(Circular) (Six-base) MAPSORT of: pFN21AE1798.seq Check: 3213 from: 1 to: 7111 pFN21AE1798 7111 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4921 278 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4454 1937 Size: 2517 4594 Acc65I G'GTAC_C Cuts at: 4432 4432 Size: 7111 AclI AA'CG_TT Cuts at: 1563 5238 5611 1563 Size: 3675 373 3063 Fragments arranged by size: 3675 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2180 3170 3535 3761 4184 5174 6558 6826 Size: 548 990 365 226 423 990 1384 268 Cuts at: 6826 1632 Size: 1917 Fragments arranged by size: 1917 1384 990 990 548 423 365 268 226 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6914 AflIII A'CryG_T Cuts at: 1203 3483 3893 6010 6987 1203 Size: 2280 410 2117 977 1327 Fragments arranged by size: 2280 2117 1327 977 410 AgeI A'CCGG_T Cuts at: 5942 5942 Size: 7111 AhdI GACnn_n'nnGTC Cuts at: 5840 5840 Size: 7111 AleI CACnn'nnGTG Cuts at: 1166 3552 1166 Size: 2386 4725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7111 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2298 2330 2298 Size: 32 7079 AlwNI CAG_nnn'CTG Cuts at: 3739 4409 4854 6426 3739 Size: 670 445 1572 4424 Fragments arranged by size: 4424 1572 670 445 ApaLI G'TGCA_C Cuts at: 3626 5168 6324 3626 Size: 1542 1156 4413 Fragments arranged by size: 4413 1542 1156 ApoI r'AATT_y Cuts at: 1493 2146 2788 3649 4420 4646 4798 6907 1493 Size: 653 642 861 771 226 152 2109 1697 Fragments arranged by size: 2109 1697 861 771 653 642 226 152 AseI AT'TA_AT Cuts at: 160 1032 5665 160 Size: 872 4633 1606 Fragments arranged by size: 4633 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 AvaI C'yCGr_G Cuts at: 1870 1943 4436 1870 Size: 73 2493 4545 Fragments arranged by size: 4545 2493 73 AvrII C'CTAG_G Cuts at: 2167 2167 Size: 7111 BamHI G'GATC_C Cuts at: 3070 4441 4808 3070 Size: 1371 367 5373 Fragments arranged by size: 5373 1371 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2665 4432 5887 618 Size: 325 217 627 246 632 1767 1455 1842 Fragments arranged by size: 1842 1767 1455 632 627 325 246 217 BanII G_rGCy'C Cuts at: 1391 2157 3783 4430 1391 Size: 766 1626 647 4072 Fragments arranged by size: 4072 1626 766 647 BbeI G_GCGC'C Cuts at: 2037 2037 Size: 7111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3749 928 Size: 971 1850 4290 Fragments arranged by size: 4290 1850 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5302 5336 1903 Size: 34 3365 34 3678 Fragments arranged by size: 3678 3365 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3982 5003 6213 3982 Size: 1021 1210 4880 Fragments arranged by size: 4880 1210 1021 BclI T'GATC_A Cuts at: 1548 3472 1548 Size: 1924 5187 BfrBI ATG'CAT Cuts at: 3610 3610 Size: 7111 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4223 5722 136 Size: 107 122 71 631 605 2551 1499 1525 Fragments arranged by size: 2551 1525 1499 631 605 122 107 71 BglII A'GATC_T Cuts at: 7106 7106 Size: 7111 BlpI GC'TnA_GC Cuts at: 4528 4528 Size: 7111 Bme1580I G_kGCm'C Cuts at: 1790 3630 5172 6328 1790 Size: 1840 1542 1156 2573 Fragments arranged by size: 2573 1840 1542 1156 BmgBI CAC'GTC Cuts at: 1318 3894 1318 Size: 2576 4535 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4842 5800 442 Size: 416 53 83 399 24 3425 958 1753 Fragments arranged by size: 3425 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7111 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2093 2093 Size: 7111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2196 2775 3234 5771 1239 Size: 957 579 459 2537 2579 Fragments arranged by size: 2579 2537 957 579 459 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 4066 5236 6101 6399 2564 Size: 1502 1170 865 298 3276 Fragments arranged by size: 3276 1502 1170 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 5774 882 Size: 617 2874 1401 2219 Fragments arranged by size: 2874 2219 1401 617 BsaAI yAC'GTr Cuts at: 493 1204 2924 4902 493 Size: 711 1720 1978 2702 Fragments arranged by size: 2702 1978 1720 711 BsaBI GATnn'nnATC Cuts at: 7105 7105 Size: 7111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 3831 4918 5300 Size: 53 83 186 936 501 1797 1087 382 Cuts at: 5300 5935 275 Size: 635 1451 Fragments arranged by size: 1797 1451 1087 936 635 501 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3724 4105 4124 5544 5942 6216 6363 6774 3724 Size: 381 19 1420 398 274 147 411 4061 Fragments arranged by size: 4061 1420 411 398 381 274 147 19 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2472 2502 2713 2743 3067 Size: 30 165 30 1069 30 211 30 324 Cuts at: 3067 3097 4119 4149 1178 Size: 30 1022 30 4140 Fragments arranged by size: 4140 1069 1022 324 211 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2027 2069 2082 3528 1186 Size: 838 3 42 13 1446 4769 Fragments arranged by size: 4769 1446 838 42 13 3 BseYI C'CCAG_C Cuts at: 4349 6314 6810 4349 Size: 1965 496 4650 Fragments arranged by size: 4650 1965 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2486 2985 1163 Size: 573 750 499 5289 Fragments arranged by size: 5289 750 573 499 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4561 5322 6350 1540 Size: 275 186 2560 761 1028 2301 Fragments arranged by size: 2560 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 3630 3783 5172 5257 6328 2157 Size: 1473 153 1389 85 1071 2940 Fragments arranged by size: 2940 1473 1389 1071 153 85 BsmI GAATG_Cn' Cuts at: 4622 4715 4622 Size: 93 7018 BsmBI CGTCTCn'nnnn_ Cuts at: 3551 4259 3551 Size: 708 6403 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 3630 3783 4430 5172 5257 6328 Size: 399 367 1473 153 647 742 85 1071 Cuts at: 6328 1391 Size: 2174 Fragments arranged by size: 2174 1473 1071 742 647 399 367 153 85 BspEI T'CCGG_A Cuts at: 4105 4124 6774 4105 Size: 19 2650 4442 Fragments arranged by size: 4442 2650 19 BspHI T'CATG_A Cuts at: 3469 3576 3877 3915 5000 6730 3469 Size: 107 301 38 1085 1730 3850 Fragments arranged by size: 3850 1730 1085 301 107 38 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4466 844 Size: 3622 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4998 1394 Size: 429 3175 3507 Fragments arranged by size: 3507 3175 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3246 5606 5780 65 Size: 1183 1998 2360 174 1396 Fragments arranged by size: 2360 1998 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5755 5942 1700 Size: 110 3945 187 2869 Fragments arranged by size: 3945 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3840 96 Size: 3744 3367 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7111 BssSI C'ACGA_G Cuts at: 2093 5171 6183 2093 Size: 3078 1012 3021 Fragments arranged by size: 3078 3021 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2098 2464 794 Size: 1304 366 5441 Fragments arranged by size: 5441 1304 366 BstBI TT'CG_AA Cuts at: 5915 5915 Size: 7111 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7111 BstXI CCAn_nnnn'nTGG Cuts at: 2225 3907 2225 Size: 1682 5429 BstYI r'GATC_y Cuts at: 1972 2893 3070 3634 4015 4118 4441 4808 5193 Size: 921 177 564 381 103 323 367 385 Cuts at: 5193 5210 6662 6748 6760 7106 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 921 564 385 381 367 346 323 177 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 513 Size: 552 939 33 5587 Fragments arranged by size: 5587 939 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6372 Fragments arranged by size: 6372 683 56 BtsI GCAGTG_nn' Cuts at: 4340 4627 5421 5441 4340 Size: 287 794 20 6010 Fragments arranged by size: 6010 794 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7076 DraI TTT'AAA Cuts at: 4415 4415 Size: 7111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6118 809 Size: 507 4802 1802 Fragments arranged by size: 4802 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 2569 4558 Size: 54 1425 50 275 18 385 354 1989 Cuts at: 4558 5447 7076 8 Size: 889 1629 43 Fragments arranged by size: 1989 1629 1425 889 385 354 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4558 1537 Size: 275 2746 4090 Fragments arranged by size: 4090 2746 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2897 2957 3048 3189 4391 5041 1353 Size: 1544 60 91 141 1202 650 3423 Fragments arranged by size: 3423 1544 1202 650 141 91 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5698 6072 6218 387 Size: 1064 138 4109 374 146 1280 Fragments arranged by size: 4109 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2155 3781 2155 Size: 1626 5485 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2196 2775 3170 3234 3535 3761 Size: 393 548 16 579 395 64 301 226 Cuts at: 3761 4184 5174 5771 6558 6826 1239 Size: 423 990 597 787 268 1524 Fragments arranged by size: 1524 990 787 597 579 548 423 395 393 301 268 226 64 16 EcoO109I rG'GnC_Cy Cuts at: 2164 4216 2164 Size: 2052 5059 EcoRI G'AATT_C Cuts at: 4420 4798 4420 Size: 378 6733 EcoRV GAT'ATC Cuts at: 2805 2805 Size: 7111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3565 3565 Size: 7111 FspI TGC'GCA Cuts at: 5617 5617 Size: 7111 HaeII r_GCGC'y Cuts at: 1678 2037 6258 1678 Size: 359 4221 2531 Fragments arranged by size: 4221 2531 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2512 2544 2878 2910 5752 Size: 32 135 32 940 32 334 32 2842 Cuts at: 5752 5784 5826 5858 1373 Size: 32 42 32 2626 Fragments arranged by size: 2842 2626 940 334 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2713 4455 4701 669 Size: 988 281 775 1742 246 3079 Fragments arranged by size: 3079 1742 988 775 281 246 HindIII A'AGCT_T Cuts at: 748 2540 3354 3584 748 Size: 1792 814 230 4275 Fragments arranged by size: 4275 1792 814 230 HpaI GTT'AAC Cuts at: 4701 4701 Size: 7111 KasI G'GCGC_C Cuts at: 2033 2033 Size: 7111 KpnI G_GTAC'C Cuts at: 4436 4436 Size: 7111 MfeI C'AATT_G Cuts at: 4710 4710 Size: 7111 MluI A'CGCG_T Cuts at: 6987 6987 Size: 7111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2458 3598 6225 6409 1305 Size: 1153 1140 2627 184 2007 Fragments arranged by size: 2627 2007 1153 1140 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2217 2571 10 Size: 54 1425 343 385 354 4550 Fragments arranged by size: 4550 1425 385 354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3065 3552 3609 4143 5069 5428 5587 Size: 648 1899 487 57 534 926 359 159 Cuts at: 5587 518 Size: 2042 Fragments arranged by size: 2042 1899 926 648 534 487 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2028 2039 3170 3596 4081 4527 5204 6352 Size: 422 11 1131 426 485 446 677 1148 Cuts at: 6352 6597 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1131 677 485 446 426 422 245 11 NarI GG'CG_CC Cuts at: 2034 2034 Size: 7111 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5620 Fragments arranged by size: 5620 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7111 NotI GC'GGCC_GC Cuts at: 4558 4558 Size: 7111 NsiI A_TGCA'T Cuts at: 3612 3612 Size: 7111 NspI r_CATG'y Cuts at: 1132 1229 2126 3610 4469 6014 1132 Size: 97 897 1484 859 1545 2229 Fragments arranged by size: 2229 1545 1484 897 859 97 PasI CC'CwG_GG Cuts at: 1348 3298 1348 Size: 1950 5161 PciI A'CATG_T Cuts at: 6010 6010 Size: 7111 PflMI CCAn_nnn'nTGG Cuts at: 1108 2515 1108 Size: 1407 5704 PfoI T'CCnGG_A Cuts at: 2316 3211 2316 Size: 895 6216 PmeI GTTT'AAAC Cuts at: 4415 4415 Size: 7111 PmlI CAC'GTG Cuts at: 2924 2924 Size: 7111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5180 5212 6693 6725 5180 Size: 32 1481 32 5566 Fragments arranged by size: 5566 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2164 Size: 7111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7111 PsiI TTA'TAA Cuts at: 4681 4681 Size: 7111 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2184 2216 2184 Size: 32 7079 PstI C_TGCA'G Cuts at: 830 4347 4463 830 Size: 3517 116 3478 Fragments arranged by size: 3517 3478 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7111 PvuII CAG'CTG Cuts at: 3170 3596 4081 3170 Size: 426 485 6200 Fragments arranged by size: 6200 485 426 SacI G_AGCT'C Cuts at: 2157 3783 2157 Size: 1626 5485 SacII CC_GC'GG Cuts at: 2040 2040 Size: 7111 SalI G'TCGA_C Cuts at: 1936 4453 1936 Size: 2517 4594 SbfI CC_TGCA'GG Cuts at: 4463 4463 Size: 7111 ScaI AGT'ACT Cuts at: 5359 7071 5359 Size: 1712 5399 SfcI C'TryA_G Cuts at: 826 1045 4343 4459 5594 6275 6466 826 Size: 219 3298 116 1135 681 191 1471 Fragments arranged by size: 3298 1471 1135 681 219 191 116 SfoI GGC'GCC Cuts at: 2035 2035 Size: 7111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7111 SmaI CCC'GGG Cuts at: 1872 4438 1872 Size: 2566 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2543 4045 5215 6116 6378 820 Size: 197 926 600 1502 1170 901 262 1553 Fragments arranged by size: 1553 1502 1170 926 901 600 262 197 SnaBI TAC'GTA Cuts at: 493 4902 493 Size: 4409 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7111 SphI G_CATG'C Cuts at: 1132 4469 1132 Size: 3337 3774 SspI AAT'ATT Cuts at: 5 52 2551 2817 3445 5035 5 Size: 47 2499 266 628 1590 2081 Fragments arranged by size: 2499 2081 1590 628 266 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7111 StyI C'CwwG_G Cuts at: 513 1065 2004 2167 3997 4550 513 Size: 552 939 163 1830 553 3074 Fragments arranged by size: 3074 1830 939 553 552 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 3897 4345 5138 5155 5308 5493 Size: 1255 1137 1180 448 793 17 153 185 Cuts at: 5493 5938 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1180 1137 793 448 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3840 5357 7069 Size: 274 80 33 51 1443 1863 1517 1712 Cuts at: 7069 96 Size: 138 Fragments arranged by size: 1863 1712 1517 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2472 2504 2713 2745 3067 3099 4000 4032 4119 Size: 32 209 32 322 32 901 32 87 Cuts at: 4119 4151 7071 7103 2472 Size: 32 2920 32 2480 Fragments arranged by size: 2920 2480 901 322 209 87 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7111 XbaI T'CTAG_A Cuts at: 4447 4447 Size: 7111 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2905 2905 Size: 7111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7111 XmaI C'CCGG_G Cuts at: 1870 4436 1870 Size: 2566 4545 XmnI GAAnn'nnTTC Cuts at: 3465 3548 5240 3465 Size: 83 1692 5336 Fragments arranged by size: 5336 1692 83 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4919 276 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI RsrII SanDI SapI SexAI SfiI SrfI SwaI