(Circular) (Six-base) MAPSORT of: pFN21AB8541.seq Check: 5018 from: 1 to: 7642 pFN21AB8541 7642 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2574 2818 3262 3749 4119 2574 Size: 244 444 487 370 6097 Fragments arranged by size: 6097 487 444 370 244 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5452 278 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4985 1937 Size: 3048 4594 Acc65I G'GTAC_C Cuts at: 4963 4963 Size: 7642 AclI AA'CG_TT Cuts at: 1563 5769 6142 1563 Size: 4206 373 3063 Fragments arranged by size: 4206 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2719 2739 4283 4592 5705 7089 7357 1632 Size: 1087 20 1544 309 1113 1384 268 1917 Fragments arranged by size: 1917 1544 1384 1113 1087 309 268 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7445 AflIII A'CryG_T Cuts at: 1203 3498 6541 7518 1203 Size: 2295 3043 977 1327 Fragments arranged by size: 3043 2295 1327 977 AgeI A'CCGG_T Cuts at: 6473 6473 Size: 7642 AhdI GACnn_n'nnGTC Cuts at: 6371 6371 Size: 7642 AleI CACnn'nnGTG Cuts at: 1166 3746 4731 1166 Size: 2580 985 4077 Fragments arranged by size: 4077 2580 985 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3015 1184 Size: 1831 5811 AlwNI CAG_nnn'CTG Cuts at: 2142 3016 3103 3577 3895 5385 6957 2142 Size: 874 87 474 318 1490 1572 2827 Fragments arranged by size: 2827 1572 1490 874 474 318 87 ApaI G_GGCC'C Cuts at: 3865 4361 3865 Size: 496 7146 ApaLI G'TGCA_C Cuts at: 3494 4724 5699 6855 3494 Size: 1230 975 1156 4281 Fragments arranged by size: 4281 1230 1156 975 ApoI r'AATT_y Cuts at: 1493 4951 5177 5329 7438 1493 Size: 3458 226 152 2109 1697 Fragments arranged by size: 3458 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6196 160 Size: 872 5164 1606 Fragments arranged by size: 5164 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2089 4967 1870 Size: 73 90 56 2878 4545 Fragments arranged by size: 4545 2878 90 73 56 BamHI G'GATC_C Cuts at: 2039 4972 5339 2039 Size: 2933 367 4342 Fragments arranged by size: 4342 2933 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2010 2392 2881 3274 4487 Size: 325 217 627 223 382 489 393 1213 Cuts at: 4487 4754 4963 6418 618 Size: 267 209 1455 1842 Fragments arranged by size: 1842 1455 1213 627 489 393 382 325 267 223 217 209 BanII G_rGCy'C Cuts at: 1391 2367 2681 3645 3865 4361 4961 1391 Size: 976 314 964 220 496 600 4072 Fragments arranged by size: 4072 976 964 600 496 314 220 BbeI G_GCGC'C Cuts at: 2014 2014 Size: 7642 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6671 BbvCI CC'TCA_GC Cuts at: 3391 4622 3391 Size: 1231 6411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4310 4344 4402 4436 5833 Size: 34 50 34 2289 34 58 34 1397 Cuts at: 5833 5867 1903 Size: 34 3678 Fragments arranged by size: 3678 2289 1397 58 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5534 6744 5534 Size: 1210 6432 BclI T'GATC_A Cuts at: 1548 3433 1548 Size: 1885 5757 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2094 3357 6253 Size: 107 122 71 631 605 422 1263 2896 Cuts at: 6253 136 Size: 1525 Fragments arranged by size: 2896 1525 1263 631 605 422 122 107 71 BglII A'GATC_T Cuts at: 7637 7637 Size: 7642 BlpI GC'TnA_GC Cuts at: 2078 2538 3486 5059 2078 Size: 460 948 1573 4661 Fragments arranged by size: 4661 1573 948 460 Bme1580I G_kGCm'C Cuts at: 1790 2116 3498 3865 4361 4643 4728 5703 6859 Size: 326 1382 367 496 282 85 975 1156 Cuts at: 6859 1790 Size: 2573 Fragments arranged by size: 2573 1382 1156 975 496 367 326 282 85 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2231 2305 3365 Size: 416 53 83 399 24 814 74 1060 Cuts at: 3365 3976 5373 6331 442 Size: 611 1397 958 1753 Fragments arranged by size: 1753 1397 1060 958 814 611 416 399 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7642 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3091 4507 3091 Size: 1416 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2436 2647 3241 3494 4850 6302 1239 Size: 1197 211 594 253 1356 1452 2579 Fragments arranged by size: 2579 1452 1356 1197 594 253 211 Bpu10I CC'TnA_GC Cuts at: 3066 3391 3446 4622 3066 Size: 325 55 1176 6086 Fragments arranged by size: 6086 1176 325 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4910 5767 6632 6930 4910 Size: 857 865 298 5622 Fragments arranged by size: 5622 865 857 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2036 2054 2395 2674 6305 882 Size: 617 537 18 341 279 3631 2219 Fragments arranged by size: 3631 2219 617 537 341 279 18 BsaAI yAC'GTr Cuts at: 493 1204 4504 5433 493 Size: 711 3300 929 2702 Fragments arranged by size: 3300 2702 929 711 BsaBI GATnn'nnATC Cuts at: 4814 7636 4814 Size: 2822 4820 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2011 5449 5831 6466 Size: 53 83 186 936 478 3438 382 635 Cuts at: 6466 275 Size: 1451 Fragments arranged by size: 3438 1451 936 635 478 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3949 6075 6473 6747 6894 7305 3949 Size: 2126 398 274 147 411 4286 Fragments arranged by size: 4286 2126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3123 3153 1178 Size: 30 165 30 1720 30 5667 Fragments arranged by size: 5667 1720 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2506 3280 3692 4058 1186 Size: 865 455 774 412 366 4770 Fragments arranged by size: 4770 865 774 455 412 366 BseYI C'CCAG_C Cuts at: 2463 2504 2814 2867 2983 3400 3767 4010 6845 Size: 41 310 53 116 417 367 243 2835 Cuts at: 6845 7341 2463 Size: 496 2764 Fragments arranged by size: 2835 2764 496 417 367 310 243 116 53 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3011 3135 4396 4601 1163 Size: 573 1275 124 1261 205 4204 Fragments arranged by size: 4204 1275 1261 573 205 124 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5092 5853 6881 1540 Size: 275 186 3091 761 1028 2301 Fragments arranged by size: 3091 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2265 2565 3498 4533 4728 5703 5788 6859 2265 Size: 300 933 1035 195 975 85 1071 3048 Fragments arranged by size: 3048 1071 1035 975 933 300 195 85 BsmI GAATG_Cn' Cuts at: 4921 5153 5246 4921 Size: 232 93 7317 Fragments arranged by size: 7317 232 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4586 4586 Size: 7642 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2265 2367 2565 2681 3498 3645 Size: 399 326 149 102 198 116 817 147 Cuts at: 3645 3865 4361 4533 4643 4728 4961 5703 5788 Size: 220 496 172 110 85 233 742 85 Cuts at: 5788 6859 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 817 742 496 399 326 233 220 198 172 149 147 116 110 102 85 85 BspEI T'CCGG_A Cuts at: 7305 7305 Size: 7642 BspHI T'CATG_A Cuts at: 5531 7261 5531 Size: 1730 5912 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2574 2620 2818 3262 3749 4119 4997 844 Size: 1730 46 198 444 487 370 878 3489 Fragments arranged by size: 3489 1730 878 487 444 370 198 46 BsrBI CCG'CTC Cuts at: 1394 1823 4407 4707 4866 5529 1394 Size: 429 2584 300 159 663 3507 Fragments arranged by size: 3507 2584 663 429 300 159 BsrDI GCAATG_nn' Cuts at: 65 1248 6137 6311 65 Size: 1183 4889 174 1396 Fragments arranged by size: 4889 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3178 4048 4218 4630 4645 4751 6286 Size: 110 1368 870 170 412 15 106 1535 Cuts at: 6286 6473 1700 Size: 187 2869 Fragments arranged by size: 2869 1535 1368 870 412 187 170 110 106 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7642 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7642 BssSI C'ACGA_G Cuts at: 5702 6714 5702 Size: 1012 6630 BstAPI GCAn_nnn'nTGC Cuts at: 794 2142 2569 3103 794 Size: 1348 427 534 5333 Fragments arranged by size: 5333 1348 534 427 BstBI TT'CG_AA Cuts at: 6446 6446 Size: 7642 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7642 BstXI CCAn_nnnn'nTGG Cuts at: 2645 4853 2645 Size: 2208 5434 BstYI r'GATC_y Cuts at: 1972 2039 3477 4972 5339 5724 5741 7193 7279 Size: 67 1438 1495 367 385 17 1452 86 Cuts at: 7279 7291 7637 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1495 1452 1438 385 367 346 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 3505 3855 3505 Size: 350 7292 BtgI C'CryG_G Cuts at: 513 1065 2015 2116 3372 3865 4074 4899 513 Size: 552 950 101 1256 493 209 825 3256 Fragments arranged by size: 3256 1256 950 825 552 493 209 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4360 4801 487 Size: 683 56 3134 441 3328 Fragments arranged by size: 3328 3134 683 441 56 BtsI GCAGTG_nn' Cuts at: 2192 3240 3976 4466 4726 4738 5158 5952 5972 Size: 1048 736 490 260 12 420 794 20 Cuts at: 5972 2192 Size: 3862 Fragments arranged by size: 3862 1048 794 736 490 420 260 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7642 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7607 DraI TTT'AAA Cuts at: 4946 4946 Size: 7642 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6649 809 Size: 507 5333 1802 Fragments arranged by size: 5333 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2776 3018 3912 Size: 54 1425 50 275 18 946 242 894 Cuts at: 3912 4475 4628 5089 5978 7607 8 Size: 563 153 461 889 1629 43 Fragments arranged by size: 1629 1425 946 894 889 563 461 275 242 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5089 1537 Size: 275 3277 4090 Fragments arranged by size: 4090 3277 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2738 5572 1353 Size: 1385 2834 3423 Fragments arranged by size: 3423 2834 1385 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2440 4919 6229 6603 6749 387 Size: 1064 138 851 2479 1310 374 146 1280 Fragments arranged by size: 2479 1310 1280 1064 851 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2436 2647 2719 2739 3241 3494 4283 Size: 393 804 211 72 20 502 253 789 Cuts at: 4283 4592 4850 5705 6302 7089 7357 1239 Size: 309 258 855 597 787 268 1524 Fragments arranged by size: 1524 855 804 789 787 597 502 393 309 268 258 253 211 72 20 EcoNI CCTnn'n_nnAGG Cuts at: 2711 4133 4937 2711 Size: 1422 804 5416 Fragments arranged by size: 5416 1422 804 EcoO109I rG'GnC_Cy Cuts at: 2791 2801 3851 3861 3892 4147 4357 4930 2791 Size: 10 1050 10 31 255 210 573 5503 Fragments arranged by size: 5503 1050 573 255 210 31 10 10 EcoRI G'AATT_C Cuts at: 4951 5329 4951 Size: 378 7264 EcoRV GAT'ATC Cuts at: 3157 3157 Size: 7642 FspI TGC'GCA Cuts at: 3708 6148 3708 Size: 2440 5202 HaeII r_GCGC'y Cuts at: 1678 2014 2106 3838 6789 1678 Size: 336 92 1732 2951 2531 Fragments arranged by size: 2951 2531 1732 336 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2392 2424 3141 3173 3528 Size: 32 135 32 820 32 717 32 355 Cuts at: 3528 3560 6283 6315 6357 6389 1373 Size: 32 2723 32 42 32 2626 Fragments arranged by size: 2723 2626 820 717 355 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4986 5232 669 Size: 988 281 3048 246 3079 Fragments arranged by size: 3079 3048 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7642 HpaI GTT'AAC Cuts at: 5232 5232 Size: 7642 KasI G'GCGC_C Cuts at: 2010 2010 Size: 7642 KpnI G_GTAC'C Cuts at: 4967 4967 Size: 7642 MfeI C'AATT_G Cuts at: 5241 5241 Size: 7642 MluI A'CGCG_T Cuts at: 7518 7518 Size: 7642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2269 2290 3127 4506 6756 6940 1305 Size: 964 21 837 1379 2250 184 2007 Fragments arranged by size: 2250 2007 1379 964 837 184 21 MscI TGG'CCA Cuts at: 10 64 1489 1832 2778 3914 4477 10 Size: 54 1425 343 946 1136 563 3175 Fragments arranged by size: 3175 1425 1136 946 563 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3527 3746 4424 4506 4721 4731 4772 Size: 648 2361 219 678 82 215 10 41 Cuts at: 4772 4839 5600 5959 6118 518 Size: 67 761 359 159 2042 Fragments arranged by size: 2361 2042 761 678 648 359 219 215 159 82 67 41 10 MspA1I CmG'CkG Cuts at: 1606 2017 2987 3023 3058 3686 4363 4438 4626 Size: 411 970 36 35 628 677 75 188 Cuts at: 4626 4849 5058 5735 6883 7128 1606 Size: 223 209 677 1148 245 2120 Fragments arranged by size: 2120 1148 970 677 677 628 411 245 223 209 188 75 36 35 NaeI GCC'GGC Cuts at: 4050 4632 4050 Size: 582 7060 NarI GG'CG_CC Cuts at: 2011 2011 Size: 7642 NcoI C'CATG_G Cuts at: 513 1065 3865 513 Size: 552 2800 4290 Fragments arranged by size: 4290 2800 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7642 NgoMIV G'CCGG_C Cuts at: 4048 4630 4048 Size: 582 7060 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7642 NotI GC'GGCC_GC Cuts at: 5089 5089 Size: 7642 NspI r_CATG'y Cuts at: 1132 1229 3494 3502 3815 4838 5000 6545 1132 Size: 97 2265 8 313 1023 162 1545 2229 Fragments arranged by size: 2265 2229 1545 1023 313 162 97 8 PasI CC'CwG_GG Cuts at: 1348 2684 3847 1348 Size: 1336 1163 5143 Fragments arranged by size: 5143 1336 1163 PciI A'CATG_T Cuts at: 3498 6541 3498 Size: 3043 4599 PflMI CCAn_nnn'nTGG Cuts at: 1108 3910 4140 1108 Size: 2802 230 4610 Fragments arranged by size: 4610 2802 230 PfoI T'CCnGG_A Cuts at: 3127 3127 Size: 7642 PmeI GTTT'AAAC Cuts at: 4946 4946 Size: 7642 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5711 5743 7224 7256 5711 Size: 32 1481 32 6097 Fragments arranged by size: 6097 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2791 3892 2791 Size: 1101 6541 PshAI GACnn'nnGTC Cuts at: 1613 3329 1613 Size: 1716 5926 PsiI TTA'TAA Cuts at: 5212 5212 Size: 7642 PspOMI G'GGCC_C Cuts at: 3861 4357 3861 Size: 496 7146 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4679 4711 4679 Size: 32 7610 PstI C_TGCA'G Cuts at: 830 2102 2535 2625 3012 3099 3460 3691 4733 Size: 1272 433 90 387 87 361 231 1042 Cuts at: 4733 4994 830 Size: 261 3478 Fragments arranged by size: 3478 1272 1042 433 387 361 261 231 90 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7642 PvuII CAG'CTG Cuts at: 2987 3058 3686 4626 2987 Size: 71 628 940 6003 Fragments arranged by size: 6003 940 628 71 SacII CC_GC'GG Cuts at: 2018 2018 Size: 7642 SalI G'TCGA_C Cuts at: 1936 4984 1936 Size: 3048 4594 SbfI CC_TGCA'GG Cuts at: 4994 4994 Size: 7642 ScaI AGT'ACT Cuts at: 5890 7602 5890 Size: 1712 5930 SexAI A'CCwGG_T Cuts at: 2404 4518 2404 Size: 2114 5528 SfcI C'TryA_G Cuts at: 826 1045 2098 2531 2621 3008 3095 3456 3687 Size: 219 1053 433 90 387 87 361 231 Cuts at: 3687 4729 4990 6125 6806 6997 826 Size: 1042 261 1135 681 191 1471 Fragments arranged by size: 1471 1135 1053 1042 681 433 387 361 261 231 219 191 90 87 SfoI GGC'GCC Cuts at: 2012 2012 Size: 7642 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7642 SmaI CCC'GGG Cuts at: 1872 2091 4969 1872 Size: 219 2878 4545 Fragments arranged by size: 4545 2878 219 SmlI C'TyrA_G Cuts at: 820 1017 1943 4925 5746 6647 6909 820 Size: 197 926 2982 821 901 262 1553 Fragments arranged by size: 2982 1553 926 901 821 262 197 SnaBI TAC'GTA Cuts at: 493 5433 493 Size: 4940 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7642 SphI G_CATG'C Cuts at: 1132 3815 5000 1132 Size: 2683 1185 3774 Fragments arranged by size: 3774 2683 1185 SspI AAT'ATT Cuts at: 5 52 5566 5 Size: 47 5514 2081 Fragments arranged by size: 5514 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7642 StyI C'CwwG_G Cuts at: 513 1065 2281 2372 2712 2751 2885 3108 3865 Size: 552 1216 91 340 39 134 223 757 Cuts at: 3865 4134 5081 513 Size: 269 947 3074 Fragments arranged by size: 3074 1216 947 757 552 340 269 223 134 91 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2324 2410 2881 3736 5669 5686 5839 Size: 1255 744 86 471 855 1933 17 153 Cuts at: 5839 6024 6469 325 Size: 185 445 1498 Fragments arranged by size: 1933 1498 1255 855 744 471 445 185 153 86 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5888 7600 96 Size: 274 80 33 51 1443 3911 1712 138 Fragments arranged by size: 3911 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2680 2712 7602 7634 2680 Size: 32 4890 32 2688 Fragments arranged by size: 4890 2688 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2406 4775 1316 Size: 1090 2369 4183 Fragments arranged by size: 4183 2369 1090 XbaI T'CTAG_A Cuts at: 4978 4978 Size: 7642 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7642 XmaI C'CCGG_G Cuts at: 1870 2089 4967 1870 Size: 219 2878 4545 Fragments arranged by size: 4545 2878 219 XmnI GAAnn'nnTTC Cuts at: 5771 5771 Size: 7642 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5450 276 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI