Restriction map for
pFN21AE2408
(Linear) (Six-base) MAPSORT of: KIEE2408.seq Check: 128 from: 1 to: 6504
KIEE2408.orf
With 182 enzymes: SgfI *
June 11, 2012 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2151 6504
Size: 2151 4353
AatII G_ACGT'C
Cuts at: 0 2592 6504
Size: 2592 3912
AccI GT'mk_AC
Cuts at: 0 234 1129 2601 6504
Size: 234 895 1472 3903
Fragments arranged by size:
3903 1472 895 234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1271 1372 1832 2009 2372 2397 3610
Size: 62 1209 101 460 177 363 25 1213
Cuts at: 3610 3617 3788 4363 4843 4876 5051 6062 6143
Size: 7 171 575 480 33 175 1011 81
Cuts at: 6143 6504
Size: 361
Fragments arranged by size:
1213 1209 1011 575 480 460 363 361
177 175 171 101 81 62 33 25
7
AflIII A'CryG_T
Cuts at: 0 3194 6504
Size: 3194 3310
AleI CACnn'nnGTG
Cuts at: 0 1115 4915 6504
Size: 1115 3800 1589
Fragments arranged by size:
3800 1589 1115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4500 6504
Size: 4500 2004
AlwNI CAG_nnn'CTG
Cuts at: 0 2107 3436 5473 6504
Size: 2107 1329 2037 1031
Fragments arranged by size:
2107 2037 1329 1031
ApaLI G'TGCA_C
Cuts at: 0 4485 5573 6504
Size: 4485 1088 931
Fragments arranged by size:
4485 1088 931
ApoI r'AATT_y
Cuts at: 0 262 404 2626 2888 5240 5548 6504
Size: 262 142 2222 262 2352 308 956
Fragments arranged by size:
2352 2222 956 308 262 262 142
AseI AT'TA_AT
Cuts at: 0 1556 6504
Size: 1556 4948
AvaI C'yCGr_G
Cuts at: 0 2738 5336 6504
Size: 2738 2598 1168
Fragments arranged by size:
2738 2598 1168
BanI G'GyrC_C
Cuts at: 0 2291 6504
Size: 2291 4213
BanII G_rGCy'C
Cuts at: 0 2883 5687 6504
Size: 2883 2804 817
Fragments arranged by size:
2883 2804 817
BbeI G_GCGC'C
Cuts at: 0 2295 6504
Size: 2295 4209
BbsI GAAGACnn'nnnn_
Cuts at: 0 2544 2985 3197 3598 3971 5567 6056 6504
Size: 2544 441 212 401 373 1596 489 448
Fragments arranged by size:
2544 1596 489 448 441 401 373 212
BbvCI CC'TCA_GC
Cuts at: 0 27 2783 6504
Size: 27 2756 3721
Fragments arranged by size:
3721 2756 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5554 5588 6504
Size: 5554 34 916
Fragments arranged by size:
5554 916 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3457 6365 6504
Size: 3457 2908 139
Fragments arranged by size:
3457 2908 139
BclI T'GATC_A
Cuts at: 0 3650 6504
Size: 3650 2854
BfrBI ATG'CAT
Cuts at: 0 6244 6504
Size: 6244 260
BglII A'GATC_T
Cuts at: 0 4344 5412 6504
Size: 4344 1068 1092
Fragments arranged by size:
4344 1092 1068
BlpI GC'TnA_GC
Cuts at: 0 2882 3201 6504
Size: 2882 319 3303
Fragments arranged by size:
3303 2882 319
Bme1580I G_kGCm'C
Cuts at: 0 2066 3847 4489 5577 6504
Size: 2066 1781 642 1088 927
Fragments arranged by size:
2066 1781 1088 927 642
BmrI ACTGGGnnnn_n'
Cuts at: 0 217 409 1714 1790 2130 4531 6504
Size: 217 192 1305 76 340 2401 1973
Fragments arranged by size:
2401 1973 1305 340 217 192 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 128 3602 3839 5024 5663 6504
Size: 29 99 3474 237 1185 639 841
Fragments arranged by size:
3474 1185 841 639 237 99 29
Bpu10I CC'TnA_GC
Cuts at: 0 27 249 300 612 2783 5686 5939 6504
Size: 27 222 51 312 2171 2903 253 565
Fragments arranged by size:
2903 2171 565 312 253 222 51 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 2379 2657 3741 5252 5263 6082 6504
Size: 750 1629 278 1084 1511 11 819 422
Fragments arranged by size:
1629 1511 1084 819 750 422 278 11
BsaHI Gr'CG_yC
Cuts at: 0 2292 2589 4149 6504
Size: 2292 297 1560 2355
Fragments arranged by size:
2355 2292 1560 297
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3837 3867 4782 4812 5341 5371 6504
Size: 3837 30 915 30 529 30 1133
Fragments arranged by size:
3837 1133 915 529 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 27 1505 2800 3415 3738 3896 4745 4790
Size: 27 1478 1295 615 323 158 849 45
Cuts at: 4790 6504
Size: 1714
Fragments arranged by size:
1714 1478 1295 849 615 323 158 45
27
BseYI C'CCAG_C
Cuts at: 0 163 426 1025 4794 6504
Size: 163 263 599 3769 1710
Fragments arranged by size:
3769 1710 599 263 163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 3310 5711 6504
Size: 356 2954 2401 793
Fragments arranged by size:
2954 2401 793 356
BsiHKAI G_wGCw'C
Cuts at: 0 2883 4489 5577 6504
Size: 2883 1606 1088 927
Fragments arranged by size:
2883 1606 1088 927
BsmI GAATG_Cn'
Cuts at: 0 255 1309 2474 5744 6504
Size: 255 1054 1165 3270 760
Fragments arranged by size:
3270 1165 1054 760 255
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2580 4109 6504
Size: 2580 1529 2395
Fragments arranged by size:
2580 2395 1529
Bsp1286I G_dGCh'C
Cuts at: 0 2066 2883 3847 4489 5577 5687 6504
Size: 2066 817 964 642 1088 110 817
Fragments arranged by size:
2066 1088 964 817 817 642 110
BspHI T'CATG_A
Cuts at: 0 438 6234 6504
Size: 438 5796 270
Fragments arranged by size:
5796 438 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2151 5085 6504
Size: 2151 2934 1419
Fragments arranged by size:
2934 2151 1419
BsrBI CCG'CTC
Cuts at: 0 3084 5643 6504
Size: 3084 2559 861
Fragments arranged by size:
3084 2559 861
BsrDI GCAATG_nn'
Cuts at: 0 719 1565 2525 2625 4111 4560 5381 5933
Size: 719 846 960 100 1486 449 821 552
Cuts at: 5933 6504
Size: 571
Fragments arranged by size:
1486 960 846 821 719 571 552 449
100
BsrFI r'CCGG_y
Cuts at: 0 4151 6504
Size: 4151 2353
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2317 2469 6504
Size: 2317 152 4035
Fragments arranged by size:
4035 2317 152
BstEII G'GTnAC_C
Cuts at: 0 17 4012 6504
Size: 17 3995 2492
Fragments arranged by size:
3995 2492 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1483 2940 4917 6504
Size: 1483 1457 1977 1587
Fragments arranged by size:
1977 1587 1483 1457
BstYI r'GATC_y
Cuts at: 0 1282 4344 4807 5412 6504
Size: 1282 3062 463 605 1092
Fragments arranged by size:
3062 1282 1092 605 463
Bsu36I CC'TnA_GG
Cuts at: 0 1040 6504
Size: 1040 5464
BtgI C'CryG_G
Cuts at: 0 587 6504
Size: 587 5917
BtsI GCAGTG_nn'
Cuts at: 0 792 2479 5577 5727 6504
Size: 792 1687 3098 150 777
Fragments arranged by size:
3098 1687 792 777 150
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 93 123 128 158 6504
Size: 93 30 5 30 6346
Fragments arranged by size:
6346 93 30 30 5
DraI TTT'AAA
Cuts at: 0 4307 6504
Size: 4307 2197
DraIII CAC_nnn'GTG
Cuts at: 0 4702 6504
Size: 4702 1802
DrdI GACnn_nn'nnGTC
Cuts at: 0 4758 6000 6504
Size: 4758 1242 504
Fragments arranged by size:
4758 1242 504
EaeI y'GGCC_r
Cuts at: 0 4131 6447 6504
Size: 4131 2316 57
Fragments arranged by size:
4131 2316 57
EarI CTCTTCn'nnn_
Cuts at: 0 1339 1826 2205 2486 3821 4028 4862 5587
Size: 1339 487 379 281 1335 207 834 725
Cuts at: 5587 5660 6504
Size: 73 844
Fragments arranged by size:
1339 1335 844 834 725 487 379 281
207 73
EcoICRI GAG'CTC
Cuts at: 0 2881 6504
Size: 2881 3623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 62 128 1271 1372 1832 2009 2372
Size: 29 33 66 1143 101 460 177 363
Cuts at: 2372 2397 3602 3610 3617 3788 3839 4363 4843
Size: 25 1205 8 7 171 51 524 480
Cuts at: 4843 4876 5024 5051 5663 6062 6143 6504
Size: 33 148 27 612 399 81 361
Fragments arranged by size:
1205 1143 612 524 480 460 399 363
361 177 171 148 101 81 66 51
33 33 29 27 25 8 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1354 4958 5401 6504
Size: 1354 3604 443 1103
Fragments arranged by size:
3604 1354 1103 443
EcoO109I rG'GnC_Cy
Cuts at: 0 5175 6192 6504
Size: 5175 1017 312
Fragments arranged by size:
5175 1017 312
EcoRI G'AATT_C
Cuts at: 0 404 2626 6504
Size: 404 2222 3878
Fragments arranged by size:
3878 2222 404
EcoRV GAT'ATC
Cuts at: 0 2391 3990 6504
Size: 2391 1599 2514
Fragments arranged by size:
2514 2391 1599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 115 3010 3430 3818 5851 6173 6504
Size: 115 2895 420 388 2033 322 331
Fragments arranged by size:
2895 2033 420 388 331 322 115
HaeII r_GCGC'y
Cuts at: 0 2295 6504
Size: 2295 4209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1864 1879 1896 1911 2574 2606 3236 3268
Size: 1864 15 17 15 663 32 630 32
Cuts at: 3268 3697 3729 5341 5373 5461 5493 6037 6043
Size: 429 32 1612 32 88 32 544 6
Cuts at: 6043 6069 6075 6504
Size: 26 6 429
Fragments arranged by size:
1864 1612 663 630 544 429 429 88
32 32 32 32 32 26 17 15
15 6 6
HincII GTy'rAC
Cuts at: 0 678 2602 4538 4736 6504
Size: 678 1924 1936 198 1768
Fragments arranged by size:
1936 1924 1768 678 198
HindIII A'AGCT_T
Cuts at: 0 2704 3599 3629 4411 5183 6504
Size: 2704 895 30 782 772 1321
Fragments arranged by size:
2704 1321 895 782 772 30
KasI G'GCGC_C
Cuts at: 0 2291 6504
Size: 2291 4213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2572 5437 6504
Size: 2572 2865 1067
Fragments arranged by size:
2865 2572 1067
MscI TGG'CCA
Cuts at: 0 4133 6449 6504
Size: 4133 2316 55
Fragments arranged by size:
4133 2316 55
MslI CAynn'nnrTG
Cuts at: 0 1115 3286 4915 6239 6504
Size: 1115 2171 1629 1324 265
Fragments arranged by size:
2171 1629 1324 1115 265
MspA1I CmG'CkG
Cuts at: 0 163 3433 5083 6504
Size: 163 3270 1650 1421
Fragments arranged by size:
3270 1650 1421 163
NarI GG'CG_CC
Cuts at: 0 2292 6504
Size: 2292 4212
NcoI C'CATG_G
Cuts at: 0 587 6504
Size: 587 5917
NsiI A_TGCA'T
Cuts at: 0 6246 6504
Size: 6246 258
NspI r_CATG'y
Cuts at: 0 3198 3668 5581 6504
Size: 3198 470 1913 923
Fragments arranged by size:
3198 1913 923 470
PciI A'CATG_T
Cuts at: 0 3194 6504
Size: 3194 3310
PflMI CCAn_nnn'nTGG
Cuts at: 0 229 663 2191 2614 4471 6504
Size: 229 434 1528 423 1857 2033
Fragments arranged by size:
2033 1857 1528 434 423 229
PfoI T'CCnGG_A
Cuts at: 0 2578 3240 3855 6504
Size: 2578 662 615 2649
Fragments arranged by size:
2649 2578 662 615
PpuMI rG'GwC_Cy
Cuts at: 0 5175 6192 6504
Size: 5175 1017 312
Fragments arranged by size:
5175 1017 312
PsiI TTA'TAA
Cuts at: 0 6290 6504
Size: 6290 214
PstI C_TGCA'G
Cuts at: 0 5082 6504
Size: 5082 1422
PvuII CAG'CTG
Cuts at: 0 163 3433 5083 6504
Size: 163 3270 1650 1421
Fragments arranged by size:
3270 1650 1421 163
SacI G_AGCT'C
Cuts at: 0 2883 6504
Size: 2883 3621
SalI G'TCGA_C
Cuts at: 0 2600 6504
Size: 2600 3904
SapI GCTCTTCn'nnn_
Cuts at: 0 1339 3821 4028 6504
Size: 1339 2482 207 2476
Fragments arranged by size:
2482 2476 1339 207
SexAI A'CCwGG_T
Cuts at: 0 896 6504
Size: 896 5608
SfcI C'TryA_G
Cuts at: 0 4617 5078 6504
Size: 4617 461 1426
Fragments arranged by size:
4617 1426 461
SfoI GGC'GCC
Cuts at: 0 2293 6504
Size: 2293 4211
SmlI C'TyrA_G
Cuts at: 0 765 2394 2672 3720 5267 5278 5336 6097
Size: 765 1629 278 1048 1547 11 58 761
Cuts at: 6097 6504
Size: 407
Fragments arranged by size:
1629 1547 1048 765 761 407 278 58
11
SphI G_CATG'C
Cuts at: 0 3668 6504
Size: 3668 2836
SspI AAT'ATT
Cuts at: 0 3336 4932 6504
Size: 3336 1596 1572
Fragments arranged by size:
3336 1596 1572
StyI C'CwwG_G
Cuts at: 0 587 700 3839 3927 6188 6504
Size: 587 113 3139 88 2261 316
Fragments arranged by size:
3139 2261 587 316 113 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 236 306 1451 2422 3385 3726 3864 4472
Size: 236 70 1145 971 963 341 138 608
Cuts at: 4472 6504
Size: 2032
Fragments arranged by size:
2032 1145 971 963 608 341 236 138
70
TatI w'GTAC_w
Cuts at: 0 561 604 1612 3262 3402 4079 6504
Size: 561 43 1008 1650 140 677 2425
Fragments arranged by size:
2425 1650 1008 677 561 140 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3837 3869 6504
Size: 3837 32 2635
Fragments arranged by size:
3837 2635 32
XbaI T'CTAG_A
Cuts at: 0 801 2892 5415 6378 6504
Size: 801 2091 2523 963 126
Fragments arranged by size:
2523 2091 963 801 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 226 338 5912 6504
Size: 226 112 5574 592
Fragments arranged by size:
5574 592 226 112
XhoI C'TCGA_G
Cuts at: 0 5336 6504
Size: 5336 1168
XmnI GAAnn'nnTTC
Cuts at: 0 1782 4515 5834 6504
Size: 1782 2733 1319 670
Fragments arranged by size:
2733 1782 1319 670
ZraI GAC'GTC
Cuts at: 0 2590 6504
Size: 2590 3914
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NarI
NcoI NsiI NspI PciI PflMI PfoI PpuMI PsiI
PstI PvuII SacI SalI SapI SexAI SfcI SfoI
SmlI SphI SspI StyI TaqII TatI TstI XbaI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI
AscI AsiSI AvrII BaeI BamHI BglI BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI
BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI EagI
EciI FseI FspI FspAI HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SanDI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XmaI