################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## Acc65I AccI AccII AfaI AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoRV FbaI FseI FspI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsaI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 1240 GACGTC GACGT^C AccIII 1384 TCCGGA T^CCGGA AclI 1363 AACGTT AA^CGTT AcsI 135 GAATTT [GA]^AATT[CT] Aor51HI 1169 AGCGCT AGC^GCT ApaI 1014 GGGCCC GGGCC^C ApoI 135 GAATTT [GA]^AATT[CT] Asp700I 1167 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 295 GAGCTC G[AT]GC[AT]^C BalI 1035 TGGCCA TGG^CCA BamHI 1292 GGATCC G^GATCC BbsI 368 GAAGAC GAAGAC(2/6) BglI 1007 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1107 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1107 GCTAAGC GC^T[ACGT]AGC BpuAI 368 GAAGAC GAAGAC(2/6) BsaI 645 GAGACC GGTCTC(1/5) BsaWI 1384 TCCGGA [AT]^CCGG[AT] BseAI 1384 TCCGGA T^CCGGA BsiHKAI 295 GAGCTC G[AT]GC[AT]^C BsmBI 476 GAGACG CGTCTC(1/5) BspEI 1384 TCCGGA T^CCGGA BspHI 275 TCATGA T^CATGA BspLU11I 1232 ACATGT A^CATGT BspMI 350 ACCTGC ACCTGC(4/8) BssSI 1264 CACGAG CACGAG(-5/-1) BstAPI 549 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 628 GGTGACC G^GT[ACGT]ACC BstPI 628 GGTGACC G^GT[ACGT]ACC BstXI 944 CCACACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1107 GCTAAGC GC^T[ACGT]AGC DraI 1315 TTTAAA TTT^AAA DsaI 67 CCACGG C^C[GA][CT]GG EaeI 1035 TGGCCA [CT]^GGCC[GA] Eco47III 1169 AGCGCT AGC^GCT EcoO65I 628 GGTGACC G^GT[ACGT]ACC EcoT22I 514 ATGCAT ATGCA^T HaeII 1169 AGCGCT [GA]GCGC^[CT] HincII 391 GTCAAC GT[CT]^[GA]AC HindII 391 GTCAAC GT[CT]^[GA]AC HindIII 836 AAGCTT A^AGCTT MluNI 1035 TGGCCA TGG^CCA MroI 1384 TCCGGA T^CCGGA MscI 1035 TGGCCA TGG^CCA NsiI 514 ATGCAT ATGCA^T Psp1406I 1363 AACGTT AA^CGTT PstI 1329 CTGCAG CTGCA^G RcaI 275 TCATGA T^CATGA SacI 295 GAGCTC GAGCT^C SapI 925 GAAGAGC GCTCTTC(1/4) SfiI 1006 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 932 CTCAAG C^T[CT][GA]AG StuI 1421 AGGCCT AGG^CCT TaqI 434 TCGA T^CGA TthHB8I 434 TCGA T^CGA XcmI 650 CCAAGCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1167 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 78 GGCGTC G[GA]^CG[CT]C 1240 GACGTC BanII 295 GAGCTC G[GA]GC[CT]^C 1014 GGGCCC BbvCI 718 CCTCAGC CCTCAGC(-5/-2) 216 GCTGAGG BmyI 295 GAGCTC G[AGT]GC[ACT]^C 1014 GGGCCC BpmI 298 CTCCAG CTGGAG(16/14) 343 CTGGAG BsaHI 78 GGCGTC G[GA]^CG[CT]C 1240 GACGTC BsgI 569 CTGCAC GTGCAG(16/14) 325 GTGCAG Bsp1286I 295 GAGCTC G[AGT]GC[ACT]^C 1014 GGGCCC CfoI 1170 GCGC GCG^C 1374 GCGC EarI 688 GAAGAG CTCTTC(1/4) 925 GAAGAG EcoT14I 659 CCTTGG C^C[AT][AT]GG 1046 CCAAGG HgaI 79 GCGTC GACGC(5/10) 1449 GCGTC HhaI 1170 GCGC GCG^C 1374 GCGC Hin1I 78 GGCGTC G[GA]^CG[CT]C 1240 GACGTC HinP1I 1170 GCGC G^CGC 1374 GCGC Ksp632I 688 GAAGAG CTCTTC(1/4) 925 GAAGAG MseI 1091 TTAA T^TAA 1316 TTAA PflMI 374 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 485 CCAGCCAGTGG PpuMI 36 AGGACCC [GA]G^G[AT]CC[CT] 347 AGGACCT PvuII 150 CAGCTG CAG^CTG 1432 CAGCTG SfcI 386 CTATAG C^T[GA][CT]AG 1329 CTGCAG StyI 659 CCTTGG C^C[AT][AT]GG 1046 CCAAGG TfiI 873 GAATC G^A[AT]TC 964 GAATC Tru9I 1091 TTAA T^TAA 1316 TTAA Tsp509I 10 AATT ^AATT 136 AATT Van91I 374 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 485 CCAGCCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AflIII AluI AlwI AlwNI AvaII BbvI BcnI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XhoII