Restriction map for
pFN21AE2258
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspDI BspHI
BspLU11I BspMI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
ClaI DraI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeIII HgaI Hin1I
HindIII HinfI HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MscI MseI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TfiI Tru9I
TseI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 340 GTCTAC GT^[AC][GT]AC
AccIII 191 TCCGGA T^CCGGA
AciI 42 CCGC CCGC(-3/-1)
AcsI 200 GAATTC [GA]^AATT[CT]
ApoI 200 GAATTC [GA]^AATT[CT]
AvaII 78 GGACC G^G[AT]CC
BbsI 301 GAAGAC GAAGAC(2/6)
BbvCI 214 GCTGAGG CCTCAGC(-5/-2)
BpuAI 301 GAAGAC GAAGAC(2/6)
BsaI 125 GAGACC GGTCTC(1/5)
BsaWI 191 TCCGGA [AT]^CCGG[AT]
BseAI 191 TCCGGA T^CCGGA
BsmAI 125 GAGAC GTCTC(1/5)
Bsp1407I 11 TGTACA T^GTACA
BspEI 191 TCCGGA T^CCGGA
BsrBI 42 CCGCTC CCGCTC(-3/-3)
BsrDI 318 CATTGC GCAATG(2/0)
BsrFI 87 GCCGGT [GA]^CCGG[CT]
BsrGI 11 TGTACA T^GTACA
BsrI 260 CCAGT ACTGG(1/-1)
BstYI 59 AGATCC [GA]^GATC[CT]
CfoI 39 GCGC GCG^C
Cfr10I 87 GCCGGT [GA]^CCGG[CT]
Cfr13I 78 GGACC G^G[ACGT]CC
CpoI 77 CGGACCG CG^G[AT]CCG
DrdI 331 GACGAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 19 GAAGAG CTCTTC(1/4)
EcoRI 200 GAATTC G^AATTC
Fnu4HI 41 GCCGC GC^[ACGT]GC
HaeII 38 AGCGCC [GA]GCGC^[CT]
HhaI 39 GCGC GCG^C
HinP1I 39 GCGC G^CGC
HincII 238 GTCAAC GT[CT]^[GA]AC
HindII 238 GTCAAC GT[CT]^[GA]AC
HphI 90 GGTGA GGTGA(8/7)
ItaI 41 GCCGC GC^[ACGT]GC
Ksp632I 19 GAAGAG CTCTTC(1/4)
MaeIII 329 GTGAC ^GT[ACGT]AC
MflI 59 AGATCC [GA]^GATC[CT]
MroI 191 TCCGGA T^CCGGA
MspA1I 277 CAGCTG C[AC]G^C[GT]G
NlaIII 295 CATG CATG^
PvuII 277 CAGCTG CAG^CTG
RsrII 77 CGGACCG CG^G[AT]CCG
SapI 19 GAAGAGC GCTCTTC(1/4)
Sau96I 78 GGACC G^G[ACGT]CC
SexAI 146 ACCTGGT A^CC[AT]GGT
SfcI 324 CTACAG C^T[GA][CT]AG
SspBI 11 TGTACA T^GTACA
TaqI 32 TCGA T^CGA
Tsp45I 329 GTGAC ^GT[GC]AC
Tsp509I 201 AATT ^AATT
TthHB8I 32 TCGA T^CGA
XcmI 260 CCAGTGCCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 59 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 12 GTAC GT^AC
282 GTAC
AlwI 60 GATCC GGATC(4/5)
189 GATCC
BanI 84 GGTGCC G^G[CT][GA]CC
150 GGTGCC
BcnI 75 CCCGG CC^[GC]GG
81 CCGGG
BstF5I 25 CATCC GGATG(2/0)
219 GGATG
DdeI 47 CTGAG C^T[ACGT]AG
215 CTGAG
FokI 25 CATCC GGATG(9/13)
219 GGATG
MnlI 49 GAGG CCTC(7/6)
217 GAGG
NciI 75 CCCGG CC^[GC]GG
81 CCGGG
RsaI 12 GTAC GT^AC
282 GTAC
SfaNI 220 GATGC GCATC(5/9)
24 GCATC
TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^
327 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BsiYI BslI BssKI BstNI
DpnII EcoRII HapII HpaII MboI
MboII MspI MvaI MwoI NdeII
NlaIV Sau3AI ScrFI