(Circular) (Six-base) MAPSORT of: pFN21AE3437.seq Check: 3881 from: 1 to: 7594 pFN21AE3437 7594 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4005 5404 278 Size: 53 83 186 936 2469 1399 2468 Fragments arranged by size: 2469 2468 1399 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3780 4937 1937 Size: 1843 1157 4594 Fragments arranged by size: 4594 1843 1157 Acc65I G'GTAC_C Cuts at: 4915 4915 Size: 7594 AclI AA'CG_TT Cuts at: 1563 5721 6094 1563 Size: 4158 373 3063 Fragments arranged by size: 4158 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3625 4227 5657 7041 7309 1632 Size: 1993 602 1430 1384 268 1917 Fragments arranged by size: 1993 1917 1430 1384 602 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7397 AflIII A'CryG_T Cuts at: 1203 2886 2952 3142 6493 7470 1203 Size: 1683 66 190 3351 977 1327 Fragments arranged by size: 3351 1683 1327 977 190 66 AgeI A'CCGG_T Cuts at: 6425 6425 Size: 7594 AhdI GACnn_n'nnGTC Cuts at: 6323 6323 Size: 7594 AleI CACnn'nnGTG Cuts at: 1166 3401 4373 4535 1166 Size: 2235 972 162 4225 Fragments arranged by size: 4225 2235 972 162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7594 AlwNI CAG_nnn'CTG Cuts at: 2162 2486 3214 5337 6909 2162 Size: 324 728 2123 1572 2847 Fragments arranged by size: 2847 2123 1572 728 324 ApaI G_GGCC'C Cuts at: 4014 4062 4014 Size: 48 7546 ApaLI G'TGCA_C Cuts at: 5651 6807 5651 Size: 1156 6438 ApoI r'AATT_y Cuts at: 1493 4903 5129 5281 7390 1493 Size: 3410 226 152 2109 1697 Fragments arranged by size: 3410 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6148 160 Size: 872 5116 1606 Fragments arranged by size: 5116 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7594 AvaI C'yCGr_G Cuts at: 1870 1943 2688 3870 4515 4919 1870 Size: 73 745 1182 645 404 4545 Fragments arranged by size: 4545 1182 745 645 404 73 BamHI G'GATC_C Cuts at: 3514 4924 5291 3514 Size: 1410 367 5817 Fragments arranged by size: 5817 1410 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2433 4083 4835 4915 Size: 325 217 627 463 183 1650 752 80 Cuts at: 4915 6370 618 Size: 1455 1842 Fragments arranged by size: 1842 1650 1455 752 627 463 325 217 183 80 BanII G_rGCy'C Cuts at: 1391 2037 2712 3042 3755 4014 4062 4365 4913 Size: 646 675 330 713 259 48 303 548 Cuts at: 4913 1391 Size: 4072 Fragments arranged by size: 4072 713 675 646 548 330 303 259 48 BbeI G_GCGC'C Cuts at: 2254 2437 4839 2254 Size: 183 2402 5009 Fragments arranged by size: 5009 2402 183 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2078 2724 3490 3631 928 Size: 971 179 646 766 141 4891 Fragments arranged by size: 4891 971 766 646 179 141 BbvCI CC'TCA_GC Cuts at: 2984 4287 2984 Size: 1303 6291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5785 5819 1903 Size: 34 3848 34 3678 Fragments arranged by size: 3848 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5486 6696 5486 Size: 1210 6384 BclI T'GATC_A Cuts at: 1548 2817 1548 Size: 1269 6325 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4132 6205 136 Size: 107 122 71 631 605 2460 2073 1525 Fragments arranged by size: 2460 2073 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3567 7589 3567 Size: 4022 3572 BlpI GC'TnA_GC Cuts at: 5011 5011 Size: 7594 Bme1580I G_kGCm'C Cuts at: 1790 3410 3870 4014 4062 4088 5655 6811 1790 Size: 1620 460 144 48 26 1567 1156 2573 Fragments arranged by size: 2573 1620 1567 1156 460 144 48 26 BmgBI CAC'GTC Cuts at: 1318 4798 1318 Size: 3480 4114 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2585 2817 3068 Size: 416 53 83 399 24 1168 232 251 Cuts at: 3068 4029 4252 5325 6283 442 Size: 961 223 1073 958 1753 Fragments arranged by size: 1753 1168 1073 961 958 416 399 251 232 223 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7594 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2359 3053 4089 4406 6254 1239 Size: 1120 694 1036 317 1848 2579 Fragments arranged by size: 2579 1848 1120 1036 694 317 Bpu10I CC'TnA_GC Cuts at: 2984 4041 4287 2984 Size: 1057 246 6291 Fragments arranged by size: 6291 1057 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2423 2822 3739 5719 6584 6882 2423 Size: 399 917 1980 865 298 3135 Fragments arranged by size: 3135 1980 917 865 399 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2822 4029 4373 6257 882 Size: 617 1323 1207 344 1884 2219 Fragments arranged by size: 2219 1884 1323 1207 617 344 BsaAI yAC'GTr Cuts at: 493 1204 2353 2955 3931 4297 4321 5385 493 Size: 711 1149 602 976 366 24 1064 2702 Fragments arranged by size: 2702 1149 1064 976 711 602 366 24 BsaBI GATnn'nnATC Cuts at: 4475 7588 4475 Size: 3113 4481 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2083 2251 2434 4002 Size: 53 83 186 936 550 168 183 1568 Cuts at: 4002 4807 4836 5401 5783 6418 275 Size: 805 29 565 382 635 1451 Fragments arranged by size: 1568 1451 936 805 635 565 550 382 186 183 168 83 53 29 BsaWI w'CCGG_w Cuts at: 2062 3304 4603 6027 6425 6699 6846 7257 2062 Size: 1242 1299 1424 398 274 147 411 2399 Fragments arranged by size: 2399 1424 1299 1242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2357 2387 3113 3143 3203 Size: 30 165 30 954 30 726 30 60 Cuts at: 3203 3233 3803 3833 3837 3867 3881 3911 4715 Size: 30 570 30 4 30 14 30 804 Cuts at: 4715 4745 1178 Size: 30 4027 Fragments arranged by size: 4027 954 804 726 570 165 60 30 30 30 30 30 30 30 30 30 14 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 3050 3689 4001 1186 Size: 1051 813 639 312 4779 Fragments arranged by size: 4779 1051 813 639 312 BseYI C'CCAG_C Cuts at: 2479 2855 3296 4055 4061 4221 4840 4868 6797 Size: 376 441 759 6 160 619 28 1929 Cuts at: 6797 7293 2479 Size: 496 2780 Fragments arranged by size: 2780 1929 759 619 496 441 376 160 28 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 1163 Size: 573 639 6382 Fragments arranged by size: 6382 639 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2145 3305 4192 4824 5044 5805 Size: 275 186 144 1160 887 632 220 761 Cuts at: 5805 6833 1540 Size: 1028 2301 Fragments arranged by size: 2301 1160 1028 887 761 632 275 220 186 144 BsiHKAI G_wGCw'C Cuts at: 3042 4365 5655 5740 6811 3042 Size: 1323 1290 85 1071 3825 Fragments arranged by size: 3825 1323 1290 1071 85 BsmI GAATG_Cn' Cuts at: 5105 5198 5105 Size: 93 7501 BsmBI CGTCTCn'nnnn_ Cuts at: 4735 4735 Size: 7594 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2712 3042 3410 3755 3870 4014 Size: 399 247 675 330 368 345 115 144 Cuts at: 4014 4062 4088 4365 4913 5655 5740 6811 1391 Size: 48 26 277 548 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 675 548 399 368 345 330 277 247 144 115 85 48 26 BspEI T'CCGG_A Cuts at: 2062 4603 7257 2062 Size: 2541 2654 2399 Fragments arranged by size: 2654 2541 2399 BspHI T'CATG_A Cuts at: 2874 2961 5483 7213 2874 Size: 87 2522 1730 3255 Fragments arranged by size: 3255 2522 1730 87 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3792 4949 844 Size: 2948 1157 3489 Fragments arranged by size: 3489 2948 1157 BsrBI CCG'CTC Cuts at: 1394 1823 2695 3129 5481 1394 Size: 429 872 434 2352 3507 Fragments arranged by size: 3507 2352 872 434 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2995 3427 4230 6089 6263 65 Size: 1183 1747 432 803 1859 174 1396 Fragments arranged by size: 1859 1747 1396 1183 803 432 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2297 3540 3915 4823 6238 6425 1700 Size: 110 487 1243 375 908 1415 187 2869 Fragments arranged by size: 2869 1415 1243 908 487 375 187 110 BsrGI T'GTAC_A Cuts at: 96 4611 96 Size: 4515 3079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7594 BssSI C'ACGA_G Cuts at: 2219 3545 4535 5654 6666 2219 Size: 1326 990 1119 1012 3147 Fragments arranged by size: 3147 1326 1119 1012 990 BstAPI GCAn_nnn'nTGC Cuts at: 794 2955 794 Size: 2161 5433 BstBI TT'CG_AA Cuts at: 6398 6398 Size: 7594 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7594 BstXI CCAn_nnnn'nTGG Cuts at: 4447 4447 Size: 7594 BstYI r'GATC_y Cuts at: 1972 3514 3567 3574 4924 5291 5676 5693 7145 Size: 1542 53 7 1350 367 385 17 1452 Cuts at: 7145 7231 7243 7589 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1542 1452 1350 385 367 346 86 53 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 2004 3379 3510 513 Size: 552 939 1375 131 4597 Fragments arranged by size: 4597 1375 939 552 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2147 3303 4612 487 Size: 683 56 921 1156 1309 3469 Fragments arranged by size: 3469 1309 1156 921 683 56 BtsI GCAGTG_nn' Cuts at: 2288 2345 2984 3464 5110 5904 5924 2288 Size: 57 639 480 1646 794 20 3958 Fragments arranged by size: 3958 1646 794 639 480 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7594 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7559 DraI TTT'AAA Cuts at: 4898 4898 Size: 7594 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4727 6601 809 Size: 507 3411 1874 1802 Fragments arranged by size: 3411 1874 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2527 3118 3163 Size: 54 1425 50 275 18 697 591 45 Cuts at: 3163 4699 4821 5041 5930 7559 8 Size: 1536 122 220 889 1629 43 Fragments arranged by size: 1629 1536 1425 889 697 591 275 220 122 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4821 5041 1537 Size: 275 3009 220 4090 Fragments arranged by size: 4090 3009 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 2113 2889 3392 4370 4784 5524 1353 Size: 760 776 503 978 414 740 3423 Fragments arranged by size: 3423 978 776 760 740 503 414 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3364 6181 6555 6701 387 Size: 1064 138 1775 2817 374 146 1280 Fragments arranged by size: 2817 1775 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3040 4363 3040 Size: 1323 6271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2359 3053 3625 4089 4227 4406 5657 Size: 393 727 694 572 464 138 179 1251 Cuts at: 5657 6254 7041 7309 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1251 787 727 694 597 572 464 393 268 179 138 EcoNI CCTnn'n_nnAGG Cuts at: 2441 4388 2441 Size: 1947 5647 EcoO109I rG'GnC_Cy Cuts at: 3247 3892 4011 4555 3247 Size: 645 119 544 6286 Fragments arranged by size: 6286 645 544 119 EcoRI G'AATT_C Cuts at: 4903 5281 4903 Size: 378 7216 EcoRV GAT'ATC Cuts at: 3709 3709 Size: 7594 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3505 3505 Size: 7594 FspI TGC'GCA Cuts at: 2681 6100 2681 Size: 3419 4175 HaeII r_GCGC'y Cuts at: 1678 2254 2437 4839 6741 1678 Size: 576 183 2402 1902 2531 Fragments arranged by size: 2531 2402 1902 576 183 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2357 2389 3203 3235 3559 Size: 32 135 32 785 32 814 32 324 Cuts at: 3559 3591 3750 3782 3837 3869 6235 6267 6309 Size: 32 159 32 55 32 2366 32 42 Cuts at: 6309 6341 1373 Size: 32 2626 Fragments arranged by size: 2626 2366 814 785 324 159 135 55 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4938 5184 669 Size: 988 281 3000 246 3079 Fragments arranged by size: 3079 3000 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7594 HpaI GTT'AAC Cuts at: 5184 5184 Size: 7594 KasI G'GCGC_C Cuts at: 2250 2433 4835 2250 Size: 183 2402 5009 Fragments arranged by size: 5009 2402 183 KpnI G_GTAC'C Cuts at: 4919 4919 Size: 7594 MfeI C'AATT_G Cuts at: 4102 5193 4102 Size: 1091 6503 MluI A'CGCG_T Cuts at: 7470 7470 Size: 7594 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2871 4662 6708 6892 1305 Size: 1566 1791 2046 184 2007 Fragments arranged by size: 2046 2007 1791 1566 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2529 3120 3165 10 Size: 54 1425 343 697 591 45 4439 Fragments arranged by size: 4439 1425 697 591 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 3401 4120 4373 4535 5552 5911 6070 Size: 648 2235 719 253 162 1017 359 159 Cuts at: 6070 518 Size: 2042 Fragments arranged by size: 2235 2042 1017 719 648 359 253 162 159 MspA1I CmG'CkG Cuts at: 1606 2103 2159 2189 2486 2508 2866 4150 4432 Size: 497 56 30 297 22 358 1284 282 Cuts at: 4432 4698 5010 5687 6835 7080 1606 Size: 266 312 677 1148 245 2120 Fragments arranged by size: 2120 1284 1148 677 497 358 312 297 282 266 245 56 30 22 NaeI GCC'GGC Cuts at: 2299 4825 2299 Size: 2526 5068 NarI GG'CG_CC Cuts at: 2251 2434 4836 2251 Size: 183 2402 5009 Fragments arranged by size: 5009 2402 183 NcoI C'CATG_G Cuts at: 513 1065 2004 3510 513 Size: 552 939 1506 4597 Fragments arranged by size: 4597 1506 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7594 NgoMIV G'CCGG_C Cuts at: 2297 4823 2297 Size: 2526 5068 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7594 NotI GC'GGCC_GC Cuts at: 5041 5041 Size: 7594 NspI r_CATG'y Cuts at: 1132 1229 2873 2890 3146 4657 4952 6497 1132 Size: 97 1644 17 256 1511 295 1545 2229 Fragments arranged by size: 2229 1644 1545 1511 295 256 97 17 PasI CC'CwG_GG Cuts at: 1348 4007 1348 Size: 2659 4935 PciI A'CATG_T Cuts at: 2886 3142 6493 2886 Size: 256 3351 3987 Fragments arranged by size: 3987 3351 256 PflMI CCAn_nnn'nTGG Cuts at: 1108 3184 4798 1108 Size: 2076 1614 3904 Fragments arranged by size: 3904 2076 1614 PfoI T'CCnGG_A Cuts at: 2049 3228 4095 4551 2049 Size: 1179 867 456 5092 Fragments arranged by size: 5092 1179 867 456 PmeI GTTT'AAAC Cuts at: 4898 4898 Size: 7594 PmlI CAC'GTG Cuts at: 2955 2955 Size: 7594 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5663 5695 7176 7208 5663 Size: 32 1481 32 6049 Fragments arranged by size: 6049 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3247 4555 3247 Size: 1308 6286 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7594 PsiI TTA'TAA Cuts at: 5164 5164 Size: 7594 PspOMI G'GGCC_C Cuts at: 4010 4058 4010 Size: 48 7546 PstI C_TGCA'G Cuts at: 830 2979 4164 4350 4859 4946 830 Size: 2149 1185 186 509 87 3478 Fragments arranged by size: 3478 2149 1185 509 186 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7594 PvuII CAG'CTG Cuts at: 2103 2159 2486 2508 2866 4150 4432 2103 Size: 56 327 22 358 1284 282 5265 Fragments arranged by size: 5265 1284 358 327 282 56 22 SacI G_AGCT'C Cuts at: 3042 4365 3042 Size: 1323 6271 SalI G'TCGA_C Cuts at: 1936 4936 1936 Size: 3000 4594 SapI GCTCTTCn'nnn_ Cuts at: 4370 4370 Size: 7594 SbfI CC_TGCA'GG Cuts at: 4946 4946 Size: 7594 ScaI AGT'ACT Cuts at: 3011 5842 7554 3011 Size: 2831 1712 3051 Fragments arranged by size: 3051 2831 1712 SexAI A'CCwGG_T Cuts at: 2499 3096 2499 Size: 597 6997 SfcI C'TryA_G Cuts at: 826 1045 2113 2975 4160 4346 4855 4942 6077 Size: 219 1068 862 1185 186 509 87 1135 Cuts at: 6077 6758 6949 826 Size: 681 191 1471 Fragments arranged by size: 1471 1185 1135 1068 862 681 509 219 191 186 87 SfoI GGC'GCC Cuts at: 2252 2435 4837 2252 Size: 183 2402 5009 Fragments arranged by size: 5009 2402 183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7594 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7594 SmaI CCC'GGG Cuts at: 1872 3872 4921 1872 Size: 2000 1049 4545 Fragments arranged by size: 4545 2000 1049 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 2801 3754 5698 6599 6861 Size: 197 926 495 363 953 1944 901 262 Cuts at: 6861 820 Size: 1553 Fragments arranged by size: 1944 1553 953 926 901 495 363 262 197 SnaBI TAC'GTA Cuts at: 493 5385 493 Size: 4892 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7594 SphI G_CATG'C Cuts at: 1132 4657 4952 1132 Size: 3525 295 3774 Fragments arranged by size: 3774 3525 295 SspI AAT'ATT Cuts at: 5 52 5518 5 Size: 47 5466 2081 Fragments arranged by size: 5466 2081 47 StuI AGG'CCT Cuts at: 1517 2235 3086 1517 Size: 718 851 6025 Fragments arranged by size: 6025 851 718 StyI C'CwwG_G Cuts at: 513 1065 2004 3510 4108 4130 4761 5033 513 Size: 552 939 1506 598 22 631 272 3074 Fragments arranged by size: 3074 1506 939 631 598 552 272 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2551 4882 5621 5638 5791 5976 6421 Size: 1255 971 2331 739 17 153 185 445 Cuts at: 6421 325 Size: 1498 Fragments arranged by size: 2331 1498 1255 971 739 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3009 4611 5840 Size: 274 80 33 51 1443 1032 1602 1229 Cuts at: 5840 7552 96 Size: 1712 138 Fragments arranged by size: 1712 1602 1443 1229 1032 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2231 2263 3113 3145 7554 7586 2231 Size: 32 850 32 4409 32 2239 Fragments arranged by size: 4409 2239 850 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4310 1316 Size: 2994 4600 XbaI T'CTAG_A Cuts at: 2518 4930 2518 Size: 2412 5182 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2487 2487 Size: 7594 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7594 XmaI C'CCGG_G Cuts at: 1870 3870 4919 1870 Size: 2000 1049 4545 Fragments arranged by size: 4545 2000 1049 XmnI GAAnn'nnTTC Cuts at: 5723 5723 Size: 7594 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4003 5402 276 Size: 53 83 186 936 2469 1399 2468 Fragments arranged by size: 2469 2468 1399 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI