################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## Acc65I AccI AccIII AcsI AfaI AgeI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MslI MunI NdeI NheI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI PvuII RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 1120 GACGTC GACGT^C AclI 1039 AACGTT AA^CGTT AflII 403 CTTAAG C^TTAAG AflIII 626 ACACGT A^C[GA][CT]GT AhdI 61 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1318 GTGCAC G^TGCAC AlwNI 1406 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1049 CCTCAGG CC^T[ACGT]AGG Aor51HI 1303 AGCGCT AGC^GCT ApaLI 1318 GTGCAC G^TGCAC AspEI 61 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 249 CCCGGG C^[CT]CG[GA]G BbvCI 7 GCTGAGG CCTCAGC(-5/-2) BfaI 853 CTAG C^TAG BfrI 403 CTTAAG C^TTAAG BsaAI 904 TACGTG [CT]AC^GT[GA] BseRI 22 GAGGAG GAGGAG(10/8) BsiEI 261 CGGCCG CG[GA][CT]^CG BsmBI 601 GAGACG CGTCTC(1/5) BsoBI 249 CCCGGG C^[CT]CG[GA]G BsrFI 227 GCCGGC [GA]^CCGG[CT] BssSI 421 CTCGTG CACGAG(-5/-1) BstYI 749 AGATCC [GA]^GATC[CT] Bsu36I 1049 CCTCAGG CC^T[ACGT]AGG Cfr10I 227 GCCGGC [GA]^CCGG[CT] EagI 261 CGGCCG C^GGCCG Eam1105I 61 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 261 CGGCCG C^GGCCG Eco47III 1303 AGCGCT AGC^GCT Eco52I 261 CGGCCG C^GGCCG Eco81I 1049 CCTCAGG CC^T[ACGT]AGG EcoT14I 356 CCATGG C^C[AT][AT]GG EcoT22I 445 ATGCAT ATGCA^T FauI 172 CCCGC CCCGC(4/6) HgaI 219 GACGC GACGC(5/10) KspI 96 CCGCGG CCGC^GG MaeI 853 CTAG C^TAG MaeIII 1408 GTGAC ^GT[ACGT]AC MflI 749 AGATCC [GA]^GATC[CT] NaeI 227 GCCGGC GCC^GGC NcoI 356 CCATGG C^CATGG NgoMIV 227 GCCGGC G^CCGGC NotI 260 GCGGCCGC GC^GGCCGC NsiI 445 ATGCAT ATGCA^T Psp1406I 1039 AACGTT AA^CGTT SacII 96 CCGCGG CCGC^GG SfcI 1299 CTACAG C^T[GA][CT]AG SfiI 253 GGCCTGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 226 CGCCGGCG C[GA]^CCGG[CT]G SmaI 249 CCCGGG CCC^GGG SmlI 403 CTTAAG C^T[CT][GA]AG StuI 374 AGGCCT AGG^CCT StyI 356 CCATGG C^C[AT][AT]GG Tsp45I 1408 GTGAC ^GT[GC]AC Tsp509I 1131 AATT ^AATT XcmI 188 CCAACTGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 749 AGATCC [GA]^GATC[CT] XmaCI 249 CCCGGG C^CCGGG XmaI 249 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 97 CGCG CG^CG 177 CGCG AlwI 750 GATCC GGATC(4/5) 1272 GGATC ApaI 194 GGGCCC GGGCC^C 237 GGGCCC AspHI 340 GAGCAC G[AT]GC[AT]^C 1318 GTGCAC AvaII 60 GGACC G^G[AT]CC 564 GGACC BanII 194 GGGCCC G[GA]GC[CT]^C 237 GGGCCC BglI 254 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 276 GCCGCTGCGGC BpmI 706 CTGGAG CTGGAG(16/14) 1090 CTGGAG BsiHKAI 340 GAGCAC G[AT]GC[AT]^C 1318 GTGCAC BspMI 31 ACCTGC ACCTGC(4/8) 1322 ACCTGC BstUI 97 CGCG CG^CG 177 CGCG BstXI 38 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1363 CCATTTTTTTGG DraIII 477 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 1252 CACCGAGTG DsaI 96 CCGCGG C^C[GA][CT]GG 356 CCATGG HincII 27 GTTGAC GT[CT]^[GA]AC 735 GTTGAC HindII 27 GTTGAC GT[CT]^[GA]AC 735 GTTGAC HphI 786 GGTGA GGTGA(8/7) 1046 TCACC MseI 404 TTAA T^TAA 631 TTAA MvnI 97 CGCG CG^CG 177 CGCG PpuMI 59 AGGACCC [GA]G^G[AT]CC[CT] 563 AGGACCT SapI 687 GAAGAGC GCTCTTC(1/4) 285 GCTCTTC Tru9I 404 TTAA T^TAA 631 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcyI AluI BalI BanI BbeI BbsI BbvI BcnI BmyI BpuAI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI Ksp632I MaeII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PleI Sau3AI Sau96I ScrFI SfaNI SfoI TaqI TfiI TseI TspRI TthHB8I Van91I