################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BamHI BlnI BsgI BsiEI BsiWI BspDI BspHI BssHII BssSI BstEII BstPI ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoT22I FseI HpaI KpnI KspI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SpeI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 4764 GTATAC GT^[AC][GT]AC AccII 4291 CGCG CG^CG AcyI 4127 GGCGCC G[GA]^CG[CT]C Alw44I 1877 GTGCAC G^TGCAC Aor51HI 5510 AGCGCT AGC^GCT ApaLI 1877 GTGCAC G^TGCAC BbeI 4127 GGCGCC GGCGC^C BbrPI 3353 CACGTG CAC^GTG BcgI 1839 GCAGTTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2471 GGATAC GTATCC(6/5) BglI 1295 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 4127 GGCGCC G[GA]^CG[CT]C BsaI 4651 GAGACC GGTCTC(1/5) BsmBI 5351 GAGACG CGTCTC(1/5) Bsp1407I 5109 TGTACA T^GTACA BsrBI 3373 GAGCGG CCGCTC(-3/-3) BsrFI 4124 ACCGGC [GA]^CCGG[CT] BsrGI 5109 TGTACA T^GTACA Bst1107I 4764 GTATAC GTA^TAC BstAPI 3796 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3617 TTCGAA TT^CGAA BstUI 4291 CGCG CG^CG BstZ17I 4764 GTATAC GTA^TAC Cfr10I 4124 ACCGGC [GA]^CCGG[CT] DraIII 2868 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 5510 AGCGCT AGC^GCT EcoRV 1237 GATATC GAT^ATC Hin1I 4127 GGCGCC G[GA]^CG[CT]C HincII 3096 GTCAAC GT[CT]^[GA]AC HindII 3096 GTCAAC GT[CT]^[GA]AC KasI 4127 GGCGCC G^GCGCC MfeI 2212 CAATTG C^AATTG MunI 2212 CAATTG C^AATTG MvnI 4291 CGCG CG^CG NarI 4127 GGCGCC GG^CGCC NspV 3617 TTCGAA TT^CGAA PaeR7I 4976 CTCGAG C^TCGAG PmaCI 3353 CACGTG CAC^GTG PmlI 3353 CACGTG CAC^GTG PshAI 2980 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2678 CTGCAG CTGCA^G ScaI 1865 AGTACT AGT^ACT SfoI 4127 GGCGCC GGC^GCC SfuI 3617 TTCGAA TT^CGAA SgrAI 4123 CACCGGCG C[GA]^CCGG[CT]G SmaI 486 CCCGGG CCC^GGG SphI 5288 GCATGC GCATG^C SspBI 5109 TGTACA T^GTACA SspI 1759 AATATT AAT^ATT XbaI 1135 TCTAGA T^CTAGA XhoI 4976 CTCGAG C^TCGAG XmaCI 486 CCCGGG C^CCGGG XmaI 486 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 2525 TCCGGA T^CCGGA 3421 TCCGGA AflIII 1664 ACATGT A^C[GA][CT]GT 3317 ACATGT Asp700I 682 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3610 GAATTCATTC AspI 3485 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4149 GACGCTGTC BbvCI 1282 CCTCAGC CCTCAGC(-5/-2) 4930 CCTCAGC BclI 230 TGATCA T^GATCA 2288 TGATCA BglII 4191 AGATCT A^GATCT 4403 AGATCT BsaBI 2023 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5077 GATGGTGATC BsaWI 2525 TCCGGA [AT]^CCGG[AT] 3421 TCCGGA BseAI 2525 TCCGGA T^CCGGA 3421 TCCGGA BspEI 2525 TCCGGA T^CCGGA 3421 TCCGGA BspLU11I 1664 ACATGT A^CATGT 3317 ACATGT BspMI 2076 GCAGGT ACCTGC(4/8) 5049 GCAGGT BsrDI 2814 CATTGC GCAATG(2/0) 5583 CATTGC EcoNI 5133 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5544 CCTGTCAGAGG EcoRI 1616 GAATTC G^AATTC 3610 GAATTC FbaI 230 TGATCA T^GATCA 2288 TGATCA HaeII 4127 GGCGCC [GA]GCGC^[CT] 5510 AGCGCT MamI 2023 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5077 GATGGTGATC MroI 2525 TCCGGA T^CCGGA 3421 TCCGGA NcoI 1690 CCATGG C^CATGG 5370 CCATGG PflFI 3485 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4149 GACGCTGTC SapI 814 GAAGAGC GCTCTTC(1/4) 5503 GAAGAGC SnaBI 3020 TACGTA TAC^GTA 4658 TACGTA Tth111I 3485 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4149 GACGCTGTC XmnI 682 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3610 GAATTCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII AviII BalI BanI BanII BbsI BbvI BcnI BfaI BfrI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII