(Circular) (Six-base) MAPSORT of: pFN21AE3483.seq Check: 6776 from: 1 to: 9943 pFN21AE3483 9943 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6008 6469 6008 Size: 461 9482 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7753 278 Size: 53 83 186 936 6217 2468 Fragments arranged by size: 6217 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7286 1937 Size: 5349 4594 Acc65I G'GTAC_C Cuts at: 7264 7264 Size: 9943 AclI AA'CG_TT Cuts at: 1563 8070 8443 1563 Size: 6507 373 3063 Fragments arranged by size: 6507 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2475 2812 4512 4758 5211 5968 6213 6288 Size: 843 337 1700 246 453 757 245 75 Cuts at: 6288 6339 6869 8006 9390 9658 1632 Size: 51 530 1137 1384 268 1917 Fragments arranged by size: 1917 1700 1384 1137 843 757 530 453 337 268 246 245 75 51 AfeI AGC'GCT Cuts at: 4775 4775 Size: 9943 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9746 AflIII A'CryG_T Cuts at: 1203 2032 8842 9819 1203 Size: 829 6810 977 1327 Fragments arranged by size: 6810 1327 977 829 AgeI A'CCGG_T Cuts at: 8774 8774 Size: 9943 AhdI GACnn_n'nnGTC Cuts at: 5641 5974 8672 5641 Size: 333 2698 6912 Fragments arranged by size: 6912 2698 333 AleI CACnn'nnGTG Cuts at: 1166 2651 1166 Size: 1485 8458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9943 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3663 3695 3663 Size: 32 9911 AlwNI CAG_nnn'CTG Cuts at: 2102 4412 4718 6145 6925 7000 7686 9258 2102 Size: 2310 306 1427 780 75 686 1572 2787 Fragments arranged by size: 2787 2310 1572 1427 780 686 306 75 ApaI G_GGCC'C Cuts at: 3479 5784 7013 3479 Size: 2305 1229 6409 Fragments arranged by size: 6409 2305 1229 ApaLI G'TGCA_C Cuts at: 8000 9156 8000 Size: 1156 8787 ApoI r'AATT_y Cuts at: 1493 2971 3785 7227 7252 7478 7630 9739 1493 Size: 1478 814 3442 25 226 152 2109 1697 Fragments arranged by size: 3442 2109 1697 1478 814 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 8497 160 Size: 872 7465 1606 Fragments arranged by size: 7465 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9943 AvaI C'yCGr_G Cuts at: 1870 1943 3470 5178 6707 6909 7268 1870 Size: 73 1527 1708 1529 202 359 4545 Fragments arranged by size: 4545 1708 1529 1527 359 202 73 BamHI G'GATC_C Cuts at: 5182 6223 6963 7273 7640 5182 Size: 1041 740 310 367 7485 Fragments arranged by size: 7485 1041 740 367 310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 7264 8719 618 Size: 325 217 627 323 5154 1455 1842 Fragments arranged by size: 5154 1842 1455 627 325 323 217 BanII G_rGCy'C Cuts at: 1391 3159 3479 4106 5784 6627 7013 7262 1391 Size: 1768 320 627 1678 843 386 249 4072 Fragments arranged by size: 4072 1768 1678 843 627 386 320 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4052 4149 4960 5286 5928 6279 928 Size: 971 2153 97 811 326 642 351 4592 Fragments arranged by size: 4592 2153 971 811 642 351 326 97 BbvCI CC'TCA_GC Cuts at: 5692 5692 Size: 9943 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8134 8168 1903 Size: 34 6197 34 3678 Fragments arranged by size: 6197 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2476 7835 9045 2476 Size: 5359 1210 3374 Fragments arranged by size: 5359 3374 1210 BclI T'GATC_A Cuts at: 1548 5481 7113 1548 Size: 3933 1632 4378 Fragments arranged by size: 4378 3933 1632 BfrBI ATG'CAT Cuts at: 3905 5072 3905 Size: 1167 8776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4450 4872 8554 Size: 107 122 71 631 605 2778 422 3682 Cuts at: 8554 136 Size: 1525 Fragments arranged by size: 3682 2778 1525 631 605 422 122 107 71 BglII A'GATC_T Cuts at: 6081 7021 9938 6081 Size: 940 2917 6086 Fragments arranged by size: 6086 2917 940 BlpI GC'TnA_GC Cuts at: 2270 7360 2270 Size: 5090 4853 Bme1580I G_kGCm'C Cuts at: 1790 3479 3568 5784 7013 8004 9160 1790 Size: 1689 89 2216 1229 991 1156 2573 Fragments arranged by size: 2573 2216 1689 1229 1156 991 89 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9943 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5593 6754 7674 Size: 416 53 83 399 24 4176 1161 920 Cuts at: 7674 8632 442 Size: 958 1753 Fragments arranged by size: 4176 1753 1161 958 920 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9943 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4233 5524 4233 Size: 1291 8652 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2503 3510 4329 4389 4887 5752 6977 8603 Size: 1264 1007 819 60 498 865 1225 1626 Cuts at: 8603 1239 Size: 2579 Fragments arranged by size: 2579 1626 1264 1225 1007 865 819 498 60 Bpu10I CC'TnA_GC Cuts at: 2394 4770 5692 2394 Size: 2376 922 6645 Fragments arranged by size: 6645 2376 922 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2356 4203 4977 5864 6536 7181 8068 8933 9231 Size: 1847 774 887 672 645 887 865 298 Cuts at: 9231 2356 Size: 3068 Fragments arranged by size: 3068 1847 887 887 865 774 672 645 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2986 3230 5674 8606 882 Size: 617 1487 244 2444 2932 2219 Fragments arranged by size: 2932 2444 2219 1487 617 244 BsaAI yAC'GTr Cuts at: 493 1204 7734 493 Size: 711 6530 2702 Fragments arranged by size: 6530 2702 711 BsaBI GATnn'nnATC Cuts at: 9937 9937 Size: 9943 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6276 6978 7750 8132 Size: 53 83 186 936 4743 702 772 382 Cuts at: 8132 8767 275 Size: 635 1451 Fragments arranged by size: 4743 1451 936 772 702 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8376 8774 9048 9195 9606 8376 Size: 398 274 147 411 8713 Fragments arranged by size: 8713 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2830 2860 3725 3755 4549 Size: 30 165 30 1427 30 865 30 794 Cuts at: 4549 4579 4621 4651 1178 Size: 30 42 30 6470 Fragments arranged by size: 6470 1427 865 794 165 42 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 2756 2759 4332 4835 4887 4890 5113 Size: 1534 36 3 1573 503 52 3 223 Cuts at: 5113 5166 6385 1186 Size: 53 1219 4744 Fragments arranged by size: 4744 1573 1534 1219 503 223 53 52 36 3 3 BseYI C'CCAG_C Cuts at: 3335 4195 5936 6444 6626 9146 9642 3335 Size: 860 1741 508 182 2520 496 3636 Fragments arranged by size: 3636 2520 1741 860 508 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5206 6797 1163 Size: 573 3470 1591 4309 Fragments arranged by size: 4309 3470 1591 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7393 8154 9182 1540 Size: 275 186 5392 761 1028 2301 Fragments arranged by size: 5392 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2648 2751 3468 4430 6522 7180 8004 8089 9160 Size: 103 717 962 2092 658 824 85 1071 Cuts at: 9160 2648 Size: 3431 Fragments arranged by size: 3431 2092 1071 962 824 717 658 103 85 BsmI GAATG_Cn' Cuts at: 2057 3000 5074 7454 7547 2057 Size: 943 2074 2380 93 4453 Fragments arranged by size: 4453 2380 2074 943 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2648 2751 3159 3468 3479 3568 4106 Size: 399 858 103 408 309 11 89 538 Cuts at: 4106 4430 5784 6522 6627 7013 7180 7262 8004 Size: 324 1354 738 105 386 167 82 742 Cuts at: 8004 8089 9160 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1354 1071 858 742 738 538 408 399 386 324 309 167 105 103 89 85 82 11 BspEI T'CCGG_A Cuts at: 9606 9606 Size: 9943 BspHI T'CATG_A Cuts at: 3835 6354 7110 7832 9562 3835 Size: 2519 756 722 1730 4216 Fragments arranged by size: 4216 2519 1730 756 722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4640 6008 6469 6576 6885 7298 844 Size: 3796 1368 461 107 309 413 3489 Fragments arranged by size: 3796 3489 1368 461 413 309 107 BsrBI CCG'CTC Cuts at: 1394 1823 6897 6961 7830 1394 Size: 429 5074 64 869 3507 Fragments arranged by size: 5074 3507 869 429 64 BsrDI GCAATG_nn' Cuts at: 65 1248 2134 8438 8612 65 Size: 1183 886 6304 174 1396 Fragments arranged by size: 6304 1396 1183 886 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2673 5772 8587 8774 1700 Size: 110 863 3099 2815 187 2869 Fragments arranged by size: 3099 2869 2815 863 187 110 BsrGI T'GTAC_A Cuts at: 96 3292 4974 96 Size: 3196 1682 5065 Fragments arranged by size: 5065 3196 1682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9943 BssSI C'ACGA_G Cuts at: 2750 6867 8003 9015 2750 Size: 4117 1136 1012 3678 Fragments arranged by size: 4117 3678 1136 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2061 3254 4760 5069 5220 5441 6023 794 Size: 1267 1193 1506 309 151 221 582 4714 Fragments arranged by size: 4714 1506 1267 1193 582 309 221 151 BstBI TT'CG_AA Cuts at: 8747 8747 Size: 9943 BstEII G'GTnAC_C Cuts at: 1184 3119 4652 1184 Size: 1935 1533 6475 Fragments arranged by size: 6475 1935 1533 BstXI CCAn_nnnn'nTGG Cuts at: 5755 6611 5755 Size: 856 9087 BstYI r'GATC_y Cuts at: 1972 4203 5182 5290 6055 6081 6223 6711 6963 Size: 2231 979 108 765 26 142 488 252 Cuts at: 6963 7021 7273 7640 8025 8042 9494 9580 9592 Size: 58 252 367 385 17 1452 86 12 Cuts at: 9592 9938 1972 Size: 346 1977 Fragments arranged by size: 2231 1977 1452 979 765 488 385 367 346 252 252 142 108 86 58 26 17 12 BtgI C'CryG_G Cuts at: 513 1065 2122 2787 3453 5529 513 Size: 552 1057 665 666 2076 4927 Fragments arranged by size: 4927 2076 1057 666 665 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5389 487 Size: 683 56 4163 5041 Fragments arranged by size: 5041 4163 683 56 BtsI GCAGTG_nn' Cuts at: 4384 5465 5602 6544 7103 7459 8253 8273 4384 Size: 1081 137 942 559 356 794 20 6054 Fragments arranged by size: 6054 1081 942 794 559 356 137 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9943 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9908 DraI TTT'AAA Cuts at: 2068 4789 7247 2068 Size: 2721 2458 4764 Fragments arranged by size: 4764 2721 2458 DraIII CAC_nnn'GTG Cuts at: 6382 6382 Size: 9943 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8950 809 Size: 507 7634 1802 Fragments arranged by size: 7634 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2784 3456 4230 Size: 54 1425 50 275 18 954 672 774 Cuts at: 4230 6927 7390 8279 9908 8 Size: 2697 463 889 1629 43 Fragments arranged by size: 2697 1629 1425 954 889 774 672 463 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7390 1537 Size: 275 5578 4090 Fragments arranged by size: 5578 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2709 2769 3264 3366 4380 5090 6255 6683 Size: 1356 60 495 102 1014 710 1165 428 Cuts at: 6683 7031 7873 1353 Size: 348 842 3423 Fragments arranged by size: 3423 1356 1165 1014 842 710 495 428 348 102 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4790 8530 8904 9050 387 Size: 1064 138 3201 3740 374 146 1280 Fragments arranged by size: 3740 3201 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2475 2503 2812 3510 4329 4389 4512 Size: 393 843 28 309 698 819 60 123 Cuts at: 4512 4758 4887 5211 5752 5968 6213 6288 6339 Size: 246 129 324 541 216 245 75 51 Cuts at: 6339 6869 6977 8006 8603 9390 9658 1239 Size: 530 108 1029 597 787 268 1524 Fragments arranged by size: 1524 1029 843 819 787 698 597 541 530 393 324 309 268 246 245 216 129 123 108 75 60 51 28 EcoNI CCTnn'n_nnAGG Cuts at: 4992 5659 4992 Size: 667 9276 EcoO109I rG'GnC_Cy Cuts at: 3475 3476 5515 5781 7009 7010 3475 Size: 1 2039 266 1228 1 6408 Fragments arranged by size: 6408 2039 1228 266 1 1 EcoRI G'AATT_C Cuts at: 7252 7630 7252 Size: 378 9565 EcoRV GAT'ATC Cuts at: 4782 5572 4782 Size: 790 9153 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2080 3269 4011 6077 2080 Size: 1189 742 2066 5946 Fragments arranged by size: 5946 2066 1189 742 FspI TGC'GCA Cuts at: 5672 8449 5672 Size: 2777 7166 HaeII r_GCGC'y Cuts at: 1678 4777 4915 9090 1678 Size: 3099 138 4175 2531 Fragments arranged by size: 4175 3099 2531 138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3014 3046 5125 5157 5627 Size: 32 135 32 1442 32 2079 32 470 Cuts at: 5627 5659 5960 5992 8584 8616 8658 8690 1373 Size: 32 301 32 2592 32 42 32 2626 Fragments arranged by size: 2626 2592 2079 1442 470 301 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3484 4710 7287 7533 669 Size: 988 281 1546 1226 2577 246 3079 Fragments arranged by size: 3079 2577 1546 1226 988 281 246 HindIII A'AGCT_T Cuts at: 748 2576 4371 4792 748 Size: 1828 1795 421 5899 Fragments arranged by size: 5899 1828 1795 421 HpaI GTT'AAC Cuts at: 3484 7533 3484 Size: 4049 5894 KpnI G_GTAC'C Cuts at: 7268 7268 Size: 9943 MfeI C'AATT_G Cuts at: 7542 7542 Size: 9943 MluI A'CGCG_T Cuts at: 9819 9819 Size: 9943 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4351 4787 5772 9057 9241 1305 Size: 3046 436 985 3285 184 2007 Fragments arranged by size: 3285 3046 2007 985 436 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 3458 4232 6929 10 Size: 54 1425 343 954 672 774 2697 3024 Fragments arranged by size: 3024 2697 1425 954 774 672 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2127 2651 3996 4886 5889 7901 8260 Size: 648 961 524 1345 890 1003 2012 359 Cuts at: 8260 8419 518 Size: 159 2042 Fragments arranged by size: 2042 2012 1345 1003 961 890 648 524 359 159 MspA1I CmG'CkG Cuts at: 1606 4757 5973 6925 6933 7359 8036 9184 9429 Size: 3151 1216 952 8 426 677 1148 245 Cuts at: 9429 1606 Size: 2120 Fragments arranged by size: 3151 2120 1216 1148 952 677 426 245 8 NaeI GCC'GGC Cuts at: 5774 5774 Size: 9943 NcoI C'CATG_G Cuts at: 513 1065 2122 2787 3453 5529 513 Size: 552 1057 665 666 2076 4927 Fragments arranged by size: 4927 2076 1057 666 665 552 NdeI CA'TA_TG Cuts at: 387 3907 6046 387 Size: 3520 2139 4284 Fragments arranged by size: 4284 3520 2139 NgoMIV G'CCGG_C Cuts at: 5772 5772 Size: 9943 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9943 NotI GC'GGCC_GC Cuts at: 7390 7390 Size: 9943 NsiI A_TGCA'T Cuts at: 3907 5074 3907 Size: 1167 8776 NspI r_CATG'y Cuts at: 1132 1229 2036 2630 4891 7301 8846 1132 Size: 97 807 594 2261 2410 1545 2229 Fragments arranged by size: 2410 2261 2229 1545 807 594 97 PasI CC'CwG_GG Cuts at: 1348 6216 1348 Size: 4868 5075 PciI A'CATG_T Cuts at: 2032 8842 2032 Size: 6810 3133 PflMI CCAn_nnn'nTGG Cuts at: 1108 6382 6700 1108 Size: 5274 318 4351 Fragments arranged by size: 5274 4351 318 PfoI T'CCnGG_A Cuts at: 6717 6717 Size: 9943 PmeI GTTT'AAAC Cuts at: 7247 7247 Size: 9943 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8012 8044 9525 9557 8012 Size: 32 1481 32 8398 Fragments arranged by size: 8398 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5515 5515 Size: 9943 PshAI GACnn'nnGTC Cuts at: 1613 6177 1613 Size: 4564 5379 PsiI TTA'TAA Cuts at: 3798 7513 3798 Size: 3715 6228 PspOMI G'GGCC_C Cuts at: 3475 5780 7009 3475 Size: 2305 1229 6409 Fragments arranged by size: 6409 2305 1229 PspXI vC'TCGA_Gb Cuts at: 3470 3470 Size: 9943 PstI C_TGCA'G Cuts at: 830 2910 6622 6921 7236 7295 830 Size: 2080 3712 299 315 59 3478 Fragments arranged by size: 3712 3478 2080 315 299 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9943 PvuII CAG'CTG Cuts at: 4757 5973 6925 6933 4757 Size: 1216 952 8 7767 Fragments arranged by size: 7767 1216 952 8 SalI G'TCGA_C Cuts at: 1936 7285 1936 Size: 5349 4594 SapI GCTCTTCn'nnn_ Cuts at: 2769 3366 4380 6683 2769 Size: 597 1014 2303 6029 Fragments arranged by size: 6029 2303 1014 597 SbfI CC_TGCA'GG Cuts at: 7295 7295 Size: 9943 ScaI AGT'ACT Cuts at: 8191 9903 8191 Size: 1712 8231 SexAI A'CCwGG_T Cuts at: 2044 4703 2044 Size: 2659 7284 SfcI C'TryA_G Cuts at: 826 1045 2214 2906 3324 4174 5078 6618 6917 Size: 219 1169 692 418 850 904 1540 299 Cuts at: 6917 7200 7232 7291 8426 9107 9298 826 Size: 283 32 59 1135 681 191 1471 Fragments arranged by size: 1540 1471 1169 1135 904 850 692 681 418 299 283 219 191 59 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9943 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9943 SmaI CCC'GGG Cuts at: 1872 6911 7270 1872 Size: 5039 359 4545 Fragments arranged by size: 5039 4545 359 SmlI C'TyrA_G Cuts at: 820 1017 1943 2335 3470 4218 4992 5843 6515 Size: 197 926 392 1135 748 774 851 672 Cuts at: 6515 7160 8047 8948 9210 820 Size: 645 887 901 262 1553 Fragments arranged by size: 1553 1135 926 901 887 851 774 748 672 645 392 262 197 SnaBI TAC'GTA Cuts at: 493 7734 493 Size: 7241 2702 SpeI A'CTAG_T Cuts at: 152 3659 152 Size: 3507 6436 SphI G_CATG'C Cuts at: 1132 7301 1132 Size: 6169 3774 SspI AAT'ATT Cuts at: 5 52 7867 5 Size: 47 7815 2081 Fragments arranged by size: 7815 2081 47 StuI AGG'CCT Cuts at: 1517 5665 1517 Size: 4148 5795 StyI C'CwwG_G Cuts at: 513 1065 2122 2787 3453 3686 5382 5511 5529 Size: 552 1057 665 666 233 1696 129 18 Cuts at: 5529 5655 6604 7382 513 Size: 126 949 778 3074 Fragments arranged by size: 3074 1696 1057 949 778 666 665 552 233 129 126 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2128 2135 3736 5452 7970 7987 8140 Size: 1255 548 7 1601 1716 2518 17 153 Cuts at: 8140 8325 8770 325 Size: 185 445 1498 Fragments arranged by size: 2518 1716 1601 1498 1255 548 445 185 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2722 3292 4528 Size: 274 80 33 51 1443 745 570 1236 Cuts at: 4528 4974 7057 8189 9901 96 Size: 446 2083 1132 1712 138 Fragments arranged by size: 2083 1712 1443 1236 1132 745 570 446 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2032 2064 2830 2862 4498 4530 4549 4581 9903 Size: 32 766 32 1636 32 19 32 5322 Cuts at: 9903 9935 2032 Size: 32 2040 Fragments arranged by size: 5322 2040 1636 766 32 32 32 32 32 19 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9943 XbaI T'CTAG_A Cuts at: 5342 7279 5342 Size: 1937 8006 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 5389 5957 6587 2236 Size: 3153 568 630 5592 Fragments arranged by size: 5592 3153 630 568 XhoI C'TCGA_G Cuts at: 1943 3470 1943 Size: 1527 8416 XmaI C'CCGG_G Cuts at: 1870 6909 7268 1870 Size: 5039 359 4545 Fragments arranged by size: 5039 4545 359 XmnI GAAnn'nnTTC Cuts at: 8072 8072 Size: 9943 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7751 276 Size: 53 83 186 936 6217 2468 Fragments arranged by size: 6217 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoICRI FseI FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI