(Circular) (Six-base) MAPSORT of: pFN21AE3826.seq Check: 1054 from: 1 to: 12718 pFN21AE3826 12718 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2779 3269 5760 5877 5978 6045 7290 8220 9284 Size: 490 2491 117 101 67 1245 930 1064 Cuts at: 9284 2779 Size: 6213 Fragments arranged by size: 6213 2491 1245 1064 930 490 117 101 67 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8088 10528 278 Size: 53 83 186 936 6552 2440 2468 Fragments arranged by size: 6552 2468 2440 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6147 6515 7470 9144 9201 10061 1937 Size: 4210 368 955 1674 57 860 4594 Fragments arranged by size: 4594 4210 1674 955 860 368 57 Acc65I G'GTAC_C Cuts at: 4194 10039 4194 Size: 5845 6873 AclI AA'CG_TT Cuts at: 1563 10845 11218 1563 Size: 9282 373 3063 Fragments arranged by size: 9282 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3590 4223 4621 4795 4913 4931 5233 5615 Size: 1958 633 398 174 118 18 302 382 Cuts at: 5615 6427 6842 8237 10781 12165 12433 1632 Size: 812 415 1395 2544 1384 268 1917 Fragments arranged by size: 2544 1958 1917 1395 1384 812 633 415 398 382 302 268 174 118 18 AfeI AGC'GCT Cuts at: 6914 7355 8777 6914 Size: 441 1422 10855 Fragments arranged by size: 10855 1422 441 AflII C'TTAA_G Cuts at: 820 1017 9605 820 Size: 197 8588 3933 Fragments arranged by size: 8588 3933 197 AflIII A'CryG_T Cuts at: 1203 2762 3189 3657 4464 7573 8364 9696 11617 Size: 1559 427 468 807 3109 791 1332 1921 Cuts at: 11617 12594 1203 Size: 977 1327 Fragments arranged by size: 3109 1921 1559 1332 1327 977 807 791 468 427 AgeI A'CCGG_T Cuts at: 11549 11549 Size: 12718 AhdI GACnn_n'nnGTC Cuts at: 6193 7345 11447 6193 Size: 1152 4102 7464 Fragments arranged by size: 7464 4102 1152 AleI CACnn'nnGTG Cuts at: 1166 3209 4784 6227 6278 7601 8699 1166 Size: 2043 1575 1443 51 1323 1098 5185 Fragments arranged by size: 5185 2043 1575 1443 1323 1098 51 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3227 3986 4757 5186 5220 6188 6491 7013 Size: 2043 759 771 429 34 968 303 522 Cuts at: 7013 7076 7088 7619 1184 Size: 63 12 531 6283 Fragments arranged by size: 6283 2043 968 771 759 531 522 429 303 63 34 12 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3875 3907 6455 6487 7085 7117 8351 8383 9053 Size: 32 2548 32 598 32 1234 32 670 Cuts at: 9053 9085 3875 Size: 32 7508 Fragments arranged by size: 7508 2548 1234 670 598 32 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2281 2377 2584 3430 3526 4267 4381 5045 5296 Size: 96 207 846 96 741 114 664 251 Cuts at: 5296 5698 5737 5902 6508 7021 7642 7735 8077 Size: 402 39 165 606 513 621 93 342 Cuts at: 8077 8212 8443 10461 12033 2281 Size: 135 231 2018 1572 2966 Fragments arranged by size: 2966 2018 1572 846 741 664 621 606 513 402 342 251 231 207 165 135 114 96 96 93 39 ApaI G_GGCC'C Cuts at: 4698 7393 7541 7856 8844 9761 4698 Size: 2695 148 315 988 917 7655 Fragments arranged by size: 7655 2695 988 917 315 148 ApaLI G'TGCA_C Cuts at: 4355 5864 6163 10775 11931 4355 Size: 1509 299 4612 1156 5142 Fragments arranged by size: 5142 4612 1509 1156 299 ApoI r'AATT_y Cuts at: 1493 8960 10027 10253 10405 12514 1493 Size: 7467 1067 226 152 2109 1697 Fragments arranged by size: 7467 2109 1697 1067 226 152 AseI AT'TA_AT Cuts at: 160 1032 11272 160 Size: 872 10240 1606 Fragments arranged by size: 10240 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12718 AvaI C'yCGr_G Cuts at: 1870 1943 4470 5218 6351 6530 9194 9480 10043 Size: 73 2527 748 1133 179 2664 286 563 Cuts at: 10043 1870 Size: 4545 Fragments arranged by size: 4545 2664 2527 1133 748 563 286 179 73 AvrII C'CTAG_G Cuts at: 9982 9982 Size: 12718 BamHI G'GATC_C Cuts at: 8736 10048 10415 8736 Size: 1312 367 11039 Fragments arranged by size: 11039 1312 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3174 3257 4194 5222 5429 Size: 325 217 627 1387 83 937 1028 207 Cuts at: 5429 5886 8861 8918 9316 10039 11494 618 Size: 457 2975 57 398 723 1455 1842 Fragments arranged by size: 2975 1842 1455 1387 1028 937 723 627 457 398 325 217 207 83 57 BanII G_rGCy'C Cuts at: 1391 2066 2124 2607 3888 3957 4698 5778 6566 Size: 675 58 483 1281 69 741 1080 788 Cuts at: 6566 7393 7541 7785 7856 8141 8844 9312 9761 Size: 827 148 244 71 285 703 468 449 Cuts at: 9761 9942 10037 1391 Size: 181 95 4072 Fragments arranged by size: 4072 1281 1080 827 788 741 703 675 483 468 449 285 244 181 148 95 71 69 58 BbeI G_GCGC'C Cuts at: 5433 5890 5433 Size: 457 12261 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3441 4801 4975 5129 7037 8343 928 Size: 971 1542 1360 174 154 1908 1306 5303 Fragments arranged by size: 5303 1908 1542 1360 1306 971 174 154 BbvCI CC'TCA_GC Cuts at: 2955 8311 2955 Size: 5356 7362 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3073 3107 6481 6515 10909 10943 1903 Size: 34 1136 34 3374 34 4394 34 3678 Fragments arranged by size: 4394 3678 3374 1136 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 10610 11820 10610 Size: 1210 11508 BclI T'GATC_A Cuts at: 1548 5331 5964 9783 1548 Size: 3783 633 3819 4483 Fragments arranged by size: 4483 3819 3783 633 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2828 3027 3244 Size: 107 122 71 631 605 1156 199 217 Cuts at: 3244 4145 6599 9128 11329 136 Size: 901 2454 2529 2201 1525 Fragments arranged by size: 2529 2454 2201 1525 1156 901 631 605 217 199 122 107 71 BglII A'GATC_T Cuts at: 4254 6294 7248 12713 4254 Size: 2040 954 5465 4259 Fragments arranged by size: 5465 4259 2040 954 BlpI GC'TnA_GC Cuts at: 2606 4181 4790 4848 6410 6659 6840 8043 8270 Size: 1575 609 58 1562 249 181 1203 227 Cuts at: 8270 10135 2606 Size: 1865 5189 Fragments arranged by size: 5189 1865 1575 1562 1203 609 249 227 181 58 Bme1580I G_kGCm'C Cuts at: 1790 2186 2901 3016 3150 3216 3260 3309 4359 Size: 396 715 115 134 66 44 49 1050 Cuts at: 4359 4554 4698 4770 4819 5227 5868 6167 7393 Size: 195 144 72 49 408 641 299 1226 Cuts at: 7393 7541 7560 7598 7856 8427 8844 8866 9761 Size: 148 19 38 258 571 417 22 895 Cuts at: 9761 10779 11935 1790 Size: 1018 1156 2573 Fragments arranged by size: 2573 1226 1156 1050 1018 895 715 641 571 417 408 396 299 258 195 148 144 134 115 72 66 49 49 44 38 22 19 BmgBI CAC'GTC Cuts at: 1318 2722 2763 5314 6190 8367 1318 Size: 1404 41 2551 876 2177 5669 Fragments arranged by size: 5669 2551 2177 1404 876 41 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2961 7171 8414 Size: 416 53 83 399 24 1544 4210 1243 Cuts at: 8414 10449 11407 442 Size: 2035 958 1753 Fragments arranged by size: 4210 2035 1753 1544 1243 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12718 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8275 8275 Size: 12718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2717 3638 5159 5801 6203 6688 6830 Size: 1214 264 921 1521 642 402 485 142 Cuts at: 6830 6911 7073 8170 9653 9707 9863 11378 1239 Size: 81 162 1097 1483 54 156 1515 2579 Fragments arranged by size: 2579 1521 1515 1483 1214 1097 921 642 485 402 264 162 156 142 81 54 Bpu10I CC'TnA_GC Cuts at: 2955 4052 6876 8311 2955 Size: 1097 2824 1435 7362 Fragments arranged by size: 7362 2824 1435 1097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7010 7964 8288 9161 9926 10843 11708 12006 7010 Size: 954 324 873 765 917 865 298 7722 Fragments arranged by size: 7722 954 917 873 865 765 324 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3318 3845 4895 5221 5783 7394 7823 Size: 617 1819 527 1050 326 562 1611 429 Cuts at: 7823 11381 882 Size: 3558 2219 Fragments arranged by size: 3558 2219 1819 1611 1050 617 562 527 429 326 BsaAI yAC'GTr Cuts at: 493 1204 3192 4089 4354 4360 6268 6406 8144 Size: 711 1988 897 265 6 1908 138 1738 Cuts at: 8144 8974 9772 10509 493 Size: 830 798 737 2702 Fragments arranged by size: 2702 1988 1908 1738 897 830 798 737 711 265 138 6 BsaBI GATnn'nnATC Cuts at: 5672 8741 9386 12712 5672 Size: 3069 645 3326 5678 Fragments arranged by size: 5678 3326 3069 645 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3345 5430 5887 8085 Size: 53 83 186 936 1812 2085 457 2198 Cuts at: 8085 10525 10907 11542 275 Size: 2440 382 635 1451 Fragments arranged by size: 2440 2198 2085 1812 1451 936 635 457 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4002 6582 7095 11151 11549 11823 11970 12381 4002 Size: 2580 513 4056 398 274 147 411 4339 Fragments arranged by size: 4339 4056 2580 513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2030 2060 2690 2709 2720 Size: 30 165 30 627 30 630 19 11 Cuts at: 2720 2739 3335 3365 3611 3641 3875 3905 4241 Size: 19 596 30 246 30 234 30 336 Cuts at: 4241 4271 5093 5123 6548 6578 6971 7001 7391 Size: 30 822 30 1425 30 393 30 390 Cuts at: 7391 7421 7976 8006 8513 8543 8579 8609 8675 Size: 30 555 30 507 30 36 30 66 Cuts at: 8675 8705 9053 9083 9137 9167 9206 9236 1178 Size: 30 348 30 54 30 39 30 4660 Fragments arranged by size: 4660 1425 822 630 627 596 555 507 393 390 348 336 246 234 165 66 54 39 36 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2714 2904 2972 3242 3245 3635 4580 4889 Size: 1528 190 68 270 3 390 945 309 Cuts at: 4889 5429 5480 6518 6827 6992 7507 8255 8258 Size: 540 51 1038 309 165 515 748 3 Cuts at: 8258 8342 8345 9074 9611 1186 Size: 84 3 729 537 4293 Fragments arranged by size: 4293 1528 1038 945 748 729 540 537 515 390 309 309 270 190 165 84 68 51 3 3 3 BseYI C'CCAG_C Cuts at: 2894 3041 3253 4260 4267 4669 4820 4832 6690 Size: 147 212 1007 7 402 151 12 1858 Cuts at: 6690 7392 7534 7541 7777 9475 9927 11921 12417 Size: 702 142 7 236 1698 452 1994 496 Cuts at: 12417 2894 Size: 3195 Fragments arranged by size: 3195 1994 1858 1698 1007 702 496 452 402 236 212 151 147 142 12 7 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2363 3166 3785 4334 4709 5462 5726 Size: 573 627 803 619 549 375 753 264 Cuts at: 5726 5733 5738 5897 5915 6521 6699 6851 7355 Size: 7 5 159 18 606 178 152 504 Cuts at: 7355 7598 8522 1163 Size: 243 924 5359 Fragments arranged by size: 5359 924 803 753 627 619 606 573 549 504 375 264 243 178 159 152 18 7 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2130 5161 7378 9205 10168 10929 Size: 275 186 129 3031 2217 1827 963 761 Cuts at: 10929 11957 1540 Size: 1028 2301 Fragments arranged by size: 3031 2301 2217 1827 1028 963 761 275 186 129 BsiHKAI G_wGCw'C Cuts at: 2607 3206 3723 3957 4182 4359 5181 5868 6167 Size: 599 517 234 225 177 822 687 299 Cuts at: 6167 7608 9693 9942 10779 10864 11935 2607 Size: 1441 2085 249 837 85 1071 3390 Fragments arranged by size: 3390 2085 1441 1071 837 822 687 599 517 299 249 234 225 177 85 BsiWI C'GTAC_G Cuts at: 3159 3159 Size: 12718 BsmI GAATG_Cn' Cuts at: 3043 3564 3688 8232 10229 10322 3043 Size: 521 124 4544 1997 93 5439 Fragments arranged by size: 5439 4544 1997 521 124 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2050 2712 4412 7715 9056 2050 Size: 662 1700 3303 1341 5712 Fragments arranged by size: 5712 3303 1700 1341 662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2124 2186 2607 2901 3016 3150 Size: 399 276 58 62 421 294 115 134 Cuts at: 3150 3206 3216 3260 3309 3723 3888 3957 4182 Size: 56 10 44 49 414 165 69 225 Cuts at: 4182 4359 4554 4698 4770 4819 5181 5227 5778 Size: 177 195 144 72 49 362 46 551 Cuts at: 5778 5868 6167 6566 7393 7541 7560 7598 7608 Size: 90 299 399 827 148 19 38 10 Cuts at: 7608 7785 7856 8141 8427 8844 8866 9312 9693 Size: 177 71 285 286 417 22 446 381 Cuts at: 9693 9761 9942 10037 10779 10864 11935 1391 Size: 68 181 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 827 742 551 446 421 417 414 399 399 381 362 299 294 286 285 276 225 195 181 177 177 165 148 144 134 115 95 90 85 72 71 69 68 62 58 56 49 49 46 44 38 22 19 10 10 BspEI T'CCGG_A Cuts at: 4002 6582 12381 4002 Size: 2580 5799 4339 Fragments arranged by size: 5799 4339 2580 BspHI T'CATG_A Cuts at: 4114 5967 9013 9399 10607 12337 4114 Size: 1853 3046 386 1208 1730 4495 Fragments arranged by size: 4495 3046 1853 1730 1208 386 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2779 3269 3402 4329 4980 5275 5637 5760 Size: 1935 490 133 927 651 295 362 123 Cuts at: 5760 5877 5978 6045 6123 7290 7674 8112 8220 Size: 117 101 67 78 1167 384 438 108 Cuts at: 8220 9284 9579 10073 844 Size: 1064 295 494 3489 Fragments arranged by size: 3489 1935 1167 1064 927 651 494 490 438 384 362 295 295 133 123 117 108 101 78 67 BsrBI CCG'CTC Cuts at: 1394 1823 3235 6817 9105 9361 10605 1394 Size: 429 1412 3582 2288 256 1244 3507 Fragments arranged by size: 3582 3507 2288 1412 1244 429 256 BsrDI GCAATG_nn' Cuts at: 65 1248 3091 3723 8658 8723 9103 9847 11213 Size: 1183 1843 632 4935 65 380 744 1366 Cuts at: 11213 11387 65 Size: 174 1396 Fragments arranged by size: 4935 1843 1396 1366 1183 744 632 380 174 65 BsrFI r'CCGG_y Cuts at: 1700 1810 2618 4154 4390 5349 7182 8022 8221 Size: 110 808 1536 236 959 1833 840 199 Cuts at: 8221 8463 9204 11362 11549 1700 Size: 242 741 2158 187 2869 Fragments arranged by size: 2869 2158 1833 1536 959 840 808 741 242 236 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 12718 BssHII G'CGCG_C Cuts at: 1925 4324 1925 Size: 2399 10319 BssSI C'ACGA_G Cuts at: 4043 7559 7889 10778 11790 4043 Size: 3516 330 2889 1012 4971 Fragments arranged by size: 4971 3516 2889 1012 330 BstAPI GCAn_nnn'nTGC Cuts at: 794 2281 2584 5590 5863 6508 7018 7021 8212 Size: 1487 303 3006 273 645 510 3 1191 Cuts at: 8212 794 Size: 5300 Fragments arranged by size: 5300 3006 1487 1191 645 510 303 273 3 BstBI TT'CG_AA Cuts at: 11522 11522 Size: 12718 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 12718 BstXI CCAn_nnnn'nTGG Cuts at: 3370 3895 4504 5668 6634 7210 8950 8998 9238 Size: 525 609 1164 966 576 1740 48 240 Cuts at: 9238 9907 3370 Size: 669 6181 Fragments arranged by size: 6181 1740 1164 966 669 609 576 525 240 48 BstYI r'GATC_y Cuts at: 1972 2989 3318 4254 4419 4914 5637 5673 6294 Size: 1017 329 936 165 495 723 36 621 Cuts at: 6294 7248 7305 7963 8736 10048 10415 10800 10817 Size: 954 57 658 773 1312 367 385 17 Cuts at: 10817 12269 12355 12367 12713 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1312 1017 954 936 773 723 658 621 495 385 367 346 329 165 86 57 36 17 12 Bsu36I CC'TnA_GG Cuts at: 9321 9321 Size: 12718 BtgI C'CryG_G Cuts at: 513 1065 2124 2884 3369 4503 4782 5361 5457 Size: 552 1059 760 485 1134 279 579 96 Cuts at: 5457 6539 6567 6594 6627 7410 7758 8914 8943 Size: 1082 28 27 33 783 348 1156 29 Cuts at: 8943 9406 513 Size: 463 3825 Fragments arranged by size: 3825 1156 1134 1082 1059 783 760 579 552 485 463 348 279 96 33 29 28 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3076 3471 3916 6009 8740 487 Size: 683 56 1850 395 445 2093 2731 4465 Fragments arranged by size: 4465 2731 2093 1850 683 445 395 56 BtsI GCAGTG_nn' Cuts at: 2368 2807 3789 4664 5061 5407 5506 6259 6670 Size: 439 982 875 397 346 99 753 411 Cuts at: 6670 6904 7591 9286 9921 10234 11028 11048 2368 Size: 234 687 1695 635 313 794 20 4038 Fragments arranged by size: 4038 1695 982 875 794 753 687 635 439 411 397 346 313 234 99 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12718 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 9406 9441 529 Size: 35 8842 35 3806 Fragments arranged by size: 8842 3806 35 35 DraI TTT'AAA Cuts at: 10022 10022 Size: 12718 DraIII CAC_nnn'GTG Cuts at: 5863 7179 7564 5863 Size: 1316 385 11017 Fragments arranged by size: 11017 1316 385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2534 3494 5999 7891 11725 809 Size: 507 1218 960 2505 1892 3834 1802 Fragments arranged by size: 3834 2505 1892 1802 1218 960 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2829 3360 Size: 54 1425 50 275 18 297 702 531 Cuts at: 3360 3774 4242 4701 5158 5874 6120 6600 7272 Size: 414 468 459 457 716 246 480 672 Cuts at: 7272 8637 9100 10165 11054 12683 8 Size: 1365 463 1065 889 1629 43 Fragments arranged by size: 1629 1425 1365 1065 889 716 702 672 531 480 468 463 459 457 414 297 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2127 5158 10165 1537 Size: 275 315 3031 5007 4090 Fragments arranged by size: 5007 4090 3031 315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 2381 2495 4065 4115 5243 5765 6890 Size: 824 204 114 1570 50 1128 522 1125 Cuts at: 6890 8109 8266 8474 8735 9155 9176 9327 9476 Size: 1219 157 208 261 420 21 151 149 Cuts at: 9476 10648 1353 Size: 1172 3423 Fragments arranged by size: 3423 1570 1219 1172 1128 1125 824 522 420 261 208 204 157 151 149 114 50 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3653 4937 5114 8777 11305 11679 Size: 1064 138 2064 1284 177 3663 2528 374 Cuts at: 11679 11825 387 Size: 146 1280 Fragments arranged by size: 3663 2528 2064 1284 1280 1064 374 177 146 138 EcoICRI GAG'CTC Cuts at: 2605 3955 9940 2605 Size: 1350 5985 5383 Fragments arranged by size: 5985 5383 1350 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2717 3590 3638 4223 4621 4795 Size: 393 821 264 873 48 585 398 174 Cuts at: 4795 4913 4931 5159 5233 5615 5801 6203 6427 Size: 118 18 228 74 382 186 402 224 Cuts at: 6427 6688 6830 6842 6911 7073 8170 8237 9653 Size: 261 142 12 69 162 1097 67 1416 Cuts at: 9653 9707 9863 10781 11378 12165 12433 1239 Size: 54 156 918 597 787 268 1524 Fragments arranged by size: 1524 1416 1097 918 873 821 787 597 585 402 398 393 382 268 264 261 228 224 186 174 162 156 142 118 74 69 67 54 48 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 2174 2699 3551 3776 6833 8108 8447 9332 2174 Size: 525 852 225 3057 1275 339 885 5560 Fragments arranged by size: 5560 3057 1275 885 852 525 339 225 EcoO109I rG'GnC_Cy Cuts at: 2134 3574 3602 4694 4695 4723 5734 6961 7117 Size: 1440 28 1092 1 28 1011 1227 156 Cuts at: 7117 7538 7663 7852 7853 9325 9758 2134 Size: 421 125 189 1 1472 433 5094 Fragments arranged by size: 5094 1472 1440 1227 1092 1011 433 421 189 156 125 28 28 1 1 EcoRI G'AATT_C Cuts at: 8960 10027 10405 8960 Size: 1067 378 11273 Fragments arranged by size: 11273 1067 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3304 9202 3304 Size: 5898 6820 FspI TGC'GCA Cuts at: 3872 7064 11224 3872 Size: 3192 4160 5366 Fragments arranged by size: 5366 4160 3192 HaeII r_GCGC'y Cuts at: 1678 5260 5433 5890 6916 7357 8177 8318 8779 Size: 3582 173 457 1026 441 820 141 461 Cuts at: 8779 8807 11865 1678 Size: 28 3058 2531 Fragments arranged by size: 3582 3058 2531 1026 820 461 457 441 173 141 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2545 2577 3080 Size: 32 135 32 458 32 483 32 503 Cuts at: 3080 3112 3335 3367 3611 3643 3890 3922 4241 Size: 32 223 32 244 32 247 32 319 Cuts at: 4241 4273 6008 6040 6179 6211 7331 7363 7391 Size: 32 1735 32 139 32 1120 32 28 Cuts at: 7391 7423 7976 8008 8513 8545 8675 8707 9287 Size: 32 553 32 505 32 130 32 580 Cuts at: 9287 9319 11359 11391 11433 11465 1373 Size: 32 2040 32 42 32 2626 Fragments arranged by size: 2626 2040 1735 1120 580 553 505 503 483 458 319 247 244 223 139 135 130 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 7423 7471 9202 10062 10308 669 Size: 988 281 5485 48 1731 860 246 3079 Fragments arranged by size: 5485 3079 1731 988 860 281 246 48 HindIII A'AGCT_T Cuts at: 748 748 Size: 12718 HpaI GTT'AAC Cuts at: 10308 10308 Size: 12718 KasI G'GCGC_C Cuts at: 5429 5886 5429 Size: 457 12261 KpnI G_GTAC'C Cuts at: 4198 10043 4198 Size: 5845 6873 MfeI C'AATT_G Cuts at: 10317 10317 Size: 12718 MluI A'CGCG_T Cuts at: 4464 12594 4464 Size: 8130 4588 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3948 4479 5319 6954 11832 12016 1305 Size: 2643 531 840 1635 4878 184 2007 Fragments arranged by size: 4878 2643 2007 1635 840 531 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3362 4244 5876 6602 7274 Size: 54 1425 343 1530 882 1632 726 672 Cuts at: 7274 10 Size: 5454 Fragments arranged by size: 5454 1632 1530 1425 882 726 672 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 2450 2904 3084 3209 4784 4892 Size: 648 1177 107 454 180 125 1575 108 Cuts at: 4892 5202 5966 6227 6278 6995 7601 8031 8699 Size: 310 764 261 51 717 606 430 668 Cuts at: 8699 8948 8954 9398 9524 10676 11035 11194 518 Size: 249 6 444 126 1152 359 159 2042 Fragments arranged by size: 2042 1575 1177 1152 764 717 668 648 606 454 444 430 359 310 261 249 180 159 126 125 108 107 51 6 MspA1I CmG'CkG Cuts at: 1606 2109 2126 2658 3427 4135 4201 4267 4855 Size: 503 17 532 769 708 66 66 588 Cuts at: 4855 5212 5233 5293 6181 6505 6675 6683 6694 Size: 357 21 60 888 324 170 8 11 Cuts at: 6694 7060 7639 8203 8290 8581 9964 10134 10811 Size: 366 579 564 87 291 1383 170 677 Cuts at: 10811 11959 12204 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1383 1148 888 769 708 677 588 579 564 532 503 366 357 324 291 245 170 170 87 66 66 60 21 17 11 8 NaeI GCC'GGC Cuts at: 2620 4156 7184 8024 2620 Size: 1536 3028 840 7314 Fragments arranged by size: 7314 3028 1536 840 NarI GG'CG_CC Cuts at: 5430 5887 5430 Size: 457 12261 NcoI C'CATG_G Cuts at: 513 1065 4503 4782 5361 6594 6627 8943 9406 Size: 552 3438 279 579 1233 33 2316 463 Cuts at: 9406 513 Size: 3825 Fragments arranged by size: 3825 3438 2316 1233 579 552 463 279 33 NdeI CA'TA_TG Cuts at: 387 387 Size: 12718 NgoMIV G'CCGG_C Cuts at: 2618 4154 7182 8022 2618 Size: 1536 3028 840 7314 Fragments arranged by size: 7314 3028 1536 840 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12718 NotI GC'GGCC_GC Cuts at: 5158 10165 5158 Size: 5007 7711 NspI r_CATG'y Cuts at: 1132 1229 2291 2591 3661 4222 5137 5167 8578 Size: 97 1062 300 1070 561 915 30 3411 Cuts at: 8578 9618 9700 10076 11621 1132 Size: 1040 82 376 1545 2229 Fragments arranged by size: 3411 2229 1545 1070 1062 1040 915 561 376 300 97 82 30 PasI CC'CwG_GG Cuts at: 1348 9238 1348 Size: 7890 4828 PciI A'CATG_T Cuts at: 3657 9696 11617 3657 Size: 6039 1921 4758 Fragments arranged by size: 6039 4758 1921 PflMI CCAn_nnn'nTGG Cuts at: 1108 3192 3430 4240 4267 4381 4762 6040 6759 Size: 2084 238 810 27 114 381 1278 719 Cuts at: 6759 7285 7825 8077 1108 Size: 526 540 252 5749 Fragments arranged by size: 5749 2084 1278 810 719 540 526 381 252 238 114 27 PfoI T'CCnGG_A Cuts at: 3483 3819 3894 4917 5529 5778 6888 7254 7734 Size: 336 75 1023 612 249 1110 366 480 Cuts at: 7734 9327 9879 3483 Size: 1593 552 6322 Fragments arranged by size: 6322 1593 1110 1023 612 552 480 366 336 249 75 PmeI GTTT'AAAC Cuts at: 10022 10022 Size: 12718 PmlI CAC'GTG Cuts at: 3192 4089 4354 4360 8144 8974 3192 Size: 897 265 6 3784 830 6936 Fragments arranged by size: 6936 3784 897 830 265 6 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2183 2215 3875 3907 9053 9085 10787 10819 12300 Size: 32 1660 32 5146 32 1702 32 1481 Cuts at: 12300 12332 2183 Size: 32 2569 Fragments arranged by size: 5146 2569 1702 1660 1481 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 3602 4723 6961 7117 7663 9325 2134 Size: 1468 1121 2238 156 546 1662 5527 Fragments arranged by size: 5527 2238 1662 1468 1121 546 156 PshAI GACnn'nnGTC Cuts at: 1613 3857 8993 1613 Size: 2244 5136 5338 Fragments arranged by size: 5338 5136 2244 PsiI TTA'TAA Cuts at: 10288 10288 Size: 12718 PspOMI G'GGCC_C Cuts at: 4694 7389 7537 7852 8840 9757 4694 Size: 2695 148 315 988 917 7655 Fragments arranged by size: 7655 2695 988 917 315 148 PspXI vC'TCGA_Gb Cuts at: 6530 6530 Size: 12718 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2954 2986 3632 3664 6248 6280 9695 9727 2954 Size: 32 646 32 2584 32 3415 32 5945 Fragments arranged by size: 5945 3415 2584 646 32 32 32 32 PstI C_TGCA'G Cuts at: 830 2277 2286 2790 4713 5232 5637 5703 6362 Size: 1447 9 504 1923 519 405 66 659 Cuts at: 6362 6480 6513 6792 7014 7638 7671 7731 7977 Size: 118 33 279 222 624 33 60 246 Cuts at: 7977 8109 8181 8208 9281 10070 830 Size: 132 72 27 1073 789 3478 Fragments arranged by size: 3478 1923 1447 1073 789 659 624 519 504 405 279 246 222 132 118 72 66 60 33 33 27 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12718 PvuII CAG'CTG Cuts at: 2658 3427 4135 4201 4267 5233 5293 6505 6675 Size: 769 708 66 66 966 60 1212 170 Cuts at: 6675 6683 7060 7639 8203 8290 2658 Size: 8 377 579 564 87 7086 Fragments arranged by size: 7086 1212 966 769 708 579 564 377 170 87 66 66 60 8 RsrII CG'GwC_CG Cuts at: 4005 4005 Size: 12718 SacI G_AGCT'C Cuts at: 2607 3957 9942 2607 Size: 1350 5985 5383 Fragments arranged by size: 5985 5383 1350 SacII CC_GC'GG Cuts at: 2127 2127 Size: 12718 SalI G'TCGA_C Cuts at: 1936 7469 9200 10060 1936 Size: 5533 1731 860 4594 Fragments arranged by size: 5533 4594 1731 860 SapI GCTCTTCn'nnn_ Cuts at: 2381 2495 5243 6890 8109 8474 8735 2381 Size: 114 2748 1647 1219 365 261 6364 Fragments arranged by size: 6364 2748 1647 1219 365 261 114 SbfI CC_TGCA'GG Cuts at: 8109 10070 8109 Size: 1961 10757 ScaI AGT'ACT Cuts at: 2216 3059 4064 4238 7844 10966 12678 2216 Size: 843 1005 174 3606 3122 1712 2256 Fragments arranged by size: 3606 3122 2256 1712 1005 843 174 SexAI A'CCwGG_T Cuts at: 3438 3438 Size: 12718 SfcI C'TryA_G Cuts at: 826 1045 2273 2282 2786 2934 4189 4630 4709 Size: 219 1228 9 504 148 1255 441 79 Cuts at: 4709 5228 5633 5699 6358 6476 6509 6545 6788 Size: 519 405 66 659 118 33 36 243 Cuts at: 6788 7010 7634 7667 7727 7973 8105 8177 8204 Size: 222 624 33 60 246 132 72 27 Cuts at: 8204 8779 9025 9277 10066 11201 11882 12073 826 Size: 575 246 252 789 1135 681 191 1471 Fragments arranged by size: 1471 1255 1228 1135 789 681 659 624 575 519 504 441 405 252 246 246 243 222 219 191 148 132 118 79 72 66 60 36 33 33 27 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3027 3027 Size: 12718 SfoI GGC'GCC Cuts at: 5431 5888 5431 Size: 457 12261 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12718 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12718 SmaI CCC'GGG Cuts at: 1872 10045 1872 Size: 8173 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6530 7025 7979 8303 9176 9194 Size: 197 926 4587 495 954 324 873 18 Cuts at: 9194 9605 9941 10822 11723 11985 820 Size: 411 336 881 901 262 1553 Fragments arranged by size: 4587 1553 954 926 901 881 873 495 411 336 324 262 197 18 SnaBI TAC'GTA Cuts at: 493 10509 493 Size: 10016 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12718 SphI G_CATG'C Cuts at: 1132 2291 2591 5137 8578 10076 1132 Size: 1159 300 2546 3441 1498 3774 Fragments arranged by size: 3774 3441 2546 1498 1159 300 SspI AAT'ATT Cuts at: 5 52 10642 5 Size: 47 10590 2081 Fragments arranged by size: 10590 2081 47 StuI AGG'CCT Cuts at: 1517 3287 4715 6575 6905 8099 1517 Size: 1770 1428 1860 330 1194 6136 Fragments arranged by size: 6136 1860 1770 1428 1194 330 StyI C'CwwG_G Cuts at: 513 1065 2994 3270 4503 4782 5361 5436 6393 Size: 552 1929 276 1233 279 579 75 957 Cuts at: 6393 6594 6603 6627 6957 7824 8943 9406 9982 Size: 201 9 24 330 867 1119 463 576 Cuts at: 9982 10157 513 Size: 175 3074 Fragments arranged by size: 3074 1929 1233 1119 957 867 579 576 552 463 330 279 276 201 175 75 24 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3857 3962 4022 5374 5496 7190 8849 Size: 1255 2277 105 60 1352 122 1694 1659 Cuts at: 8849 9761 10745 10762 10915 11100 11545 325 Size: 912 984 17 153 185 445 1498 Fragments arranged by size: 2277 1694 1659 1498 1352 1255 984 912 445 185 153 122 105 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 2772 3057 Size: 274 80 33 51 1443 237 558 285 Cuts at: 3057 4062 4236 7842 9711 10964 12676 96 Size: 1005 174 3606 1869 1253 1712 138 Fragments arranged by size: 3606 1869 1712 1443 1253 1005 558 285 274 237 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2709 2741 4760 4792 6548 6580 6971 7003 7730 Size: 32 2019 32 1756 32 391 32 727 Cuts at: 7730 7762 8838 8870 12678 12710 2709 Size: 32 1076 32 3808 32 2717 Fragments arranged by size: 3808 2717 2019 1756 1076 727 391 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2042 7405 9299 9661 1316 Size: 726 5363 1894 362 4373 Fragments arranged by size: 5363 4373 1894 726 362 XbaI T'CTAG_A Cuts at: 10054 10054 Size: 12718 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3355 3844 4510 4759 5074 5113 6964 7282 7286 Size: 489 666 249 315 39 1851 318 4 Cuts at: 7286 7411 7489 7708 7765 8002 8701 8827 9904 Size: 125 78 219 57 237 699 126 1077 Cuts at: 9904 3355 Size: 6169 Fragments arranged by size: 6169 1851 1077 699 666 489 318 315 249 237 219 126 125 78 57 39 4 XhoI C'TCGA_G Cuts at: 1943 6530 9194 1943 Size: 4587 2664 5467 Fragments arranged by size: 5467 4587 2664 XmaI C'CCGG_G Cuts at: 1870 10043 1870 Size: 8173 4545 XmnI GAAnn'nnTTC Cuts at: 4926 7250 7316 10847 4926 Size: 2324 66 3531 6797 Fragments arranged by size: 6797 3531 2324 66 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8086 10526 276 Size: 53 83 186 936 6552 2440 2468 Fragments arranged by size: 6552 2468 2440 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI SanDI SrfI SwaI