Restriction map for
pFN21AB6538
(Linear) (Six-base) MAPSORT of: KIBB6538.seq Check: 1138 from: 1 to: 1173
KIBB6538.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1173
Size: 512 661
AhdI GACnn_n'nnGTC
Cuts at: 0 387 1173
Size: 387 786
AlwNI CAG_nnn'CTG
Cuts at: 0 207 618 822 1057 1173
Size: 207 411 204 235 116
Fragments arranged by size:
411 235 207 204 116
ApaI G_GGCC'C
Cuts at: 0 1135 1173
Size: 1135 38
ApoI r'AATT_y
Cuts at: 0 661 1173
Size: 661 512
BanII G_rGCy'C
Cuts at: 0 80 107 674 829 955 1009 1046 1135
Size: 80 27 567 155 126 54 37 89
Cuts at: 1135 1173
Size: 38
Fragments arranged by size:
567 155 126 89 80 54 38 37
27
BbsI GAAGACnn'nnnn_
Cuts at: 0 240 280 364 396 1160 1173
Size: 240 40 84 32 764 13
Fragments arranged by size:
764 240 84 40 32 13
BbvCI CC'TCA_GC
Cuts at: 0 854 1173
Size: 854 319
BlpI GC'TnA_GC
Cuts at: 0 1040 1173
Size: 1040 133
Bme1580I G_kGCm'C
Cuts at: 0 424 1135 1173
Size: 424 711 38
Fragments arranged by size:
711 424 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1040 1173
Size: 1040 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 710 844 1173
Size: 289 421 134 329
Fragments arranged by size:
421 329 289 134
Bpu10I CC'TnA_GC
Cuts at: 0 676 854 1173
Size: 676 178 319
Fragments arranged by size:
676 319 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1173
Size: 640 533
BsaWI w'CCGG_w
Cuts at: 0 113 1173
Size: 113 1060
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 796 811 826 841 1173
Size: 91 30 675 15 15 15 332
Fragments arranged by size:
675 332 91 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 117 562 565 616 1039 1173
Size: 117 445 3 51 423 134
Fragments arranged by size:
445 423 134 117 51 3
BseYI C'CCAG_C
Cuts at: 0 411 441 501 992 1135 1173
Size: 411 30 60 491 143 38
Fragments arranged by size:
491 411 143 60 38 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1173
Size: 467 706
BsiHKAI G_wGCw'C
Cuts at: 0 38 80 1173
Size: 38 42 1093
Fragments arranged by size:
1093 42 38
BsmI GAATG_Cn'
Cuts at: 0 710 1173
Size: 710 463
Bsp1286I G_dGCh'C
Cuts at: 0 38 80 107 424 674 829 955 1009
Size: 38 42 27 317 250 155 126 54
Cuts at: 1009 1046 1135 1173
Size: 37 89 38
Fragments arranged by size:
317 250 155 126 89 54 42 38
38 37 27
BspEI T'CCGG_A
Cuts at: 0 113 1173
Size: 113 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 1173
Size: 215 958
BsrBI CCG'CTC
Cuts at: 0 134 165 254 1085 1173
Size: 134 31 89 831 88
Fragments arranged by size:
831 134 89 88 31
BsrDI GCAATG_nn'
Cuts at: 0 336 951 1173
Size: 336 615 222
Fragments arranged by size:
615 336 222
BssSI C'ACGA_G
Cuts at: 0 417 544 1173
Size: 417 127 629
Fragments arranged by size:
629 417 127
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 1173
Size: 576 597
BstYI r'GATC_y
Cuts at: 0 221 1173
Size: 221 952
BtgI C'CryG_G
Cuts at: 0 513 575 926 1173
Size: 513 62 351 247
Fragments arranged by size:
513 351 247 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 277 1173
Size: 277 896
BtsI GCAGTG_nn'
Cuts at: 0 563 1173
Size: 563 610
EaeI y'GGCC_r
Cuts at: 0 306 510 516 638 771 785 1173
Size: 306 204 6 122 133 14 388
Fragments arranged by size:
388 306 204 133 122 14 6
EarI CTCTTCn'nnn_
Cuts at: 0 172 1173
Size: 172 1001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1165 1173
Size: 1165 8
EcoICRI GAG'CTC
Cuts at: 0 78 1173
Size: 78 1095
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 512 710 844 1173
Size: 289 223 198 134 329
Fragments arranged by size:
329 289 223 198 134
EcoNI CCTnn'n_nnAGG
Cuts at: 0 598 1173
Size: 598 575
EcoO109I rG'GnC_Cy
Cuts at: 0 204 819 1131 1132 1173
Size: 204 615 312 1 41
Fragments arranged by size:
615 312 204 41 1
EcoRI G'AATT_C
Cuts at: 0 661 1173
Size: 661 512
FspI TGC'GCA
Cuts at: 0 535 1173
Size: 535 638
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 373 400 405 432 790 796
Size: 91 32 250 27 5 27 358 6
Cuts at: 796 811 822 828 843 895 927 996 1028
Size: 15 11 6 15 52 32 69 32
Cuts at: 1028 1173
Size: 145
Fragments arranged by size:
358 250 145 91 69 52 32 32
32 27 27 15 15 11 6 6
5
MscI TGG'CCA
Cuts at: 0 640 787 1173
Size: 640 147 386
Fragments arranged by size:
640 386 147
MslI CAynn'nnrTG
Cuts at: 0 719 1173
Size: 719 454
MspA1I CmG'CkG
Cuts at: 0 333 618 1173
Size: 333 285 555
Fragments arranged by size:
555 333 285
NcoI C'CATG_G
Cuts at: 0 575 926 1173
Size: 575 351 247
Fragments arranged by size:
575 351 247
NspI r_CATG'y
Cuts at: 0 432 1173
Size: 432 741
PasI CC'CwG_GG
Cuts at: 0 1128 1173
Size: 1128 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 822 1173
Size: 822 351
PfoI T'CCnGG_A
Cuts at: 0 377 1173
Size: 377 796
PpuMI rG'GwC_Cy
Cuts at: 0 204 819 1173
Size: 204 615 354
Fragments arranged by size:
615 354 204
PspOMI G'GGCC_C
Cuts at: 0 1131 1173
Size: 1131 42
PstI C_TGCA'G
Cuts at: 0 398 623 1173
Size: 398 225 550
Fragments arranged by size:
550 398 225
PvuII CAG'CTG
Cuts at: 0 333 618 1173
Size: 333 285 555
Fragments arranged by size:
555 333 285
SacI G_AGCT'C
Cuts at: 0 80 1173
Size: 80 1093
SapI GCTCTTCn'nnn_
Cuts at: 0 172 1173
Size: 172 1001
SbfI CC_TGCA'GG
Cuts at: 0 398 1173
Size: 398 775
SfcI C'TryA_G
Cuts at: 0 394 619 938 1173
Size: 394 225 319 235
Fragments arranged by size:
394 319 235 225
SmlI C'TyrA_G
Cuts at: 0 655 1173
Size: 655 518
StyI C'CwwG_G
Cuts at: 0 92 575 594 926 1173
Size: 92 483 19 332 247
Fragments arranged by size:
483 332 247 92 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1086 1173
Size: 1086 87
TatI w'GTAC_w
Cuts at: 0 1048 1173
Size: 1048 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 43 75 355 387 1173
Size: 43 32 280 32 786
Fragments arranged by size:
786 280 43 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1143 1173
Size: 1143 30
XmnI GAAnn'nnTTC
Cuts at: 0 235 1173
Size: 235 938
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApoI BanII BbsI BbvCI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BssSI BstXI BstYI BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI Hin4I MscI MslI MspA1I NcoI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI
SbfI SfcI SmlI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoRV FalI FseI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI ZraI