Restriction map for
pFN21AE3826
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# NO CUT ENZYMES ( 46 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
BciVI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI EcoO65I EcoRV EcoT22I FseI
HindIII HpaI MfeI MunI NdeI
NheI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 6079 GACGTC GACGT^C
Acc65I 2189 GGTACC G^GTACC
AcsI 6955 GAATTC [GA]^AATT[CT]
AflII 7600 CTTAAG C^TTAAG
AocI 7315 CCTGAGG CC^T[ACGT]AGG
ApoI 6955 GAATTC [GA]^AATT[CT]
Asp718I 2189 GGTACC G^GTACC
AvrII 7977 CCTAGG C^CTAGG
BamHI 6731 GGATCC G^GATCC
BfaI 7978 CTAG C^TAG
BfrI 7600 CTTAAG C^TTAAG
BlnI 7977 CCTAGG C^CTAGG
BsiWI 1154 CGTACG C^GTACG
BssHII 2319 GCGCGC G^CGCGC
Bsu36I 7315 CCTGAGG CC^T[ACGT]AGG
CpoI 1999 CGGACCG CG^G[AT]CCG
Eco81I 7315 CCTGAGG CC^T[ACGT]AGG
EcoRI 6955 GAATTC G^AATTC
KpnI 2189 GGTACC GGTAC^C
KspI 119 CCGCGG CCGC^GG
MaeI 7978 CTAG C^TAG
MluI 2459 ACGCGT A^CGCGT
NotI 3152 GCGGCCGC GC^GGCCGC
RsrII 1999 CGGACCG CG^G[AT]CCG
SacII 119 CCGCGG CCGC^GG
SbfI 6099 CCTGCAGG CCTGCA^GG
SexAI 1433 ACCTGGT A^CC[AT]GGT
SfiI 1015 GGCCGCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 6099 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 1997 TCCGGA T^CCGGA
4577 TCCGGA
AhdI 4183 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5335 GACAAGAAGTC
AspEI 4183 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5335 GACAAGAAGTC
AviII 1865 TGCGCA TGC^GCA
5057 TGCGCA
BbeI 3424 GGCGCC GGCGC^C
3881 GGCGCC
BbvCI 6305 CCTCAGC CCTCAGC(-5/-2)
949 GCTGAGG
BcgI 4487 CGATTGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1079 GCAATGACATCG
BseAI 1997 TCCGGA T^CCGGA
4577 TCCGGA
BspEI 1997 TCCGGA T^CCGGA
4577 TCCGGA
BspLU11I 1652 ACATGT A^CATGT
7691 ACATGT
EagI 122 CGGCCG C^GGCCG
3153 CGGCCG
Eam1105I 4183 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5335 GACAAGAAGTC
EclXI 122 CGGCCG C^GGCCG
3153 CGGCCG
Eco52I 122 CGGCCG C^GGCCG
3153 CGGCCG
FspI 1865 TGCGCA TGC^GCA
5057 TGCGCA
KasI 3424 GGCGCC G^GCGCC
3881 GGCGCC
MroI 1997 TCCGGA T^CCGGA
4577 TCCGGA
MseI 3920 TTAA T^TAA
7601 TTAA
NarI 3424 GGCGCC GG^CGCC
3881 GGCGCC
PaeR7I 4525 CTCGAG C^TCGAG
7189 CTCGAG
PshAI 1848 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6984 GACCAGTGTC
SalI 5464 GTCGAC G^TCGAC
7195 GTCGAC
SfoI 3424 GGCGCC GGC^GCC
3881 GGCGCC
Tru9I 3920 TTAA T^TAA
7601 TTAA
XhoI 4525 CTCGAG C^TCGAG
7189 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BclI
BcnI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmnI