(Linear) (Six-base) MAPSORT of: KIBB8541.seq Check: 7213 from: 1 to: 2937 KIBB8541.orf With 182 enzymes: SgfI * July 3, 2012 16:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 569 813 1257 1744 2114 2937 Size: 569 244 444 487 370 823 Fragments arranged by size: 823 569 487 444 370 244 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 714 734 2278 2587 2937 Size: 714 20 1544 309 350 Fragments arranged by size: 1544 714 350 309 20 AflIII A'CryG_T Cuts at: 0 1493 2937 Size: 1493 1444 AleI CACnn'nnGTG Cuts at: 0 1741 2726 2937 Size: 1741 985 211 Fragments arranged by size: 1741 985 211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1010 2937 Size: 1010 1927 AlwNI CAG_nnn'CTG Cuts at: 0 137 1011 1098 1572 1890 2937 Size: 137 874 87 474 318 1047 Fragments arranged by size: 1047 874 474 318 137 87 ApaI G_GGCC'C Cuts at: 0 1860 2356 2937 Size: 1860 496 581 Fragments arranged by size: 1860 581 496 ApaLI G'TGCA_C Cuts at: 0 1489 2719 2937 Size: 1489 1230 218 Fragments arranged by size: 1489 1230 218 AvaI C'yCGr_G Cuts at: 0 28 84 2937 Size: 28 56 2853 Fragments arranged by size: 2853 56 28 BamHI G'GATC_C Cuts at: 0 34 2937 Size: 34 2903 BanI G'GyrC_C Cuts at: 0 5 387 876 1269 2482 2749 2937 Size: 5 382 489 393 1213 267 188 Fragments arranged by size: 1213 489 393 382 267 188 5 BanII G_rGCy'C Cuts at: 0 362 676 1640 1860 2356 2937 Size: 362 314 964 220 496 581 Fragments arranged by size: 964 581 496 362 314 220 BbeI G_GCGC'C Cuts at: 0 9 2937 Size: 9 2928 BbvCI CC'TCA_GC Cuts at: 0 1386 2617 2937 Size: 1386 1231 320 Fragments arranged by size: 1386 1231 320 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2305 2339 2397 2431 2937 Size: 2305 34 58 34 506 Fragments arranged by size: 2305 506 58 34 34 BclI T'GATC_A Cuts at: 0 1428 2937 Size: 1428 1509 BglI GCCn_nnn'nGGC Cuts at: 0 89 1352 2937 Size: 89 1263 1585 Fragments arranged by size: 1585 1263 89 BlpI GC'TnA_GC Cuts at: 0 73 533 1481 2937 Size: 73 460 948 1456 Fragments arranged by size: 1456 948 460 73 Bme1580I G_kGCm'C Cuts at: 0 111 1493 1860 2356 2638 2723 2937 Size: 111 1382 367 496 282 85 214 Fragments arranged by size: 1382 496 367 282 214 111 85 BmrI ACTGGGnnnn_n' Cuts at: 0 226 300 1360 1971 2937 Size: 226 74 1060 611 966 Fragments arranged by size: 1060 966 611 226 74 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1086 2502 2937 Size: 1086 1416 435 Fragments arranged by size: 1416 1086 435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 431 642 1236 1489 2845 2937 Size: 431 211 594 253 1356 92 Fragments arranged by size: 1356 594 431 253 211 92 Bpu10I CC'TnA_GC Cuts at: 0 1061 1386 1441 2617 2937 Size: 1061 325 55 1176 320 Fragments arranged by size: 1176 1061 325 320 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2905 2937 Size: 2905 32 BsaI GGTCTCn'nnnn_ Cuts at: 0 31 49 390 669 2937 Size: 31 18 341 279 2268 Fragments arranged by size: 2268 341 279 31 18 BsaAI yAC'GTr Cuts at: 0 2499 2937 Size: 2499 438 BsaBI GATnn'nnATC Cuts at: 0 2809 2937 Size: 2809 128 BsaHI Gr'CG_yC Cuts at: 0 6 2937 Size: 6 2931 BsaWI w'CCGG_w Cuts at: 0 1944 2937 Size: 1944 993 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1118 1148 2937 Size: 1118 30 1789 Fragments arranged by size: 1789 1118 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 501 1275 1687 2053 2937 Size: 46 455 774 412 366 884 Fragments arranged by size: 884 774 455 412 366 46 BseYI C'CCAG_C Cuts at: 0 458 499 809 862 978 1395 1762 2005 Size: 458 41 310 53 116 417 367 243 Cuts at: 2005 2937 Size: 932 Fragments arranged by size: 932 458 417 367 310 243 116 53 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1006 1130 2391 2596 2937 Size: 1006 124 1261 205 341 Fragments arranged by size: 1261 1006 341 205 124 BsiHKAI G_wGCw'C Cuts at: 0 260 560 1493 2528 2723 2937 Size: 260 300 933 1035 195 214 Fragments arranged by size: 1035 933 300 260 214 195 BsmI GAATG_Cn' Cuts at: 0 2916 2937 Size: 2916 21 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2581 2937 Size: 2581 356 Bsp1286I G_dGCh'C Cuts at: 0 111 260 362 560 676 1493 1640 1860 Size: 111 149 102 198 116 817 147 220 Cuts at: 1860 2356 2528 2638 2723 2937 Size: 496 172 110 85 214 Fragments arranged by size: 817 496 220 214 198 172 149 147 116 111 110 102 85 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 569 615 813 1257 1744 2114 2937 Size: 569 46 198 444 487 370 823 Fragments arranged by size: 823 569 487 444 370 198 46 BsrBI CCG'CTC Cuts at: 0 2402 2702 2861 2937 Size: 2402 300 159 76 Fragments arranged by size: 2402 300 159 76 BsrFI r'CCGG_y Cuts at: 0 1173 2043 2213 2625 2640 2746 2937 Size: 1173 870 170 412 15 106 191 Fragments arranged by size: 1173 870 412 191 170 106 15 BstAPI GCAn_nnn'nTGC Cuts at: 0 137 564 1098 2937 Size: 137 427 534 1839 Fragments arranged by size: 1839 534 427 137 BstXI CCAn_nnnn'nTGG Cuts at: 0 640 2848 2937 Size: 640 2208 89 Fragments arranged by size: 2208 640 89 BstYI r'GATC_y Cuts at: 0 34 1472 2937 Size: 34 1438 1465 Fragments arranged by size: 1465 1438 34 Bsu36I CC'TnA_GG Cuts at: 0 1500 1850 2937 Size: 1500 350 1087 Fragments arranged by size: 1500 1087 350 BtgI C'CryG_G Cuts at: 0 10 111 1367 1860 2069 2894 2937 Size: 10 101 1256 493 209 825 43 Fragments arranged by size: 1256 825 493 209 101 43 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2355 2796 2937 Size: 2355 441 141 Fragments arranged by size: 2355 441 141 BtsI GCAGTG_nn' Cuts at: 0 187 1235 1971 2461 2721 2733 2937 Size: 187 1048 736 490 260 12 204 Fragments arranged by size: 1048 736 490 260 204 187 12 EaeI y'GGCC_r Cuts at: 0 771 1013 1907 2470 2623 2937 Size: 771 242 894 563 153 314 Fragments arranged by size: 894 771 563 314 242 153 EarI CTCTTCn'nnn_ Cuts at: 0 733 2937 Size: 733 2204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 435 2914 2937 Size: 435 2479 23 Fragments arranged by size: 2479 435 23 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 431 642 714 734 1236 1489 2278 2587 Size: 431 211 72 20 502 253 789 309 Cuts at: 2587 2845 2937 Size: 258 92 Fragments arranged by size: 789 502 431 309 258 253 211 92 72 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 706 2128 2937 Size: 706 1422 809 Fragments arranged by size: 1422 809 706 EcoO109I rG'GnC_Cy Cuts at: 0 786 796 1846 1856 1887 2142 2352 2925 Size: 786 10 1050 10 31 255 210 573 Cuts at: 2925 2937 Size: 12 Fragments arranged by size: 1050 786 573 255 210 31 12 10 10 EcoRV GAT'ATC Cuts at: 0 1152 2937 Size: 1152 1785 FspI TGC'GCA Cuts at: 0 1703 2937 Size: 1703 1234 HaeII r_GCGC'y Cuts at: 0 9 101 1833 2937 Size: 9 92 1732 1104 Fragments arranged by size: 1732 1104 92 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 387 419 1136 1168 1523 1555 2937 Size: 387 32 717 32 355 32 1382 Fragments arranged by size: 1382 717 387 355 32 32 32 KasI G'GCGC_C Cuts at: 0 5 2937 Size: 5 2932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 264 285 1122 2501 2937 Size: 264 21 837 1379 436 Fragments arranged by size: 1379 837 436 264 21 MscI TGG'CCA Cuts at: 0 773 1909 2472 2937 Size: 773 1136 563 465 Fragments arranged by size: 1136 773 563 465 MslI CAynn'nnrTG Cuts at: 0 1522 1741 2419 2501 2716 2726 2767 2834 Size: 1522 219 678 82 215 10 41 67 Cuts at: 2834 2937 Size: 103 Fragments arranged by size: 1522 678 219 215 103 82 67 41 10 MspA1I CmG'CkG Cuts at: 0 12 982 1018 1053 1681 2358 2433 2621 Size: 12 970 36 35 628 677 75 188 Cuts at: 2621 2844 2937 Size: 223 93 Fragments arranged by size: 970 677 628 223 188 93 75 36 35 12 NaeI GCC'GGC Cuts at: 0 2045 2627 2937 Size: 2045 582 310 Fragments arranged by size: 2045 582 310 NarI GG'CG_CC Cuts at: 0 6 2937 Size: 6 2931 NcoI C'CATG_G Cuts at: 0 1860 2937 Size: 1860 1077 NgoMIV G'CCGG_C Cuts at: 0 2043 2625 2937 Size: 2043 582 312 Fragments arranged by size: 2043 582 312 NspI r_CATG'y Cuts at: 0 1489 1497 1810 2833 2937 Size: 1489 8 313 1023 104 Fragments arranged by size: 1489 1023 313 104 8 PasI CC'CwG_GG Cuts at: 0 679 1842 2937 Size: 679 1163 1095 Fragments arranged by size: 1163 1095 679 PciI A'CATG_T Cuts at: 0 1493 2937 Size: 1493 1444 PflMI CCAn_nnn'nTGG Cuts at: 0 1905 2135 2937 Size: 1905 230 802 Fragments arranged by size: 1905 802 230 PfoI T'CCnGG_A Cuts at: 0 1122 2937 Size: 1122 1815 PpuMI rG'GwC_Cy Cuts at: 0 786 1887 2937 Size: 786 1101 1050 Fragments arranged by size: 1101 1050 786 PshAI GACnn'nnGTC Cuts at: 0 1324 2937 Size: 1324 1613 PspOMI G'GGCC_C Cuts at: 0 1856 2352 2937 Size: 1856 496 585 Fragments arranged by size: 1856 585 496 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2674 2706 2937 Size: 2674 32 231 Fragments arranged by size: 2674 231 32 PstI C_TGCA'G Cuts at: 0 97 530 620 1007 1094 1455 1686 2728 Size: 97 433 90 387 87 361 231 1042 Cuts at: 2728 2937 Size: 209 Fragments arranged by size: 1042 433 387 361 231 209 97 90 87 PvuII CAG'CTG Cuts at: 0 982 1053 1681 2621 2937 Size: 982 71 628 940 316 Fragments arranged by size: 982 940 628 316 71 SacII CC_GC'GG Cuts at: 0 13 2937 Size: 13 2924 SexAI A'CCwGG_T Cuts at: 0 399 2513 2937 Size: 399 2114 424 Fragments arranged by size: 2114 424 399 SfcI C'TryA_G Cuts at: 0 93 526 616 1003 1090 1451 1682 2724 Size: 93 433 90 387 87 361 231 1042 Cuts at: 2724 2937 Size: 213 Fragments arranged by size: 1042 433 387 361 231 213 93 90 87 SfoI GGC'GCC Cuts at: 0 7 2937 Size: 7 2930 SmaI CCC'GGG Cuts at: 0 86 2937 Size: 86 2851 SmlI C'TyrA_G Cuts at: 0 2920 2937 Size: 2920 17 SphI G_CATG'C Cuts at: 0 1810 2937 Size: 1810 1127 StyI C'CwwG_G Cuts at: 0 276 367 707 746 880 1103 1860 2129 Size: 276 91 340 39 134 223 757 269 Cuts at: 2129 2937 Size: 808 Fragments arranged by size: 808 757 340 276 269 223 134 91 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 319 405 876 1731 2937 Size: 319 86 471 855 1206 Fragments arranged by size: 1206 855 471 319 86 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 675 707 2937 Size: 675 32 2230 Fragments arranged by size: 2230 675 32 Tth111I GACn'n_nGTC Cuts at: 0 401 2770 2937 Size: 401 2369 167 Fragments arranged by size: 2369 401 167 XmaI C'CCGG_G Cuts at: 0 84 2937 Size: 84 2853 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PsrI PstI PvuII SacII SexAI SfcI SfoI SmaI SmlI SphI StyI TaqII TstI Tth111I XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI BfrBI BglII BmgBI BmtI BsiEI BsiWI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PspXI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI XcmI XhoI XmnI ZraI