(Linear) (Six-base) MAPSORT of: KIEE2203.seq Check: 1954 from: 1 to: 2625 KIEE2203.orf With 182 enzymes: SgfI * July 3, 2012 17:13 .. AccI GT'mk_AC Cuts at: 0 1041 2625 Size: 1041 1584 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 955 1147 1276 1522 2170 2432 2625 Size: 142 813 192 129 246 648 262 193 Fragments arranged by size: 813 648 262 246 193 192 142 129 AflIII A'CryG_T Cuts at: 0 1524 2625 Size: 1524 1101 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2132 2625 Size: 2132 493 AlwNI CAG_nnn'CTG Cuts at: 0 984 2625 Size: 984 1641 ApaLI G'TGCA_C Cuts at: 0 585 2625 Size: 585 2040 ApoI r'AATT_y Cuts at: 0 1639 2040 2200 2625 Size: 1639 401 160 425 Fragments arranged by size: 1639 425 401 160 BanI G'GyrC_C Cuts at: 0 1518 2625 Size: 1518 1107 BanII G_rGCy'C Cuts at: 0 36 1404 1611 2625 Size: 36 1368 207 1014 Fragments arranged by size: 1368 1014 207 36 BbsI GAAGACnn'nnnn_ Cuts at: 0 1763 2507 2625 Size: 1763 744 118 Fragments arranged by size: 1763 744 118 BbvCI CC'TCA_GC Cuts at: 0 2132 2625 Size: 2132 493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 980 1014 2625 Size: 980 34 1611 Fragments arranged by size: 1611 980 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2556 2625 Size: 2556 69 Bme1580I G_kGCm'C Cuts at: 0 589 2625 Size: 589 2036 BmgBI CAC'GTC Cuts at: 0 2505 2625 Size: 2505 120 Bpu10I CC'TnA_GC Cuts at: 0 2132 2625 Size: 2132 493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 816 1361 1790 2625 Size: 816 545 429 835 Fragments arranged by size: 835 816 545 429 BsaI GGTCTCn'nnnn_ Cuts at: 0 523 2625 Size: 523 2102 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2475 2505 2625 Size: 2475 30 120 Fragments arranged by size: 2475 120 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1897 2496 2625 Size: 1897 599 129 Fragments arranged by size: 1897 599 129 BseYI C'CCAG_C Cuts at: 0 423 2625 Size: 423 2202 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1300 2625 Size: 1300 1325 BsiEI CG_ry'CG Cuts at: 0 1148 2625 Size: 1148 1477 BsiHKAI G_wGCw'C Cuts at: 0 589 1404 1712 2625 Size: 589 815 308 913 Fragments arranged by size: 913 815 589 308 BsmI GAATG_Cn' Cuts at: 0 714 2625 Size: 714 1911 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1422 2625 Size: 1422 1203 Bsp1286I G_dGCh'C Cuts at: 0 36 589 1404 1611 1712 2625 Size: 36 553 815 207 101 913 Fragments arranged by size: 913 815 553 207 101 36 BspHI T'CATG_A Cuts at: 0 501 828 2625 Size: 501 327 1797 Fragments arranged by size: 1797 501 327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 129 1198 2625 Size: 129 1069 1427 Fragments arranged by size: 1427 1069 129 BsrBI CCG'CTC Cuts at: 0 334 2625 Size: 334 2291 BsrDI GCAATG_nn' Cuts at: 0 409 2625 Size: 409 2216 BsrFI r'CCGG_y Cuts at: 0 1001 2566 2625 Size: 1001 1565 59 Fragments arranged by size: 1565 1001 59 BssSI C'ACGA_G Cuts at: 0 580 2625 Size: 580 2045 BstXI CCAn_nnnn'nTGG Cuts at: 0 303 1134 1569 2625 Size: 303 831 435 1056 Fragments arranged by size: 1056 831 435 303 BstYI r'GATC_y Cuts at: 0 346 537 1801 2388 2625 Size: 346 191 1264 587 237 Fragments arranged by size: 1264 587 346 237 191 Bsu36I CC'TnA_GG Cuts at: 0 1178 2625 Size: 1178 1447 BtgI C'CryG_G Cuts at: 0 1035 2625 Size: 1035 1590 BtsI GCAGTG_nn' Cuts at: 0 2101 2625 Size: 2101 524 DraI TTT'AAA Cuts at: 0 1391 2046 2625 Size: 1391 655 579 Fragments arranged by size: 1391 655 579 DrdI GACnn_nn'nnGTC Cuts at: 0 2516 2625 Size: 2516 109 EaeI y'GGCC_r Cuts at: 0 11 335 1145 2098 2625 Size: 11 324 810 953 527 Fragments arranged by size: 953 810 527 324 11 EagI C'GGCC_G Cuts at: 0 1145 2625 Size: 1145 1480 EarI CTCTTCn'nnn_ Cuts at: 0 937 1772 1823 2029 2186 2271 2309 2473 Size: 937 835 51 206 157 85 38 164 Cuts at: 2473 2585 2625 Size: 112 40 Fragments arranged by size: 937 835 206 164 157 112 85 51 40 38 EcoICRI GAG'CTC Cuts at: 0 1402 2625 Size: 1402 1223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 955 1147 1276 1522 2170 2432 2625 Size: 142 813 192 129 246 648 262 193 Fragments arranged by size: 813 648 262 246 193 192 142 129 EcoO109I rG'GnC_Cy Cuts at: 0 783 2625 Size: 783 1842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 343 2625 Size: 343 2282 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 45 77 915 947 1651 1683 2625 Size: 45 32 838 32 704 32 942 Fragments arranged by size: 942 838 704 45 32 32 32 HincII GTy'rAC Cuts at: 0 1042 2625 Size: 1042 1583 HindIII A'AGCT_T Cuts at: 0 1241 2625 Size: 1241 1384 MluI A'CGCG_T Cuts at: 0 1524 2625 Size: 1524 1101 MscI TGG'CCA Cuts at: 0 2100 2625 Size: 2100 525 MslI CAynn'nnrTG Cuts at: 0 614 2625 Size: 614 2011 MspA1I CmG'CkG Cuts at: 0 514 2625 Size: 514 2111 NaeI GCC'GGC Cuts at: 0 1003 2625 Size: 1003 1622 NcoI C'CATG_G Cuts at: 0 1035 2625 Size: 1035 1590 NgoMIV G'CCGG_C Cuts at: 0 1001 2625 Size: 1001 1624 PpuMI rG'GwC_Cy Cuts at: 0 783 2625 Size: 783 1842 PstI C_TGCA'G Cuts at: 0 893 1101 2625 Size: 893 208 1524 Fragments arranged by size: 1524 893 208 PvuII CAG'CTG Cuts at: 0 514 2625 Size: 514 2111 SacI G_AGCT'C Cuts at: 0 1404 2625 Size: 1404 1221 SalI G'TCGA_C Cuts at: 0 1040 2625 Size: 1040 1585 SapI GCTCTTCn'nnn_ Cuts at: 0 937 2585 2625 Size: 937 1648 40 Fragments arranged by size: 1648 937 40 ScaI AGT'ACT Cuts at: 0 1103 2625 Size: 1103 1522 SexAI A'CCwGG_T Cuts at: 0 591 2625 Size: 591 2034 SfcI C'TryA_G Cuts at: 0 889 1097 2625 Size: 889 208 1528 Fragments arranged by size: 1528 889 208 SmlI C'TyrA_G Cuts at: 0 795 1376 1805 2625 Size: 795 581 429 820 Fragments arranged by size: 820 795 581 429 StyI C'CwwG_G Cuts at: 0 1035 1603 2625 Size: 1035 568 1022 Fragments arranged by size: 1035 1022 568 TatI w'GTAC_w Cuts at: 0 324 1101 2625 Size: 324 777 1524 Fragments arranged by size: 1524 777 324 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2475 2507 2625 Size: 2475 32 118 Fragments arranged by size: 2475 118 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1566 1629 2625 Size: 1566 63 996 Fragments arranged by size: 1566 996 63 XmnI GAAnn'nnTTC Cuts at: 0 2183 2324 2625 Size: 2183 141 301 Fragments arranged by size: 2183 301 141 Enzymes that do cut: AccI AcuI AflIII AlfI AlwNI ApaLI ApoI BanI BanII BbsI BbvCI BcgI BciVI Bme1580I BmgBI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII MluI MscI MslI MspA1I NaeI NcoI NgoMIV PpuMI PstI PvuII SacI SalI SapI ScaI SexAI SfcI SmlI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI BpmI BsaAI BsaBI BsaHI BsaWI BsiWI BspEI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MmeI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI