(Linear) (Six-base) MAPSORT of: KIEE2222.seq Check: 9007 from: 1 to: 738 KIEE2222.orf With 182 enzymes: SgfI * July 3, 2012 17:15 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 680 738 Size: 680 58 AgeI A'CCGG_T Cuts at: 0 155 738 Size: 155 583 AlwNI CAG_nnn'CTG Cuts at: 0 88 207 247 738 Size: 88 119 40 491 Fragments arranged by size: 491 119 88 40 ApoI r'AATT_y Cuts at: 0 631 738 Size: 631 107 AvaI C'yCGr_G Cuts at: 0 396 418 738 Size: 396 22 320 Fragments arranged by size: 396 320 22 BanI G'GyrC_C Cuts at: 0 197 465 738 Size: 197 268 273 Fragments arranged by size: 273 268 197 BbeI G_GCGC'C Cuts at: 0 469 738 Size: 469 269 BglII A'GATC_T Cuts at: 0 333 738 Size: 333 405 BlpI GC'TnA_GC Cuts at: 0 89 738 Size: 89 649 Bme1580I G_kGCm'C Cuts at: 0 379 727 738 Size: 379 348 11 Fragments arranged by size: 379 348 11 BmgBI CAC'GTC Cuts at: 0 387 738 Size: 387 351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 276 738 Size: 202 74 462 Fragments arranged by size: 462 202 74 BsaHI Gr'CG_yC Cuts at: 0 466 738 Size: 466 272 BsaWI w'CCGG_w Cuts at: 0 155 738 Size: 155 583 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 630 738 Size: 630 108 BseYI C'CCAG_C Cuts at: 0 301 738 Size: 301 437 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 738 Size: 292 446 BsiHKAI G_wGCw'C Cuts at: 0 95 738 Size: 95 643 Bsp1286I G_dGCh'C Cuts at: 0 95 379 727 738 Size: 95 284 348 11 Fragments arranged by size: 348 284 95 11 BspHI T'CATG_A Cuts at: 0 498 738 Size: 498 240 BsrFI r'CCGG_y Cuts at: 0 155 738 Size: 155 583 BstXI CCAn_nnnn'nTGG Cuts at: 0 58 738 Size: 58 680 BstYI r'GATC_y Cuts at: 0 297 333 738 Size: 297 36 405 Fragments arranged by size: 405 297 36 BtgI C'CryG_G Cuts at: 0 711 738 Size: 711 27 EaeI y'GGCC_r Cuts at: 0 698 738 Size: 698 40 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 276 680 738 Size: 202 74 404 58 Fragments arranged by size: 404 202 74 58 EcoO109I rG'GnC_Cy Cuts at: 0 120 251 489 738 Size: 120 131 238 249 Fragments arranged by size: 249 238 131 120 EcoRI G'AATT_C Cuts at: 0 631 738 Size: 631 107 HaeII r_GCGC'y Cuts at: 0 89 170 469 738 Size: 89 81 299 269 Fragments arranged by size: 299 269 89 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 96 128 289 321 738 Size: 96 32 161 32 417 Fragments arranged by size: 417 161 96 32 32 HincII GTy'rAC Cuts at: 0 282 738 Size: 282 456 KasI G'GCGC_C Cuts at: 0 465 738 Size: 465 273 MscI TGG'CCA Cuts at: 0 700 738 Size: 700 38 NarI GG'CG_CC Cuts at: 0 466 738 Size: 466 272 PfoI T'CCnGG_A Cuts at: 0 179 485 738 Size: 179 306 253 Fragments arranged by size: 306 253 179 PpuMI rG'GwC_Cy Cuts at: 0 120 489 738 Size: 120 369 249 Fragments arranged by size: 369 249 120 PspXI vC'TCGA_Gb Cuts at: 0 418 738 Size: 418 320 SanDI GG'GwC_CC Cuts at: 0 120 738 Size: 120 618 SfoI GGC'GCC Cuts at: 0 467 738 Size: 467 271 SmlI C'TyrA_G Cuts at: 0 418 738 Size: 418 320 TatI w'GTAC_w Cuts at: 0 689 738 Size: 689 49 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 398 430 738 Size: 398 32 308 Fragments arranged by size: 398 308 32 XhoI C'TCGA_G Cuts at: 0 418 738 Size: 418 320 Enzymes that do cut: AcuI AgeI AlwNI ApoI AvaI BanI BbeI BglII BlpI Bme1580I BmgBI BpmI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrFI BstXI BstYI BtgI EaeI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI MscI NarI PfoI PpuMI PspXI SanDI SfoI SmlI TatI TstI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XcmI XmaI XmnI ZraI