390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 3450 8387 276
Size: 53 83 186 936 1786 130 4937 2468
Fragments arranged by size:
4937 2468 1786 936 186 130 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI