################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AclI AfaI AflII AflIII AhdI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BsaAI BsaI BsiWI BsmBI Bsp1407I BspDI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MluI MluNI MscI NarI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 926 TCCGGA T^CCGGA AcsI 1810 GAATTT [GA]^AATT[CT] AgeI 2134 ACCGGT A^CCGGT Alw44I 1684 GTGCAC G^TGCAC AlwNI 1108 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 296 AGCGCT AGC^GCT ApaLI 1684 GTGCAC G^TGCAC ApoI 1810 GAATTT [GA]^AATT[CT] AspI 550 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 568 GGCACC G^G[CT][GA]CC BbsI 1857 GTCTTC GAAGAC(2/6) BclI 1509 TGATCA T^GATCA BfaI 1001 CTAG C^TAG BlpI 1329 GCTAAGC GC^T[ACGT]AGC BpmI 991 CTGGAG CTGGAG(16/14) Bpu1102I 1329 GCTAAGC GC^T[ACGT]AGC BpuAI 1857 GTCTTC GAAGAC(2/6) BsaBI 1605 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 926 TCCGGA T^CCGGA BsgI 375 GTGCAG GTGCAG(16/14) BspEI 926 TCCGGA T^CCGGA BstAPI 1369 GCATCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 292 CCAAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1329 GCTAAGC GC^T[ACGT]AGC DraIII 383 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2114 CCATGG C^C[GA][CT]GG Eco47III 296 AGCGCT AGC^GCT FbaI 1509 TGATCA T^GATCA HaeII 296 AGCGCT [GA]GCGC^[CT] MaeI 1001 CTAG C^TAG MamI 1605 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1502 CAATTG C^AATTG MroI 926 TCCGGA T^CCGGA MslI 690 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1502 CAATTG C^AATTG NaeI 29 GCCGGC GCC^GGC NcoI 2114 CCATGG C^CATGG NdeI 44 CATATG CA^TATG NgoMIV 29 GCCGGC G^CCGGC PflFI 550 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1077 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2134 ACCGGT A^CCGGT PshAI 892 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1206 CTGCAG CTGCA^G PvuI 563 CGATCG CGAT^CG PvuII 2031 CAGCTG CAG^CTG TaqI 562 TCGA T^CGA Tth111I 550 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 562 TCGA T^CGA Van91I 1077 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 151 GACGCC G[GA]^CG[CT]C 341 GACGCC AocI 720 CCTCAGG CC^T[ACGT]AGG 760 CCTGAGG Asp700I 1088 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1489 GAAGTGTTTC BamHI 1240 GGATCC G^GATCC 1567 GGATCC BanII 311 GAGCTC G[GA]GC[CT]^C 356 GAGCTC BsaHI 151 GACGCC G[GA]^CG[CT]C 341 GACGCC BspHI 1274 TCATGA T^CATGA 2171 TCATGA BsrBI 394 CCGCTC CCGCTC(-3/-3) 606 GAGCGG BsrDI 1429 CATTGC GCAATG(2/0) 1536 CATTGC Bsu36I 720 CCTCAGG CC^T[ACGT]AGG 760 CCTGAGG DraI 1066 TTTAAA TTT^AAA 1780 TTTAAA DrdI 839 GACCAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1188 GACTGATGTGTC Eco81I 720 CCTCAGG CC^T[ACGT]AGG 760 CCTGAGG HgaI 151 GACGC GACGC(5/10) 341 GACGC Hin1I 151 GACGCC G[GA]^CG[CT]C 341 GACGCC MaeII 1961 ACGT A^CGT 2108 ACGT RcaI 1274 TCATGA T^CATGA 2171 TCATGA SacI 311 GAGCTC GAGCT^C 356 GAGCTC SfcI 1206 CTGCAG C^T[GA][CT]AG 2163 CTACAG SmaI 15 CCCGGG CCC^GGG 529 CCCGGG TfiI 766 GATTC G^A[AT]TC 1093 GATTC XmaCI 15 CCCGGG C^CCGGG 529 CCCGGG XmaI 15 CCCGGG C^CCGGG 529 CCCGGG XmnI 1088 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1489 GAAGTGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AluI AlwI AspHI AvaI AvaII BbvCI BbvI BcnI BmyI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI SapI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII