################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BlnI BpmI BsaHI BsaI BseRI BsiEI BsiWI BsmBI BspDI BspHI BspMI BsrBI BsrFI BssHII BssSI BstUI BstXI Bsu36I Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FauI FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MfeI MluI MluNI MscI MslI MunI MvnI NaeI NarI NciI NgoMIV NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflIII 1435 ACATGT A^C[GA][CT]GT AspHI 2441 GAGCAC G[AT]GC[AT]^C BanI 304 GGTGCC G^G[CT][GA]CC BanII 1195 GAGCCC G[GA]GC[CT]^C BbvCI 478 GCTGAGG CCTCAGC(-5/-2) BglII 2055 AGATCT A^GATCT BlpI 278 GCTCAGC GC^T[ACGT]AGC Bpu1102I 278 GCTCAGC GC^T[ACGT]AGC BsaAI 2598 CACGTA [CT]AC^GT[GA] BsaBI 250 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 2441 GAGCAC G[AT]GC[AT]^C BsmI 1452 GAATGC GAATGC(1/-1) BspLU11I 1435 ACATGT A^CATGT BsrDI 1058 GCAATG GCAATG(2/0) BstAPI 589 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1113 TTCGAA TT^CGAA CelII 278 GCTCAGC GC^T[ACGT]AGC CfoI 350 GCGC GCG^C DsaI 1901 CCATGG C^C[GA][CT]GG EcoNI 2446 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1493 GAATTC G^AATTC EcoRV 2043 GATATC GAT^ATC HaeII 349 GGCGCT [GA]GCGC^[CT] HhaI 350 GCGC GCG^C HinP1I 350 GCGC G^CGC HincII 117 GTTAAC GT[CT]^[GA]AC HindII 117 GTTAAC GT[CT]^[GA]AC HpaI 117 GTTAAC GTT^AAC MamI 250 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1901 CCATGG C^CATGG NdeI 2212 CATATG CA^TATG NheI 61 GCTAGC G^CTAGC NspV 1113 TTCGAA TT^CGAA PflMI 1753 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1263 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 977 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1643 CTGCAG CTGCA^G SapI 1192 GAAGAGC GCTCTTC(1/4) SfuI 1113 TTCGAA TT^CGAA SphI 2618 GCATGC GCATG^C Van91I 1753 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 500 TCCGGA T^CCGGA 2547 TCCGGA AlwI 250 GATCC GGATC(4/5) 838 GATCC AseI 232 ATTAAT AT^TAAT 1348 ATTAAT AsnI 232 ATTAAT AT^TAAT 1348 ATTAAT AvaII 1264 GGTCC G^G[AT]CC 1275 GGACC BbsI 556 GAAGAC GAAGAC(2/6) 613 GAAGAC BclI 971 TGATCA T^GATCA 2030 TGATCA BpuAI 556 GAAGAC GAAGAC(2/6) 613 GAAGAC BsaWI 500 TCCGGA [AT]^CCGG[AT] 2547 TCCGGA BseAI 500 TCCGGA T^CCGGA 2547 TCCGGA BsgI 10 CTGCAC GTGCAG(16/14) 144 CTGCAC BsmFI 2345 GGGAC GGGAC(10/14) 1439 GTCCC Bsp1407I 1502 TGTACA T^GTACA 1890 TGTACA BspEI 500 TCCGGA T^CCGGA 2547 TCCGGA BsrGI 1502 TGTACA T^GTACA 1890 TGTACA Bst1107I 826 GTATAC GTA^TAC 1880 GTATAC BstYI 837 AGATCC [GA]^GATC[CT] 2055 AGATCT BstZ17I 826 GTATAC GTA^TAC 1880 GTATAC DraI 364 TTTAAA TTT^AAA 1983 TTTAAA DraII 681 AGGCCCT [GA]G^G[ACGT]CC[CT] 1263 AGGTCCT EcoO109I 681 AGGCCCT [GA]G^G[ACGT]CC[CT] 1263 AGGTCCT FbaI 971 TGATCA T^GATCA 2030 TGATCA MflI 837 AGATCC [GA]^GATC[CT] 2055 AGATCT MroI 500 TCCGGA T^CCGGA 2547 TCCGGA MspA1I 80 CAGCTG C[AC]G^C[GT]G 1726 CAGCTG NlaIV 304 GGTGCC GG[ACGT]^[ACGT]CC 1275 GGACCC PshBI 232 ATTAAT AT^TAAT 1348 ATTAAT PvuII 80 CAGCTG CAG^CTG 1726 CAGCTG ScaI 1851 AGTACT AGT^ACT 2240 AGTACT SspBI 1502 TGTACA T^GTACA 1890 TGTACA XbaI 1371 TCTAGA T^CTAGA 2292 TCTAGA XhoII 837 AGATCC [GA]^GATC[CT] 2055 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccI AcsI AfaI AluI ApoI Asp700I AvaI BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI Cac8I Cfr13I DdeI DpnII EarI EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NspI PaeR7I PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoI XmnI