(Linear) (Six-base) MAPSORT of: KIEE3253.seq Check: 6618 from: 1 to: 1767 KIEE3253.orf With 182 enzymes: SgfI * July 3, 2012 17:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 556 1767 Size: 556 1211 AatII G_ACGT'C Cuts at: 0 621 1767 Size: 621 1146 Acc65I G'GTAC_C Cuts at: 0 480 1767 Size: 480 1287 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 623 1767 Size: 238 385 1144 Fragments arranged by size: 1144 385 238 AfeI AGC'GCT Cuts at: 0 427 1767 Size: 427 1340 AleI CACnn'nnGTG Cuts at: 0 304 1444 1767 Size: 304 1140 323 Fragments arranged by size: 1140 323 304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 416 1462 1767 Size: 416 1046 305 Fragments arranged by size: 1046 416 305 AlwNI CAG_nnn'CTG Cuts at: 0 559 687 1530 1767 Size: 559 128 843 237 Fragments arranged by size: 843 559 237 128 ApaLI G'TGCA_C Cuts at: 0 401 1767 Size: 401 1366 ApoI r'AATT_y Cuts at: 0 22 847 1767 Size: 22 825 920 Fragments arranged by size: 920 825 22 AvaI C'yCGr_G Cuts at: 0 698 1767 Size: 698 1069 BanI G'GyrC_C Cuts at: 0 80 480 1438 1599 1767 Size: 80 400 958 161 168 Fragments arranged by size: 958 400 168 161 80 BanII G_rGCy'C Cuts at: 0 228 627 1767 Size: 228 399 1140 Fragments arranged by size: 1140 399 228 BbsI GAAGACnn'nnnn_ Cuts at: 0 264 1490 1767 Size: 264 1226 277 Fragments arranged by size: 1226 277 264 BbvCI CC'TCA_GC Cuts at: 0 631 1258 1767 Size: 631 627 509 Fragments arranged by size: 631 627 509 BglI GCCn_nnn'nGGC Cuts at: 0 320 764 1133 1436 1767 Size: 320 444 369 303 331 Fragments arranged by size: 444 369 331 320 303 Bme1580I G_kGCm'C Cuts at: 0 311 405 1093 1767 Size: 311 94 688 674 Fragments arranged by size: 688 674 311 94 BmrI ACTGGGnnnn_n' Cuts at: 0 14 1767 Size: 14 1753 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 63 1767 Size: 63 1704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 955 1507 1678 1767 Size: 955 552 171 89 Fragments arranged by size: 955 552 171 89 Bpu10I CC'TnA_GC Cuts at: 0 631 1258 1767 Size: 631 627 509 Fragments arranged by size: 631 627 509 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1203 1767 Size: 1203 564 BsaI GGTCTCn'nnnn_ Cuts at: 0 60 736 1767 Size: 60 676 1031 Fragments arranged by size: 1031 676 60 BsaHI Gr'CG_yC Cuts at: 0 530 618 1767 Size: 530 88 1149 Fragments arranged by size: 1149 530 88 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 39 48 51 54 57 151 451 556 Size: 39 9 3 3 3 94 300 105 Cuts at: 556 679 988 1228 1231 1240 1767 Size: 123 309 240 3 9 527 Fragments arranged by size: 527 309 300 240 123 105 94 39 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 310 475 606 961 1026 1453 1737 1767 Size: 310 165 131 355 65 427 284 30 Fragments arranged by size: 427 355 310 284 165 131 65 30 BsiEI CG_ry'CG Cuts at: 0 660 1767 Size: 660 1107 BsiHKAI G_wGCw'C Cuts at: 0 405 627 1767 Size: 405 222 1140 Fragments arranged by size: 1140 405 222 BsmI GAATG_Cn' Cuts at: 0 793 1374 1767 Size: 793 581 393 Fragments arranged by size: 793 581 393 Bsp1286I G_dGCh'C Cuts at: 0 228 311 405 627 1093 1767 Size: 228 83 94 222 466 674 Fragments arranged by size: 674 466 228 222 94 83 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 556 754 1767 Size: 556 198 1013 Fragments arranged by size: 1013 556 198 BsrFI r'CCGG_y Cuts at: 0 572 1761 1767 Size: 572 1189 6 Fragments arranged by size: 1189 572 6 BssSI C'ACGA_G Cuts at: 0 409 1767 Size: 409 1358 BstAPI GCAn_nnn'nTGC Cuts at: 0 687 773 1767 Size: 687 86 994 Fragments arranged by size: 994 687 86 BstYI r'GATC_y Cuts at: 0 728 1485 1701 1767 Size: 728 757 216 66 Fragments arranged by size: 757 728 216 66 BtgI C'CryG_G Cuts at: 0 315 833 1767 Size: 315 518 934 Fragments arranged by size: 934 518 315 BtsI GCAGTG_nn' Cuts at: 0 463 685 717 1767 Size: 463 222 32 1050 Fragments arranged by size: 1050 463 222 32 ClaI AT'CG_AT Cuts at: 0 656 1767 Size: 656 1111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 337 372 1767 Size: 337 35 1395 Fragments arranged by size: 1395 337 35 DrdI GACnn_nn'nnGTC Cuts at: 0 553 1767 Size: 553 1214 EaeI y'GGCC_r Cuts at: 0 570 1001 1310 1467 1767 Size: 570 431 309 157 300 Fragments arranged by size: 570 431 309 300 157 EarI CTCTTCn'nnn_ Cuts at: 0 10 75 114 1217 1767 Size: 10 65 39 1103 550 Fragments arranged by size: 1103 550 65 39 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 409 1767 Size: 409 1358 EcoICRI GAG'CTC Cuts at: 0 625 1767 Size: 625 1142 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 623 955 1507 1678 1767 Size: 238 385 332 552 171 89 Fragments arranged by size: 552 385 332 238 171 89 EcoO109I rG'GnC_Cy Cuts at: 0 145 939 978 1549 1767 Size: 145 794 39 571 218 Fragments arranged by size: 794 571 218 145 39 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1447 1767 Size: 1447 320 FspI TGC'GCA Cuts at: 0 386 1767 Size: 386 1381 HaeII r_GCGC'y Cuts at: 0 240 429 1767 Size: 240 189 1338 Fragments arranged by size: 1338 240 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 34 66 517 549 1767 Size: 34 32 451 32 1218 Fragments arranged by size: 1218 451 34 32 32 HindIII A'AGCT_T Cuts at: 0 463 1767 Size: 463 1304 KpnI G_GTAC'C Cuts at: 0 484 1767 Size: 484 1283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 425 557 884 1767 Size: 425 132 327 883 Fragments arranged by size: 883 425 327 132 MscI TGG'CCA Cuts at: 0 1003 1312 1767 Size: 1003 309 455 Fragments arranged by size: 1003 455 309 MslI CAynn'nnrTG Cuts at: 0 304 1444 1767 Size: 304 1140 323 Fragments arranged by size: 1140 323 304 MspA1I CmG'CkG Cuts at: 0 33 287 475 752 1040 1262 1276 1316 Size: 33 254 188 277 288 222 14 40 Cuts at: 1316 1388 1767 Size: 72 379 Fragments arranged by size: 379 288 277 254 222 188 72 40 33 14 NcoI C'CATG_G Cuts at: 0 833 1767 Size: 833 934 PasI CC'CwG_GG Cuts at: 0 149 948 1767 Size: 149 799 819 Fragments arranged by size: 819 799 149 PfoI T'CCnGG_A Cuts at: 0 1358 1655 1767 Size: 1358 297 112 Fragments arranged by size: 1358 297 112 PstI C_TGCA'G Cuts at: 0 458 683 751 757 814 902 1045 1767 Size: 458 225 68 6 57 88 143 722 Fragments arranged by size: 722 458 225 143 88 68 57 6 PvuI CG_AT'CG Cuts at: 0 660 1767 Size: 660 1107 PvuII CAG'CTG Cuts at: 0 33 475 752 1040 1262 1276 1316 1388 Size: 33 442 277 288 222 14 40 72 Cuts at: 1388 1767 Size: 379 Fragments arranged by size: 442 379 288 277 222 72 40 33 14 SacI G_AGCT'C Cuts at: 0 627 1767 Size: 627 1140 SapI GCTCTTCn'nnn_ Cuts at: 0 114 1767 Size: 114 1653 SfcI C'TryA_G Cuts at: 0 454 595 679 747 753 810 898 1041 Size: 454 141 84 68 6 57 88 143 Cuts at: 1041 1338 1767 Size: 297 429 Fragments arranged by size: 454 429 297 143 141 88 84 68 57 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1133 1767 Size: 1133 634 SmaI CCC'GGG Cuts at: 0 700 1767 Size: 700 1067 SmlI C'TyrA_G Cuts at: 0 1182 1767 Size: 1182 585 SpeI A'CTAG_T Cuts at: 0 1093 1291 1767 Size: 1093 198 476 Fragments arranged by size: 1093 476 198 StuI AGG'CCT Cuts at: 0 1127 1767 Size: 1127 640 StyI C'CwwG_G Cuts at: 0 833 1767 Size: 833 934 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 42 68 975 1132 1136 1467 1767 Size: 42 26 907 157 4 331 300 Fragments arranged by size: 907 331 300 157 42 26 4 TatI w'GTAC_w Cuts at: 0 325 1767 Size: 325 1442 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1592 1624 1767 Size: 1592 32 143 Fragments arranged by size: 1592 143 32 Tth111I GACn'n_nGTC Cuts at: 0 1732 1767 Size: 1732 35 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 840 1330 1767 Size: 840 490 437 Fragments arranged by size: 840 490 437 XmaI C'CCGG_G Cuts at: 0 698 1767 Size: 698 1069 ZraI GAC'GTC Cuts at: 0 619 1767 Size: 619 1148 Enzymes that do cut: AarI AatII Acc65I AcuI AfeI AleI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssSI BstAPI BstYI BtgI BtsI ClaI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NcoI PasI PfoI PstI PvuI PvuII SacI SapI SfcI SfiI SmaI SmlI SpeI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AccI AclI AflII AflIII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI DraI DraIII EagI EcoNI EcoRI EcoRV FseI FspAI HincII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI RsrII SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SphI SrfI SspI SwaI XbaI XhoI XmnI