Restriction map for
pFN21AE2408
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# NO CUT ENZYMES ( 91 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcnI BfrI BglI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
FauI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 2588 GACGTC GACGT^C
AflIII 3194 ACATGT A^C[GA][CT]GT
AocI 1039 CCTCAGG CC^T[ACGT]AGG
AseI 1555 ATTAAT AT^TAAT
AsnI 1555 ATTAAT AT^TAAT
BanI 2291 GGCGCC G^G[CT][GA]CC
BbeI 2291 GGCGCC GGCGC^C
BcgI 5565 CGAAAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3650 TGATCA T^GATCA
BspLU11I 3194 ACATGT A^CATGT
BsrFI 4151 GCCGGT [GA]^CCGG[CT]
Bsu36I 1039 CCTCAGG CC^T[ACGT]AGG
CfoI 2292 GCGC GCG^C
Cfr10I 4151 GCCGGT [GA]^CCGG[CT]
DraI 4305 TTTAAA TTT^AAA
DraIII 4697 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 587 CCATGG C^C[GA][CT]GG
Eco81I 1039 CCTCAGG CC^T[ACGT]AGG
EcoT22I 6242 ATGCAT ATGCA^T
FbaI 3650 TGATCA T^GATCA
HaeII 2291 GGCGCC [GA]GCGC^[CT]
HapII 4152 CCGG C^CGG
HhaI 2292 GCGC GCG^C
HinP1I 2292 GCGC G^CGC
HpaII 4152 CCGG C^CGG
KasI 2291 GGCGCC G^GCGCC
MspI 4152 CCGG C^CGG
NarI 2291 GGCGCC GG^CGCC
NcoI 587 CCATGG C^CATGG
NsiI 6242 ATGCAT ATGCA^T
PaeR7I 5336 CTCGAG C^TCGAG
PshBI 1555 ATTAAT AT^TAAT
PstI 5078 CTGCAG CTGCA^G
SacI 2879 GAGCTC GAGCT^C
SalI 2600 GTCGAC G^TCGAC
SexAI 896 ACCAGGT A^CC[AT]GGT
SfoI 2291 GGCGCC GGC^GCC
SphI 3664 GCATGC GCATG^C
XhoI 5336 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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Alw44I 4485 GTGCAC G^TGCAC
5573 GTGCAC
ApaLI 4485 GTGCAC G^TGCAC
5573 GTGCAC
AvaI 2738 CTCGGG C^[CT]CG[GA]G
5336 CTCGAG
BalI 4131 TGGCCA TGG^CCA
6447 TGGCCA
BanII 2879 GAGCTC G[GA]GC[CT]^C
5683 GGGCTC
BbvCI 26 CCTCAGC CCTCAGC(-5/-2)
2782 GCTGAGG
BciVI 3463 GGATAC GTATCC(6/5)
6354 GTATCC
BglII 4344 AGATCT A^GATCT
5412 AGATCT
BlpI 2881 GCTCAGC GC^T[ACGT]AGC
3200 GCTTAGC
Bpu1102I 2881 GCTCAGC GC^T[ACGT]AGC
3200 GCTTAGC
BsmBI 4103 CGTCTC CGTCTC(1/5)
2586 GAGACG
BsoBI 2738 CTCGGG C^[CT]CG[GA]G
5336 CTCGAG
BspHI 438 TCATGA T^CATGA
6234 TCATGA
BspMI 2142 ACCTGC ACCTGC(4/8)
5076 ACCTGC
BsrBI 3082 GAGCGG CCGCTC(-3/-3)
5641 GAGCGG
BstAPI 2311 GCATATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2463 GCAGGGAATGC
BstEII 17 GGTTACC G^GT[ACGT]ACC
4012 GGTGACC
BstPI 17 GGTTACC G^GT[ACGT]ACC
4012 GGTGACC
CelII 2881 GCTCAGC GC^T[ACGT]AGC
3200 GCTTAGC
DraII 5174 AGGACCT [GA]G^G[ACGT]CC[CT]
6191 AGGACCT
DrdI 4752 GACTCCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5994 GACTAATGAGTC
EaeI 4131 TGGCCA [CT]^GGCC[GA]
6447 TGGCCA
EcoO109I 5174 AGGACCT [GA]G^G[ACGT]CC[CT]
6191 AGGACCT
EcoO65I 17 GGTTACC G^GT[ACGT]ACC
4012 GGTGACC
EcoRI 404 GAATTC G^AATTC
2626 GAATTC
EcoRV 2389 GATATC GAT^ATC
3988 GATATC
MluNI 4131 TGGCCA TGG^CCA
6447 TGGCCA
MscI 4131 TGGCCA TGG^CCA
6447 TGGCCA
PpuMI 5174 AGGACCT [GA]G^G[AT]CC[CT]
6191 AGGACCT
RcaI 438 TCATGA T^CATGA
6234 TCATGA
SfcI 4617 CTACAG C^T[GA][CT]AG
5078 CTGCAG
SspI 3334 AATATT AAT^ATT
4930 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI Hin1I HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmnI