Restriction map for pFN21AB8541
 (Linear) (Six-base) MAPSORT of: KIBB8541.seq  Check: 7213  from: 1  to: 2937



KIBB8541.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     569     813    1257    1744    2114    2937 
   Size:        569     244     444     487     370     823

  Fragments arranged by size: 

                 823     569     487     444     370     244
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     714     734    2278    2587    2937 
   Size:        714      20    1544     309     350

  Fragments arranged by size: 

                1544     714     350     309      20
 
AflIII A'CryG_T

Cuts at:      0    1493    2937 
   Size:       1493    1444
 
AleI CACnn'nnGTG

Cuts at:      0    1741    2726    2937 
   Size:       1741     985     211

  Fragments arranged by size: 

                1741     985     211
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1010    2937 
   Size:       1010    1927
 
AlwNI CAG_nnn'CTG

Cuts at:      0     137    1011    1098    1572    1890    2937 
   Size:        137     874      87     474     318    1047

  Fragments arranged by size: 

                1047     874     474     318     137      87
 
ApaI G_GGCC'C

Cuts at:      0    1860    2356    2937 
   Size:       1860     496     581

  Fragments arranged by size: 

                1860     581     496
 
ApaLI G'TGCA_C

Cuts at:      0    1489    2719    2937 
   Size:       1489    1230     218

  Fragments arranged by size: 

                1489    1230     218
 
AvaI C'yCGr_G

Cuts at:      0      28      84    2937 
   Size:         28      56    2853

  Fragments arranged by size: 

                2853      56      28
 
BamHI G'GATC_C

Cuts at:      0      34    2937 
   Size:         34    2903
 
BanI G'GyrC_C

Cuts at:      0       5     387     876    1269    2482    2749    2937 
   Size:          5     382     489     393    1213     267     188

  Fragments arranged by size: 

                1213     489     393     382     267     188       5
 
BanII G_rGCy'C

Cuts at:      0     362     676    1640    1860    2356    2937 
   Size:        362     314     964     220     496     581

  Fragments arranged by size: 

                 964     581     496     362     314     220
 
BbeI G_GCGC'C

Cuts at:      0       9    2937 
   Size:          9    2928
 
BbvCI CC'TCA_GC

Cuts at:      0    1386    2617    2937 
   Size:       1386    1231     320

  Fragments arranged by size: 

                1386    1231     320
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2305    2339    2397    2431    2937 
   Size:       2305      34      58      34     506

  Fragments arranged by size: 

                2305     506      58      34      34
 
BclI T'GATC_A

Cuts at:      0    1428    2937 
   Size:       1428    1509
 
BglI GCCn_nnn'nGGC

Cuts at:      0      89    1352    2937 
   Size:         89    1263    1585

  Fragments arranged by size: 

                1585    1263      89
 
BlpI GC'TnA_GC

Cuts at:      0      73     533    1481    2937 
   Size:         73     460     948    1456

  Fragments arranged by size: 

                1456     948     460      73
 
Bme1580I G_kGCm'C

Cuts at:      0     111    1493    1860    2356    2638    2723    2937 
   Size:        111    1382     367     496     282      85     214

  Fragments arranged by size: 

                1382     496     367     282     214     111      85
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     226     300    1360    1971    2937 
   Size:        226      74    1060     611     966

  Fragments arranged by size: 

                1060     966     611     226      74
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1086    2502    2937 
   Size:       1086    1416     435

  Fragments arranged by size: 

                1416    1086     435
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     642    1236    1489    2845    2937 
   Size:        431     211     594     253    1356      92

  Fragments arranged by size: 

                1356     594     431     253     211      92
 
Bpu10I CC'TnA_GC

Cuts at:      0    1061    1386    1441    2617    2937 
   Size:       1061     325      55    1176     320

  Fragments arranged by size: 

                1176    1061     325     320      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2905    2937 
   Size:       2905      32
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31      49     390     669    2937 
   Size:         31      18     341     279    2268

  Fragments arranged by size: 

                2268     341     279      31      18
 
BsaAI yAC'GTr

Cuts at:      0    2499    2937 
   Size:       2499     438
 
BsaBI GATnn'nnATC

Cuts at:      0    2809    2937 
   Size:       2809     128
 
BsaHI Gr'CG_yC

Cuts at:      0       6    2937 
   Size:          6    2931
 
BsaWI w'CCGG_w

Cuts at:      0    1944    2937 
   Size:       1944     993
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1118    1148    2937 
   Size:       1118      30    1789

  Fragments arranged by size: 

                1789    1118      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     501    1275    1687    2053    2937 
   Size:         46     455     774     412     366     884

  Fragments arranged by size: 

                 884     774     455     412     366      46
 
BseYI C'CCAG_C

Cuts at:      0     458     499     809     862     978    1395    1762    2005 
   Size:        458      41     310      53     116     417     367     243

Cuts at:   2005    2937 
   Size:        932

  Fragments arranged by size: 

                 932     458     417     367     310     243     116      53
                  41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1130    2391    2596    2937 
   Size:       1006     124    1261     205     341

  Fragments arranged by size: 

                1261    1006     341     205     124
 
BsiHKAI G_wGCw'C

Cuts at:      0     260     560    1493    2528    2723    2937 
   Size:        260     300     933    1035     195     214

  Fragments arranged by size: 

                1035     933     300     260     214     195
 
BsmI GAATG_Cn'

Cuts at:      0    2916    2937 
   Size:       2916      21
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2581    2937 
   Size:       2581     356
 
Bsp1286I G_dGCh'C

Cuts at:      0     111     260     362     560     676    1493    1640    1860 
   Size:        111     149     102     198     116     817     147     220

Cuts at:   1860    2356    2528    2638    2723    2937 
   Size:        496     172     110      85     214

  Fragments arranged by size: 

                 817     496     220     214     198     172     149     147
                 116     111     110     102      85
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     569     615     813    1257    1744    2114    2937 
   Size:        569      46     198     444     487     370     823

  Fragments arranged by size: 

                 823     569     487     444     370     198      46
 
BsrBI CCG'CTC

Cuts at:      0    2402    2702    2861    2937 
   Size:       2402     300     159      76

  Fragments arranged by size: 

                2402     300     159      76
 
BsrFI r'CCGG_y

Cuts at:      0    1173    2043    2213    2625    2640    2746    2937 
   Size:       1173     870     170     412      15     106     191

  Fragments arranged by size: 

                1173     870     412     191     170     106      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     137     564    1098    2937 
   Size:        137     427     534    1839

  Fragments arranged by size: 

                1839     534     427     137
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     640    2848    2937 
   Size:        640    2208      89

  Fragments arranged by size: 

                2208     640      89
 
BstYI r'GATC_y

Cuts at:      0      34    1472    2937 
   Size:         34    1438    1465

  Fragments arranged by size: 

                1465    1438      34
 
Bsu36I CC'TnA_GG

Cuts at:      0    1500    1850    2937 
   Size:       1500     350    1087

  Fragments arranged by size: 

                1500    1087     350
 
BtgI C'CryG_G

Cuts at:      0      10     111    1367    1860    2069    2894    2937 
   Size:         10     101    1256     493     209     825      43

  Fragments arranged by size: 

                1256     825     493     209     101      43      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2355    2796    2937 
   Size:       2355     441     141

  Fragments arranged by size: 

                2355     441     141
 
BtsI GCAGTG_nn'

Cuts at:      0     187    1235    1971    2461    2721    2733    2937 
   Size:        187    1048     736     490     260      12     204

  Fragments arranged by size: 

                1048     736     490     260     204     187      12
 
EaeI y'GGCC_r

Cuts at:      0     771    1013    1907    2470    2623    2937 
   Size:        771     242     894     563     153     314

  Fragments arranged by size: 

                 894     771     563     314     242     153
 
EarI CTCTTCn'nnn_

Cuts at:      0     733    2937 
   Size:        733    2204
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     435    2914    2937 
   Size:        435    2479      23

  Fragments arranged by size: 

                2479     435      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     642     714     734    1236    1489    2278    2587 
   Size:        431     211      72      20     502     253     789     309

Cuts at:   2587    2845    2937 
   Size:        258      92

  Fragments arranged by size: 

                 789     502     431     309     258     253     211      92
                  72      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     706    2128    2937 
   Size:        706    1422     809

  Fragments arranged by size: 

                1422     809     706
 
EcoO109I rG'GnC_Cy

Cuts at:      0     786     796    1846    1856    1887    2142    2352    2925 
   Size:        786      10    1050      10      31     255     210     573

Cuts at:   2925    2937 
   Size:         12

  Fragments arranged by size: 

                1050     786     573     255     210      31      12      10
                  10
 
EcoRV GAT'ATC

Cuts at:      0    1152    2937 
   Size:       1152    1785
 
FspI TGC'GCA

Cuts at:      0    1703    2937 
   Size:       1703    1234
 
HaeII r_GCGC'y

Cuts at:      0       9     101    1833    2937 
   Size:          9      92    1732    1104

  Fragments arranged by size: 

                1732    1104      92       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     387     419    1136    1168    1523    1555    2937 
   Size:        387      32     717      32     355      32    1382

  Fragments arranged by size: 

                1382     717     387     355      32      32      32
 
KasI G'GCGC_C

Cuts at:      0       5    2937 
   Size:          5    2932
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     285    1122    2501    2937 
   Size:        264      21     837    1379     436

  Fragments arranged by size: 

                1379     837     436     264      21
 
MscI TGG'CCA

Cuts at:      0     773    1909    2472    2937 
   Size:        773    1136     563     465

  Fragments arranged by size: 

                1136     773     563     465
 
MslI CAynn'nnrTG

Cuts at:      0    1522    1741    2419    2501    2716    2726    2767    2834 
   Size:       1522     219     678      82     215      10      41      67

Cuts at:   2834    2937 
   Size:        103

  Fragments arranged by size: 

                1522     678     219     215     103      82      67      41
                  10
 
MspA1I CmG'CkG

Cuts at:      0      12     982    1018    1053    1681    2358    2433    2621 
   Size:         12     970      36      35     628     677      75     188

Cuts at:   2621    2844    2937 
   Size:        223      93

  Fragments arranged by size: 

                 970     677     628     223     188      93      75      36
                  35      12
 
NaeI GCC'GGC

Cuts at:      0    2045    2627    2937 
   Size:       2045     582     310

  Fragments arranged by size: 

                2045     582     310
 
NarI GG'CG_CC

Cuts at:      0       6    2937 
   Size:          6    2931
 
NcoI C'CATG_G

Cuts at:      0    1860    2937 
   Size:       1860    1077
 
NgoMIV G'CCGG_C

Cuts at:      0    2043    2625    2937 
   Size:       2043     582     312

  Fragments arranged by size: 

                2043     582     312
 
NspI r_CATG'y

Cuts at:      0    1489    1497    1810    2833    2937 
   Size:       1489       8     313    1023     104

  Fragments arranged by size: 

                1489    1023     313     104       8
 
PasI CC'CwG_GG

Cuts at:      0     679    1842    2937 
   Size:        679    1163    1095

  Fragments arranged by size: 

                1163    1095     679
 
PciI A'CATG_T

Cuts at:      0    1493    2937 
   Size:       1493    1444
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1905    2135    2937 
   Size:       1905     230     802

  Fragments arranged by size: 

                1905     802     230
 
PfoI T'CCnGG_A

Cuts at:      0    1122    2937 
   Size:       1122    1815
 
PpuMI rG'GwC_Cy

Cuts at:      0     786    1887    2937 
   Size:        786    1101    1050

  Fragments arranged by size: 

                1101    1050     786
 
PshAI GACnn'nnGTC

Cuts at:      0    1324    2937 
   Size:       1324    1613
 
PspOMI G'GGCC_C

Cuts at:      0    1856    2352    2937 
   Size:       1856     496     585

  Fragments arranged by size: 

                1856     585     496
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2674    2706    2937 
   Size:       2674      32     231

  Fragments arranged by size: 

                2674     231      32
 
PstI C_TGCA'G

Cuts at:      0      97     530     620    1007    1094    1455    1686    2728 
   Size:         97     433      90     387      87     361     231    1042

Cuts at:   2728    2937 
   Size:        209

  Fragments arranged by size: 

                1042     433     387     361     231     209      97      90
                  87
 
PvuII CAG'CTG

Cuts at:      0     982    1053    1681    2621    2937 
   Size:        982      71     628     940     316

  Fragments arranged by size: 

                 982     940     628     316      71
 
SacII CC_GC'GG

Cuts at:      0      13    2937 
   Size:         13    2924
 
SexAI A'CCwGG_T

Cuts at:      0     399    2513    2937 
   Size:        399    2114     424

  Fragments arranged by size: 

                2114     424     399
 
SfcI C'TryA_G

Cuts at:      0      93     526     616    1003    1090    1451    1682    2724 
   Size:         93     433      90     387      87     361     231    1042

Cuts at:   2724    2937 
   Size:        213

  Fragments arranged by size: 

                1042     433     387     361     231     213      93      90
                  87
 
SfoI GGC'GCC

Cuts at:      0       7    2937 
   Size:          7    2930
 
SmaI CCC'GGG

Cuts at:      0      86    2937 
   Size:         86    2851
 
SmlI C'TyrA_G

Cuts at:      0    2920    2937 
   Size:       2920      17
 
SphI G_CATG'C

Cuts at:      0    1810    2937 
   Size:       1810    1127
 
StyI C'CwwG_G

Cuts at:      0     276     367     707     746     880    1103    1860    2129 
   Size:        276      91     340      39     134     223     757     269

Cuts at:   2129    2937 
   Size:        808

  Fragments arranged by size: 

                 808     757     340     276     269     223     134      91
                  39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     319     405     876    1731    2937 
   Size:        319      86     471     855    1206

  Fragments arranged by size: 

                1206     855     471     319      86
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     675     707    2937 
   Size:        675      32    2230

  Fragments arranged by size: 

                2230     675      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     401    2770    2937 
   Size:        401    2369     167

  Fragments arranged by size: 

                2369     401     167
 
XmaI C'CCGG_G

Cuts at:      0      84    2937 
   Size:         84    2853
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI
     BglI     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FspI    HaeII
    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI    PpuMI
    PshAI   PspOMI     PsrI     PstI    PvuII    SacII    SexAI     SfcI
     SfoI     SmaI     SmlI     SphI     StyI    TaqII     TstI  Tth111I
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI
    BciVI    BfrBI    BglII    BmgBI     BmtI    BsiEI    BsiWI    BspEI
    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRI
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PmeI
     PmlI     PpiI     PsiI    PspXI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TatI     XbaI     XcmI
     XhoI     XmnI     ZraI
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