################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BcgI BciVI BfrI BlnI BlpI Bpu1102I BsiWI BsmI BspDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MluI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1996 GACGTC GACGT^C AccI 1774 GTCTAC GT^[AC][GT]AC AspI 2302 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 674 TGCGCA TGC^GCA BamHI 1509 GGATCC G^GATCC BbrPI 948 CACGTG CAC^GTG BclI 812 TGATCA T^GATCA BglI 2121 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1562 AGATCT A^GATCT BsaBI 2466 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 349 GTGCAG GTGCAG(16/14) BsmBI 2724 CGTCTC CGTCTC(1/5) Bsp1407I 2606 TGTACA T^GTACA BspMI 1778 ACCTGC ACCTGC(4/8) BsrGI 2606 TGTACA T^GTACA BstAPI 944 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2435 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 2716 GACAACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2816 CGGCCG C^GGCCG EclXI 2816 CGGCCG C^GGCCG Eco52I 2816 CGGCCG C^GGCCG EcoRV 1702 GATATC GAT^ATC FbaI 812 TGATCA T^GATCA FspI 674 TGCGCA TGC^GCA MamI 2466 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2097 CAATTG C^AATTG MunI 2097 CAATTG C^AATTG NcoI 1505 CCATGG C^CATGG PflFI 2302 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 948 CACGTG CAC^GTG PmlI 948 CACGTG CAC^GTG SapI 2358 GCTCTTC GCTCTTC(1/4) ScaI 1004 AGTACT AGT^ACT SmaI 1865 CCCGGG CCC^GGG SphI 2648 GCATGC GCATG^C SspBI 2606 TGTACA T^GTACA Tth111I 2302 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 513 TCTAGA T^CTAGA XcmI 475 CCAGCAGCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1865 CCCGGG C^CCGGG XmaI 1865 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 57 TCCGGA T^CCGGA 2598 TCCGGA ApaI 2005 GGGCCC GGGCC^C 2053 GGGCCC AspHI 1033 GAGCTC G[AT]GC[AT]^C 2356 GAGCTC BbvCI 978 CCTCAGC CCTCAGC(-5/-2) 2281 GCTGAGG BseAI 57 TCCGGA T^CCGGA 2598 TCCGGA BsiHKAI 1033 GAGCTC G[AT]GC[AT]^C 2356 GAGCTC BspEI 57 TCCGGA T^CCGGA 2598 TCCGGA BspHI 869 TCATGA T^CATGA 956 TCATGA BspLU11I 881 ACATGT A^CATGT 1137 ACATGT BsrBI 1122 CCGCTC CCGCTC(-3/-3) 688 GAGCGG DsaI 1374 CCACGG C^C[GA][CT]GG 1505 CCATGG EcoNI 432 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2379 CCTGGAGAAGG MroI 57 TCCGGA T^CCGGA 2598 TCCGGA MseI 1247 TTAA T^TAA 2885 TTAA NaeI 292 GCCGGC GCC^GGC 2818 GCCGGC NgoMIV 292 GCCGGC G^CCGGC 2818 GCCGGC PflMI 1173 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2787 CCAGGACGTGG PpuMI 1241 AGGACCT [GA]G^G[AT]CC[CT] 2549 GGGACCT RcaI 869 TCATGA T^CATGA 956 TCATGA SacI 1033 GAGCTC GAGCT^C 2356 GAGCTC SexAI 494 ACCTGGT A^CC[AT]GGT 1091 ACCTGGT StuI 228 AGGCCT AGG^CCT 1079 AGGCCT Tru9I 1247 TTAA T^TAA 2885 TTAA Tsp509I 1175 AATT ^AATT 2098 AATT Van91I 1173 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2787 CCAGGACGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII