################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbrPI BbsI BciVI BfrI BglII BlnI BpuAI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI HincII HindII HindIII HpaI KpnI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 5 GGCGCC G[GA]^CG[CT]C AfaI 1881 GTAC GT^AC AflIII 1493 ACATGT A^C[GA][CT]GT AviII 1701 TGCGCA TGC^GCA BamHI 34 GGATCC G^GATCC BbeI 5 GGCGCC GGCGC^C BclI 1428 TGATCA T^GATCA BsaAI 2497 CACGTA [CT]AC^GT[GA] BsaBI 2805 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 5 GGCGCC G[GA]^CG[CT]C BsaWI 1944 ACCGGA [AT]^CCGG[AT] BsmBI 2587 GAGACG CGTCTC(1/5) BsmI 2910 GAATGC GAATGC(1/-1) BspLU11I 1493 ACATGT A^CATGT EarI 727 CTCTTC CTCTTC(1/4) EcoRV 1150 GATATC GAT^ATC FbaI 1428 TGATCA T^GATCA FspI 1701 TGCGCA TGC^GCA Hin1I 5 GGCGCC G[GA]^CG[CT]C KasI 5 GGCGCC G^GCGCC Ksp632I 727 CTCTTC CTCTTC(1/4) KspI 10 CCGCGG CCGC^GG MamI 2805 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 5 GGCGCC GG^CGCC NcoI 1860 CCATGG C^CATGG PshAI 1320 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 1881 GTAC GT^AC SacII 10 CCGCGG CCGC^GG SfoI 5 GGCGCC GGC^GCC SmaI 84 CCCGGG CCC^GGG SmlI 2920 CTCAAG C^T[CT][GA]AG SphI 1806 GCATGC GCATG^C TaqI 2765 TCGA T^CGA TfiI 2082 GATTC G^A[AT]TC TthHB8I 2765 TCGA T^CGA XmaCI 84 CCCGGG C^CCGGG XmaI 84 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Alw44I 1489 GTGCAC G^TGCAC 2719 GTGCAC AocI 1499 CCTCAGG CC^T[ACGT]AGG 1849 CCTGAGG ApaI 1856 GGGCCC GGGCC^C 2352 GGGCCC ApaLI 1489 GTGCAC G^TGCAC 2719 GTGCAC AspI 398 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2767 GACATGGTC AvaI 28 CTCGGG C^[CT]CG[GA]G 84 CCCGGG BbvCI 1385 CCTCAGC CCTCAGC(-5/-2) 2616 CCTCAGC BcgI 2316 GCATGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2408 GCACCGTCATCG BfaI 949 CTAG C^TAG 1333 CTAG BglI 83 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1346 GCCCACTGGGC BsoBI 28 CTCGGG C^[CT]CG[GA]G 84 CCCGGG BstXI 633 CCACCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2841 CCAGCGGCCTGG BstYI 34 GGATCC [GA]^GATC[CT] 1472 AGATCC Bsu36I 1499 CCTCAGG CC^T[ACGT]AGG 1849 CCTGAGG Eco81I 1499 CCTCAGG CC^T[ACGT]AGG 1849 CCTGAGG EcoNI 702 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2124 CCTTGCCAAGG MaeI 949 CTAG C^TAG 1333 CTAG MaeII 2498 ACGT A^CGT 2573 ACGT MflI 34 GGATCC [GA]^GATC[CT] 1472 AGATCC NaeI 2043 GCCGGC GCC^GGC 2625 GCCGGC NgoMIV 2043 GCCGGC G^CCGGC 2625 GCCGGC PflFI 398 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2767 GACATGGTC PflMI 1899 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2129 CCAAGGAGTGG PleI 823 GACTC GAGTC(4/5) 919 GACTC PpuMI 785 GGGACCT [GA]G^G[AT]CC[CT] 1886 AGGACCT SexAI 399 ACCTGGT A^CC[AT]GGT 2513 ACCTGGT Tth111I 398 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2767 GACATGGTC Van91I 1899 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2129 CCAAGGAGTGG XhoII 34 GGATCC [GA]^GATC[CT] 1472 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI