################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccIII AcyI AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI BbeI BbrPI BbvCI BcgI BsaHI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BsrBI BssHII BssSI ClaI CpoI DraIII DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I HgaI Hin1I HindIII HpaI KasI KspI MluI MroI NarI NdeI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SpeI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 1511 GGTACC G^GTACC AccII 3316 CGCG CG^CG AclI 544 AACGTT AA^CGTT AocI 3221 CCTCAGG CC^T[ACGT]AGG ApaI 3694 GGGCCC GGGCC^C Asp718I 1511 GGTACC G^GTACC AviII 2404 TGCGCA TGC^GCA AvrII 798 CCTAGG C^CTAGG BbsI 3771 GTCTTC GAAGAC(2/6) BciVI 3799 GGATAC GTATCC(6/5) BclI 1407 TGATCA T^GATCA BglI 3715 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1832 AGATCT A^GATCT BlnI 798 CCTAGG C^CTAGG BpuAI 3771 GTCTTC GAAGAC(2/6) BsaAI 3549 TACGTA [CT]AC^GT[GA] BsaBI 2875 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3226 GGTCTC GGTCTC(1/5) Bsp1407I 2323 TGTACA T^GTACA BsrGI 2323 TGTACA T^GTACA Bst1107I 3546 GTATAC GTA^TAC BstBI 2739 TTCGAA TT^CGAA BstEII 1310 GGTTACC G^GT[ACGT]ACC BstPI 1310 GGTTACC G^GT[ACGT]ACC BstUI 3316 CGCG CG^CG BstXI 2601 CCACTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3546 GTATAC GTA^TAC Bsu36I 3221 CCTCAGG CC^T[ACGT]AGG DraI 42 TTTAAA TTT^AAA DsaI 2561 CCATGG C^C[GA][CT]GG Eco81I 3221 CCTCAGG CC^T[ACGT]AGG EcoNI 129 CCTACTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1310 GGTTACC G^GT[ACGT]ACC FbaI 1407 TGATCA T^GATCA FseI 2413 GGCCGGCC GGCCGG^CC FspI 2404 TGCGCA TGC^GCA HaeII 2421 GGCGCT [GA]GCGC^[CT] HincII 601 GTTGAC GT[CT]^[GA]AC HindII 601 GTTGAC GT[CT]^[GA]AC KpnI 1511 GGTACC GGTAC^C MamI 2875 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 3316 CGCG CG^CG NcoI 2561 CCATGG C^CATGG NheI 2580 GCTAGC G^CTAGC NspV 2739 TTCGAA TT^CGAA PaeR7I 133 CTCGAG C^TCGAG PflMI 2080 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 3677 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 544 AACGTT AA^CGTT PvuI 171 CGATCG CGAT^CG ScaI 162 AGTACT AGT^ACT SfuI 2739 TTCGAA TT^CGAA SnaBI 3549 TACGTA TAC^GTA SphI 3633 GCATGC GCATG^C SspBI 2323 TGTACA T^GTACA SspI 335 AATATT AAT^ATT StuI 3218 AGGCCT AGG^CCT Van91I 2080 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2784 TCTAGA T^CTAGA XhoI 133 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 1629 GTCTAC GT^[AC][GT]AC 3546 GTATAC AflII 1159 CTTAAG C^TTAAG 1528 CTTAAG Alw44I 1957 GTGCAC G^TGCAC 3670 GTGCAC ApaLI 1957 GTGCAC G^TGCAC 3670 GTGCAC Asp700I 1297 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1450 GAATATCTTC BalI 1109 TGGCCA TGG^CCA 2669 TGGCCA BamHI 17 GGATCC G^GATCC 3109 GGATCC BcnI 804 CCGGG CC^[GC]GG 3510 CCCGG BfrI 1159 CTTAAG C^TTAAG 1528 CTTAAG BsmI 2595 GAATGC GAATGC(1/-1) 2217 GCATTC BsrDI 3597 CATTGC GCAATG(2/0) 2545 GCAATG CfoI 2405 GCGC GCG^C 2422 GCGC EagI 2412 CGGCCG C^GGCCG 2416 CGGCCG EclXI 2412 CGGCCG C^GGCCG 2416 CGGCCG Eco52I 2412 CGGCCG C^GGCCG 2416 CGGCCG HhaI 2405 GCGC GCG^C 2422 GCGC HinP1I 2405 GCGC G^CGC 2422 GCGC MfeI 1712 CAATTG C^AATTG 2255 CAATTG MluNI 1109 TGGCCA TGG^CCA 2669 TGGCCA MscI 1109 TGGCCA TGG^CCA 2669 TGGCCA MunI 1712 CAATTG C^AATTG 2255 CAATTG NciI 804 CCGGG CC^[GC]GG 3510 CCCGG SacI 1289 GAGCTC GAGCT^C 3308 GAGCTC XcmI 848 CCAAGGAAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3756 CCAATCCTACATTGG XmnI 1297 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1450 GAATATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NaeI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII