(Linear) (Six-base) MAPSORT of: KIEE2044.seq Check: 7779 from: 1 to: 1986 KIEE2044.orf With 182 enzymes: SgfI * July 3, 2012 17:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1153 1545 1986 Size: 1153 392 441 Fragments arranged by size: 1153 441 392 AccI GT'mk_AC Cuts at: 0 1291 1986 Size: 1291 695 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1593 1986 Size: 1593 393 AfeI AGC'GCT Cuts at: 0 1606 1986 Size: 1606 380 AhdI GACnn_n'nnGTC Cuts at: 0 1383 1986 Size: 1383 603 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 80 1019 1636 1986 Size: 80 939 617 350 Fragments arranged by size: 939 617 350 80 AlwNI CAG_nnn'CTG Cuts at: 0 1336 1986 Size: 1336 650 ApaI G_GGCC'C Cuts at: 0 139 1986 Size: 139 1847 ApoI r'AATT_y Cuts at: 0 619 1986 Size: 619 1367 AvaI C'yCGr_G Cuts at: 0 132 927 1079 1270 1894 1986 Size: 132 795 152 191 624 92 Fragments arranged by size: 795 624 191 152 132 92 AvrII C'CTAG_G Cuts at: 0 139 1986 Size: 139 1847 BamHI G'GATC_C Cuts at: 0 1173 1986 Size: 1173 813 BanI G'GyrC_C Cuts at: 0 518 822 1398 1724 1918 1986 Size: 518 304 576 326 194 68 Fragments arranged by size: 576 518 326 304 194 68 BanII G_rGCy'C Cuts at: 0 139 1086 1986 Size: 139 947 900 Fragments arranged by size: 947 900 139 BbeI G_GCGC'C Cuts at: 0 826 1986 Size: 826 1160 BbsI GAAGACnn'nnnn_ Cuts at: 0 1469 1906 1986 Size: 1469 437 80 Fragments arranged by size: 1469 437 80 BbvCI CC'TCA_GC Cuts at: 0 503 1329 1579 1986 Size: 503 826 250 407 Fragments arranged by size: 826 503 407 250 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1662 1696 1986 Size: 1662 34 290 Fragments arranged by size: 1662 290 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1932 1986 Size: 1932 54 BlpI GC'TnA_GC Cuts at: 0 276 1411 1986 Size: 276 1135 575 Fragments arranged by size: 1135 575 276 Bme1580I G_kGCm'C Cuts at: 0 139 1401 1735 1923 1986 Size: 139 1262 334 188 63 Fragments arranged by size: 1262 334 188 139 63 BmgBI CAC'GTC Cuts at: 0 992 1353 1986 Size: 992 361 633 Fragments arranged by size: 992 633 361 BmrI ACTGGGnnnn_n' Cuts at: 0 1404 1804 1986 Size: 1404 400 182 Fragments arranged by size: 1404 400 182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 684 814 1221 1611 1799 1986 Size: 619 65 130 407 390 188 187 Fragments arranged by size: 619 407 390 188 187 130 65 Bpu10I CC'TnA_GC Cuts at: 0 241 503 1329 1579 1669 1925 1986 Size: 241 262 826 250 90 256 61 Fragments arranged by size: 826 262 256 250 241 90 61 BsaI GGTCTCn'nnnn_ Cuts at: 0 472 1498 1986 Size: 472 1026 488 Fragments arranged by size: 1026 488 472 BsaHI Gr'CG_yC Cuts at: 0 823 1427 1688 1986 Size: 823 604 261 298 Fragments arranged by size: 823 604 298 261 BsaWI w'CCGG_w Cuts at: 0 108 521 1986 Size: 108 413 1465 Fragments arranged by size: 1465 413 108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 169 199 839 869 1986 Size: 169 30 640 30 1117 Fragments arranged by size: 1117 640 169 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 22 413 771 948 1986 Size: 22 391 358 177 1038 Fragments arranged by size: 1038 391 358 177 22 BseYI C'CCAG_C Cuts at: 0 211 307 529 642 741 788 1336 1601 Size: 211 96 222 113 99 47 548 265 Cuts at: 1601 1986 Size: 385 Fragments arranged by size: 548 385 265 222 211 113 99 96 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 886 1513 1573 1642 1986 Size: 529 357 627 60 69 344 Fragments arranged by size: 627 529 357 344 69 60 BsiHKAI G_wGCw'C Cuts at: 0 187 1417 1986 Size: 187 1230 569 Fragments arranged by size: 1230 569 187 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1359 1986 Size: 1359 627 Bsp1286I G_dGCh'C Cuts at: 0 139 187 1086 1401 1417 1735 1923 1986 Size: 139 48 899 315 16 318 188 63 Fragments arranged by size: 899 318 315 188 139 63 48 16 BspEI T'CCGG_A Cuts at: 0 108 1986 Size: 108 1878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1153 1545 1614 1986 Size: 1153 392 69 372 Fragments arranged by size: 1153 392 372 69 BsrDI GCAATG_nn' Cuts at: 0 1226 1329 1986 Size: 1226 103 657 Fragments arranged by size: 1226 657 103 BsrFI r'CCGG_y Cuts at: 0 271 1986 Size: 271 1715 BsrGI T'GTAC_A Cuts at: 0 323 1986 Size: 323 1663 BssSI C'ACGA_G Cuts at: 0 1681 1986 Size: 1681 305 BstAPI GCAn_nnn'nTGC Cuts at: 0 1491 1986 Size: 1491 495 BstEII G'GTnAC_C Cuts at: 0 12 384 1986 Size: 12 372 1602 Fragments arranged by size: 1602 372 12 BstYI r'GATC_y Cuts at: 0 1173 1986 Size: 1173 813 Bsu36I CC'TnA_GG Cuts at: 0 380 563 716 1913 1986 Size: 380 183 153 1197 73 Fragments arranged by size: 1197 380 183 153 73 BtgI C'CryG_G Cuts at: 0 538 1986 Size: 538 1448 BtsI GCAGTG_nn' Cuts at: 0 1804 1986 Size: 1804 182 DraIII CAC_nnn'GTG Cuts at: 0 1286 1574 1753 1986 Size: 1286 288 179 233 Fragments arranged by size: 1286 288 233 179 DrdI GACnn_nn'nnGTC Cuts at: 0 372 1986 Size: 372 1614 EaeI y'GGCC_r Cuts at: 0 452 1072 1986 Size: 452 620 914 Fragments arranged by size: 914 620 452 EarI CTCTTCn'nnn_ Cuts at: 0 40 46 1820 1986 Size: 40 6 1774 166 Fragments arranged by size: 1774 166 40 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1752 1986 Size: 1752 234 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 684 814 1221 1593 1611 1799 1986 Size: 619 65 130 407 372 18 188 187 Fragments arranged by size: 619 407 372 188 187 130 65 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 354 1840 1986 Size: 354 1486 146 Fragments arranged by size: 1486 354 146 EcoO109I rG'GnC_Cy Cuts at: 0 96 136 144 202 896 1134 1845 1986 Size: 96 40 8 58 694 238 711 141 Fragments arranged by size: 711 694 238 141 96 58 40 8 EcoRI G'AATT_C Cuts at: 0 619 1986 Size: 619 1367 EcoRV GAT'ATC Cuts at: 0 164 1986 Size: 164 1822 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1299 1986 Size: 1299 687 FspI TGC'GCA Cuts at: 0 1265 1986 Size: 1265 721 HaeII r_GCGC'y Cuts at: 0 65 255 826 1608 1986 Size: 65 190 571 782 378 Fragments arranged by size: 782 571 378 190 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 103 109 135 141 173 565 597 839 Size: 103 6 26 6 32 392 32 242 Cuts at: 839 871 906 938 1369 1401 1404 1436 1875 Size: 32 35 32 431 32 3 32 439 Cuts at: 1875 1907 1986 Size: 32 79 Fragments arranged by size: 439 431 392 242 103 79 35 32 32 32 32 32 32 32 26 6 6 3 KasI G'GCGC_C Cuts at: 0 822 1986 Size: 822 1164 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 323 654 1026 1986 Size: 323 331 372 960 Fragments arranged by size: 960 372 331 323 MscI TGG'CCA Cuts at: 0 1074 1986 Size: 1074 912 MslI CAynn'nnrTG Cuts at: 0 439 1618 1986 Size: 439 1179 368 Fragments arranged by size: 1179 439 368 MspA1I CmG'CkG Cuts at: 0 9 211 462 642 741 792 1199 1336 Size: 9 202 251 180 99 51 407 137 Cuts at: 1336 1451 1740 1986 Size: 115 289 246 Fragments arranged by size: 407 289 251 246 202 180 137 115 99 51 9 NaeI GCC'GGC Cuts at: 0 273 1986 Size: 273 1713 NarI GG'CG_CC Cuts at: 0 823 1986 Size: 823 1163 NcoI C'CATG_G Cuts at: 0 538 1986 Size: 538 1448 NgoMIV G'CCGG_C Cuts at: 0 271 1986 Size: 271 1715 NspI r_CATG'y Cuts at: 0 864 1986 Size: 864 1122 PasI CC'CwG_GG Cuts at: 0 1656 1986 Size: 1656 330 PflMI CCAn_nnn'nTGG Cuts at: 0 1070 1986 Size: 1070 916 PfoI T'CCnGG_A Cuts at: 0 661 1820 1986 Size: 661 1159 166 Fragments arranged by size: 1159 661 166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 496 528 1986 Size: 496 32 1458 Fragments arranged by size: 1458 496 32 PpuMI rG'GwC_Cy Cuts at: 0 96 144 202 896 1986 Size: 96 48 58 694 1090 Fragments arranged by size: 1090 694 96 58 48 PspOMI G'GGCC_C Cuts at: 0 135 1986 Size: 135 1851 PstI C_TGCA'G Cuts at: 0 73 293 395 1150 1841 1986 Size: 73 220 102 755 691 145 Fragments arranged by size: 755 691 220 145 102 73 PvuII CAG'CTG Cuts at: 0 211 462 642 741 792 1199 1336 1986 Size: 211 251 180 99 51 407 137 650 Fragments arranged by size: 650 407 251 211 180 137 99 51 SanDI GG'GwC_CC Cuts at: 0 202 1986 Size: 202 1784 SbfI CC_TGCA'GG Cuts at: 0 1841 1986 Size: 1841 145 SexAI A'CCwGG_T Cuts at: 0 491 1986 Size: 491 1495 SfcI C'TryA_G Cuts at: 0 69 289 391 850 1146 1194 1837 1986 Size: 69 220 102 459 296 48 643 149 Fragments arranged by size: 643 459 296 220 149 102 69 48 SfoI GGC'GCC Cuts at: 0 824 1986 Size: 824 1162 SmaI CCC'GGG Cuts at: 0 1081 1986 Size: 1081 905 SmlI C'TyrA_G Cuts at: 0 1894 1986 Size: 1894 92 SrfI GCCC'GGGC Cuts at: 0 1081 1986 Size: 1081 905 StyI C'CwwG_G Cuts at: 0 139 538 651 884 1692 1701 1952 1986 Size: 139 399 113 233 808 9 251 34 Fragments arranged by size: 808 399 251 233 139 113 34 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 9 330 543 1027 1986 Size: 9 321 213 484 959 Fragments arranged by size: 959 484 321 213 9 TatI w'GTAC_w Cuts at: 0 323 1986 Size: 323 1663 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 153 185 850 882 1986 Size: 153 32 665 32 1104 Fragments arranged by size: 1104 665 153 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 973 1986 Size: 973 1013 XhoI C'TCGA_G Cuts at: 0 1894 1986 Size: 1894 92 XmaI C'CCGG_G Cuts at: 0 1079 1986 Size: 1079 907 Enzymes that do cut: AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI SbfI SexAI SfcI SfoI SmaI SmlI SrfI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AleI AloI ApaLI AscI AseI AsiSI BaeI BclI BfrBI BglI BglII BmtI BplI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BspHI BsrBI BssHII BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EcoICRI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI XmnI ZraI