(Circular) (Six-base) MAPSORT of: pFN21AE9256.seq Check: 7432 from: 1 to: 6859 pFN21AE9256 6859 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2817 3814 4092 2817 Size: 997 278 5584 Fragments arranged by size: 5584 997 278 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4669 278 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2304 4202 1937 Size: 367 1898 4594 Fragments arranged by size: 4594 1898 367 Acc65I G'GTAC_C Cuts at: 4180 4180 Size: 6859 AclI AA'CG_TT Cuts at: 1563 4986 5359 1563 Size: 3423 373 3063 Fragments arranged by size: 3423 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2510 2801 3455 3464 3836 3863 4123 Size: 695 183 291 654 9 372 27 260 Cuts at: 4123 4922 6306 6574 1632 Size: 799 1384 268 1917 Fragments arranged by size: 1917 1384 799 695 654 372 291 268 260 183 27 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6662 AflIII A'CryG_T Cuts at: 1203 5758 6735 1203 Size: 4555 977 1327 Fragments arranged by size: 4555 1327 977 AgeI A'CCGG_T Cuts at: 5690 5690 Size: 6859 AhdI GACnn_n'nnGTC Cuts at: 5588 5588 Size: 6859 AleI CACnn'nnGTG Cuts at: 1166 3656 1166 Size: 2490 4369 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2453 1184 Size: 1269 5590 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3459 3491 4031 4063 3459 Size: 32 540 32 6255 Fragments arranged by size: 6255 540 32 32 AlwNI CAG_nnn'CTG Cuts at: 2572 2698 3109 3382 3673 4036 4602 6174 2572 Size: 126 411 273 291 363 566 1572 3257 Fragments arranged by size: 3257 1572 566 411 363 291 273 126 ApaLI G'TGCA_C Cuts at: 3338 4079 4916 6072 3338 Size: 741 837 1156 4125 Fragments arranged by size: 4125 1156 837 741 ApoI r'AATT_y Cuts at: 1493 4168 4394 4546 6655 1493 Size: 2675 226 152 2109 1697 Fragments arranged by size: 2675 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5413 160 Size: 872 4381 1606 Fragments arranged by size: 4381 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 AvaI C'yCGr_G Cuts at: 1870 1943 2247 3081 4015 4130 4184 1870 Size: 73 304 834 934 115 54 4545 Fragments arranged by size: 4545 934 834 304 115 73 54 AvrII C'CTAG_G Cuts at: 2762 2762 Size: 6859 BamHI G'GATC_C Cuts at: 4189 4556 4189 Size: 367 6492 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2877 3749 4089 4180 5635 Size: 325 217 627 1090 872 340 91 1455 Cuts at: 5635 618 Size: 1842 Fragments arranged by size: 1842 1455 1090 872 627 340 325 217 91 BanII G_rGCy'C Cuts at: 1391 2248 2322 3123 3595 3726 4071 4137 4178 Size: 857 74 801 472 131 345 66 41 Cuts at: 4178 1391 Size: 4072 Fragments arranged by size: 4072 857 801 472 345 131 74 66 41 BbeI G_GCGC'C Cuts at: 2881 4093 2881 Size: 1212 5647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2016 3006 928 Size: 971 117 990 4781 Fragments arranged by size: 4781 990 971 117 BbvCI CC'TCA_GC Cuts at: 3318 4115 3318 Size: 797 6062 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5050 5084 1903 Size: 34 3113 34 3678 Fragments arranged by size: 3678 3113 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4751 5961 4751 Size: 1210 5649 BclI T'GATC_A Cuts at: 1548 1548 Size: 6859 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5470 136 Size: 107 122 71 631 605 3798 1525 Fragments arranged by size: 3798 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2048 6854 2048 Size: 4806 2053 BlpI GC'TnA_GC Cuts at: 3366 4276 3366 Size: 910 5949 Bme1580I G_kGCm'C Cuts at: 1790 3342 4083 4920 6076 1790 Size: 1552 741 837 1156 2573 Fragments arranged by size: 2573 1552 1156 837 741 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6859 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4590 5548 442 Size: 416 53 83 399 24 3173 958 1753 Fragments arranged by size: 3173 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6859 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 6859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2279 2408 2573 2621 2708 2825 2939 Size: 938 102 129 165 48 87 117 114 Cuts at: 2939 2979 3110 3179 3404 3443 3469 3569 3590 Size: 40 131 69 225 39 26 100 21 Cuts at: 3590 3602 4058 5519 1239 Size: 12 456 1461 2579 Fragments arranged by size: 2579 1461 938 456 225 165 131 129 117 114 102 100 87 69 48 40 39 26 21 12 Bpu10I CC'TnA_GC Cuts at: 2981 3318 3506 4115 2981 Size: 337 188 609 5725 Fragments arranged by size: 5725 609 337 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3788 3842 4984 5849 6147 3788 Size: 54 1142 865 298 4500 Fragments arranged by size: 4500 1142 865 298 54 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2468 2819 3161 5522 882 Size: 617 969 351 342 2361 2219 Fragments arranged by size: 2361 2219 969 617 351 342 BsaAI yAC'GTr Cuts at: 493 1204 4650 493 Size: 711 3446 2702 Fragments arranged by size: 3446 2702 711 BsaBI GATnn'nnATC Cuts at: 3929 6853 3929 Size: 2924 3935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2878 4090 4666 5048 Size: 53 83 186 936 1345 1212 576 382 Cuts at: 5048 5683 275 Size: 635 1451 Fragments arranged by size: 1451 1345 1212 936 635 576 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5292 5690 5964 6111 6522 5292 Size: 398 274 147 411 5629 Fragments arranged by size: 5629 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3738 3768 3932 3962 4031 Size: 30 165 30 2335 30 164 30 69 Cuts at: 4031 4061 1178 Size: 30 3976 Fragments arranged by size: 3976 2335 165 164 69 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2381 2810 3131 3164 3401 3506 3536 3566 Size: 1195 429 321 33 237 105 30 30 Cuts at: 3566 3759 1186 Size: 193 4286 Fragments arranged by size: 4286 1195 429 321 237 193 105 33 30 30 BseYI C'CCAG_C Cuts at: 2872 2952 3291 3391 3757 6062 6558 2872 Size: 80 339 100 366 2305 496 3173 Fragments arranged by size: 3173 2305 496 366 339 100 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3350 3617 3659 1163 Size: 573 1614 267 42 4363 Fragments arranged by size: 4363 1614 573 267 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2308 3279 4309 5070 6098 1540 Size: 275 186 307 971 1030 761 1028 2301 Fragments arranged by size: 2301 1030 1028 971 761 307 275 186 BsiHKAI G_wGCw'C Cuts at: 2322 3123 3342 3595 3978 4071 4083 4137 4920 Size: 801 219 253 383 93 12 54 783 Cuts at: 4920 5005 6076 2322 Size: 85 1071 3105 Fragments arranged by size: 3105 1071 801 783 383 253 219 93 85 54 12 BsmI GAATG_Cn' Cuts at: 4370 4463 4370 Size: 93 6766 BsmBI CGTCTCn'nnnn_ Cuts at: 2891 2891 Size: 6859 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2248 2322 3123 3342 3595 3726 3978 Size: 399 458 74 801 219 253 131 252 Cuts at: 3978 4071 4083 4137 4178 4920 5005 6076 1391 Size: 93 12 54 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 801 742 458 399 253 252 219 131 93 85 74 54 41 12 BspEI T'CCGG_A Cuts at: 6522 6522 Size: 6859 BspHI T'CATG_A Cuts at: 4748 6478 4748 Size: 1730 5129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2598 2817 3118 3814 4092 4214 844 Size: 1754 219 301 696 278 122 3489 Fragments arranged by size: 3489 1754 696 301 278 219 122 BsrBI CCG'CTC Cuts at: 1394 1823 2209 2350 3226 3538 3586 4746 1394 Size: 429 386 141 876 312 48 1160 3507 Fragments arranged by size: 3507 1160 876 429 386 312 141 48 BsrDI GCAATG_nn' Cuts at: 65 1248 5354 5528 65 Size: 1183 4106 174 1396 Fragments arranged by size: 4106 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3914 3951 5503 5690 1700 Size: 110 2104 37 1552 187 2869 Fragments arranged by size: 2869 2104 1552 187 110 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6859 BssHII G'CGCG_C Cuts at: 1925 2802 3730 1925 Size: 877 928 5054 Fragments arranged by size: 5054 928 877 BssSI C'ACGA_G Cuts at: 3173 3677 4919 5931 3173 Size: 504 1242 1012 4101 Fragments arranged by size: 4101 1242 1012 504 BstAPI GCAn_nnn'nTGC Cuts at: 794 2149 2572 3109 3337 3673 3900 794 Size: 1355 423 537 228 336 227 3753 Fragments arranged by size: 3753 1355 537 423 336 228 227 BstBI TT'CG_AA Cuts at: 2183 5663 2183 Size: 3480 3379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6859 BstXI CCAn_nnnn'nTGG Cuts at: 3016 3658 3805 3016 Size: 642 147 6070 Fragments arranged by size: 6070 642 147 BstYI r'GATC_y Cuts at: 1972 2048 2175 2499 2683 3879 4189 4556 4941 Size: 76 127 324 184 1196 310 367 385 Cuts at: 4941 4958 6410 6496 6508 6854 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1196 385 367 346 324 310 184 127 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2973 3735 3936 4059 4152 Size: 552 939 24 945 762 201 123 93 Cuts at: 4152 513 Size: 3220 Fragments arranged by size: 3220 945 939 762 552 201 123 93 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6120 Fragments arranged by size: 6120 683 56 BtsI GCAGTG_nn' Cuts at: 2408 2519 4375 5169 5189 2408 Size: 111 1856 794 20 4078 Fragments arranged by size: 4078 1856 794 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6824 DraI TTT'AAA Cuts at: 4163 4163 Size: 6859 DraIII CAC_nnn'GTG Cuts at: 4060 4060 Size: 6859 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5866 809 Size: 507 4550 1802 Fragments arranged by size: 4550 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2937 3136 3276 Size: 54 1425 50 275 18 1107 199 140 Cuts at: 3276 3660 4306 5195 6824 8 Size: 384 646 889 1629 43 Fragments arranged by size: 1629 1425 1107 889 646 384 275 199 140 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3276 4306 1537 Size: 275 1464 1030 4090 Fragments arranged by size: 4090 1464 1030 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2841 4142 4789 1353 Size: 1488 1301 647 3423 Fragments arranged by size: 3423 1488 1301 647 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2378 3512 3716 5446 5820 5966 Size: 1064 138 789 1134 204 1730 374 146 Cuts at: 5966 387 Size: 1280 Fragments arranged by size: 1730 1280 1134 1064 789 374 204 146 138 EcoICRI GAG'CTC Cuts at: 2320 3121 3593 4069 4135 2320 Size: 801 472 476 66 5044 Fragments arranged by size: 5044 801 476 472 66 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2279 2327 2408 2510 2573 2621 Size: 393 545 102 48 81 102 63 48 Cuts at: 2621 2708 2801 2825 2939 2979 3110 3179 3404 Size: 87 93 24 114 40 131 69 225 Cuts at: 3404 3443 3455 3464 3469 3569 3590 3602 3836 Size: 39 12 9 5 100 21 12 234 Cuts at: 3836 3863 4058 4123 4922 5519 6306 6574 1239 Size: 27 195 65 799 597 787 268 1524 Fragments arranged by size: 1524 799 787 597 545 393 268 234 225 195 131 114 102 102 100 93 87 81 69 65 63 48 48 40 39 27 24 21 12 12 9 5 EcoNI CCTnn'n_nnAGG Cuts at: 2198 3677 2198 Size: 1479 5380 EcoO109I rG'GnC_Cy Cuts at: 3205 3252 3379 3205 Size: 47 127 6685 Fragments arranged by size: 6685 127 47 EcoRI G'AATT_C Cuts at: 4168 4546 4168 Size: 378 6481 FspI TGC'GCA Cuts at: 5365 5365 Size: 6859 HaeII r_GCGC'y Cuts at: 1678 2551 2881 3066 3081 3182 3354 3673 4093 Size: 873 330 185 15 101 172 319 420 Cuts at: 4093 6006 1678 Size: 1913 2531 Fragments arranged by size: 2531 1913 873 420 330 319 185 172 101 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2668 2700 3349 3381 3738 Size: 32 135 32 1096 32 649 32 357 Cuts at: 3738 3770 5500 5532 5574 5606 1373 Size: 32 1730 32 42 32 2626 Fragments arranged by size: 2626 1730 1096 649 357 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2305 4203 4449 669 Size: 988 281 367 1898 246 3079 Fragments arranged by size: 3079 1898 988 367 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6859 HpaI GTT'AAC Cuts at: 4449 4449 Size: 6859 KasI G'GCGC_C Cuts at: 2877 4089 2877 Size: 1212 5647 KpnI G_GTAC'C Cuts at: 4184 4184 Size: 6859 MfeI C'AATT_G Cuts at: 4458 4458 Size: 6859 MluI A'CGCG_T Cuts at: 6735 6735 Size: 6859 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2538 3126 5973 6157 1305 Size: 1233 588 2847 184 2007 Fragments arranged by size: 2847 2007 1233 588 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2939 3662 10 Size: 54 1425 343 1107 723 3207 Fragments arranged by size: 3207 1425 1107 723 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 3656 4076 4817 5176 5335 518 Size: 648 1035 1455 420 741 359 159 2042 Fragments arranged by size: 2042 1455 1035 741 648 420 359 159 MspA1I CmG'CkG Cuts at: 1606 2155 2386 2584 2788 3109 3135 3142 3295 Size: 549 231 198 204 321 26 7 153 Cuts at: 3295 3370 3580 3694 3853 4275 4952 6100 6345 Size: 75 210 114 159 422 677 1148 245 Cuts at: 6345 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 549 422 321 245 231 210 204 198 159 153 114 75 26 7 NarI GG'CG_CC Cuts at: 2878 4090 2878 Size: 1212 5647 NcoI C'CATG_G Cuts at: 513 1065 2004 2028 2973 3735 513 Size: 552 939 24 945 762 3637 Fragments arranged by size: 3637 945 939 762 552 24 NdeI CA'TA_TG Cuts at: 387 387 Size: 6859 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6859 NotI GC'GGCC_GC Cuts at: 4306 4306 Size: 6859 NspI r_CATG'y Cuts at: 1132 1229 2152 3310 3421 4217 5762 1132 Size: 97 923 1158 111 796 1545 2229 Fragments arranged by size: 2229 1545 1158 923 796 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6859 PciI A'CATG_T Cuts at: 5758 5758 Size: 6859 PflMI CCAn_nnn'nTGG Cuts at: 1108 2974 3145 4153 1108 Size: 1866 171 1008 3814 Fragments arranged by size: 3814 1866 1008 171 PfoI T'CCnGG_A Cuts at: 2628 2841 3882 2628 Size: 213 1041 5605 Fragments arranged by size: 5605 1041 213 PmeI GTTT'AAAC Cuts at: 4163 4163 Size: 6859 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 4928 4960 6441 6473 4031 Size: 32 865 32 1481 32 4417 Fragments arranged by size: 4417 1481 865 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3205 3252 3205 Size: 47 6812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6859 PsiI TTA'TAA Cuts at: 4429 4429 Size: 6859 PspXI vC'TCGA_Gb Cuts at: 2247 4130 2247 Size: 1883 4976 PstI C_TGCA'G Cuts at: 830 2130 2514 2577 2865 3030 3114 3600 3699 Size: 1300 384 63 288 165 84 486 99 Cuts at: 3699 4211 830 Size: 512 3478 Fragments arranged by size: 3478 1300 512 486 384 288 165 99 84 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6859 PvuII CAG'CTG Cuts at: 2155 2386 2584 2788 3109 3142 3295 3370 3580 Size: 231 198 204 321 33 153 75 210 Cuts at: 3580 3694 2155 Size: 114 5320 Fragments arranged by size: 5320 321 231 210 204 198 153 114 75 33 SacI G_AGCT'C Cuts at: 2322 3123 3595 4071 4137 2322 Size: 801 472 476 66 5044 Fragments arranged by size: 5044 801 476 472 66 SalI G'TCGA_C Cuts at: 1936 2303 4201 1936 Size: 367 1898 4594 Fragments arranged by size: 4594 1898 367 SapI GCTCTTCn'nnn_ Cuts at: 2841 4142 2841 Size: 1301 5558 SbfI CC_TGCA'GG Cuts at: 4211 4211 Size: 6859 ScaI AGT'ACT Cuts at: 3284 5107 6819 3284 Size: 1823 1712 3324 Fragments arranged by size: 3324 1823 1712 SfcI C'TryA_G Cuts at: 826 1045 2126 2510 2573 2861 3026 3110 3596 Size: 219 1081 384 63 288 165 84 486 Cuts at: 3596 3695 4207 5342 6023 6214 826 Size: 99 512 1135 681 191 1471 Fragments arranged by size: 1471 1135 1081 681 512 486 384 288 219 191 165 99 84 63 SfoI GGC'GCC Cuts at: 2879 4091 2879 Size: 1212 5647 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6859 SmaI CCC'GGG Cuts at: 1872 3083 4186 1872 Size: 1211 1103 4545 Fragments arranged by size: 4545 1211 1103 SmlI C'TyrA_G Cuts at: 820 1017 1943 2247 3767 3857 4130 4963 5864 Size: 197 926 304 1520 90 273 833 901 Cuts at: 5864 6126 820 Size: 262 1553 Fragments arranged by size: 1553 1520 926 901 833 304 273 262 197 90 SnaBI TAC'GTA Cuts at: 493 4650 493 Size: 4157 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6859 SphI G_CATG'C Cuts at: 1132 2152 3310 4217 1132 Size: 1020 1158 907 3774 Fragments arranged by size: 3774 1158 1020 907 SspI AAT'ATT Cuts at: 5 52 4783 5 Size: 47 4731 2081 Fragments arranged by size: 4731 2081 47 StuI AGG'CCT Cuts at: 1517 3899 1517 Size: 2382 4477 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2762 2973 3219 3735 3798 Size: 552 939 24 734 211 246 516 63 Cuts at: 3798 4298 513 Size: 500 3074 Fragments arranged by size: 3074 939 734 552 516 500 246 211 63 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3993 4886 4903 5056 5241 5686 325 Size: 1255 2413 893 17 153 185 445 1498 Fragments arranged by size: 2413 1498 1255 893 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3282 5105 6817 Size: 274 80 33 51 1443 1305 1823 1712 Cuts at: 6817 96 Size: 138 Fragments arranged by size: 1823 1712 1443 1305 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2498 2530 6819 6851 2498 Size: 32 4289 32 2506 Fragments arranged by size: 4289 2506 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6859 XbaI T'CTAG_A Cuts at: 4195 4195 Size: 6859 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6859 XhoI C'TCGA_G Cuts at: 1943 2247 4130 1943 Size: 304 1883 4672 Fragments arranged by size: 4672 1883 304 XmaI C'CCGG_G Cuts at: 1870 3081 4184 1870 Size: 1211 1103 4545 Fragments arranged by size: 4545 1211 1103 XmnI GAAnn'nnTTC Cuts at: 2060 4988 2060 Size: 2928 3931 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4667 276 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI