(Circular) (Six-base) MAPSORT of: pFN21AE3538.seq Check: 7729 from: 1 to: 7162 pFN21AE3538 7162 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2730 2730 Size: 7162 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4972 278 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4505 1937 Size: 2568 4594 Acc65I G'GTAC_C Cuts at: 4483 4483 Size: 7162 AclI AA'CG_TT Cuts at: 1563 5289 5662 1563 Size: 3726 373 3063 Fragments arranged by size: 3726 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2332 3207 3435 4031 5225 6609 6877 1632 Size: 700 875 228 596 1194 1384 268 1917 Fragments arranged by size: 1917 1384 1194 875 700 596 268 228 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6965 AflIII A'CryG_T Cuts at: 1203 4086 6061 7038 1203 Size: 2883 1975 977 1327 Fragments arranged by size: 2883 1975 1327 977 AgeI A'CCGG_T Cuts at: 5993 5993 Size: 7162 AhdI GACnn_n'nnGTC Cuts at: 5891 5891 Size: 7162 AleI CACnn'nnGTG Cuts at: 1166 2738 2985 1166 Size: 1572 247 5343 Fragments arranged by size: 5343 1572 247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2328 2568 3405 1184 Size: 1144 240 837 4941 Fragments arranged by size: 4941 1144 837 240 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2673 2705 2673 Size: 32 7130 AlwNI CAG_nnn'CTG Cuts at: 2122 2927 3104 4297 4905 6477 2122 Size: 805 177 1193 608 1572 2807 Fragments arranged by size: 2807 1572 1193 805 608 177 ApaI G_GGCC'C Cuts at: 2228 3128 3331 2228 Size: 900 203 6059 Fragments arranged by size: 6059 900 203 ApaLI G'TGCA_C Cuts at: 3598 5219 6375 3598 Size: 1621 1156 4385 Fragments arranged by size: 4385 1621 1156 ApoI r'AATT_y Cuts at: 1493 4168 4445 4471 4697 4849 6958 1493 Size: 2675 277 26 226 152 2109 1697 Fragments arranged by size: 2675 2109 1697 277 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 5716 160 Size: 872 4684 1606 Fragments arranged by size: 4684 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2227 2276 2830 3230 3999 4487 Size: 73 271 13 49 554 400 769 488 Cuts at: 4487 1870 Size: 4545 Fragments arranged by size: 4545 769 554 488 400 271 73 49 13 BamHI G'GATC_C Cuts at: 4492 4859 4492 Size: 367 6795 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 2327 3440 4483 5938 Size: 325 217 627 431 109 1113 1043 1455 Cuts at: 5938 618 Size: 1842 Fragments arranged by size: 1842 1455 1113 1043 627 431 325 217 109 BanII G_rGCy'C Cuts at: 1391 2228 2895 3128 3171 3204 3331 3403 3795 Size: 837 667 233 43 33 127 72 392 Cuts at: 3795 4221 4481 1391 Size: 426 260 4072 Fragments arranged by size: 4072 837 667 426 392 260 233 127 72 43 33 BbeI G_GCGC'C Cuts at: 3444 3444 Size: 7162 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6191 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5353 5387 1903 Size: 34 3416 34 3678 Fragments arranged by size: 3678 3416 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3025 5054 6264 3025 Size: 2029 1210 3923 Fragments arranged by size: 3923 2029 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2216 2953 5773 Size: 107 122 71 631 605 544 737 2820 Cuts at: 5773 136 Size: 1525 Fragments arranged by size: 2820 1525 737 631 605 544 122 107 71 BglII A'GATC_T Cuts at: 3861 7157 3861 Size: 3296 3866 BlpI GC'TnA_GC Cuts at: 2528 3704 4579 2528 Size: 1176 875 5111 Fragments arranged by size: 5111 1176 875 Bme1580I G_kGCm'C Cuts at: 1790 2221 2228 2445 2942 3128 3331 3602 5223 Size: 431 7 217 497 186 203 271 1621 Cuts at: 5223 6379 1790 Size: 1156 2573 Fragments arranged by size: 2573 1621 1156 497 431 271 217 203 186 7 BmgBI CAC'GTC Cuts at: 1318 2590 3673 1318 Size: 1272 1083 4807 Fragments arranged by size: 4807 1272 1083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4893 5851 442 Size: 416 53 83 399 24 3476 958 1753 Fragments arranged by size: 3476 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7162 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2934 4087 2934 Size: 1153 6009 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2225 2306 2357 2467 2615 2784 2858 Size: 965 21 81 51 110 148 169 74 Cuts at: 2858 2904 2934 3081 5822 1239 Size: 46 30 147 2741 2579 Fragments arranged by size: 2741 2579 965 169 148 147 110 81 74 51 46 30 21 Bpu10I CC'TnA_GC Cuts at: 2889 3372 3378 2889 Size: 483 6 6673 Fragments arranged by size: 6673 483 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3899 5287 6152 6450 3899 Size: 1388 865 298 4611 Fragments arranged by size: 4611 1388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2671 2830 3277 3515 4154 5825 882 Size: 617 1172 159 447 238 639 1671 2219 Fragments arranged by size: 2219 1671 1172 639 617 447 238 159 BsaAI yAC'GTr Cuts at: 493 1204 3712 3778 4953 493 Size: 711 2508 66 1175 2702 Fragments arranged by size: 2702 2508 1175 711 66 BsaBI GATnn'nnATC Cuts at: 3011 3689 7156 3011 Size: 678 3467 3017 Fragments arranged by size: 3467 3017 678 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3441 3735 4969 5351 Size: 53 83 186 936 1908 294 1234 382 Cuts at: 5351 5986 275 Size: 635 1451 Fragments arranged by size: 1908 1451 1234 936 635 382 294 186 83 53 BsaWI w'CCGG_w Cuts at: 3403 3801 5595 5993 6267 6414 6825 3403 Size: 398 1794 398 274 147 411 3740 Fragments arranged by size: 3740 1794 411 398 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2673 2703 3079 3109 1178 Size: 30 165 30 1270 30 376 30 5231 Fragments arranged by size: 5231 1270 376 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2464 2492 3263 3266 4241 1186 Size: 1278 28 771 3 975 4107 Fragments arranged by size: 4107 1278 975 771 28 3 BseYI C'CCAG_C Cuts at: 2267 2319 2638 2698 2765 3108 3263 4188 6365 Size: 52 319 60 67 343 155 925 2177 Cuts at: 6365 6861 2267 Size: 496 2568 Fragments arranged by size: 2568 2177 925 496 343 319 155 67 60 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2180 2874 3411 3742 3869 3887 Size: 573 372 72 694 537 331 127 18 Cuts at: 3887 1163 Size: 4438 Fragments arranged by size: 4438 694 573 537 372 331 127 72 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3802 4612 5373 6401 1540 Size: 275 186 1801 810 761 1028 2301 Fragments arranged by size: 2301 1801 1028 810 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2534 3204 3602 3795 5223 5308 6379 2534 Size: 670 398 193 1428 85 1071 3317 Fragments arranged by size: 3317 1428 1071 670 398 193 85 BsmI GAATG_Cn' Cuts at: 2708 4673 4766 2708 Size: 1965 93 5104 Fragments arranged by size: 5104 1965 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4358 4358 Size: 7162 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2221 2228 2445 2534 2895 2942 3128 Size: 399 431 7 217 89 361 47 186 Cuts at: 3128 3171 3204 3331 3403 3602 3795 4221 4481 Size: 43 33 127 72 199 193 426 260 Cuts at: 4481 5223 5308 6379 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 431 426 399 361 260 217 199 193 186 127 89 85 72 47 43 33 7 BspEI T'CCGG_A Cuts at: 6825 6825 Size: 7162 BspHI T'CATG_A Cuts at: 3693 5051 6781 3693 Size: 1358 1730 4074 Fragments arranged by size: 4074 1730 1358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 2730 3891 4517 844 Size: 1228 658 1161 626 3489 Fragments arranged by size: 3489 1228 1161 658 626 BsrBI CCG'CTC Cuts at: 1394 1823 2188 5049 1394 Size: 429 365 2861 3507 Fragments arranged by size: 3507 2861 429 365 BsrDI GCAATG_nn' Cuts at: 65 1248 5657 5831 65 Size: 1183 4409 174 1396 Fragments arranged by size: 4409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5806 5993 1700 Size: 110 3996 187 2869 Fragments arranged by size: 3996 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7162 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7162 BssSI C'ACGA_G Cuts at: 2908 3280 3317 4304 5222 6234 2908 Size: 372 37 987 918 1012 3836 Fragments arranged by size: 3836 1012 987 918 372 37 BstAPI GCAn_nnn'nTGC Cuts at: 794 2064 2852 3883 794 Size: 1270 788 1031 4073 Fragments arranged by size: 4073 1270 1031 788 BstBI TT'CG_AA Cuts at: 5966 5966 Size: 7162 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7162 BstXI CCAn_nnnn'nTGG Cuts at: 2383 2684 2740 2987 3158 3323 2383 Size: 301 56 247 171 165 6222 Fragments arranged by size: 6222 301 247 171 165 56 BstYI r'GATC_y Cuts at: 1972 3006 3861 4340 4492 4859 5244 5261 6713 Size: 1034 855 479 152 367 385 17 1452 Cuts at: 6713 6799 6811 7157 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1034 855 479 385 367 346 152 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2475 2777 2475 Size: 302 6860 BtgI C'CryG_G Cuts at: 513 1065 2076 513 Size: 552 1011 5599 Fragments arranged by size: 5599 1011 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2362 487 Size: 683 56 1136 5287 Fragments arranged by size: 5287 1136 683 56 BtsI GCAGTG_nn' Cuts at: 2667 4678 5472 5492 2667 Size: 2011 794 20 4337 Fragments arranged by size: 4337 2011 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7162 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7127 DraI TTT'AAA Cuts at: 4466 4466 Size: 7162 DraIII CAC_nnn'GTG Cuts at: 2852 4293 2852 Size: 1441 5721 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6169 809 Size: 507 4853 1802 Fragments arranged by size: 4853 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2716 3294 3583 Size: 54 1425 50 275 18 886 578 289 Cuts at: 3583 3981 4609 5498 7127 8 Size: 398 628 889 1629 43 Fragments arranged by size: 1629 1425 889 886 628 578 398 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4609 1537 Size: 275 2797 4090 Fragments arranged by size: 4090 2797 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2351 2496 2565 3644 3800 4262 5092 1353 Size: 998 145 69 1079 156 462 830 3423 Fragments arranged by size: 3423 1079 998 830 462 156 145 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2432 2600 5749 6123 6269 387 Size: 1064 138 843 168 3149 374 146 1280 Fragments arranged by size: 3149 1280 1064 843 374 168 146 138 EcoICRI GAG'CTC Cuts at: 3202 3793 3202 Size: 591 6571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2225 2306 2332 2357 2467 2615 Size: 393 572 21 81 26 25 110 148 Cuts at: 2615 2784 2858 2904 2934 3081 3207 3435 4031 Size: 169 74 46 30 147 126 228 596 Cuts at: 4031 5225 5822 6609 6877 1239 Size: 1194 597 787 268 1524 Fragments arranged by size: 1524 1194 787 597 596 572 393 268 228 169 148 147 126 110 81 74 46 30 26 25 21 EcoNI CCTnn'n_nnAGG Cuts at: 2429 3341 2429 Size: 912 6250 EcoO109I rG'GnC_Cy Cuts at: 2191 2281 2341 2924 2945 3124 3327 3328 2191 Size: 90 60 583 21 179 203 1 6025 Fragments arranged by size: 6025 583 203 179 90 60 21 1 EcoRI G'AATT_C Cuts at: 4471 4849 4471 Size: 378 6784 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2083 4036 2083 Size: 1953 5209 FspI TGC'GCA Cuts at: 5668 5668 Size: 7162 HaeII r_GCGC'y Cuts at: 1678 3145 3444 3657 6309 1678 Size: 1467 299 213 2652 2531 Fragments arranged by size: 2652 2531 1467 299 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3019 3051 3066 3098 3937 Size: 32 135 32 1447 32 15 32 839 Cuts at: 3937 3969 5803 5835 5877 5909 1373 Size: 32 1834 32 42 32 2626 Fragments arranged by size: 2626 1834 1447 839 135 42 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 4506 4752 669 Size: 988 281 2568 246 3079 Fragments arranged by size: 3079 2568 988 281 246 HindIII A'AGCT_T Cuts at: 748 3289 4332 748 Size: 2541 1043 3578 Fragments arranged by size: 3578 2541 1043 HpaI GTT'AAC Cuts at: 4752 4752 Size: 7162 KasI G'GCGC_C Cuts at: 3440 3440 Size: 7162 KpnI G_GTAC'C Cuts at: 4487 4487 Size: 7162 MfeI C'AATT_G Cuts at: 4761 4761 Size: 7162 MluI A'CGCG_T Cuts at: 7038 7038 Size: 7162 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4475 6276 6460 1305 Size: 3170 1801 184 2007 Fragments arranged by size: 3170 2007 1801 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2718 3296 3585 3983 10 Size: 54 1425 343 886 578 289 398 3189 Fragments arranged by size: 3189 1425 886 578 398 343 289 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 2985 3644 4085 4112 4413 5120 Size: 648 1572 247 659 441 27 301 707 Cuts at: 5120 5479 5638 518 Size: 359 159 2042 Fragments arranged by size: 2042 1572 707 659 648 441 359 301 247 159 27 MspA1I CmG'CkG Cuts at: 1606 2606 3104 3211 3756 4578 5255 6403 6648 Size: 1000 498 107 545 822 677 1148 245 Cuts at: 6648 1606 Size: 2120 Fragments arranged by size: 2120 1148 1000 822 677 545 498 245 107 NarI GG'CG_CC Cuts at: 3441 3441 Size: 7162 NcoI C'CATG_G Cuts at: 513 1065 2076 513 Size: 552 1011 5599 Fragments arranged by size: 5599 1011 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7162 NotI GC'GGCC_GC Cuts at: 4609 4609 Size: 7162 NspI r_CATG'y Cuts at: 1132 1229 2098 2164 2525 4090 4117 4520 6065 Size: 97 869 66 361 1565 27 403 1545 Cuts at: 6065 1132 Size: 2229 Fragments arranged by size: 2229 1565 1545 869 403 361 97 66 27 PasI CC'CwG_GG Cuts at: 1348 2942 3158 3331 3356 1348 Size: 1594 216 173 25 5154 Fragments arranged by size: 5154 1594 216 173 25 PciI A'CATG_T Cuts at: 4086 6061 4086 Size: 1975 5187 PflMI CCAn_nnn'nTGG Cuts at: 1108 2662 2927 1108 Size: 1554 265 5343 Fragments arranged by size: 5343 1554 265 PfoI T'CCnGG_A Cuts at: 2283 2283 Size: 7162 PmeI GTTT'AAAC Cuts at: 4466 4466 Size: 7162 PmlI CAC'GTG Cuts at: 3712 3712 Size: 7162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2673 2705 5231 5263 6744 6776 2673 Size: 32 2526 32 1481 32 3059 Fragments arranged by size: 3059 2526 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 2281 Size: 7162 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7162 PsiI TTA'TAA Cuts at: 4732 4732 Size: 7162 PspOMI G'GGCC_C Cuts at: 2224 3124 3327 2224 Size: 900 203 6059 Fragments arranged by size: 6059 900 203 PstI C_TGCA'G Cuts at: 830 3216 3342 4277 4377 4514 830 Size: 2386 126 935 100 137 3478 Fragments arranged by size: 3478 2386 935 137 126 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7162 PvuII CAG'CTG Cuts at: 3104 3756 3104 Size: 652 6510 SacI G_AGCT'C Cuts at: 3204 3795 3204 Size: 591 6571 SalI G'TCGA_C Cuts at: 1936 4504 1936 Size: 2568 4594 SapI GCTCTTCn'nnn_ Cuts at: 3644 3800 4262 3644 Size: 156 462 6544 Fragments arranged by size: 6544 462 156 SbfI CC_TGCA'GG Cuts at: 4514 4514 Size: 7162 ScaI AGT'ACT Cuts at: 5410 7122 5410 Size: 1712 5450 SexAI A'CCwGG_T Cuts at: 2848 2848 Size: 7162 SfcI C'TryA_G Cuts at: 826 1045 3212 3338 4183 4273 4373 4510 5645 Size: 219 2167 126 845 90 100 137 1135 Cuts at: 5645 6326 6517 826 Size: 681 191 1471 Fragments arranged by size: 2167 1471 1135 845 681 219 191 137 126 100 90 SfoI GGC'GCC Cuts at: 3442 3442 Size: 7162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7162 SmaI CCC'GGG Cuts at: 1872 2216 2229 3232 4489 1872 Size: 344 13 1003 1257 4545 Fragments arranged by size: 4545 1257 1003 344 13 SmlI C'TyrA_G Cuts at: 820 1017 1943 3914 3999 5266 6167 6429 820 Size: 197 926 1971 85 1267 901 262 1553 Fragments arranged by size: 1971 1553 1267 926 901 262 197 85 SnaBI TAC'GTA Cuts at: 493 4953 493 Size: 4460 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7162 SphI G_CATG'C Cuts at: 1132 4520 1132 Size: 3388 3774 SrfI GCCC'GGGC Cuts at: 2229 2229 Size: 7162 SspI AAT'ATT Cuts at: 5 52 5086 5 Size: 47 5034 2081 Fragments arranged by size: 5034 2081 47 StuI AGG'CCT Cuts at: 1517 2210 2881 3618 1517 Size: 693 671 737 5061 Fragments arranged by size: 5061 737 693 671 StyI C'CwwG_G Cuts at: 513 1065 2019 2076 2748 3002 3070 3657 4233 Size: 552 954 57 672 254 68 587 576 Cuts at: 4233 4601 513 Size: 368 3074 Fragments arranged by size: 3074 954 672 587 576 552 368 254 68 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2259 2276 2779 5189 5206 5359 5544 Size: 1255 679 17 503 2410 17 153 185 Cuts at: 5544 5989 325 Size: 445 1498 Fragments arranged by size: 2410 1498 1255 679 503 445 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5408 7120 96 Size: 274 80 33 51 1443 3431 1712 138 Fragments arranged by size: 3431 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3079 3111 7122 7154 3079 Size: 32 4011 32 3087 Fragments arranged by size: 4011 3087 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7162 XbaI T'CTAG_A Cuts at: 4498 4498 Size: 7162 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2026 2383 2711 2026 Size: 357 328 6477 Fragments arranged by size: 6477 357 328 XhoI C'TCGA_G Cuts at: 1943 3999 1943 Size: 2056 5106 XmaI C'CCGG_G Cuts at: 1870 2214 2227 3230 4487 1870 Size: 344 13 1003 1257 4545 Fragments arranged by size: 4545 1257 1003 344 13 XmnI GAAnn'nnTTC Cuts at: 4409 5291 4409 Size: 882 6280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4970 276 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SwaI