(Circular) (Six-base) MAPSORT of: pFN21AE1829.seq Check: 9392 from: 1 to: 5746 pFN21AE1829 5746 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2713 3089 1937 Size: 776 376 4594 Fragments arranged by size: 4594 776 376 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 2908 3873 4246 1563 Size: 1345 965 373 3063 Fragments arranged by size: 3063 1345 965 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2361 2513 3809 5193 5461 1632 Size: 729 152 1296 1384 268 1917 Fragments arranged by size: 1917 1384 1296 729 268 152 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 2576 1166 Size: 1410 4336 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 2899 3489 5061 2899 Size: 590 1572 3584 Fragments arranged by size: 3584 1572 590 ApaI G_GGCC'C Cuts at: 2050 2509 2671 2050 Size: 459 162 5125 Fragments arranged by size: 5125 459 162 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 3071 1870 Size: 73 1128 4545 Fragments arranged by size: 4545 1128 73 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2706 3039 3067 4522 618 Size: 325 217 627 919 333 28 1455 1842 Fragments arranged by size: 1842 1455 919 627 333 325 217 28 BanII G_rGCy'C Cuts at: 1391 2050 2283 2327 2509 2660 2671 2792 3065 Size: 659 233 44 182 151 11 121 273 Cuts at: 3065 1391 Size: 4072 Fragments arranged by size: 4072 659 273 233 182 151 121 44 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2960 928 Size: 971 1061 3714 Fragments arranged by size: 3714 1061 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3937 3971 1903 Size: 34 50 34 1916 34 3678 Fragments arranged by size: 3678 1916 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 1548 Size: 5746 BfrBI ATG'CAT Cuts at: 2885 2885 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 4357 136 Size: 107 122 71 631 605 373 2312 1525 Fragments arranged by size: 2312 1525 631 605 373 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 2138 2277 2966 3163 2138 Size: 139 689 197 4721 Fragments arranged by size: 4721 689 197 139 Bme1580I G_kGCm'C Cuts at: 1790 2050 2509 2671 2947 3807 4963 1790 Size: 260 459 162 276 860 1156 2573 Fragments arranged by size: 2573 1156 860 459 276 260 162 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2942 3477 4435 Size: 416 53 83 399 24 1525 535 958 Cuts at: 4435 442 Size: 1753 Fragments arranged by size: 1753 1525 958 535 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2531 2556 4406 1239 Size: 800 492 25 1850 2579 Fragments arranged by size: 2579 1850 800 492 25 Bpu10I CC'TnA_GC Cuts at: 2249 2249 Size: 5746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5521 Fragments arranged by size: 5521 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2876 1186 Size: 1690 4056 BseYI C'CCAG_C Cuts at: 2623 4949 5445 2623 Size: 2326 496 2924 Fragments arranged by size: 2924 2326 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2018 1163 Size: 573 282 4891 Fragments arranged by size: 4891 573 282 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 2583 2660 3000 3807 3892 4963 2283 Size: 300 77 340 807 85 1071 3066 Fragments arranged by size: 3066 1071 807 340 300 85 77 BsiWI C'GTAC_G Cuts at: 2093 2093 Size: 5746 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2283 2327 2509 2583 2660 2671 Size: 399 260 233 44 182 74 77 11 Cuts at: 2671 2792 2947 3000 3065 3807 3892 4963 1391 Size: 121 155 53 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 260 233 182 155 121 85 77 74 65 53 44 11 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 844 Size: 2257 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2395 3633 1394 Size: 429 572 1238 3507 Fragments arranged by size: 3507 1238 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4241 4415 65 Size: 1183 2993 174 1396 Fragments arranged by size: 2993 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 2434 2467 1184 Size: 1250 33 4463 Fragments arranged by size: 4463 1250 33 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2305 Size: 5746 BstYI r'GATC_y Cuts at: 1972 2263 3076 3443 3828 3845 5297 5383 5395 Size: 291 813 367 385 17 1452 86 12 Cuts at: 5395 5741 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 813 385 367 346 291 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2549 513 Size: 552 1484 3710 Fragments arranged by size: 3710 1484 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 2014 2079 2211 3262 4056 4076 2014 Size: 65 132 1051 794 20 3684 Fragments arranged by size: 3684 1051 794 132 65 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2546 3193 Size: 54 1425 50 275 18 246 470 647 Cuts at: 3193 4082 5711 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 647 470 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3676 1353 Size: 2323 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2281 2658 2281 Size: 377 5369 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2361 2513 2531 2556 3809 4406 Size: 393 407 322 152 18 25 1253 597 Cuts at: 4406 5193 5461 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1253 787 597 407 393 322 268 152 25 18 EcoNI CCTnn'n_nnAGG Cuts at: 2054 2606 2054 Size: 552 5194 EcoO109I rG'GnC_Cy Cuts at: 2047 2505 2506 2047 Size: 458 1 5287 Fragments arranged by size: 5287 458 1 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 2500 4893 1678 Size: 822 2393 2531 Fragments arranged by size: 2531 2393 822 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2461 2493 2695 2727 4387 Size: 32 135 32 889 32 202 32 1660 Cuts at: 4387 4419 4461 4493 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1660 889 202 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2714 2738 3090 3336 669 Size: 988 281 776 24 352 246 3079 Fragments arranged by size: 3079 988 776 352 281 246 24 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2622 2726 2897 4860 5044 1305 Size: 1317 104 171 1963 184 2007 Fragments arranged by size: 2007 1963 1317 184 171 104 MscI TGG'CCA Cuts at: 10 64 1489 1832 2078 10 Size: 54 1425 343 246 3678 Fragments arranged by size: 3678 1425 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2576 3023 3704 4063 4222 518 Size: 648 1410 447 681 359 159 2042 Fragments arranged by size: 2042 1410 681 648 447 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2293 2385 2551 2623 2683 2965 3162 Size: 552 135 92 166 72 60 282 197 Cuts at: 3162 3839 4987 5232 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 282 245 197 166 135 92 72 60 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5194 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NsiI A_TGCA'T Cuts at: 2887 2887 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2137 2974 3104 4649 1132 Size: 97 908 837 130 1545 2229 Fragments arranged by size: 2229 1545 908 837 130 97 PasI CC'CwG_GG Cuts at: 1348 2501 1348 Size: 1153 4593 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PfoI T'CCnGG_A Cuts at: 2792 2792 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2683 2733 1613 Size: 1070 50 4626 Fragments arranged by size: 4626 1070 50 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspOMI G'GGCC_C Cuts at: 2046 2505 2667 2046 Size: 459 162 5125 Fragments arranged by size: 5125 459 162 PstI C_TGCA'G Cuts at: 830 2292 3098 830 Size: 1462 806 3478 Fragments arranged by size: 3478 1462 806 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2293 2385 2623 2683 2293 Size: 92 238 60 5356 Fragments arranged by size: 5356 238 92 60 SacI G_AGCT'C Cuts at: 2283 2660 2283 Size: 377 5369 SacII CC_GC'GG Cuts at: 2552 2552 Size: 5746 SalI G'TCGA_C Cuts at: 1936 2712 3088 1936 Size: 776 376 4594 Fragments arranged by size: 4594 776 376 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 2186 2288 3094 4229 4910 5101 826 Size: 219 1141 102 806 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 806 681 219 191 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3850 4751 5013 820 Size: 197 926 1907 901 262 1553 Fragments arranged by size: 1907 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 2974 3104 1132 Size: 1842 130 3774 Fragments arranged by size: 3774 1842 130 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 2476 1517 Size: 959 4787 StyI C'CwwG_G Cuts at: 513 1065 2445 3185 513 Size: 552 1380 740 3074 Fragments arranged by size: 3074 1380 740 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 3027 3773 3790 3943 4128 4573 Size: 1255 1035 412 746 17 153 185 445 Cuts at: 4573 325 Size: 1498 Fragments arranged by size: 1498 1255 1035 746 445 412 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2814 3992 5704 Size: 274 80 33 51 1443 837 1178 1712 Cuts at: 5704 96 Size: 138 Fragments arranged by size: 1712 1443 1178 837 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5706 5738 5706 Size: 32 5714 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI