(Linear) (Six-base) MAPSORT of: KIEE3538.seq Check: 1917 from: 1 to: 2457 KIEE3538.orf With 182 enzymes: SgfI * July 3, 2012 18:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 725 2457 Size: 725 1732 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 327 1202 1430 2026 2457 Size: 327 875 228 596 431 Fragments arranged by size: 875 596 431 327 228 AflIII A'CryG_T Cuts at: 0 2081 2457 Size: 2081 376 AleI CACnn'nnGTG Cuts at: 0 733 980 2457 Size: 733 247 1477 Fragments arranged by size: 1477 733 247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 323 563 1400 2457 Size: 323 240 837 1057 Fragments arranged by size: 1057 837 323 240 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 668 700 2457 Size: 668 32 1757 Fragments arranged by size: 1757 668 32 AlwNI CAG_nnn'CTG Cuts at: 0 117 922 1099 2292 2457 Size: 117 805 177 1193 165 Fragments arranged by size: 1193 805 177 165 117 ApaI G_GGCC'C Cuts at: 0 223 1123 1326 2457 Size: 223 900 203 1131 Fragments arranged by size: 1131 900 223 203 ApaLI G'TGCA_C Cuts at: 0 1593 2457 Size: 1593 864 ApoI r'AATT_y Cuts at: 0 2163 2440 2457 Size: 2163 277 17 Fragments arranged by size: 2163 277 17 AvaI C'yCGr_G Cuts at: 0 209 222 271 825 1225 1994 2457 Size: 209 13 49 554 400 769 463 Fragments arranged by size: 769 554 463 400 209 49 13 BanI G'GyrC_C Cuts at: 0 213 322 1435 2457 Size: 213 109 1113 1022 Fragments arranged by size: 1113 1022 213 109 BanII G_rGCy'C Cuts at: 0 223 890 1123 1166 1199 1326 1398 1790 Size: 223 667 233 43 33 127 72 392 Cuts at: 1790 2216 2457 Size: 426 241 Fragments arranged by size: 667 426 392 241 233 223 127 72 43 33 BbeI G_GCGC'C Cuts at: 0 1439 2457 Size: 1439 1018 BciVI GTATCCnnnnn_n' Cuts at: 0 1020 2457 Size: 1020 1437 BglI GCCn_nnn'nGGC Cuts at: 0 211 948 2457 Size: 211 737 1509 Fragments arranged by size: 1509 737 211 BglII A'GATC_T Cuts at: 0 1856 2457 Size: 1856 601 BlpI GC'TnA_GC Cuts at: 0 523 1699 2457 Size: 523 1176 758 Fragments arranged by size: 1176 758 523 Bme1580I G_kGCm'C Cuts at: 0 216 223 440 937 1123 1326 1597 2457 Size: 216 7 217 497 186 203 271 860 Fragments arranged by size: 860 497 271 217 216 203 186 7 BmgBI CAC'GTC Cuts at: 0 585 1668 2457 Size: 585 1083 789 Fragments arranged by size: 1083 789 585 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 929 2082 2457 Size: 929 1153 375 Fragments arranged by size: 1153 929 375 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 220 301 352 462 610 779 853 Size: 199 21 81 51 110 148 169 74 Cuts at: 853 899 929 1076 2457 Size: 46 30 147 1381 Fragments arranged by size: 1381 199 169 148 147 110 81 74 51 46 30 21 Bpu10I CC'TnA_GC Cuts at: 0 884 1367 1373 2457 Size: 884 483 6 1084 Fragments arranged by size: 1084 884 483 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1894 2457 Size: 1894 563 BsaI GGTCTCn'nnnn_ Cuts at: 0 666 825 1272 1510 2149 2457 Size: 666 159 447 238 639 308 Fragments arranged by size: 666 639 447 308 238 159 BsaAI yAC'GTr Cuts at: 0 1707 1773 2457 Size: 1707 66 684 Fragments arranged by size: 1707 684 66 BsaBI GATnn'nnATC Cuts at: 0 1006 1684 2457 Size: 1006 678 773 Fragments arranged by size: 1006 773 678 BsaHI Gr'CG_yC Cuts at: 0 1436 1730 2457 Size: 1436 294 727 Fragments arranged by size: 1436 727 294 BsaWI w'CCGG_w Cuts at: 0 1398 1796 2457 Size: 1398 398 661 Fragments arranged by size: 1398 661 398 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 668 698 1074 1104 2457 Size: 668 30 376 30 1353 Fragments arranged by size: 1353 668 376 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 459 487 1258 1261 2236 2457 Size: 459 28 771 3 975 221 Fragments arranged by size: 975 771 459 221 28 3 BseYI C'CCAG_C Cuts at: 0 262 314 633 693 760 1103 1258 2183 Size: 262 52 319 60 67 343 155 925 Cuts at: 2183 2457 Size: 274 Fragments arranged by size: 925 343 319 274 262 155 67 60 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 175 869 1406 1737 1864 1882 2457 Size: 103 72 694 537 331 127 18 575 Fragments arranged by size: 694 575 537 331 127 103 72 18 BsiEI CG_ry'CG Cuts at: 0 1797 2457 Size: 1797 660 BsiHKAI G_wGCw'C Cuts at: 0 529 1199 1597 1790 2457 Size: 529 670 398 193 667 Fragments arranged by size: 670 667 529 398 193 BsmI GAATG_Cn' Cuts at: 0 703 2457 Size: 703 1754 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2353 2457 Size: 2353 104 Bsp1286I G_dGCh'C Cuts at: 0 216 223 440 529 890 937 1123 1166 Size: 216 7 217 89 361 47 186 43 Cuts at: 1166 1199 1326 1398 1597 1790 2216 2457 Size: 33 127 72 199 193 426 241 Fragments arranged by size: 426 361 241 217 216 199 193 186 127 89 72 47 43 33 7 BspHI T'CATG_A Cuts at: 0 1688 2457 Size: 1688 769 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 67 725 1886 2457 Size: 67 658 1161 571 Fragments arranged by size: 1161 658 571 67 BsrBI CCG'CTC Cuts at: 0 183 2457 Size: 183 2274 BssSI C'ACGA_G Cuts at: 0 903 1275 1312 2299 2457 Size: 903 372 37 987 158 Fragments arranged by size: 987 903 372 158 37 BstAPI GCAn_nnn'nTGC Cuts at: 0 59 847 1878 2457 Size: 59 788 1031 579 Fragments arranged by size: 1031 788 579 59 BstXI CCAn_nnnn'nTGG Cuts at: 0 378 679 735 982 1153 1318 2457 Size: 378 301 56 247 171 165 1139 Fragments arranged by size: 1139 378 301 247 171 165 56 BstYI r'GATC_y Cuts at: 0 1001 1856 2335 2457 Size: 1001 855 479 122 Fragments arranged by size: 1001 855 479 122 Bsu36I CC'TnA_GG Cuts at: 0 470 772 2457 Size: 470 302 1685 Fragments arranged by size: 1685 470 302 BtgI C'CryG_G Cuts at: 0 71 2457 Size: 71 2386 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 357 2457 Size: 357 2100 BtsI GCAGTG_nn' Cuts at: 0 662 2457 Size: 662 1795 DraIII CAC_nnn'GTG Cuts at: 0 847 2288 2457 Size: 847 1441 169 Fragments arranged by size: 1441 847 169 EaeI y'GGCC_r Cuts at: 0 711 1289 1578 1976 2457 Size: 711 578 289 398 481 Fragments arranged by size: 711 578 481 398 289 EarI CTCTTCn'nnn_ Cuts at: 0 346 491 560 1639 1795 2257 2457 Size: 346 145 69 1079 156 462 200 Fragments arranged by size: 1079 462 346 200 156 145 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 427 595 2457 Size: 427 168 1862 Fragments arranged by size: 1862 427 168 EcoICRI GAG'CTC Cuts at: 0 1197 1788 2457 Size: 1197 591 669 Fragments arranged by size: 1197 669 591 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 220 301 327 352 462 610 779 Size: 199 21 81 26 25 110 148 169 Cuts at: 779 853 899 929 1076 1202 1430 2026 2457 Size: 74 46 30 147 126 228 596 431 Fragments arranged by size: 596 431 228 199 169 148 147 126 110 81 74 46 30 26 25 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 424 1336 2457 Size: 424 912 1121 Fragments arranged by size: 1121 912 424 EcoO109I rG'GnC_Cy Cuts at: 0 186 276 336 919 940 1119 1322 1323 Size: 186 90 60 583 21 179 203 1 Cuts at: 1323 2457 Size: 1134 Fragments arranged by size: 1134 583 203 186 179 90 60 21 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 78 2031 2457 Size: 78 1953 426 Fragments arranged by size: 1953 426 78 HaeII r_GCGC'y Cuts at: 0 1140 1439 1652 2457 Size: 1140 299 213 805 Fragments arranged by size: 1140 805 299 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1014 1046 1061 1093 1932 1964 2457 Size: 1014 32 15 32 839 32 493 Fragments arranged by size: 1014 839 493 32 32 32 15 HindIII A'AGCT_T Cuts at: 0 1284 2327 2457 Size: 1284 1043 130 Fragments arranged by size: 1284 1043 130 KasI G'GCGC_C Cuts at: 0 1435 2457 Size: 1435 1022 MscI TGG'CCA Cuts at: 0 713 1291 1580 1978 2457 Size: 713 578 289 398 479 Fragments arranged by size: 713 578 479 398 289 MslI CAynn'nnrTG Cuts at: 0 733 980 1639 2080 2107 2408 2457 Size: 733 247 659 441 27 301 49 Fragments arranged by size: 733 659 441 301 247 49 27 MspA1I CmG'CkG Cuts at: 0 601 1099 1206 1751 2457 Size: 601 498 107 545 706 Fragments arranged by size: 706 601 545 498 107 NarI GG'CG_CC Cuts at: 0 1436 2457 Size: 1436 1021 NcoI C'CATG_G Cuts at: 0 71 2457 Size: 71 2386 NspI r_CATG'y Cuts at: 0 93 159 520 2085 2112 2457 Size: 93 66 361 1565 27 345 Fragments arranged by size: 1565 361 345 93 66 27 PasI CC'CwG_GG Cuts at: 0 937 1153 1326 1351 2457 Size: 937 216 173 25 1106 Fragments arranged by size: 1106 937 216 173 25 PciI A'CATG_T Cuts at: 0 2081 2457 Size: 2081 376 PflMI CCAn_nnn'nTGG Cuts at: 0 657 922 2457 Size: 657 265 1535 Fragments arranged by size: 1535 657 265 PfoI T'CCnGG_A Cuts at: 0 278 2457 Size: 278 2179 PmlI CAC'GTG Cuts at: 0 1707 2457 Size: 1707 750 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 668 700 2457 Size: 668 32 1757 Fragments arranged by size: 1757 668 32 PpuMI rG'GwC_Cy Cuts at: 0 276 2457 Size: 276 2181 PspOMI G'GGCC_C Cuts at: 0 219 1119 1322 2457 Size: 219 900 203 1135 Fragments arranged by size: 1135 900 219 203 PstI C_TGCA'G Cuts at: 0 1211 1337 2272 2372 2457 Size: 1211 126 935 100 85 Fragments arranged by size: 1211 935 126 100 85 PvuII CAG'CTG Cuts at: 0 1099 1751 2457 Size: 1099 652 706 Fragments arranged by size: 1099 706 652 SacI G_AGCT'C Cuts at: 0 1199 1790 2457 Size: 1199 591 667 Fragments arranged by size: 1199 667 591 SapI GCTCTTCn'nnn_ Cuts at: 0 1639 1795 2257 2457 Size: 1639 156 462 200 Fragments arranged by size: 1639 462 200 156 SexAI A'CCwGG_T Cuts at: 0 843 2457 Size: 843 1614 SfcI C'TryA_G Cuts at: 0 1207 1333 2178 2268 2368 2457 Size: 1207 126 845 90 100 89 Fragments arranged by size: 1207 845 126 100 90 89 SfoI GGC'GCC Cuts at: 0 1437 2457 Size: 1437 1020 SmaI CCC'GGG Cuts at: 0 211 224 1227 2457 Size: 211 13 1003 1230 Fragments arranged by size: 1230 1003 211 13 SmlI C'TyrA_G Cuts at: 0 1909 1994 2457 Size: 1909 85 463 Fragments arranged by size: 1909 463 85 SrfI GCCC'GGGC Cuts at: 0 224 2457 Size: 224 2233 StuI AGG'CCT Cuts at: 0 205 876 1613 2457 Size: 205 671 737 844 Fragments arranged by size: 844 737 671 205 StyI C'CwwG_G Cuts at: 0 14 71 743 997 1065 1652 2228 2457 Size: 14 57 672 254 68 587 576 229 Fragments arranged by size: 672 587 576 254 229 68 57 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 254 271 774 2457 Size: 254 17 503 1683 Fragments arranged by size: 1683 503 254 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1074 1106 2457 Size: 1074 32 1351 Fragments arranged by size: 1351 1074 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 21 378 706 2457 Size: 21 357 328 1751 Fragments arranged by size: 1751 357 328 21 XhoI C'TCGA_G Cuts at: 0 1994 2457 Size: 1994 463 XmaI C'CCGG_G Cuts at: 0 209 222 1225 2457 Size: 209 13 1003 1232 Fragments arranged by size: 1232 1003 209 13 XmnI GAAnn'nnTTC Cuts at: 0 2404 2457 Size: 2404 53 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BciVI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI MscI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI SexAI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI BmrI BmtI BsiWI BspEI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI ZraI