################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbsI BbvCI BcgI BclI BfrI BlnI BpuAI BseAI BsiWI Bsp1407I BspDI BspEI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AflIII 2081 ACATGT A^C[GA][CT]GT Alw44I 1593 GTGCAC G^TGCAC ApaLI 1593 GTGCAC G^TGCAC Asp700I 2400 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 1435 GGCGCC GGCGC^C BbrPI 1705 CACGTG CAC^GTG BciVI 1026 GGATAC GTATCC(6/5) BfaI 2137 CTAG C^TAG BglII 1856 AGATCT A^GATCT BsiEI 1794 CGACCG CG[GA][CT]^CG BsmBI 2347 CGTCTC CGTCTC(1/5) BsmI 703 GCATTC GAATGC(1/-1) BspHI 1688 TCATGA T^CATGA BspLU11I 2081 ACATGT A^CATGT BsrBI 181 GAGCGG CCGCTC(-3/-3) DsaI 71 CCATGG C^C[GA][CT]GG KasI 1435 GGCGCC G^GCGCC MaeI 2137 CTAG C^TAG NarI 1435 GGCGCC GG^CGCC NcoI 71 CCATGG C^CATGG PaeR7I 1994 CTCGAG C^TCGAG PmaCI 1705 CACGTG CAC^GTG PmlI 1705 CACGTG CAC^GTG PpuMI 275 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 1688 TCATGA T^CATGA SexAI 843 ACCAGGT A^CC[AT]GGT SfoI 1435 GGCGCC GGC^GCC XhoI 1994 CTCGAG C^TCGAG XmnI 2400 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 2163 GAATTT [GA]^AATT[CT] 2440 AAATTT AcyI 1435 GGCGCC G[GA]^CG[CT]C 1729 GACGCC AocI 469 CCTGAGG CC^T[ACGT]AGG 771 CCTCAGG ApoI 2163 GAATTT [GA]^AATT[CT] 2440 AAATTT BglI 205 GCCTCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 942 GCCTCCAGGGC BlpI 522 GCTGAGC GC^T[ACGT]AGC 1698 GCTCAGC Bpu1102I 522 GCTGAGC GC^T[ACGT]AGC 1698 GCTCAGC BsaAI 1705 CACGTG [CT]AC^GT[GA] 1771 TACGTG BsaBI 1002 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1680 GATCAACATC BsaHI 1435 GGCGCC G[GA]^CG[CT]C 1729 GACGCC BsaWI 1398 TCCGGT [AT]^CCGG[AT] 1796 ACCGGA Bsu36I 469 CCTGAGG CC^T[ACGT]AGG 771 CCTCAGG CelII 522 GCTGAGC GC^T[ACGT]AGC 1698 GCTCAGC DraIII 842 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2283 CACACAGTG Eco81I 469 CCTGAGG CC^T[ACGT]AGG 771 CCTCAGG EcoNI 420 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1332 CCTGCAGCAGG Hin1I 1435 GGCGCC G[GA]^CG[CT]C 1729 GACGCC HindIII 1284 AAGCTT A^AGCTT 2327 AAGCTT MamI 1002 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1680 GATCAACATC PflMI 651 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 916 CCAGGGCCTGG PvuII 1097 CAGCTG CAG^CTG 1749 CAGCTG SacI 1195 GAGCTC GAGCT^C 1786 GAGCTC SmlI 1909 CTCAAG C^T[CT][GA]AG 1994 CTCGAG Van91I 651 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 916 CCAGGGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI