(Circular) (Six-base) MAPSORT of: pFN21AE9610.seq Check: 8442 from: 1 to: 5602 pFN21AE9610 5602 bp With 182 enzymes: SgfI * March 10, 2017 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2142 2945 1937 Size: 205 803 4594 Fragments arranged by size: 4594 803 205 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3665 5049 5317 1632 Size: 2033 1384 268 1917 Fragments arranged by size: 2033 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 4501 5478 1203 Size: 3298 977 1327 Fragments arranged by size: 3298 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2669 2701 2669 Size: 32 5570 AlwNI CAG_nnn'CTG Cuts at: 2369 2544 3345 4917 2369 Size: 175 801 1572 3054 Fragments arranged by size: 3054 1572 801 175 ApaI G_GGCC'C Cuts at: 2123 2135 2123 Size: 12 5590 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2911 3137 3289 5398 1493 Size: 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2477 2559 2927 1870 Size: 73 534 82 368 4545 Fragments arranged by size: 4545 534 368 82 73 BamHI G'GATC_C Cuts at: 2932 3299 2932 Size: 367 5235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2923 4378 618 Size: 325 217 627 1136 1455 1842 Fragments arranged by size: 1842 1455 1136 627 325 217 BanII G_rGCy'C Cuts at: 1391 2123 2135 2921 1391 Size: 732 12 786 4072 Fragments arranged by size: 4072 786 732 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3793 3827 1903 Size: 34 1856 34 3678 Fragments arranged by size: 3678 1856 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2605 3494 4704 2605 Size: 889 1210 3503 Fragments arranged by size: 3503 1210 889 BclI T'GATC_A Cuts at: 1548 1548 Size: 5602 BfrBI ATG'CAT Cuts at: 2387 2387 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2555 4213 136 Size: 107 122 71 631 605 883 1658 1525 Fragments arranged by size: 1658 1525 883 631 605 122 107 71 BglII A'GATC_T Cuts at: 2179 5597 2179 Size: 3418 2184 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 2067 2123 2135 3663 4819 1790 Size: 277 56 12 1528 1156 2573 Fragments arranged by size: 2573 1528 1156 277 56 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2379 2692 3333 Size: 416 53 83 399 24 962 313 641 Cuts at: 3333 4291 442 Size: 958 1753 Fragments arranged by size: 1753 962 958 641 416 399 313 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2204 2759 2903 4262 1239 Size: 803 162 555 144 1359 2579 Fragments arranged by size: 2579 1359 803 555 162 144 Bpu10I CC'TnA_GC Cuts at: 2626 2626 Size: 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2674 3393 493 Size: 711 1470 719 2702 Fragments arranged by size: 2702 1470 719 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 3409 3791 4426 Size: 53 83 186 936 567 1309 382 635 Cuts at: 4426 275 Size: 1451 Fragments arranged by size: 1451 1309 936 635 567 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2706 4035 4433 4707 4854 5265 2706 Size: 1329 398 274 147 411 3043 Fragments arranged by size: 3043 1329 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2330 2360 2669 2699 1178 Size: 30 165 30 927 30 309 30 4081 Fragments arranged by size: 4081 927 309 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5602 BseYI C'CCAG_C Cuts at: 2128 2274 4805 5301 2128 Size: 146 2531 496 2429 Fragments arranged by size: 2531 2429 496 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5029 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2673 3663 3748 4819 2673 Size: 990 85 1071 3456 Fragments arranged by size: 3456 1071 990 85 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2123 2135 2673 2921 3663 3748 Size: 399 277 56 12 538 248 742 85 Cuts at: 3748 4819 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 277 248 85 56 12 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 844 Size: 2113 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2230 3489 1394 Size: 429 407 1259 3507 Fragments arranged by size: 3507 1259 429 407 BsrDI GCAATG_nn' Cuts at: 65 1248 4097 4271 65 Size: 1183 2849 174 1396 Fragments arranged by size: 2849 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2008 2179 2932 3299 3684 3701 5153 5239 Size: 36 171 753 367 385 17 1452 86 Cuts at: 5239 5251 5597 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 753 385 367 346 171 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2067 2136 513 Size: 552 939 63 69 3979 Fragments arranged by size: 3979 939 552 69 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 3118 3912 3932 3118 Size: 794 20 4788 Fragments arranged by size: 4788 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3049 3938 5567 Size: 54 1425 50 275 18 1219 889 1629 Cuts at: 5567 8 Size: 43 Fragments arranged by size: 1629 1425 1219 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3532 1353 Size: 2179 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2765 4189 4563 4709 387 Size: 1064 138 1176 1424 374 146 1280 Fragments arranged by size: 1424 1280 1176 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2204 2759 2903 3665 4262 5049 Size: 393 410 162 555 144 762 597 787 Cuts at: 5049 5317 1239 Size: 268 1524 Fragments arranged by size: 1524 787 762 597 555 410 393 268 162 144 EcoO109I rG'GnC_Cy Cuts at: 2119 2120 2132 2832 2119 Size: 1 12 700 4889 Fragments arranged by size: 4889 700 12 1 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 2192 2282 4749 1678 Size: 514 90 2467 2531 Fragments arranged by size: 2531 2467 514 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2609 2641 2762 Size: 32 135 32 704 32 301 32 121 Cuts at: 2762 2794 4243 4275 4317 4349 1373 Size: 32 1449 32 42 32 2626 Fragments arranged by size: 2626 1449 704 301 135 121 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3192 669 Size: 988 281 1008 246 3079 Fragments arranged by size: 3079 1008 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4716 4900 1305 Size: 3411 184 2007 Fragments arranged by size: 3411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3780 Fragments arranged by size: 3780 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3560 3919 4078 518 Size: 648 2394 359 159 2042 Fragments arranged by size: 2394 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2069 2715 3018 3695 4843 5088 1606 Size: 437 26 646 303 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 646 437 303 245 26 NcoI C'CATG_G Cuts at: 513 1065 2004 2136 513 Size: 552 939 132 3979 Fragments arranged by size: 3979 939 552 132 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NsiI A_TGCA'T Cuts at: 2389 2389 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2320 2960 4505 1132 Size: 97 1091 640 1545 2229 Fragments arranged by size: 2229 1545 1091 640 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5602 PfoI T'CCnGG_A Cuts at: 2736 2868 2736 Size: 132 5470 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PmlI CAC'GTG Cuts at: 2674 2674 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2669 2701 3671 3703 5184 5216 2669 Size: 32 970 32 1481 32 3055 Fragments arranged by size: 3055 1481 970 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2832 2832 Size: 5602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PspOMI G'GGCC_C Cuts at: 2119 2131 2119 Size: 12 5590 PstI C_TGCA'G Cuts at: 830 2466 2604 2954 830 Size: 1636 138 350 3478 Fragments arranged by size: 3478 1636 350 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 PvuII CAG'CTG Cuts at: 2715 2715 Size: 5602 SacII CC_GC'GG Cuts at: 2070 2070 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SanDI GG'GwC_CC Cuts at: 2832 2832 Size: 5602 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SfcI C'TryA_G Cuts at: 826 1045 2462 2600 2950 4085 4766 4957 826 Size: 219 1417 138 350 1135 681 191 1471 Fragments arranged by size: 1471 1417 1135 681 350 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2479 2561 2929 1872 Size: 607 82 368 4545 Fragments arranged by size: 4545 607 368 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 3706 4607 4869 820 Size: 197 926 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2320 2960 1132 Size: 1188 640 3774 Fragments arranged by size: 3774 1188 640 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2004 2136 2550 3041 513 Size: 552 939 132 414 491 3074 Fragments arranged by size: 3074 939 552 491 414 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2426 3629 3646 3799 3984 4429 325 Size: 1255 846 1203 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1203 846 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3848 5560 96 Size: 274 80 33 51 1443 1871 1712 138 Fragments arranged by size: 1871 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 2267 1316 Size: 951 4651 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2477 2559 2927 1870 Size: 607 82 368 4545 Fragments arranged by size: 4545 607 368 82 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI