(Circular) (Six-base) MAPSORT of: pFN21AB5986.seq Check: 7262 from: 1 to: 6412 pFN21AB5986 6412 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4222 278 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2718 3200 3755 1937 Size: 781 482 555 4594 Fragments arranged by size: 4594 781 555 482 Acc65I G'GTAC_C Cuts at: 3609 3733 3609 Size: 124 6288 AclI AA'CG_TT Cuts at: 1563 4539 4912 1563 Size: 2976 373 3063 Fragments arranged by size: 3063 2976 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3126 3370 3429 4475 5859 6127 1632 Size: 1494 244 59 1046 1384 268 1917 Fragments arranged by size: 1917 1494 1384 1046 268 244 59 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6215 AflIII A'CryG_T Cuts at: 1203 5311 6288 1203 Size: 4108 977 1327 Fragments arranged by size: 4108 1327 977 AgeI A'CCGG_T Cuts at: 5243 5243 Size: 6412 AhdI GACnn_n'nnGTC Cuts at: 5141 5141 Size: 6412 AleI CACnn'nnGTG Cuts at: 1166 2526 1166 Size: 1360 5052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6412 AlwNI CAG_nnn'CTG Cuts at: 3658 4155 5727 3658 Size: 497 1572 4343 Fragments arranged by size: 4343 1572 497 ApaLI G'TGCA_C Cuts at: 4469 5625 4469 Size: 1156 5256 ApoI r'AATT_y Cuts at: 1493 2302 2339 2645 3515 3721 3947 4099 6208 Size: 809 37 306 870 206 226 152 2109 Cuts at: 6208 1493 Size: 1697 Fragments arranged by size: 2109 1697 870 809 306 226 206 152 37 AseI AT'TA_AT Cuts at: 160 1032 3324 4966 160 Size: 872 2292 1642 1606 Fragments arranged by size: 2292 1642 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 AvaI C'yCGr_G Cuts at: 1870 1943 2634 3707 3737 1870 Size: 73 691 1073 30 4545 Fragments arranged by size: 4545 1073 691 73 30 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2184 2217 2184 Size: 33 6379 BamHI G'GATC_C Cuts at: 2726 3742 4109 2726 Size: 1016 367 5029 Fragments arranged by size: 5029 1016 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3609 3733 5188 618 Size: 325 217 627 1822 124 1455 1842 Fragments arranged by size: 1842 1822 1455 627 325 217 124 BanII G_rGCy'C Cuts at: 1391 2696 3731 1391 Size: 1305 1035 4072 Fragments arranged by size: 4072 1305 1035 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2838 3312 3355 928 Size: 971 939 474 43 3985 Fragments arranged by size: 3985 971 939 474 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4603 4637 1903 Size: 34 2666 34 3678 Fragments arranged by size: 3678 2666 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3186 4304 5514 3186 Size: 1118 1210 4084 Fragments arranged by size: 4084 1210 1118 BclI T'GATC_A Cuts at: 1548 1548 Size: 6412 BfrBI ATG'CAT Cuts at: 2597 2597 Size: 6412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5023 136 Size: 107 122 71 631 605 3351 1525 Fragments arranged by size: 3351 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6407 6407 Size: 6412 BlpI GC'TnA_GC Cuts at: 3829 3829 Size: 6412 Bme1580I G_kGCm'C Cuts at: 1790 2432 4473 5629 1790 Size: 642 2041 1156 2573 Fragments arranged by size: 2573 2041 1156 642 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6412 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2813 4143 5101 Size: 416 53 83 399 24 1396 1330 958 Cuts at: 5101 442 Size: 1753 Fragments arranged by size: 1753 1396 1330 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6412 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2203 3494 5072 1239 Size: 839 125 1291 1578 2579 Fragments arranged by size: 2579 1578 1291 839 125 Bpu10I CC'TnA_GC Cuts at: 2262 2262 Size: 6412 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3488 4537 5402 5700 3488 Size: 1049 865 298 4200 Fragments arranged by size: 4200 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5075 882 Size: 617 3576 2219 Fragments arranged by size: 3576 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3700 4203 493 Size: 711 2496 503 2702 Fragments arranged by size: 2702 2496 711 503 BsaBI GATnn'nnATC Cuts at: 6406 6406 Size: 6412 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4219 4601 5236 275 Size: 53 83 186 936 2686 382 635 1451 Fragments arranged by size: 2686 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4845 5243 5517 5664 6075 4845 Size: 398 274 147 411 5182 Fragments arranged by size: 5182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2409 2439 1178 Size: 30 165 30 1006 30 5151 Fragments arranged by size: 5151 1006 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6412 BseYI C'CCAG_C Cuts at: 2695 3394 5615 6111 2695 Size: 699 2221 496 2996 Fragments arranged by size: 2996 2221 699 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5839 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3862 4623 5651 1540 Size: 275 186 1861 761 1028 2301 Fragments arranged by size: 2301 1861 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4473 4558 5629 4473 Size: 85 1071 5256 Fragments arranged by size: 5256 1071 85 BsmI GAATG_Cn' Cuts at: 3923 4016 3923 Size: 93 6319 BsmBI CGTCTCn'nnnn_ Cuts at: 2406 2406 Size: 6412 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2432 2696 3731 4473 4558 5629 1391 Size: 399 642 264 1035 742 85 1071 2174 Fragments arranged by size: 2174 1071 1035 742 642 399 264 85 BspEI T'CCGG_A Cuts at: 6075 6075 Size: 6412 BspHI T'CATG_A Cuts at: 4301 6031 4301 Size: 1730 4682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3767 844 Size: 2923 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4299 1394 Size: 429 2476 3507 Fragments arranged by size: 3507 2476 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2772 4907 5081 65 Size: 1183 1524 2135 174 1396 Fragments arranged by size: 2135 1524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2756 5056 5243 1700 Size: 110 946 2300 187 2869 Fragments arranged by size: 2869 2300 946 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6412 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6412 BssSI C'ACGA_G Cuts at: 4472 5484 4472 Size: 1012 5400 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6412 BstBI TT'CG_AA Cuts at: 5216 5216 Size: 6412 BstEII G'GTnAC_C Cuts at: 1184 3633 1184 Size: 2449 3963 BstYI r'GATC_y Cuts at: 1972 2726 3742 4109 4494 4511 5963 6049 6061 Size: 754 1016 367 385 17 1452 86 12 Cuts at: 6061 6407 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1016 754 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3240 3240 Size: 6412 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5860 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5673 Fragments arranged by size: 5673 683 56 BtsI GCAGTG_nn' Cuts at: 3928 4722 4742 3928 Size: 794 20 5598 Fragments arranged by size: 5598 794 20 ClaI AT'CG_AT Cuts at: 1554 3509 1554 Size: 1955 4457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2325 2360 529 Size: 35 1761 35 4581 Fragments arranged by size: 4581 1761 35 35 DraI TTT'AAA Cuts at: 2449 3229 3716 2449 Size: 780 487 5145 Fragments arranged by size: 5145 780 487 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5419 809 Size: 507 4103 1802 Fragments arranged by size: 4103 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2758 3859 4748 Size: 54 1425 50 275 18 928 1101 889 Cuts at: 4748 6377 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1101 928 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3859 1537 Size: 275 2047 4090 Fragments arranged by size: 4090 2047 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3405 4342 1353 Size: 2052 937 3423 Fragments arranged by size: 3423 2052 937 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4999 5373 5519 387 Size: 1064 138 3410 374 146 1280 Fragments arranged by size: 3410 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2203 3126 3370 3429 3494 4475 Size: 393 446 125 923 244 59 65 981 Cuts at: 4475 5072 5859 6127 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 981 923 787 597 446 393 268 244 125 65 59 EcoRI G'AATT_C Cuts at: 3721 4099 3721 Size: 378 6034 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2261 2261 Size: 6412 FspI TGC'GCA Cuts at: 4918 4918 Size: 6412 HaeII r_GCGC'y Cuts at: 1678 5559 1678 Size: 3881 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2038 2070 2711 2743 3342 Size: 32 135 32 466 32 641 32 599 Cuts at: 3342 3374 5053 5085 5127 5159 1373 Size: 32 1679 32 42 32 2626 Fragments arranged by size: 2626 1679 641 599 466 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3756 4002 669 Size: 988 281 1101 717 246 3079 Fragments arranged by size: 3079 1101 988 717 281 246 HindIII A'AGCT_T Cuts at: 748 3138 748 Size: 2390 4022 HpaI GTT'AAC Cuts at: 4002 4002 Size: 6412 KpnI G_GTAC'C Cuts at: 3613 3737 3613 Size: 124 6288 MfeI C'AATT_G Cuts at: 2738 4011 2738 Size: 1273 5139 MluI A'CGCG_T Cuts at: 6288 6288 Size: 6412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5526 5710 1305 Size: 4221 184 2007 Fragments arranged by size: 4221 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4590 Fragments arranged by size: 4590 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2526 2552 2885 3050 4370 4729 Size: 648 843 517 26 333 165 1320 359 Cuts at: 4729 4888 518 Size: 159 2042 Fragments arranged by size: 2042 1320 843 648 517 359 333 165 159 26 MspA1I CmG'CkG Cuts at: 1606 3390 3546 3828 4505 5653 5898 1606 Size: 1784 156 282 677 1148 245 2120 Fragments arranged by size: 2120 1784 1148 677 282 245 156 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5860 NdeI CA'TA_TG Cuts at: 387 387 Size: 6412 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6412 NotI GC'GGCC_GC Cuts at: 3859 3859 Size: 6412 NsiI A_TGCA'T Cuts at: 2599 2599 Size: 6412 NspI r_CATG'y Cuts at: 1132 1229 3619 3770 5315 1132 Size: 97 2390 151 1545 2229 Fragments arranged by size: 2390 2229 1545 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6412 PciI A'CATG_T Cuts at: 5311 5311 Size: 6412 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6412 PfoI T'CCnGG_A Cuts at: 2219 2219 Size: 6412 PmeI GTTT'AAAC Cuts at: 3716 3716 Size: 6412 PmlI CAC'GTG Cuts at: 3700 3700 Size: 6412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4481 4513 5994 6026 4481 Size: 32 1481 32 4867 Fragments arranged by size: 4867 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3574 1613 Size: 1961 4451 PsiI TTA'TAA Cuts at: 2370 2922 3982 2370 Size: 552 1060 4800 Fragments arranged by size: 4800 1060 552 PstI C_TGCA'G Cuts at: 830 3764 830 Size: 2934 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6412 PvuII CAG'CTG Cuts at: 3390 3390 Size: 6412 SalI G'TCGA_C Cuts at: 1936 3754 1936 Size: 1818 4594 SapI GCTCTTCn'nnn_ Cuts at: 3405 3405 Size: 6412 SbfI CC_TGCA'GG Cuts at: 3764 3764 Size: 6412 ScaI AGT'ACT Cuts at: 4660 6372 4660 Size: 1712 4700 SfcI C'TryA_G Cuts at: 826 1045 2205 3760 4895 5576 5767 826 Size: 219 1160 1555 1135 681 191 1471 Fragments arranged by size: 1555 1471 1160 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6412 SmaI CCC'GGG Cuts at: 1872 2636 3739 1872 Size: 764 1103 4545 Fragments arranged by size: 4545 1103 764 SmlI C'TyrA_G Cuts at: 820 1017 1943 3467 4516 5417 5679 820 Size: 197 926 1524 1049 901 262 1553 Fragments arranged by size: 1553 1524 1049 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4203 493 Size: 3710 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6412 SphI G_CATG'C Cuts at: 1132 3770 1132 Size: 2638 3774 SspI AAT'ATT Cuts at: 5 52 4336 5 Size: 47 4284 2081 Fragments arranged by size: 4284 2081 47 StuI AGG'CCT Cuts at: 1517 2680 1517 Size: 1163 5249 StyI C'CwwG_G Cuts at: 513 1065 3851 513 Size: 552 2786 3074 Fragments arranged by size: 3074 2786 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 4439 4456 4609 4794 5239 325 Size: 1255 1806 1053 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 1053 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4658 6370 96 Size: 274 80 33 51 1443 2681 1712 138 Fragments arranged by size: 2681 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2409 2441 3242 3274 6372 6404 2409 Size: 32 801 32 3098 32 2417 Fragments arranged by size: 3098 2417 801 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6412 XbaI T'CTAG_A Cuts at: 2656 3748 2656 Size: 1092 5320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6412 XmaI C'CCGG_G Cuts at: 1870 2634 3737 1870 Size: 764 1103 4545 Fragments arranged by size: 4545 1103 764 XmnI GAAnn'nnTTC Cuts at: 4541 4541 Size: 6412 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4220 276 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI