################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeIII HapII HgaI Hin1I HpaII KasI KpnI MamI MluI MluNI MroI MscI MspI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 40 CGCG CG^CG AflII 406 CTTAAG C^TTAAG Alw44I 1648 GTGCAC G^TGCAC AlwNI 812 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1648 GTGCAC G^TGCAC Asp700I 1170 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1648 GTGCAC G[AT]GC[AT]^C BbvCI 1444 GCTGAGG CCTCAGC(-5/-2) BcgI 541 CGAAGCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 406 CTTAAG C^TTAAG BseRI 1301 CTCCTC GAGGAG(10/8) BsiHKAI 1648 GTGCAC G[AT]GC[AT]^C BsiYI 1460 CCGCAGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1460 CCGCAGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 241 GAATGC GAATGC(1/-1) BspMI 1644 GCAGGT ACCTGC(4/8) BssKI 1166 CCAGG ^CC[ACGT]GG BstEII 1307 GGTCACC G^GT[ACGT]ACC BstNI 1166 CCAGG CC^[AT]GG BstPI 1307 GGTCACC G^GT[ACGT]ACC BstUI 40 CGCG CG^CG BstXI 259 CCATGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 355 AGATCC [GA]^GATC[CT] CfoI 72 GCGC GCG^C DraI 1125 TTTAAA TTT^AAA DraII 535 AGGACCC [GA]G^G[ACGT]CC[CT] EarI 753 GAAGAG CTCTTC(1/4) EcoO109I 535 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 1307 GGTCACC G^GT[ACGT]ACC EcoRII 1166 CCAGG ^CC[AT]GG EcoT14I 1348 CCATGG C^C[AT][AT]GG FauI 87 CCCGC CCCGC(4/6) HaeII 71 AGCGCC [GA]GCGC^[CT] HhaI 72 GCGC GCG^C HinP1I 72 GCGC G^CGC HincII 667 GTTAAC GT[CT]^[GA]AC HindII 667 GTTAAC GT[CT]^[GA]AC HindIII 1354 AAGCTT A^AGCTT HpaI 667 GTTAAC GTT^AAC Ksp632I 753 GAAGAG CTCTTC(1/4) KspI 39 CCGCGG CCGC^GG MfeI 1574 CAATTG C^AATTG MflI 355 AGATCC [GA]^GATC[CT] MunI 1574 CAATTG C^AATTG MvaI 1166 CCAGG CC^[AT]GG MvnI 40 CGCG CG^CG NcoI 1348 CCATGG C^CATGG NspI 883 GCATGT [GA]CATG^[CT] PpuMI 535 AGGACCC [GA]G^G[AT]CC[CT] PvuII 1442 CAGCTG CAG^CTG SacII 39 CCGCGG CCGC^GG SapI 753 GAAGAGC GCTCTTC(1/4) ScrFI 1166 CCAGG CC^[ACGT]GG SmlI 406 CTTAAG C^T[CT][GA]AG StyI 1348 CCATGG C^C[AT][AT]GG XhoII 355 AGATCC [GA]^GATC[CT] XmnI 1170 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 324 GTAC GT^AC 433 GTAC AocI 531 CCTCAGG CC^T[ACGT]AGG 1388 CCTCAGG BbsI 300 GAAGAC GAAGAC(2/6) 1657 GAAGAC BfaI 65 CTAG C^TAG 76 CTAG BmyI 3 GGGCAC G[AGT]GC[ACT]^C 1648 GTGCAC BpuAI 300 GAAGAC GAAGAC(2/6) 1657 GAAGAC BsaI 892 GAGACC GGTCTC(1/5) 1501 GGTCTC Bsp1286I 3 GGGCAC G[AGT]GC[ACT]^C 1648 GTGCAC Bsu36I 531 CCTCAGG CC^T[ACGT]AGG 1388 CCTCAGG DsaI 39 CCGCGG C^C[GA][CT]GG 1348 CCATGG Eco81I 531 CCTCAGG CC^T[ACGT]AGG 1388 CCTCAGG MaeI 65 CTAG C^TAG 76 CTAG MaeII 1131 ACGT A^CGT 1275 ACGT MslI 260 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 385 CACAAAAATG MspA1I 39 CCGCGG C[AC]G^C[GT]G 1442 CAGCTG MwoI 1444 GCTGAGGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1453 GCAAGAACCGC PstI 230 CTGCAG CTGCA^G 471 CTGCAG RsaI 324 GTAC GT^AC 433 GTAC SfcI 230 CTGCAG C^T[GA][CT]AG 471 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BbvI BciVI BpmI BsaJI BsmAI BsmFI BspHI BsrI BstF5I Cfr13I DdeI DpnII Fnu4HI FokI HinfI HphI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIII NlaIV PleI RcaI Sau3AI Sau96I SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI