Restriction map for pFN21AE2021
 (Linear) (Six-base) MAPSORT of: KIEE2021.seq  Check: 4346  from: 1  to: 1797



KIEE2021.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:07  ..

AccI GT'mk_AC

Cuts at:      0     298    1797 
   Size:        298    1499
 
AclI AA'CG_TT

Cuts at:      0    1295    1797 
   Size:       1295     502
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     828    1797 
   Size:        545     283     969

  Fragments arranged by size: 

                 969     545     283
 
AfeI AGC'GCT

Cuts at:      0     326    1797 
   Size:        326    1471
 
AflII C'TTAA_G

Cuts at:      0    1132    1797 
   Size:       1132     665
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1629    1797 
   Size:       1629     168
 
AleI CACnn'nnGTG

Cuts at:      0     743    1797 
   Size:        743    1054
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1768    1797 
   Size:       1768      29
 
ApaI G_GGCC'C

Cuts at:      0    1074    1797 
   Size:       1074     723
 
ApaLI G'TGCA_C

Cuts at:      0     736    1797 
   Size:        736    1061
 
ApoI r'AATT_y

Cuts at:      0     363    1797 
   Size:        363    1434
 
AvaI C'yCGr_G

Cuts at:      0    1067    1797 
   Size:       1067     730
 
AvrII C'CTAG_G

Cuts at:      0    1542    1797 
   Size:       1542     255
 
BamHI G'GATC_C

Cuts at:      0      41     482     900    1538    1797 
   Size:         41     441     418     638     259

  Fragments arranged by size: 

                 638     441     418     259      41
 
BanII G_rGCy'C

Cuts at:      0    1074    1797 
   Size:       1074     723
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      23     385    1364    1797 
   Size:         23     362     979     433

  Fragments arranged by size: 

                 979     433     362      23
 
BbvCI CC'TCA_GC

Cuts at:      0     166     586    1495    1797 
   Size:        166     420     909     302

  Fragments arranged by size: 

                 909     420     302     166
 
BclI T'GATC_A

Cuts at:      0    1005    1797 
   Size:       1005     792
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1765    1797 
   Size:       1765      32
 
BglII A'GATC_T

Cuts at:      0     834    1797 
   Size:        834     963
 
Bme1580I G_kGCm'C

Cuts at:      0     598     740    1074    1797 
   Size:        598     142     334     723

  Fragments arranged by size: 

                 723     598     334     142
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1357    1755    1797 
   Size:       1357     398      42

  Fragments arranged by size: 

                1357     398      42
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     228    1797 
   Size:        228    1569
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     872    1756    1797 
   Size:        872     884      41

  Fragments arranged by size: 

                 884     872      41
 
Bpu10I CC'TnA_GC

Cuts at:      0     166     586    1495    1797 
   Size:        166     420     909     302

  Fragments arranged by size: 

                 909     420     302     166
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     738     859    1797 
   Size:        738     121     938

  Fragments arranged by size: 

                 938     738     121
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     342    1797 
   Size:        342    1455
 
BsaAI yAC'GTr

Cuts at:      0     120    1797 
   Size:        120    1677
 
BsaBI GATnn'nnATC

Cuts at:      0     905    1797 
   Size:        905     892
 
BsaHI Gr'CG_yC

Cuts at:      0      14    1797 
   Size:         14    1783
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1240    1270    1768    1797 
   Size:       1240      30     498      29

  Fragments arranged by size: 

                1240     498      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     154    1678    1705    1797 
   Size:        154    1524      27      92

  Fragments arranged by size: 

                1524     154      92      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130    1797 
   Size:        130    1667
 
BsiEI CG_ry'CG

Cuts at:      0     137    1797 
   Size:        137    1660
 
BsiHKAI G_wGCw'C

Cuts at:      0     740    1797 
   Size:        740    1057
 
BsmI GAATG_Cn'

Cuts at:      0     288    1797 
   Size:        288    1509
 
Bsp1286I G_dGCh'C

Cuts at:      0     598     740    1074    1797 
   Size:        598     142     334     723

  Fragments arranged by size: 

                 723     598     334     142
 
BspHI T'CATG_A

Cuts at:      0     264    1797 
   Size:        264    1533
 
BsrDI GCAATG_nn'

Cuts at:      0     464    1283    1589    1797 
   Size:        464     819     306     208

  Fragments arranged by size: 

                 819     464     306     208
 
BsrFI r'CCGG_y

Cuts at:      0      50    1797 
   Size:         50    1747
 
BsrGI T'GTAC_A

Cuts at:      0     533    1797 
   Size:        533    1264
 
BssSI C'ACGA_G

Cuts at:      0     947    1797 
   Size:        947     850
 
BstYI r'GATC_y

Cuts at:      0      41     482     834     900    1538    1797 
   Size:         41     441     352      66     638     259

  Fragments arranged by size: 

                 638     441     352     259      66      41
 
BstZ17I GTA'TAC

Cuts at:      0     299    1797 
   Size:        299    1498
 
Bsu36I CC'TnA_GG

Cuts at:      0      37    1797 
   Size:         37    1760
 
BtgI C'CryG_G

Cuts at:      0      83     137     302    1021    1673    1797 
   Size:         83      54     165     719     652     124

  Fragments arranged by size: 

                 719     652     165     124      83      54
 
BtsI GCAGTG_nn'

Cuts at:      0     598    1786    1797 
   Size:        598    1188      11

  Fragments arranged by size: 

                1188     598      11
 
EaeI y'GGCC_r

Cuts at:      0     134    1797 
   Size:        134    1663
 
EagI C'GGCC_G

Cuts at:      0     134    1797 
   Size:        134    1663
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     828     872    1756    1797 
   Size:        545     283      44     884      41

  Fragments arranged by size: 

                 884     545     283      44      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1071    1731    1797 
   Size:       1071     660      66

  Fragments arranged by size: 

                1071     660      66
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     353    1797 
   Size:        353    1444
 
HaeII r_GCGC'y

Cuts at:      0     328     558    1797 
   Size:        328     230    1239

  Fragments arranged by size: 

                1239     328     230
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     643     675     691     723     775     807     885     917 
   Size:        643      32      16      32      52      32      78      32

Cuts at:    917    1615    1647    1797 
   Size:        698      32     150

  Fragments arranged by size: 

                 698     643     150      78      52      32      32      32
                  32      32      16
 
HindIII A'AGCT_T

Cuts at:      0     825    1797 
   Size:        825     972
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     878    1283    1797 
   Size:        176     702     405     514

  Fragments arranged by size: 

                 702     514     405     176
 
MslI CAynn'nnrTG

Cuts at:      0     667     743    1797 
   Size:        667      76    1054

  Fragments arranged by size: 

                1054     667      76
 
MspA1I CmG'CkG

Cuts at:      0      78      85     139    1101    1797 
   Size:         78       7      54     962     696

  Fragments arranged by size: 

                 962     696      78      54       7
 
NcoI C'CATG_G

Cuts at:      0     302    1797 
   Size:        302    1495
 
NotI GC'GGCC_GC

Cuts at:      0     134    1797 
   Size:        134    1663
 
NspI r_CATG'y

Cuts at:      0    1015    1794    1797 
   Size:       1015     779       3

  Fragments arranged by size: 

                1015     779       3
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     237    1797 
   Size:        237    1560
 
PmlI CAC'GTG

Cuts at:      0     120    1797 
   Size:        120    1677
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1768    1797 
   Size:       1768      29
 
PpuMI rG'GwC_Cy

Cuts at:      0    1731    1797 
   Size:       1731      66
 
PspOMI G'GGCC_C

Cuts at:      0    1070    1797 
   Size:       1070     727
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     759     791    1797 
   Size:        759      32    1006

  Fragments arranged by size: 

                1006     759      32
 
PvuII CAG'CTG

Cuts at:      0    1101    1797 
   Size:       1101     696
 
SacII CC_GC'GG

Cuts at:      0      86     140    1797 
   Size:         86      54    1657

  Fragments arranged by size: 

                1657      86      54
 
SfcI C'TryA_G

Cuts at:      0    1050    1797 
   Size:       1050     747
 
SmaI CCC'GGG

Cuts at:      0    1069    1797 
   Size:       1069     728
 
SmlI C'TyrA_G

Cuts at:      0     753     874    1132    1797 
   Size:        753     121     258     665

  Fragments arranged by size: 

                 753     665     258     121
 
SphI G_CATG'C

Cuts at:      0    1015    1794    1797 
   Size:       1015     779       3

  Fragments arranged by size: 

                1015     779       3
 
StyI C'CwwG_G

Cuts at:      0     302     978    1088    1542    1797 
   Size:        302     676     110     454     255

  Fragments arranged by size: 

                 676     454     302     255     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1014    1797 
   Size:       1014     783
 
TatI w'GTAC_w

Cuts at:      0     533     839    1797 
   Size:        533     306     958

  Fragments arranged by size: 

                 958     533     306
 
Tth111I GACn'n_nGTC

Cuts at:      0    1525    1797 
   Size:       1525     272
 
XbaI T'CTAG_A

Cuts at:      0    1635    1797 
   Size:       1635     162
 
XmaI C'CCGG_G

Cuts at:      0    1067    1797 
   Size:       1067     730
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI    AflII     AhdI     AleI     AloI
     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI    BanII     BbsI
    BbvCI     BclI     BglI    BglII Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrFI
    BsrGI    BssSI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     EaeI
     EagI  Eco57MI EcoO109I     FalI    HaeII    Hin4I  HindIII     MmeI
     MslI   MspA1I     NcoI     NotI     NspI    PflMI     PmlI     PpiI
    PpuMI   PspOMI     PsrI    PvuII    SacII     SfcI     SmaI     SmlI
     SphI     StyI    TaqII     TatI  Tth111I     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I   AflIII     AgeI     AlfI    AlwNI     AscI
     AseI    AsiSI     BaeI     BanI     BbeI     BcgI    BciVI    BfrBI
     BlpI    BmgBI     BmtI    BsaWI    BseYI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI   BssHII   BstAPI    BstBI   BstEII    BstXI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI    PshAI     PsiI    PspXI     PstI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TstI     XcmI     XhoI     XmnI     ZraI
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