(Circular) (Six-base) MAPSORT of: pFN21AE4131.seq Check: 5927 from: 1 to: 6130 pFN21AE4131 6130 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2160 3473 1937 Size: 223 1313 4594 Fragments arranged by size: 4594 1313 223 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2660 3127 3378 4193 5577 5845 1632 Size: 1028 467 251 815 1384 268 1917 Fragments arranged by size: 1917 1384 1028 815 467 268 251 AfeI AGC'GCT Cuts at: 2145 2145 Size: 6130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 3097 5029 6006 1203 Size: 1894 1932 977 1327 Fragments arranged by size: 1932 1894 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2108 3435 1184 Size: 924 1327 3879 Fragments arranged by size: 3879 1327 924 AlwNI CAG_nnn'CTG Cuts at: 2095 3873 5445 2095 Size: 1778 1572 2780 Fragments arranged by size: 2780 1778 1572 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3124 3439 3665 3817 5926 1493 Size: 1631 315 226 152 2109 1697 Fragments arranged by size: 2109 1697 1631 315 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3455 1870 Size: 73 1512 4545 Fragments arranged by size: 4545 1512 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3451 4906 618 Size: 325 217 627 1664 1455 1842 Fragments arranged by size: 1842 1664 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 3449 1391 Size: 621 1437 4072 Fragments arranged by size: 4072 1437 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2329 928 Size: 971 430 4729 Fragments arranged by size: 4729 971 430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 2902 1548 Size: 1354 4776 BfrBI ATG'CAT Cuts at: 2877 2877 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3322 6125 3322 Size: 2803 3327 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 3043 3241 4191 5347 1790 Size: 1253 198 950 1156 2573 Fragments arranged by size: 2573 1253 1156 950 198 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3861 4819 442 Size: 416 53 83 399 24 2444 958 1753 Fragments arranged by size: 2444 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2059 2117 3224 4790 1239 Size: 820 58 1107 1566 2579 Fragments arranged by size: 2579 1566 1107 820 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2214 4255 5120 5418 2214 Size: 2041 865 298 2926 Fragments arranged by size: 2926 2041 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 2181 6124 2181 Size: 3943 2187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2438 2922 4563 4961 5235 5382 5793 2438 Size: 484 1641 398 274 147 411 2775 Fragments arranged by size: 2775 1641 484 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5905 Fragments arranged by size: 5905 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2425 1186 Size: 1239 4891 BseYI C'CCAG_C Cuts at: 2348 2455 3003 5333 5829 2348 Size: 107 548 2330 496 2649 Fragments arranged by size: 2649 2330 548 496 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5557 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 4191 4276 5347 2012 Size: 2179 85 1071 2795 Fragments arranged by size: 2795 2179 1071 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 3043 3241 3449 4191 4276 5347 Size: 399 222 1031 198 208 742 85 1071 Cuts at: 5347 1391 Size: 2174 Fragments arranged by size: 2174 1071 1031 742 399 222 208 198 85 BspEI T'CCGG_A Cuts at: 2438 5793 2438 Size: 3355 2775 BspHI T'CATG_A Cuts at: 2488 4019 5749 2488 Size: 1531 1730 2869 Fragments arranged by size: 2869 1730 1531 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2127 2515 3322 3460 3827 4212 4229 5681 Size: 155 388 807 138 367 385 17 1452 Cuts at: 5681 5767 5779 6125 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 807 388 385 367 346 155 138 86 17 12 BstZ17I GTA'TAC Cuts at: 2161 2161 Size: 6130 Bsu36I CC'TnA_GG Cuts at: 2342 2342 Size: 6130 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5578 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2854 487 Size: 683 56 1628 3763 Fragments arranged by size: 3763 1628 683 56 BtsI GCAGTG_nn' Cuts at: 3646 4440 4460 3646 Size: 794 20 5316 Fragments arranged by size: 5316 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 2629 2718 3434 2629 Size: 89 716 5325 Fragments arranged by size: 5325 716 89 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2962 3577 4466 Size: 54 1425 50 275 18 1132 615 889 Cuts at: 4466 6095 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1132 889 615 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3060 4060 1353 Size: 1707 1000 3423 Fragments arranged by size: 3423 1707 1000 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2117 2660 3127 3224 3378 4193 Size: 393 427 58 543 467 97 154 815 Cuts at: 4193 4790 5577 5845 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 815 787 597 543 467 427 393 268 154 97 58 EcoO109I rG'GnC_Cy Cuts at: 2285 2285 Size: 6130 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FspI TGC'GCA Cuts at: 3348 4636 3348 Size: 1288 4842 HaeII r_GCGC'y Cuts at: 1678 2147 5277 1678 Size: 469 3130 2531 Fragments arranged by size: 3130 2531 469 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3307 3339 4771 4803 4845 Size: 32 135 32 1735 32 1432 32 42 Cuts at: 4845 4877 1373 Size: 32 2626 Fragments arranged by size: 2626 1735 1432 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3160 3474 3720 669 Size: 988 281 1222 314 246 3079 Fragments arranged by size: 3079 1222 988 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5244 5428 1305 Size: 3939 184 2007 Fragments arranged by size: 3939 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2493 3276 4088 4447 4606 518 Size: 648 924 403 783 812 359 159 2042 Fragments arranged by size: 2042 924 812 783 648 403 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 3074 3546 4223 5371 5616 1606 Size: 420 1048 472 677 1148 245 2120 Fragments arranged by size: 2120 1148 1048 677 472 420 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5578 NdeI CA'TA_TG Cuts at: 387 3273 387 Size: 2886 3244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NsiI A_TGCA'T Cuts at: 2879 2879 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2877 3101 3488 5033 1132 Size: 97 1648 224 387 1545 2229 Fragments arranged by size: 2229 1648 1545 387 224 97 PacI TTA_AT'TAA Cuts at: 3399 3399 Size: 6130 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 3097 5029 3097 Size: 1932 4198 PflMI CCAn_nnn'nTGG Cuts at: 1108 2243 1108 Size: 1135 4995 PfoI T'CCnGG_A Cuts at: 2749 2749 Size: 6130 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 2958 3700 2958 Size: 742 5388 PstI C_TGCA'G Cuts at: 830 2946 3330 3482 830 Size: 2116 384 152 3478 Fragments arranged by size: 3478 2116 384 152 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 3074 3074 Size: 6130 SacI G_AGCT'C Cuts at: 2012 2012 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2223 2590 2942 3326 3478 4613 5294 Size: 219 1178 367 352 384 152 1135 681 Cuts at: 5294 5485 826 Size: 191 1471 Fragments arranged by size: 1471 1178 1135 681 384 367 352 219 191 152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2229 4234 5135 5397 820 Size: 197 926 286 2005 901 262 1553 Fragments arranged by size: 2005 1553 926 901 286 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 2877 3488 1132 Size: 1745 611 3774 Fragments arranged by size: 3774 1745 611 SspI AAT'ATT Cuts at: 5 52 2825 4054 5 Size: 47 2773 1229 2081 Fragments arranged by size: 2773 2081 1229 47 StuI AGG'CCT Cuts at: 1517 3182 1517 Size: 1665 4465 StyI C'CwwG_G Cuts at: 513 1065 3569 513 Size: 552 2504 3074 Fragments arranged by size: 3074 2504 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2413 4157 4174 4327 4512 4957 325 Size: 1255 833 1744 17 153 185 445 1498 Fragments arranged by size: 1744 1498 1255 833 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 2569 2670 Size: 274 80 33 51 1443 570 22 101 Cuts at: 2670 4376 6088 96 Size: 1706 1712 138 Fragments arranged by size: 1712 1706 1443 570 274 138 101 80 51 33 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2546 2578 6090 6122 2546 Size: 32 3512 32 2554 Fragments arranged by size: 3512 2554 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI