Restriction map for pFN21AE3826
 (Circular) (Six-base) MAPSORT of: pFN21AE3826.seq  Check: 1054  from: 1  to: 12718



pFN21AE3826  12718 bp

 With 182 enzymes: SgfI * 

                        July 19, 2019 13:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2779    3269    5760    5877    5978    6045    7290    8220    9284 
   Size:        490    2491     117     101      67    1245     930    1064

Cuts at:   9284    2779 
   Size:       6213

  Fragments arranged by size: 

                6213    2491    1245    1064     930     490     117     101
                  67
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    8088   10528     278 
   Size:         53      83     186     936    6552    2440    2468

  Fragments arranged by size: 

                6552    2468    2440     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    6147    6515    7470    9144    9201   10061    1937 
   Size:       4210     368     955    1674      57     860    4594

  Fragments arranged by size: 

                4594    4210    1674     955     860     368      57
 
Acc65I G'GTAC_C

Cuts at:   4194   10039    4194 
   Size:       5845    6873
 
AclI AA'CG_TT

Cuts at:   1563   10845   11218    1563 
   Size:       9282     373    3063

  Fragments arranged by size: 

                9282    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3590    4223    4621    4795    4913    4931    5233    5615 
   Size:       1958     633     398     174     118      18     302     382

Cuts at:   5615    6427    6842    8237   10781   12165   12433    1632 
   Size:        812     415    1395    2544    1384     268    1917

  Fragments arranged by size: 

                2544    1958    1917    1395    1384     812     633     415
                 398     382     302     268     174     118      18
 
AfeI AGC'GCT

Cuts at:   6914    7355    8777    6914 
   Size:        441    1422   10855

  Fragments arranged by size: 

               10855    1422     441
 
AflII C'TTAA_G

Cuts at:    820    1017    9605     820 
   Size:        197    8588    3933

  Fragments arranged by size: 

                8588    3933     197
 
AflIII A'CryG_T

Cuts at:   1203    2762    3189    3657    4464    7573    8364    9696   11617 
   Size:       1559     427     468     807    3109     791    1332    1921

Cuts at:  11617   12594    1203 
   Size:        977    1327

  Fragments arranged by size: 

                3109    1921    1559    1332    1327     977     807     791
                 468     427
 
AgeI A'CCGG_T

Cuts at:  11549   11549 
   Size:      12718
 
AhdI GACnn_n'nnGTC

Cuts at:   6193    7345   11447    6193 
   Size:       1152    4102    7464

  Fragments arranged by size: 

                7464    4102    1152
 
AleI CACnn'nnGTG

Cuts at:   1166    3209    4784    6227    6278    7601    8699    1166 
   Size:       2043    1575    1443      51    1323    1098    5185

  Fragments arranged by size: 

                5185    2043    1575    1443    1323    1098      51
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3227    3986    4757    5186    5220    6188    6491    7013 
   Size:       2043     759     771     429      34     968     303     522

Cuts at:   7013    7076    7088    7619    1184 
   Size:         63      12     531    6283

  Fragments arranged by size: 

                6283    2043     968     771     759     531     522     429
                 303      63      34      12
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3875    3907    6455    6487    7085    7117    8351    8383    9053 
   Size:         32    2548      32     598      32    1234      32     670

Cuts at:   9053    9085    3875 
   Size:         32    7508

  Fragments arranged by size: 

                7508    2548    1234     670     598      32      32      32
                  32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   2281    2377    2584    3430    3526    4267    4381    5045    5296 
   Size:         96     207     846      96     741     114     664     251

Cuts at:   5296    5698    5737    5902    6508    7021    7642    7735    8077 
   Size:        402      39     165     606     513     621      93     342

Cuts at:   8077    8212    8443   10461   12033    2281 
   Size:        135     231    2018    1572    2966

  Fragments arranged by size: 

                2966    2018    1572     846     741     664     621     606
                 513     402     342     251     231     207     165     135
                 114      96      96      93      39
 
ApaI G_GGCC'C

Cuts at:   4698    7393    7541    7856    8844    9761    4698 
   Size:       2695     148     315     988     917    7655

  Fragments arranged by size: 

                7655    2695     988     917     315     148
 
ApaLI G'TGCA_C

Cuts at:   4355    5864    6163   10775   11931    4355 
   Size:       1509     299    4612    1156    5142

  Fragments arranged by size: 

                5142    4612    1509    1156     299
 
ApoI r'AATT_y

Cuts at:   1493    8960   10027   10253   10405   12514    1493 
   Size:       7467    1067     226     152    2109    1697

  Fragments arranged by size: 

                7467    2109    1697    1067     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032   11272     160 
   Size:        872   10240    1606

  Fragments arranged by size: 

               10240    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      12718
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4470    5218    6351    6530    9194    9480   10043 
   Size:         73    2527     748    1133     179    2664     286     563

Cuts at:  10043    1870 
   Size:       4545

  Fragments arranged by size: 

                4545    2664    2527    1133     748     563     286     179
                  73
 
AvrII C'CTAG_G

Cuts at:   9982    9982 
   Size:      12718
 
BamHI G'GATC_C

Cuts at:   8736   10048   10415    8736 
   Size:       1312     367   11039

  Fragments arranged by size: 

               11039    1312     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3174    3257    4194    5222    5429 
   Size:        325     217     627    1387      83     937    1028     207

Cuts at:   5429    5886    8861    8918    9316   10039   11494     618 
   Size:        457    2975      57     398     723    1455    1842

  Fragments arranged by size: 

                2975    1842    1455    1387    1028     937     723     627
                 457     398     325     217     207      83      57
 
BanII G_rGCy'C

Cuts at:   1391    2066    2124    2607    3888    3957    4698    5778    6566 
   Size:        675      58     483    1281      69     741    1080     788

Cuts at:   6566    7393    7541    7785    7856    8141    8844    9312    9761 
   Size:        827     148     244      71     285     703     468     449

Cuts at:   9761    9942   10037    1391 
   Size:        181      95    4072

  Fragments arranged by size: 

                4072    1281    1080     827     788     741     703     675
                 483     468     449     285     244     181     148      95
                  71      69      58
 
BbeI G_GCGC'C

Cuts at:   5433    5890    5433 
   Size:        457   12261
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3441    4801    4975    5129    7037    8343     928 
   Size:        971    1542    1360     174     154    1908    1306    5303

  Fragments arranged by size: 

                5303    1908    1542    1360    1306     971     174     154
            
 
BbvCI CC'TCA_GC

Cuts at:   2955    8311    2955 
   Size:       5356    7362
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3073    3107    6481    6515   10909   10943    1903 
   Size:         34    1136      34    3374      34    4394      34    3678

  Fragments arranged by size: 

                4394    3678    3374    1136      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:  10610   11820   10610 
   Size:       1210   11508
 
BclI T'GATC_A

Cuts at:   1548    5331    5964    9783    1548 
   Size:       3783     633    3819    4483

  Fragments arranged by size: 

                4483    3819    3783     633
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2828    3027    3244 
   Size:        107     122      71     631     605    1156     199     217

Cuts at:   3244    4145    6599    9128   11329     136 
   Size:        901    2454    2529    2201    1525

  Fragments arranged by size: 

                2529    2454    2201    1525    1156     901     631     605
                 217     199     122     107      71
 
BglII A'GATC_T

Cuts at:   4254    6294    7248   12713    4254 
   Size:       2040     954    5465    4259

  Fragments arranged by size: 

                5465    4259    2040     954
 
BlpI GC'TnA_GC

Cuts at:   2606    4181    4790    4848    6410    6659    6840    8043    8270 
   Size:       1575     609      58    1562     249     181    1203     227

Cuts at:   8270   10135    2606 
   Size:       1865    5189

  Fragments arranged by size: 

                5189    1865    1575    1562    1203     609     249     227
                 181      58
 
Bme1580I G_kGCm'C

Cuts at:   1790    2186    2901    3016    3150    3216    3260    3309    4359 
   Size:        396     715     115     134      66      44      49    1050

Cuts at:   4359    4554    4698    4770    4819    5227    5868    6167    7393 
   Size:        195     144      72      49     408     641     299    1226

Cuts at:   7393    7541    7560    7598    7856    8427    8844    8866    9761 
   Size:        148      19      38     258     571     417      22     895

Cuts at:   9761   10779   11935    1790 
   Size:       1018    1156    2573

  Fragments arranged by size: 

                2573    1226    1156    1050    1018     895     715     641
                 571     417     408     396     299     258     195     148
                 144     134     115      72      66      49      49      44
                  38      22      19
 
BmgBI CAC'GTC

Cuts at:   1318    2722    2763    5314    6190    8367    1318 
   Size:       1404      41    2551     876    2177    5669

  Fragments arranged by size: 

                5669    2551    2177    1404     876      41
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2961    7171    8414 
   Size:        416      53      83     399      24    1544    4210    1243

Cuts at:   8414   10449   11407     442 
   Size:       2035     958    1753

  Fragments arranged by size: 

                4210    2035    1753    1544    1243     958     416     399
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:      12718
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   8275    8275 
   Size:      12718
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2453    2717    3638    5159    5801    6203    6688    6830 
   Size:       1214     264     921    1521     642     402     485     142

Cuts at:   6830    6911    7073    8170    9653    9707    9863   11378    1239 
   Size:         81     162    1097    1483      54     156    1515    2579

  Fragments arranged by size: 

                2579    1521    1515    1483    1214    1097     921     642
                 485     402     264     162     156     142      81      54
            
 
Bpu10I CC'TnA_GC

Cuts at:   2955    4052    6876    8311    2955 
   Size:       1097    2824    1435    7362

  Fragments arranged by size: 

                7362    2824    1435    1097
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   7010    7964    8288    9161    9926   10843   11708   12006    7010 
   Size:        954     324     873     765     917     865     298    7722

  Fragments arranged by size: 

                7722     954     917     873     865     765     324     298
            
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3318    3845    4895    5221    5783    7394    7823 
   Size:        617    1819     527    1050     326     562    1611     429

Cuts at:   7823   11381     882 
   Size:       3558    2219

  Fragments arranged by size: 

                3558    2219    1819    1611    1050     617     562     527
                 429     326
 
BsaAI yAC'GTr

Cuts at:    493    1204    3192    4089    4354    4360    6268    6406    8144 
   Size:        711    1988     897     265       6    1908     138    1738

Cuts at:   8144    8974    9772   10509     493 
   Size:        830     798     737    2702

  Fragments arranged by size: 

                2702    1988    1908    1738     897     830     798     737
                 711     265     138       6
 
BsaBI GATnn'nnATC

Cuts at:   5672    8741    9386   12712    5672 
   Size:       3069     645    3326    5678

  Fragments arranged by size: 

                5678    3326    3069     645
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3345    5430    5887    8085 
   Size:         53      83     186     936    1812    2085     457    2198

Cuts at:   8085   10525   10907   11542     275 
   Size:       2440     382     635    1451

  Fragments arranged by size: 

                2440    2198    2085    1812    1451     936     635     457
                 382     186      83      53
 
BsaWI w'CCGG_w

Cuts at:   4002    6582    7095   11151   11549   11823   11970   12381    4002 
   Size:       2580     513    4056     398     274     147     411    4339

  Fragments arranged by size: 

                4339    4056    2580     513     411     398     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2030    2060    2690    2709    2720 
   Size:         30     165      30     627      30     630      19      11

Cuts at:   2720    2739    3335    3365    3611    3641    3875    3905    4241 
   Size:         19     596      30     246      30     234      30     336

Cuts at:   4241    4271    5093    5123    6548    6578    6971    7001    7391 
   Size:         30     822      30    1425      30     393      30     390

Cuts at:   7391    7421    7976    8006    8513    8543    8579    8609    8675 
   Size:         30     555      30     507      30      36      30      66

Cuts at:   8675    8705    9053    9083    9137    9167    9206    9236    1178 
   Size:         30     348      30      54      30      39      30    4660

  Fragments arranged by size: 

                4660    1425     822     630     627     596     555     507
                 393     390     348     336     246     234     165      66
                  54      39      36      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      19      19      11
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2714    2904    2972    3242    3245    3635    4580    4889 
   Size:       1528     190      68     270       3     390     945     309

Cuts at:   4889    5429    5480    6518    6827    6992    7507    8255    8258 
   Size:        540      51    1038     309     165     515     748       3

Cuts at:   8258    8342    8345    9074    9611    1186 
   Size:         84       3     729     537    4293

  Fragments arranged by size: 

                4293    1528    1038     945     748     729     540     537
                 515     390     309     309     270     190     165      84
                  68      51       3       3       3
 
BseYI C'CCAG_C

Cuts at:   2894    3041    3253    4260    4267    4669    4820    4832    6690 
   Size:        147     212    1007       7     402     151      12    1858

Cuts at:   6690    7392    7534    7541    7777    9475    9927   11921   12417 
   Size:        702     142       7     236    1698     452    1994     496

Cuts at:  12417    2894 
   Size:       3195

  Fragments arranged by size: 

                3195    1994    1858    1698    1007     702     496     452
                 402     236     212     151     147     142      12       7
                   7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2363    3166    3785    4334    4709    5462    5726 
   Size:        573     627     803     619     549     375     753     264

Cuts at:   5726    5733    5738    5897    5915    6521    6699    6851    7355 
   Size:          7       5     159      18     606     178     152     504

Cuts at:   7355    7598    8522    1163 
   Size:        243     924    5359

  Fragments arranged by size: 

                5359     924     803     753     627     619     606     573
                 549     504     375     264     243     178     159     152
                  18       7       5
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2130    5161    7378    9205   10168   10929 
   Size:        275     186     129    3031    2217    1827     963     761

Cuts at:  10929   11957    1540 
   Size:       1028    2301

  Fragments arranged by size: 

                3031    2301    2217    1827    1028     963     761     275
                 186     129
 
BsiHKAI G_wGCw'C

Cuts at:   2607    3206    3723    3957    4182    4359    5181    5868    6167 
   Size:        599     517     234     225     177     822     687     299

Cuts at:   6167    7608    9693    9942   10779   10864   11935    2607 
   Size:       1441    2085     249     837      85    1071    3390

  Fragments arranged by size: 

                3390    2085    1441    1071     837     822     687     599
                 517     299     249     234     225     177      85
 
BsiWI C'GTAC_G

Cuts at:   3159    3159 
   Size:      12718
 
BsmI GAATG_Cn'

Cuts at:   3043    3564    3688    8232   10229   10322    3043 
   Size:        521     124    4544    1997      93    5439

  Fragments arranged by size: 

                5439    4544    1997     521     124      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2050    2712    4412    7715    9056    2050 
   Size:        662    1700    3303    1341    5712

  Fragments arranged by size: 

                5712    3303    1700    1341     662
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2066    2124    2186    2607    2901    3016    3150 
   Size:        399     276      58      62     421     294     115     134

Cuts at:   3150    3206    3216    3260    3309    3723    3888    3957    4182 
   Size:         56      10      44      49     414     165      69     225

Cuts at:   4182    4359    4554    4698    4770    4819    5181    5227    5778 
   Size:        177     195     144      72      49     362      46     551

Cuts at:   5778    5868    6167    6566    7393    7541    7560    7598    7608 
   Size:         90     299     399     827     148      19      38      10

Cuts at:   7608    7785    7856    8141    8427    8844    8866    9312    9693 
   Size:        177      71     285     286     417      22     446     381

Cuts at:   9693    9761    9942   10037   10779   10864   11935    1391 
   Size:         68     181      95     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     827     742     551     446     421     417
                 414     399     399     381     362     299     294     286
                 285     276     225     195     181     177     177     165
                 148     144     134     115      95      90      85      72
                  71      69      68      62      58      56      49      49
                  46      44      38      22      19      10      10
 
BspEI T'CCGG_A

Cuts at:   4002    6582   12381    4002 
   Size:       2580    5799    4339

  Fragments arranged by size: 

                5799    4339    2580
 
BspHI T'CATG_A

Cuts at:   4114    5967    9013    9399   10607   12337    4114 
   Size:       1853    3046     386    1208    1730    4495

  Fragments arranged by size: 

                4495    3046    1853    1730    1208     386
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2779    3269    3402    4329    4980    5275    5637    5760 
   Size:       1935     490     133     927     651     295     362     123

Cuts at:   5760    5877    5978    6045    6123    7290    7674    8112    8220 
   Size:        117     101      67      78    1167     384     438     108

Cuts at:   8220    9284    9579   10073     844 
   Size:       1064     295     494    3489

  Fragments arranged by size: 

                3489    1935    1167    1064     927     651     494     490
                 438     384     362     295     295     133     123     117
                 108     101      78      67
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3235    6817    9105    9361   10605    1394 
   Size:        429    1412    3582    2288     256    1244    3507

  Fragments arranged by size: 

                3582    3507    2288    1412    1244     429     256
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3091    3723    8658    8723    9103    9847   11213 
   Size:       1183    1843     632    4935      65     380     744    1366

Cuts at:  11213   11387      65 
   Size:        174    1396

  Fragments arranged by size: 

                4935    1843    1396    1366    1183     744     632     380
                 174      65
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2618    4154    4390    5349    7182    8022    8221 
   Size:        110     808    1536     236     959    1833     840     199

Cuts at:   8221    8463    9204   11362   11549    1700 
   Size:        242     741    2158     187    2869

  Fragments arranged by size: 

                2869    2158    1833    1536     959     840     808     741
                 242     236     199     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:      12718
 
BssHII G'CGCG_C

Cuts at:   1925    4324    1925 
   Size:       2399   10319
 
BssSI C'ACGA_G

Cuts at:   4043    7559    7889   10778   11790    4043 
   Size:       3516     330    2889    1012    4971

  Fragments arranged by size: 

                4971    3516    2889    1012     330
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2281    2584    5590    5863    6508    7018    7021    8212 
   Size:       1487     303    3006     273     645     510       3    1191

Cuts at:   8212     794 
   Size:       5300

  Fragments arranged by size: 

                5300    3006    1487    1191     645     510     303     273
                   3
 
BstBI TT'CG_AA

Cuts at:  11522   11522 
   Size:      12718
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:      12718
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3370    3895    4504    5668    6634    7210    8950    8998    9238 
   Size:        525     609    1164     966     576    1740      48     240

Cuts at:   9238    9907    3370 
   Size:        669    6181

  Fragments arranged by size: 

                6181    1740    1164     966     669     609     576     525
                 240      48
 
BstYI r'GATC_y

Cuts at:   1972    2989    3318    4254    4419    4914    5637    5673    6294 
   Size:       1017     329     936     165     495     723      36     621

Cuts at:   6294    7248    7305    7963    8736   10048   10415   10800   10817 
   Size:        954      57     658     773    1312     367     385      17

Cuts at:  10817   12269   12355   12367   12713    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1312    1017     954     936     773     723
                 658     621     495     385     367     346     329     165
                  86      57      36      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   9321    9321 
   Size:      12718
 
BtgI C'CryG_G

Cuts at:    513    1065    2124    2884    3369    4503    4782    5361    5457 
   Size:        552    1059     760     485    1134     279     579      96

Cuts at:   5457    6539    6567    6594    6627    7410    7758    8914    8943 
   Size:       1082      28      27      33     783     348    1156      29

Cuts at:   8943    9406     513 
   Size:        463    3825

  Fragments arranged by size: 

                3825    1156    1134    1082    1059     783     760     579
                 552     485     463     348     279      96      33      29
                  28      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3076    3471    3916    6009    8740     487 
   Size:        683      56    1850     395     445    2093    2731    4465

  Fragments arranged by size: 

                4465    2731    2093    1850     683     445     395      56
            
 
BtsI GCAGTG_nn'

Cuts at:   2368    2807    3789    4664    5061    5407    5506    6259    6670 
   Size:        439     982     875     397     346      99     753     411

Cuts at:   6670    6904    7591    9286    9921   10234   11028   11048    2368 
   Size:        234     687    1695     635     313     794      20    4038

  Fragments arranged by size: 

                4038    1695     982     875     794     753     687     635
                 439     411     397     346     313     234      99      20
            
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:      12718
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    9406    9441     529 
   Size:         35    8842      35    3806

  Fragments arranged by size: 

                8842    3806      35      35
 
DraI TTT'AAA

Cuts at:  10022   10022 
   Size:      12718
 
DraIII CAC_nnn'GTG

Cuts at:   5863    7179    7564    5863 
   Size:       1316     385   11017

  Fragments arranged by size: 

               11017    1316     385
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2534    3494    5999    7891   11725     809 
   Size:        507    1218     960    2505    1892    3834    1802

  Fragments arranged by size: 

                3834    2505    1892    1802    1218     960     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2127    2829    3360 
   Size:         54    1425      50     275      18     297     702     531

Cuts at:   3360    3774    4242    4701    5158    5874    6120    6600    7272 
   Size:        414     468     459     457     716     246     480     672

Cuts at:   7272    8637    9100   10165   11054   12683       8 
   Size:       1365     463    1065     889    1629      43

  Fragments arranged by size: 

                1629    1425    1365    1065     889     716     702     672
                 531     480     468     463     459     457     414     297
                 275     246      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2127    5158   10165    1537 
   Size:        275     315    3031    5007    4090

  Fragments arranged by size: 

                5007    4090    3031     315     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2177    2381    2495    4065    4115    5243    5765    6890 
   Size:        824     204     114    1570      50    1128     522    1125

Cuts at:   6890    8109    8266    8474    8735    9155    9176    9327    9476 
   Size:       1219     157     208     261     420      21     151     149

Cuts at:   9476   10648    1353 
   Size:       1172    3423

  Fragments arranged by size: 

                3423    1570    1219    1172    1128    1125     824     522
                 420     261     208     204     157     151     149     114
                  50      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3653    4937    5114    8777   11305   11679 
   Size:       1064     138    2064    1284     177    3663    2528     374

Cuts at:  11679   11825     387 
   Size:        146    1280

  Fragments arranged by size: 

                3663    2528    2064    1284    1280    1064     374     177
                 146     138
 
EcoICRI GAG'CTC

Cuts at:   2605    3955    9940    2605 
   Size:       1350    5985    5383

  Fragments arranged by size: 

                5985    5383    1350
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2453    2717    3590    3638    4223    4621    4795 
   Size:        393     821     264     873      48     585     398     174

Cuts at:   4795    4913    4931    5159    5233    5615    5801    6203    6427 
   Size:        118      18     228      74     382     186     402     224

Cuts at:   6427    6688    6830    6842    6911    7073    8170    8237    9653 
   Size:        261     142      12      69     162    1097      67    1416

Cuts at:   9653    9707    9863   10781   11378   12165   12433    1239 
   Size:         54     156     918     597     787     268    1524

  Fragments arranged by size: 

                1524    1416    1097     918     873     821     787     597
                 585     402     398     393     382     268     264     261
                 228     224     186     174     162     156     142     118
                  74      69      67      54      48      18      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2174    2699    3551    3776    6833    8108    8447    9332    2174 
   Size:        525     852     225    3057    1275     339     885    5560

  Fragments arranged by size: 

                5560    3057    1275     885     852     525     339     225
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2134    3574    3602    4694    4695    4723    5734    6961    7117 
   Size:       1440      28    1092       1      28    1011    1227     156

Cuts at:   7117    7538    7663    7852    7853    9325    9758    2134 
   Size:        421     125     189       1    1472     433    5094

  Fragments arranged by size: 

                5094    1472    1440    1227    1092    1011     433     421
                 189     156     125      28      28       1       1
 
EcoRI G'AATT_C

Cuts at:   8960   10027   10405    8960 
   Size:       1067     378   11273

  Fragments arranged by size: 

               11273    1067     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3304    9202    3304 
   Size:       5898    6820
 
FspI TGC'GCA

Cuts at:   3872    7064   11224    3872 
   Size:       3192    4160    5366

  Fragments arranged by size: 

                5366    4160    3192
 
HaeII r_GCGC'y

Cuts at:   1678    5260    5433    5890    6916    7357    8177    8318    8779 
   Size:       3582     173     457    1026     441     820     141     461

Cuts at:   8779    8807   11865    1678 
   Size:         28    3058    2531

  Fragments arranged by size: 

                3582    3058    2531    1026     820     461     457     441
                 173     141      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2030    2062    2545    2577    3080 
   Size:         32     135      32     458      32     483      32     503

Cuts at:   3080    3112    3335    3367    3611    3643    3890    3922    4241 
   Size:         32     223      32     244      32     247      32     319

Cuts at:   4241    4273    6008    6040    6179    6211    7331    7363    7391 
   Size:         32    1735      32     139      32    1120      32      28

Cuts at:   7391    7423    7976    8008    8513    8545    8675    8707    9287 
   Size:         32     553      32     505      32     130      32     580

Cuts at:   9287    9319   11359   11391   11433   11465    1373 
   Size:         32    2040      32      42      32    2626

  Fragments arranged by size: 

                2626    2040    1735    1120     580     553     505     503
                 483     458     319     247     244     223     139     135
                 130      42      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      28
 
HincII GTy'rAC

Cuts at:    669    1657    1938    7423    7471    9202   10062   10308     669 
   Size:        988     281    5485      48    1731     860     246    3079

  Fragments arranged by size: 

                5485    3079    1731     988     860     281     246      48
            
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:      12718
 
HpaI GTT'AAC

Cuts at:  10308   10308 
   Size:      12718
 
KasI G'GCGC_C

Cuts at:   5429    5886    5429 
   Size:        457   12261
 
KpnI G_GTAC'C

Cuts at:   4198   10043    4198 
   Size:       5845    6873
 
MfeI C'AATT_G

Cuts at:  10317   10317 
   Size:      12718
 
MluI A'CGCG_T

Cuts at:   4464   12594    4464 
   Size:       8130    4588
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3948    4479    5319    6954   11832   12016    1305 
   Size:       2643     531     840    1635    4878     184    2007

  Fragments arranged by size: 

                4878    2643    2007    1635     840     531     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3362    4244    5876    6602    7274 
   Size:         54    1425     343    1530     882    1632     726     672

Cuts at:   7274      10 
   Size:       5454

  Fragments arranged by size: 

                5454    1632    1530    1425     882     726     672     343
                  54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2343    2450    2904    3084    3209    4784    4892 
   Size:        648    1177     107     454     180     125    1575     108

Cuts at:   4892    5202    5966    6227    6278    6995    7601    8031    8699 
   Size:        310     764     261      51     717     606     430     668

Cuts at:   8699    8948    8954    9398    9524   10676   11035   11194     518 
   Size:        249       6     444     126    1152     359     159    2042

  Fragments arranged by size: 

                2042    1575    1177    1152     764     717     668     648
                 606     454     444     430     359     310     261     249
                 180     159     126     125     108     107      51       6
            
 
MspA1I CmG'CkG

Cuts at:   1606    2109    2126    2658    3427    4135    4201    4267    4855 
   Size:        503      17     532     769     708      66      66     588

Cuts at:   4855    5212    5233    5293    6181    6505    6675    6683    6694 
   Size:        357      21      60     888     324     170       8      11

Cuts at:   6694    7060    7639    8203    8290    8581    9964   10134   10811 
   Size:        366     579     564      87     291    1383     170     677

Cuts at:  10811   11959   12204    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    1383    1148     888     769     708     677     588
                 579     564     532     503     366     357     324     291
                 245     170     170      87      66      66      60      21
                  17      11       8
 
NaeI GCC'GGC

Cuts at:   2620    4156    7184    8024    2620 
   Size:       1536    3028     840    7314

  Fragments arranged by size: 

                7314    3028    1536     840
 
NarI GG'CG_CC

Cuts at:   5430    5887    5430 
   Size:        457   12261
 
NcoI C'CATG_G

Cuts at:    513    1065    4503    4782    5361    6594    6627    8943    9406 
   Size:        552    3438     279     579    1233      33    2316     463

Cuts at:   9406     513 
   Size:       3825

  Fragments arranged by size: 

                3825    3438    2316    1233     579     552     463     279
                  33
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:      12718
 
NgoMIV G'CCGG_C

Cuts at:   2618    4154    7182    8022    2618 
   Size:       1536    3028     840    7314

  Fragments arranged by size: 

                7314    3028    1536     840
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:      12718
 
NotI GC'GGCC_GC

Cuts at:   5158   10165    5158 
   Size:       5007    7711
 
NspI r_CATG'y

Cuts at:   1132    1229    2291    2591    3661    4222    5137    5167    8578 
   Size:         97    1062     300    1070     561     915      30    3411

Cuts at:   8578    9618    9700   10076   11621    1132 
   Size:       1040      82     376    1545    2229

  Fragments arranged by size: 

                3411    2229    1545    1070    1062    1040     915     561
                 376     300      97      82      30
 
PasI CC'CwG_GG

Cuts at:   1348    9238    1348 
   Size:       7890    4828
 
PciI A'CATG_T

Cuts at:   3657    9696   11617    3657 
   Size:       6039    1921    4758

  Fragments arranged by size: 

                6039    4758    1921
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3192    3430    4240    4267    4381    4762    6040    6759 
   Size:       2084     238     810      27     114     381    1278     719

Cuts at:   6759    7285    7825    8077    1108 
   Size:        526     540     252    5749

  Fragments arranged by size: 

                5749    2084    1278     810     719     540     526     381
                 252     238     114      27
 
PfoI T'CCnGG_A

Cuts at:   3483    3819    3894    4917    5529    5778    6888    7254    7734 
   Size:        336      75    1023     612     249    1110     366     480

Cuts at:   7734    9327    9879    3483 
   Size:       1593     552    6322

  Fragments arranged by size: 

                6322    1593    1110    1023     612     552     480     366
                 336     249      75
 
PmeI GTTT'AAAC

Cuts at:  10022   10022 
   Size:      12718
 
PmlI CAC'GTG

Cuts at:   3192    4089    4354    4360    8144    8974    3192 
   Size:        897     265       6    3784     830    6936

  Fragments arranged by size: 

                6936    3784     897     830     265       6
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2183    2215    3875    3907    9053    9085   10787   10819   12300 
   Size:         32    1660      32    5146      32    1702      32    1481

Cuts at:  12300   12332    2183 
   Size:         32    2569

  Fragments arranged by size: 

                5146    2569    1702    1660    1481      32      32      32
                  32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2134    3602    4723    6961    7117    7663    9325    2134 
   Size:       1468    1121    2238     156     546    1662    5527

  Fragments arranged by size: 

                5527    2238    1662    1468    1121     546     156
 
PshAI GACnn'nnGTC

Cuts at:   1613    3857    8993    1613 
   Size:       2244    5136    5338

  Fragments arranged by size: 

                5338    5136    2244
 
PsiI TTA'TAA

Cuts at:  10288   10288 
   Size:      12718
 
PspOMI G'GGCC_C

Cuts at:   4694    7389    7537    7852    8840    9757    4694 
   Size:       2695     148     315     988     917    7655

  Fragments arranged by size: 

                7655    2695     988     917     315     148
 
PspXI vC'TCGA_Gb

Cuts at:   6530    6530 
   Size:      12718
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2954    2986    3632    3664    6248    6280    9695    9727    2954 
   Size:         32     646      32    2584      32    3415      32    5945

  Fragments arranged by size: 

                5945    3415    2584     646      32      32      32      32
            
 
PstI C_TGCA'G

Cuts at:    830    2277    2286    2790    4713    5232    5637    5703    6362 
   Size:       1447       9     504    1923     519     405      66     659

Cuts at:   6362    6480    6513    6792    7014    7638    7671    7731    7977 
   Size:        118      33     279     222     624      33      60     246

Cuts at:   7977    8109    8181    8208    9281   10070     830 
   Size:        132      72      27    1073     789    3478

  Fragments arranged by size: 

                3478    1923    1447    1073     789     659     624     519
                 504     405     279     246     222     132     118      72
                  66      60      33      33      27       9
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:      12718
 
PvuII CAG'CTG

Cuts at:   2658    3427    4135    4201    4267    5233    5293    6505    6675 
   Size:        769     708      66      66     966      60    1212     170

Cuts at:   6675    6683    7060    7639    8203    8290    2658 
   Size:          8     377     579     564      87    7086

  Fragments arranged by size: 

                7086    1212     966     769     708     579     564     377
                 170      87      66      66      60       8
 
RsrII CG'GwC_CG

Cuts at:   4005    4005 
   Size:      12718
 
SacI G_AGCT'C

Cuts at:   2607    3957    9942    2607 
   Size:       1350    5985    5383

  Fragments arranged by size: 

                5985    5383    1350
 
SacII CC_GC'GG

Cuts at:   2127    2127 
   Size:      12718
 
SalI G'TCGA_C

Cuts at:   1936    7469    9200   10060    1936 
   Size:       5533    1731     860    4594

  Fragments arranged by size: 

                5533    4594    1731     860
 
SapI GCTCTTCn'nnn_

Cuts at:   2381    2495    5243    6890    8109    8474    8735    2381 
   Size:        114    2748    1647    1219     365     261    6364

  Fragments arranged by size: 

                6364    2748    1647    1219     365     261     114
 
SbfI CC_TGCA'GG

Cuts at:   8109   10070    8109 
   Size:       1961   10757
 
ScaI AGT'ACT

Cuts at:   2216    3059    4064    4238    7844   10966   12678    2216 
   Size:        843    1005     174    3606    3122    1712    2256

  Fragments arranged by size: 

                3606    3122    2256    1712    1005     843     174
 
SexAI A'CCwGG_T

Cuts at:   3438    3438 
   Size:      12718
 
SfcI C'TryA_G

Cuts at:    826    1045    2273    2282    2786    2934    4189    4630    4709 
   Size:        219    1228       9     504     148    1255     441      79

Cuts at:   4709    5228    5633    5699    6358    6476    6509    6545    6788 
   Size:        519     405      66     659     118      33      36     243

Cuts at:   6788    7010    7634    7667    7727    7973    8105    8177    8204 
   Size:        222     624      33      60     246     132      72      27

Cuts at:   8204    8779    9025    9277   10066   11201   11882   12073     826 
   Size:        575     246     252     789    1135     681     191    1471

  Fragments arranged by size: 

                1471    1255    1228    1135     789     681     659     624
                 575     519     504     441     405     252     246     246
                 243     222     219     191     148     132     118      79
                  72      66      60      36      33      33      27       9
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   3027    3027 
   Size:      12718
 
SfoI GGC'GCC

Cuts at:   5431    5888    5431 
   Size:        457   12261
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      12718
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:      12718
 
SmaI CCC'GGG

Cuts at:   1872   10045    1872 
   Size:       8173    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    6530    7025    7979    8303    9176    9194 
   Size:        197     926    4587     495     954     324     873      18

Cuts at:   9194    9605    9941   10822   11723   11985     820 
   Size:        411     336     881     901     262    1553

  Fragments arranged by size: 

                4587    1553     954     926     901     881     873     495
                 411     336     324     262     197      18
 
SnaBI TAC'GTA

Cuts at:    493   10509     493 
   Size:      10016    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:      12718
 
SphI G_CATG'C

Cuts at:   1132    2291    2591    5137    8578   10076    1132 
   Size:       1159     300    2546    3441    1498    3774

  Fragments arranged by size: 

                3774    3441    2546    1498    1159     300
 
SspI AAT'ATT

Cuts at:      5      52   10642       5 
   Size:         47   10590    2081

  Fragments arranged by size: 

               10590    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3287    4715    6575    6905    8099    1517 
   Size:       1770    1428    1860     330    1194    6136

  Fragments arranged by size: 

                6136    1860    1770    1428    1194     330
 
StyI C'CwwG_G

Cuts at:    513    1065    2994    3270    4503    4782    5361    5436    6393 
   Size:        552    1929     276    1233     279     579      75     957

Cuts at:   6393    6594    6603    6627    6957    7824    8943    9406    9982 
   Size:        201       9      24     330     867    1119     463     576

Cuts at:   9982   10157     513 
   Size:        175    3074

  Fragments arranged by size: 

                3074    1929    1233    1119     957     867     579     576
                 552     463     330     279     276     201     175      75
                  24       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3857    3962    4022    5374    5496    7190    8849 
   Size:       1255    2277     105      60    1352     122    1694    1659

Cuts at:   8849    9761   10745   10762   10915   11100   11545     325 
   Size:        912     984      17     153     185     445    1498

  Fragments arranged by size: 

                2277    1694    1659    1498    1352    1255     984     912
                 445     185     153     122     105      60      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2214    2772    3057 
   Size:        274      80      33      51    1443     237     558     285

Cuts at:   3057    4062    4236    7842    9711   10964   12676      96 
   Size:       1005     174    3606    1869    1253    1712     138

  Fragments arranged by size: 

                3606    1869    1712    1443    1253    1005     558     285
                 274     237     174     138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2709    2741    4760    4792    6548    6580    6971    7003    7730 
   Size:         32    2019      32    1756      32     391      32     727

Cuts at:   7730    7762    8838    8870   12678   12710    2709 
   Size:         32    1076      32    3808      32    2717

  Fragments arranged by size: 

                3808    2717    2019    1756    1076     727     391      32
                  32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2042    7405    9299    9661    1316 
   Size:        726    5363    1894     362    4373

  Fragments arranged by size: 

                5363    4373    1894     726     362
 
XbaI T'CTAG_A

Cuts at:  10054   10054 
   Size:      12718
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3355    3844    4510    4759    5074    5113    6964    7282    7286 
   Size:        489     666     249     315      39    1851     318       4

Cuts at:   7286    7411    7489    7708    7765    8002    8701    8827    9904 
   Size:        125      78     219      57     237     699     126    1077

Cuts at:   9904    3355 
   Size:       6169

  Fragments arranged by size: 

                6169    1851    1077     699     666     489     318     315
                 249     237     219     126     125      78      57      39
                   4
 
XhoI C'TCGA_G

Cuts at:   1943    6530    9194    1943 
   Size:       4587    2664    5467

  Fragments arranged by size: 

                5467    4587    2664
 
XmaI C'CCGG_G

Cuts at:   1870   10043    1870 
   Size:       8173    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4926    7250    7316   10847    4926 
   Size:       2324      66    3531    6797

  Fragments arranged by size: 

                6797    3531    2324      66
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    8086   10526     276 
   Size:         53      83     186     936    6552    2440    2468

  Fragments arranged by size: 

                6552    2468    2440     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI     BaeI    BfrBI  BstZ17I    EcoRV     FseI    FspAI     NruI
     NsiI     PacI    SanDI     SrfI     SwaI
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