(Linear) (Six-base) MAPSORT of: KIEE3437.seq Check: 9173 from: 1 to: 2889 KIEE3437.orf With 182 enzymes: SgfI * July 3, 2012 17:51 .. AatII G_ACGT'C Cuts at: 0 2000 2889 Size: 2000 889 AccI GT'mk_AC Cuts at: 0 1775 2889 Size: 1775 1114 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1620 2222 2889 Size: 1620 602 667 Fragments arranged by size: 1620 667 602 AflIII A'CryG_T Cuts at: 0 881 947 1137 2889 Size: 881 66 190 1752 Fragments arranged by size: 1752 881 190 66 AleI CACnn'nnGTG Cuts at: 0 1396 2368 2530 2889 Size: 1396 972 162 359 Fragments arranged by size: 1396 972 359 162 AlwNI CAG_nnn'CTG Cuts at: 0 157 481 1209 2889 Size: 157 324 728 1680 Fragments arranged by size: 1680 728 324 157 ApaI G_GGCC'C Cuts at: 0 2009 2057 2889 Size: 2009 48 832 Fragments arranged by size: 2009 832 48 AvaI C'yCGr_G Cuts at: 0 683 1865 2510 2889 Size: 683 1182 645 379 Fragments arranged by size: 1182 683 645 379 BamHI G'GATC_C Cuts at: 0 1509 2889 Size: 1509 1380 BanI G'GyrC_C Cuts at: 0 245 428 2078 2830 2889 Size: 245 183 1650 752 59 Fragments arranged by size: 1650 752 245 183 59 BanII G_rGCy'C Cuts at: 0 32 707 1037 1750 2009 2057 2360 2889 Size: 32 675 330 713 259 48 303 529 Fragments arranged by size: 713 675 529 330 303 259 48 32 BbeI G_GCGC'C Cuts at: 0 249 432 2834 2889 Size: 249 183 2402 55 Fragments arranged by size: 2402 249 183 55 BbsI GAAGACnn'nnnn_ Cuts at: 0 73 719 1485 1626 2889 Size: 73 646 766 141 1263 Fragments arranged by size: 1263 766 646 141 73 BbvCI CC'TCA_GC Cuts at: 0 979 2282 2889 Size: 979 1303 607 Fragments arranged by size: 1303 979 607 BclI T'GATC_A Cuts at: 0 812 2889 Size: 812 2077 BglI GCCn_nnn'nGGC Cuts at: 0 2127 2889 Size: 2127 762 BglII A'GATC_T Cuts at: 0 1562 2889 Size: 1562 1327 Bme1580I G_kGCm'C Cuts at: 0 1405 1865 2009 2057 2083 2889 Size: 1405 460 144 48 26 806 Fragments arranged by size: 1405 806 460 144 48 26 BmgBI CAC'GTC Cuts at: 0 2793 2889 Size: 2793 96 BmrI ACTGGGnnnn_n' Cuts at: 0 580 812 1063 2024 2247 2889 Size: 580 232 251 961 223 642 Fragments arranged by size: 961 642 580 251 232 223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 354 1048 2084 2401 2889 Size: 354 694 1036 317 488 Fragments arranged by size: 1036 694 488 354 317 Bpu10I CC'TnA_GC Cuts at: 0 979 2036 2282 2889 Size: 979 1057 246 607 Fragments arranged by size: 1057 979 607 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 817 1734 2889 Size: 418 399 917 1155 Fragments arranged by size: 1155 917 418 399 BsaI GGTCTCn'nnnn_ Cuts at: 0 817 2024 2368 2889 Size: 817 1207 344 521 Fragments arranged by size: 1207 817 521 344 BsaAI yAC'GTr Cuts at: 0 348 950 1926 2292 2316 2889 Size: 348 602 976 366 24 573 Fragments arranged by size: 976 602 573 366 348 24 BsaBI GATnn'nnATC Cuts at: 0 2470 2889 Size: 2470 419 BsaHI Gr'CG_yC Cuts at: 0 78 246 429 1997 2802 2831 2889 Size: 78 168 183 1568 805 29 58 Fragments arranged by size: 1568 805 183 168 78 58 29 BsaWI w'CCGG_w Cuts at: 0 57 1299 2598 2889 Size: 57 1242 1299 291 Fragments arranged by size: 1299 1242 291 57 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 352 382 1108 1138 1198 1228 1798 1828 Size: 352 30 726 30 60 30 570 30 Cuts at: 1828 1832 1862 1876 1906 2710 2740 2889 Size: 4 30 14 30 804 30 149 Fragments arranged by size: 804 726 570 352 149 60 30 30 30 30 30 30 30 14 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 232 1045 1684 1996 2889 Size: 232 813 639 312 893 Fragments arranged by size: 893 813 639 312 232 BseYI C'CCAG_C Cuts at: 0 474 850 1291 2050 2056 2216 2835 2863 Size: 474 376 441 759 6 160 619 28 Cuts at: 2863 2889 Size: 26 Fragments arranged by size: 759 619 474 441 376 160 28 26 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 370 2889 Size: 370 2519 BsiEI CG_ry'CG Cuts at: 0 140 1300 2187 2819 2889 Size: 140 1160 887 632 70 Fragments arranged by size: 1160 887 632 140 70 BsiHKAI G_wGCw'C Cuts at: 0 1037 2360 2889 Size: 1037 1323 529 Fragments arranged by size: 1323 1037 529 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2730 2889 Size: 2730 159 Bsp1286I G_dGCh'C Cuts at: 0 32 707 1037 1405 1750 1865 2009 2057 Size: 32 675 330 368 345 115 144 48 Cuts at: 2057 2083 2360 2889 Size: 26 277 529 Fragments arranged by size: 675 529 368 345 330 277 144 115 48 32 26 BspEI T'CCGG_A Cuts at: 0 57 2598 2889 Size: 57 2541 291 Fragments arranged by size: 2541 291 57 BspHI T'CATG_A Cuts at: 0 869 956 2889 Size: 869 87 1933 Fragments arranged by size: 1933 869 87 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1787 2889 Size: 1787 1102 BsrBI CCG'CTC Cuts at: 0 690 1124 2889 Size: 690 434 1765 Fragments arranged by size: 1765 690 434 BsrDI GCAATG_nn' Cuts at: 0 990 1422 2225 2889 Size: 990 432 803 664 Fragments arranged by size: 990 803 664 432 BsrFI r'CCGG_y Cuts at: 0 292 1535 1910 2818 2889 Size: 292 1243 375 908 71 Fragments arranged by size: 1243 908 375 292 71 BsrGI T'GTAC_A Cuts at: 0 2606 2889 Size: 2606 283 BssSI C'ACGA_G Cuts at: 0 214 1540 2530 2889 Size: 214 1326 990 359 Fragments arranged by size: 1326 990 359 214 BstAPI GCAn_nnn'nTGC Cuts at: 0 950 2889 Size: 950 1939 BstXI CCAn_nnnn'nTGG Cuts at: 0 2442 2889 Size: 2442 447 BstYI r'GATC_y Cuts at: 0 1509 1562 1569 2889 Size: 1509 53 7 1320 Fragments arranged by size: 1509 1320 53 7 BtgI C'CryG_G Cuts at: 0 1374 1505 2889 Size: 1374 131 1384 Fragments arranged by size: 1384 1374 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 142 1298 2607 2889 Size: 142 1156 1309 282 Fragments arranged by size: 1309 1156 282 142 BtsI GCAGTG_nn' Cuts at: 0 283 340 979 1459 2889 Size: 283 57 639 480 1430 Fragments arranged by size: 1430 639 480 283 57 DrdI GACnn_nn'nnGTC Cuts at: 0 2722 2889 Size: 2722 167 EaeI y'GGCC_r Cuts at: 0 522 1113 1158 2694 2816 2889 Size: 522 591 45 1536 122 73 Fragments arranged by size: 1536 591 522 122 73 45 EagI C'GGCC_G Cuts at: 0 2816 2889 Size: 2816 73 EarI CTCTTCn'nnn_ Cuts at: 0 108 884 1387 2365 2779 2889 Size: 108 776 503 978 414 110 Fragments arranged by size: 978 776 503 414 110 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1359 2889 Size: 1359 1530 EcoICRI GAG'CTC Cuts at: 0 1035 2358 2889 Size: 1035 1323 531 Fragments arranged by size: 1323 1035 531 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 354 1048 1620 2084 2222 2401 2889 Size: 354 694 572 464 138 179 488 Fragments arranged by size: 694 572 488 464 354 179 138 EcoNI CCTnn'n_nnAGG Cuts at: 0 436 2383 2889 Size: 436 1947 506 Fragments arranged by size: 1947 506 436 EcoO109I rG'GnC_Cy Cuts at: 0 1242 1887 2006 2550 2889 Size: 1242 645 119 544 339 Fragments arranged by size: 1242 645 544 339 119 EcoRV GAT'ATC Cuts at: 0 1704 2889 Size: 1704 1185 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1500 2889 Size: 1500 1389 FspI TGC'GCA Cuts at: 0 676 2889 Size: 676 2213 HaeII r_GCGC'y Cuts at: 0 249 432 2834 2889 Size: 249 183 2402 55 Fragments arranged by size: 2402 249 183 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 352 384 1198 1230 1554 1586 1745 1777 Size: 352 32 814 32 324 32 159 32 Cuts at: 1777 1832 1864 2889 Size: 55 32 1025 Fragments arranged by size: 1025 814 352 324 159 55 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 245 428 2830 2889 Size: 245 183 2402 59 Fragments arranged by size: 2402 245 183 59 MfeI C'AATT_G Cuts at: 0 2097 2889 Size: 2097 792 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 866 2657 2889 Size: 866 1791 232 Fragments arranged by size: 1791 866 232 MscI TGG'CCA Cuts at: 0 524 1115 1160 2889 Size: 524 591 45 1729 Fragments arranged by size: 1729 591 524 45 MslI CAynn'nnrTG Cuts at: 0 1396 2115 2368 2530 2889 Size: 1396 719 253 162 359 Fragments arranged by size: 1396 719 359 253 162 MspA1I CmG'CkG Cuts at: 0 98 154 184 481 503 861 2145 2427 Size: 98 56 30 297 22 358 1284 282 Cuts at: 2427 2693 2889 Size: 266 196 Fragments arranged by size: 1284 358 297 282 266 196 98 56 30 22 NaeI GCC'GGC Cuts at: 0 294 2820 2889 Size: 294 2526 69 Fragments arranged by size: 2526 294 69 NarI GG'CG_CC Cuts at: 0 246 429 2831 2889 Size: 246 183 2402 58 Fragments arranged by size: 2402 246 183 58 NcoI C'CATG_G Cuts at: 0 1505 2889 Size: 1505 1384 NgoMIV G'CCGG_C Cuts at: 0 292 2818 2889 Size: 292 2526 71 Fragments arranged by size: 2526 292 71 NspI r_CATG'y Cuts at: 0 868 885 1141 2652 2889 Size: 868 17 256 1511 237 Fragments arranged by size: 1511 868 256 237 17 PasI CC'CwG_GG Cuts at: 0 2002 2889 Size: 2002 887 PciI A'CATG_T Cuts at: 0 881 1137 2889 Size: 881 256 1752 Fragments arranged by size: 1752 881 256 PflMI CCAn_nnn'nTGG Cuts at: 0 1179 2793 2889 Size: 1179 1614 96 Fragments arranged by size: 1614 1179 96 PfoI T'CCnGG_A Cuts at: 0 44 1223 2090 2546 2889 Size: 44 1179 867 456 343 Fragments arranged by size: 1179 867 456 343 44 PmlI CAC'GTG Cuts at: 0 950 2889 Size: 950 1939 PpuMI rG'GwC_Cy Cuts at: 0 1242 2550 2889 Size: 1242 1308 339 Fragments arranged by size: 1308 1242 339 PspOMI G'GGCC_C Cuts at: 0 2005 2053 2889 Size: 2005 48 836 Fragments arranged by size: 2005 836 48 PstI C_TGCA'G Cuts at: 0 974 2159 2345 2854 2889 Size: 974 1185 186 509 35 Fragments arranged by size: 1185 974 509 186 35 PvuII CAG'CTG Cuts at: 0 98 154 481 503 861 2145 2427 2889 Size: 98 56 327 22 358 1284 282 462 Fragments arranged by size: 1284 462 358 327 282 98 56 22 SacI G_AGCT'C Cuts at: 0 1037 2360 2889 Size: 1037 1323 529 Fragments arranged by size: 1323 1037 529 SapI GCTCTTCn'nnn_ Cuts at: 0 2365 2889 Size: 2365 524 ScaI AGT'ACT Cuts at: 0 1006 2889 Size: 1006 1883 SexAI A'CCwGG_T Cuts at: 0 494 1091 2889 Size: 494 597 1798 Fragments arranged by size: 1798 597 494 SfcI C'TryA_G Cuts at: 0 108 970 2155 2341 2850 2889 Size: 108 862 1185 186 509 39 Fragments arranged by size: 1185 862 509 186 108 39 SfoI GGC'GCC Cuts at: 0 247 430 2832 2889 Size: 247 183 2402 57 Fragments arranged by size: 2402 247 183 57 SmaI CCC'GGG Cuts at: 0 1867 2889 Size: 1867 1022 SmlI C'TyrA_G Cuts at: 0 433 796 1749 2889 Size: 433 363 953 1140 Fragments arranged by size: 1140 953 433 363 SphI G_CATG'C Cuts at: 0 2652 2889 Size: 2652 237 StuI AGG'CCT Cuts at: 0 230 1081 2889 Size: 230 851 1808 Fragments arranged by size: 1808 851 230 StyI C'CwwG_G Cuts at: 0 1505 2103 2125 2756 2889 Size: 1505 598 22 631 133 Fragments arranged by size: 1505 631 598 133 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 546 2877 2889 Size: 546 2331 12 Fragments arranged by size: 2331 546 12 TatI w'GTAC_w Cuts at: 0 1004 2606 2889 Size: 1004 1602 283 Fragments arranged by size: 1602 1004 283 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 226 258 1108 1140 2889 Size: 226 32 850 32 1749 Fragments arranged by size: 1749 850 226 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2305 2889 Size: 2305 584 XbaI T'CTAG_A Cuts at: 0 513 2889 Size: 513 2376 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 482 2889 Size: 482 2407 XmaI C'CCGG_G Cuts at: 0 1865 2889 Size: 1865 1024 ZraI GAC'GTC Cuts at: 0 1998 2889 Size: 1998 891 Enzymes that do cut: AatII AccI AcuI AflIII AleI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BlpI BmtI BplI BsiWI BsmI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoRI FseI FspAI HincII HindIII HpaI KpnI MluI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XhoI XmnI