Restriction map for
pFN21AE3219
(Circular) (Six-base) MAPSORT of: pFN21AE3219.seq Check: 7726 from: 1 to: 6190
pFN21AE3219 6190 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3249 4000 278
Size: 53 83 186 936 1713 751 2468
Fragments arranged by size:
2468 1713 936 751 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 3369 4317 4690 1563
Size: 1806 948 373 3063
Fragments arranged by size:
3063 1806 948 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2469 2699 4253 5637 5905 1632
Size: 837 230 1554 1384 268 1917
Fragments arranged by size:
1917 1554 1384 837 268 230
AfeI AGC'GCT
Cuts at: 3176 3176
Size: 6190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 2484 3078 3237 5089 6066 1203
Size: 1281 594 159 1852 977 1327
Fragments arranged by size:
1852 1327 1281 977 594 159
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 2954 1166
Size: 1788 4402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AlwNI CAG_nnn'CTG
Cuts at: 2188 2254 2560 3014 3933 5505 2188
Size: 66 306 454 919 1572 2873
Fragments arranged by size:
2873 1572 919 454 306 66
ApaI G_GGCC'C
Cuts at: 3023 3023
Size: 6190
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 2140 3499 3725 3877 5986 1493
Size: 647 1359 226 152 2109 1697
Fragments arranged by size:
2109 1697 1359 647 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 3515 1870
Size: 73 1572 4545
Fragments arranged by size:
4545 1572 73
BamHI G'GATC_C
Cuts at: 3297 3520 3887 3297
Size: 223 367 5600
Fragments arranged by size:
5600 367 223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3511 4966 618
Size: 325 217 627 1724 1455 1842
Fragments arranged by size:
1842 1724 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2304 3023 3509 1391
Size: 913 719 486 4072
Fragments arranged by size:
4072 913 719 486
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2380 928
Size: 971 481 4738
Fragments arranged by size:
4738 971 481
BbvCI CC'TCA_GC
Cuts at: 2222 2724 2222
Size: 502 5688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4381 4415 1903
Size: 34 2444 34 3678
Fragments arranged by size:
3678 2444 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4082 5292 4082
Size: 1210 4980
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6190
BfrBI ATG'CAT
Cuts at: 2521 2521
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3018 4801 136
Size: 107 122 71 631 605 1346 1783 1525
Fragments arranged by size:
1783 1525 1346 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6185 6185
Size: 6190
BlpI GC'TnA_GC
Cuts at: 3113 3607 3113
Size: 494 5696
Bme1580I G_kGCm'C
Cuts at: 1790 3023 4251 5407 1790
Size: 1233 1228 1156 2573
Fragments arranged by size:
2573 1233 1228 1156
BmgBI CAC'GTC
Cuts at: 1318 2485 3079 1318
Size: 1167 594 4429
Fragments arranged by size:
4429 1167 594
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3921 4879 442
Size: 416 53 83 399 24 2504 958 1753
Fragments arranged by size:
2504 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2369 4850 1239
Size: 1049 81 2481 2579
Fragments arranged by size:
2579 2481 1049 81
Bpu10I CC'TnA_GC
Cuts at: 2222 2724 2222
Size: 502 5688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2922 4315 5180 5478 2922
Size: 1393 865 298 3634
Fragments arranged by size:
3634 1393 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2644 4853 882
Size: 617 1145 2209 2219
Fragments arranged by size:
2219 2209 1145 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 6184 6184
Size: 6190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 3246 3997 4379
Size: 53 83 186 936 551 1162 751 382
Cuts at: 4379 5014 275
Size: 635 1451
Fragments arranged by size:
1451 1162 936 751 635 551 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3389 4623 5021 5295 5442 5853 3389
Size: 1234 398 274 147 411 3726
Fragments arranged by size:
3726 1234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3302 3332 1178
Size: 30 165 30 1899 30 4036
Fragments arranged by size:
4036 1899 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2494 2880 2905 1186
Size: 994 314 386 25 4471
Fragments arranged by size:
4471 994 386 314 25
BseYI C'CCAG_C
Cuts at: 3108 3439 5393 5889 3108
Size: 331 1954 496 3409
Fragments arranged by size:
3409 1954 496 331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 2559 1163
Size: 573 615 208 4794
Fragments arranged by size:
4794 615 573 208
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 4251 4336 5407 2304
Size: 1947 85 1071 3087
Fragments arranged by size:
3087 1947 1071 85
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
BsmBI CGTCTCn'nnnn_
Cuts at: 2475 2475
Size: 6190
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 3023 3509 4251 4336 5407 1391
Size: 399 514 719 486 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 514 486 399 85
BspEI T'CCGG_A
Cuts at: 3389 5853 3389
Size: 2464 3726
BspHI T'CATG_A
Cuts at: 2280 4079 5809 2280
Size: 1799 1730 2661
Fragments arranged by size:
2661 1799 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 3545 844
Size: 1520 1181 3489
Fragments arranged by size:
3489 1520 1181
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4685 4859 65
Size: 1183 3437 174 1396
Fragments arranged by size:
3437 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4834 5021 1700
Size: 110 3024 187 2869
Fragments arranged by size:
3024 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 3269 4250 5262 3269
Size: 981 1012 4197
Fragments arranged by size:
4197 1012 981
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2560 794
Size: 1766 4424
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 2633 1184
Size: 1449 4741
BstXI CCAn_nnnn'nTGG
Cuts at: 2956 2956
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2250 2556 2674 3297 3520 3887 4272 4289
Size: 278 306 118 623 223 367 385 17
Cuts at: 4289 5741 5827 5839 6185 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 623 385 367 346 306 278
223 118 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2072 513
Size: 552 939 68 4631
Fragments arranged by size:
4631 939 552 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 3706 4500 4520 3706
Size: 794 20 5376
Fragments arranged by size:
5376 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 3322 3494 3322
Size: 172 6018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3040 3637 4526
Size: 54 1425 50 275 18 1210 597 889
Cuts at: 4526 6155 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1210 889 597 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 2925 4120 1353
Size: 1335 237 1195 3423
Fragments arranged by size:
3423 1335 1195 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4777 5151 5297 387
Size: 1064 138 3188 374 146 1280
Fragments arranged by size:
3188 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2302 2302
Size: 6190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2369 2469 2699 4253 4850 5637
Size: 393 656 81 100 230 1554 597 787
Cuts at: 5637 5905 1239
Size: 268 1524
Fragments arranged by size:
1554 1524 787 656 597 393 268 230
100 81
EcoO109I rG'GnC_Cy
Cuts at: 2042 2353 3011 3055 3231 2042
Size: 311 658 44 176 5001
Fragments arranged by size:
5001 658 311 176 44
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2435 2680 3196 2435
Size: 245 516 5429
Fragments arranged by size:
5429 516 245
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 3178 5337 1678
Size: 1500 2159 2531
Fragments arranged by size:
2531 2159 1500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 2728 2760 2900
Size: 32 135 32 1055 32 69 32 140
Cuts at: 2900 2932 4831 4863 4905 4937 1373
Size: 32 1899 32 42 32 2626
Fragments arranged by size:
2626 1899 1055 140 135 69 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2398 3534 3780 669
Size: 988 281 460 1136 246 3079
Fragments arranged by size:
3079 1136 988 460 281 246
HindIII A'AGCT_T
Cuts at: 748 2841 748
Size: 2093 4097
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2151 3149 5304 5488 1305
Size: 846 998 2155 184 2007
Fragments arranged by size:
2155 2007 998 846 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3042 10
Size: 54 1425 343 1210 3158
Fragments arranged by size:
3158 1425 1210 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2026 2264 2954 3036 4148 4507 4666
Size: 648 860 238 690 82 1112 359 159
Cuts at: 4666 518
Size: 2042
Fragments arranged by size:
2042 1112 860 690 648 359 238 159
82
MspA1I CmG'CkG
Cuts at: 1606 2157 2856 3439 3606 4283 5431 5676 1606
Size: 551 699 583 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 699 677 583 551 245 167
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4699
Fragments arranged by size:
4699 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NsiI A_TGCA'T
Cuts at: 2523 2523
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 2263 3075 3241 3548 5093 1132
Size: 97 1034 812 166 307 1545 2229
Fragments arranged by size:
2229 1545 1034 812 307 166 97
PasI CC'CwG_GG
Cuts at: 1348 2057 2565 1348
Size: 709 508 4973
Fragments arranged by size:
4973 709 508
PciI A'CATG_T
Cuts at: 3237 5089 3237
Size: 1852 4338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2385 2496 1108
Size: 1277 111 4802
Fragments arranged by size:
4802 1277 111
PfoI T'CCnGG_A
Cuts at: 2253 2582 2253
Size: 329 5861
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2042 2353 2042
Size: 311 5879
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PspOMI G'GGCC_C
Cuts at: 3019 3019
Size: 6190
PstI C_TGCA'G
Cuts at: 830 3338 3542 830
Size: 2508 204 3478
Fragments arranged by size:
3478 2508 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 2157 3439 2157
Size: 1282 4908
SacI G_AGCT'C
Cuts at: 2304 2304
Size: 6190
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 2925 2925
Size: 6190
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2391 3334 3538 4673 5354 5545 826
Size: 219 1346 943 204 1135 681 191 1471
Fragments arranged by size:
1471 1346 1135 943 681 219 204 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3018 3018
Size: 6190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3517 1872
Size: 1645 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2937 4294 5195 5457 820
Size: 197 926 994 1357 901 262 1553
Fragments arranged by size:
1553 1357 994 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3548 1132
Size: 2416 3774
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 3428 1517
Size: 1911 4279
StyI C'CwwG_G
Cuts at: 513 1065 2004 2664 3051 3629 513
Size: 552 939 660 387 578 3074
Fragments arranged by size:
3074 939 660 578 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3048 3398 4217 4234 4387 4572 5017
Size: 1255 1468 350 819 17 153 185 445
Cuts at: 5017 325
Size: 1498
Fragments arranged by size:
1498 1468 1255 819 445 350 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4436 6148 96
Size: 274 80 33 51 1443 2459 1712 138
Fragments arranged by size:
2459 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6150 6182 6150
Size: 32 6158
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2662 2662
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 3515 1870
Size: 1645 4545
XmnI GAAnn'nnTTC
Cuts at: 3176 4319 3176
Size: 1143 5047
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3247 3998 276
Size: 53 83 186 936 1713 751 2468
Fragments arranged by size:
2468 1713 936 751 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI
RsrII SacII SanDI SexAI SfoI SrfI SwaI