Restriction map for
pFN21AE1798
(Linear) (Six-base) MAPSORT of: KIEE1798.seq Check: 8438 from: 1 to: 2406
KIEE1798.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1165 1530 1756 2179 2406
Size: 175 990 365 226 423 227
Fragments arranged by size:
990 423 365 227 226 175
AflIII A'CryG_T
Cuts at: 0 1478 1888 2406
Size: 1478 410 518
Fragments arranged by size:
1478 518 410
AleI CACnn'nnGTG
Cuts at: 0 1547 2406
Size: 1547 859
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 293 325 2406
Size: 293 32 2081
Fragments arranged by size:
2081 293 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1734 2406
Size: 1734 672
ApaLI G'TGCA_C
Cuts at: 0 1621 2406
Size: 1621 785
ApoI r'AATT_y
Cuts at: 0 141 783 1644 2406
Size: 141 642 861 762
Fragments arranged by size:
861 762 642 141
AvrII C'CTAG_G
Cuts at: 0 162 2406
Size: 162 2244
BamHI G'GATC_C
Cuts at: 0 1065 2406
Size: 1065 1341
BanI G'GyrC_C
Cuts at: 0 28 660 2406
Size: 28 632 1746
Fragments arranged by size:
1746 632 28
BanII G_rGCy'C
Cuts at: 0 152 1778 2406
Size: 152 1626 628
Fragments arranged by size:
1626 628 152
BbeI G_GCGC'C
Cuts at: 0 32 2406
Size: 32 2374
BbsI GAAGACnn'nnnn_
Cuts at: 0 1744 2406
Size: 1744 662
BciVI GTATCCnnnnn_n'
Cuts at: 0 1977 2406
Size: 1977 429
BclI T'GATC_A
Cuts at: 0 1467 2406
Size: 1467 939
BfrBI ATG'CAT
Cuts at: 0 1605 2406
Size: 1605 801
BglI GCCn_nnn'nGGC
Cuts at: 0 2218 2406
Size: 2218 188
Bme1580I G_kGCm'C
Cuts at: 0 1625 2406
Size: 1625 781
BmgBI CAC'GTC
Cuts at: 0 1889 2406
Size: 1889 517
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 2406
Size: 88 2318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 770 1229 2406
Size: 191 579 459 1177
Fragments arranged by size:
1177 579 459 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 2061 2406
Size: 559 1502 345
Fragments arranged by size:
1502 559 345
BsaI GGTCTCn'nnnn_
Cuts at: 0 2368 2406
Size: 2368 38
BsaAI yAC'GTr
Cuts at: 0 919 2406
Size: 919 1487
BsaHI Gr'CG_yC
Cuts at: 0 29 1826 2406
Size: 29 1797 580
Fragments arranged by size:
1797 580 29
BsaWI w'CCGG_w
Cuts at: 0 1719 2100 2119 2406
Size: 1719 381 19 287
Fragments arranged by size:
1719 381 287 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 467 497 708 738 1062 1092 2114 2144
Size: 467 30 211 30 324 30 1022 30
Cuts at: 2144 2406
Size: 262
Fragments arranged by size:
1022 467 324 262 211 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 22 64 77 1523 2406
Size: 19 3 42 13 1446 883
Fragments arranged by size:
1446 883 42 19 13 3
BseYI C'CCAG_C
Cuts at: 0 2344 2406
Size: 2344 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 980 2406
Size: 481 499 1426
Fragments arranged by size:
1426 499 481
BsiHKAI G_wGCw'C
Cuts at: 0 152 1625 1778 2406
Size: 152 1473 153 628
Fragments arranged by size:
1473 628 153 152
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1546 2254 2406
Size: 1546 708 152
Fragments arranged by size:
1546 708 152
Bsp1286I G_dGCh'C
Cuts at: 0 152 1625 1778 2406
Size: 152 1473 153 628
Fragments arranged by size:
1473 628 153 152
BspEI T'CCGG_A
Cuts at: 0 2100 2119 2406
Size: 2100 19 287
Fragments arranged by size:
2100 287 19
BspHI T'CATG_A
Cuts at: 0 1464 1571 1872 1910 2406
Size: 1464 107 301 38 496
Fragments arranged by size:
1464 496 301 107 38
BsrDI GCAATG_nn'
Cuts at: 0 1241 2406
Size: 1241 1165
BsrGI T'GTAC_A
Cuts at: 0 1835 2406
Size: 1835 571
BssSI C'ACGA_G
Cuts at: 0 88 2406
Size: 88 2318
BstAPI GCAn_nnn'nTGC
Cuts at: 0 93 459 2406
Size: 93 366 1947
Fragments arranged by size:
1947 366 93
BstXI CCAn_nnnn'nTGG
Cuts at: 0 220 1902 2406
Size: 220 1682 504
Fragments arranged by size:
1682 504 220
BstYI r'GATC_y
Cuts at: 0 888 1065 1629 2010 2113 2406
Size: 888 177 564 381 103 293
Fragments arranged by size:
888 564 381 293 177 103
BtgI C'CryG_G
Cuts at: 0 32 2406
Size: 32 2374
BtsI GCAGTG_nn'
Cuts at: 0 2335 2406
Size: 2335 71
EaeI y'GGCC_r
Cuts at: 0 210 564 2406
Size: 210 354 1842
Fragments arranged by size:
1842 354 210
EarI CTCTTCn'nnn_
Cuts at: 0 892 952 1043 1184 2386 2406
Size: 892 60 91 141 1202 20
Fragments arranged by size:
1202 892 141 91 60 20
EcoICRI GAG'CTC
Cuts at: 0 150 1776 2406
Size: 150 1626 630
Fragments arranged by size:
1626 630 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 191 770 1165 1229 1530 1756 2179
Size: 175 16 579 395 64 301 226 423
Cuts at: 2179 2406
Size: 227
Fragments arranged by size:
579 423 395 301 227 226 175 64
16
EcoO109I rG'GnC_Cy
Cuts at: 0 159 2211 2406
Size: 159 2052 195
Fragments arranged by size:
2052 195 159
EcoRV GAT'ATC
Cuts at: 0 800 2406
Size: 800 1606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1560 2406
Size: 1560 846
HaeII r_GCGC'y
Cuts at: 0 32 2406
Size: 32 2374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 507 539 873 905 2406
Size: 507 32 334 32 1501
Fragments arranged by size:
1501 507 334 32 32
HincII GTy'rAC
Cuts at: 0 708 2406
Size: 708 1698
HindIII A'AGCT_T
Cuts at: 0 535 1349 1579 2406
Size: 535 814 230 827
Fragments arranged by size:
827 814 535 230
KasI G'GCGC_C
Cuts at: 0 28 2406
Size: 28 2378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1593 2406
Size: 453 1140 813
Fragments arranged by size:
1140 813 453
MscI TGG'CCA
Cuts at: 0 212 566 2406
Size: 212 354 1840
Fragments arranged by size:
1840 354 212
MslI CAynn'nnrTG
Cuts at: 0 1060 1547 1604 2138 2406
Size: 1060 487 57 534 268
Fragments arranged by size:
1060 534 487 268 57
MspA1I CmG'CkG
Cuts at: 0 23 34 1165 1591 2076 2406
Size: 23 11 1131 426 485 330
Fragments arranged by size:
1131 485 426 330 23 11
NarI GG'CG_CC
Cuts at: 0 29 2406
Size: 29 2377
NsiI A_TGCA'T
Cuts at: 0 1607 2406
Size: 1607 799
NspI r_CATG'y
Cuts at: 0 121 1605 2406
Size: 121 1484 801
Fragments arranged by size:
1484 801 121
PasI CC'CwG_GG
Cuts at: 0 1293 2406
Size: 1293 1113
PflMI CCAn_nnn'nTGG
Cuts at: 0 510 2406
Size: 510 1896
PfoI T'CCnGG_A
Cuts at: 0 311 1206 2406
Size: 311 895 1200
Fragments arranged by size:
1200 895 311
PmlI CAC'GTG
Cuts at: 0 919 2406
Size: 919 1487
PpuMI rG'GwC_Cy
Cuts at: 0 159 2406
Size: 159 2247
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 179 211 2406
Size: 179 32 2195
Fragments arranged by size:
2195 179 32
PstI C_TGCA'G
Cuts at: 0 2342 2406
Size: 2342 64
PvuII CAG'CTG
Cuts at: 0 1165 1591 2076 2406
Size: 1165 426 485 330
Fragments arranged by size:
1165 485 426 330
SacI G_AGCT'C
Cuts at: 0 152 1778 2406
Size: 152 1626 628
Fragments arranged by size:
1626 628 152
SacII CC_GC'GG
Cuts at: 0 35 2406
Size: 35 2371
SfcI C'TryA_G
Cuts at: 0 2338 2406
Size: 2338 68
SfoI GGC'GCC
Cuts at: 0 30 2406
Size: 30 2376
SmlI C'TyrA_G
Cuts at: 0 538 2040 2406
Size: 538 1502 366
Fragments arranged by size:
1502 538 366
SspI AAT'ATT
Cuts at: 0 546 812 1440 2406
Size: 546 266 628 966
Fragments arranged by size:
966 628 546 266
StyI C'CwwG_G
Cuts at: 0 162 1992 2406
Size: 162 1830 414
Fragments arranged by size:
1830 414 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 1892 2340 2406
Size: 712 1180 448 66
Fragments arranged by size:
1180 712 448 66
TatI w'GTAC_w
Cuts at: 0 1835 2406
Size: 1835 571
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 467 499 708 740 1062 1094 1995 2027
Size: 467 32 209 32 322 32 901 32
Cuts at: 2027 2114 2146 2406
Size: 87 32 260
Fragments arranged by size:
901 467 322 260 209 87 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 900 2406
Size: 900 1506
XmnI GAAnn'nnTTC
Cuts at: 0 1460 1543 2406
Size: 1460 83 863
Fragments arranged by size:
1460 863 83
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApaLI ApoI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BsrDI BsrGI BssSI BstAPI BstXI
BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NarI NsiI NspI PasI PflMI
PfoI PmlI PpuMI PsrI PstI PvuII SacI SacII
SfcI SfoI SmlI SspI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI AscI AseI AsiSI AvaI BaeI
BbvCI BcgI BglII BlpI BmrI BmtI Bpu10I BsaBI
BsiEI BsiWI BsmI BspMI BsrBI BsrFI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI
HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI
XmaI ZraI