Restriction map for
pFN21AE3826
(Linear) (Six-base) MAPSORT of: KIEE3826.seq Check: 9188 from: 1 to: 8013
KIEE3826.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 774 1264 3755 3872 3973 4040 5285 6215
Size: 774 490 2491 117 101 67 1245 930
Cuts at: 6215 7279 8013
Size: 1064 734
Fragments arranged by size:
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101 67
AatII G_ACGT'C
Cuts at: 0 6083 8013
Size: 6083 1930
AccI GT'mk_AC
Cuts at: 0 4142 4510 5465 7139 7196 8013
Size: 4142 368 955 1674 57 817
Fragments arranged by size:
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Acc65I G'GTAC_C
Cuts at: 0 2189 8013
Size: 2189 5824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 2218 2616 2790 2908 2926 3228 3610
Size: 1585 633 398 174 118 18 302 382
Cuts at: 3610 4422 4837 6232 8013
Size: 812 415 1395 1781
Fragments arranged by size:
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302 174 118 18
AfeI AGC'GCT
Cuts at: 0 4909 5350 6772 8013
Size: 4909 441 1422 1241
Fragments arranged by size:
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AflII C'TTAA_G
Cuts at: 0 7600 8013
Size: 7600 413
AflIII A'CryG_T
Cuts at: 0 757 1184 1652 2459 5568 6359 7691 8013
Size: 757 427 468 807 3109 791 1332 322
Fragments arranged by size:
3109 1332 807 791 757 468 427 322
AhdI GACnn_n'nnGTC
Cuts at: 0 4188 5340 8013
Size: 4188 1152 2673
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 1204 2779 4222 4273 5596 6694 8013
Size: 1204 1575 1443 51 1323 1098 1319
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1222 1981 2752 3181 3215 4183 4486 5008
Size: 1222 759 771 429 34 968 303 522
Cuts at: 5008 5071 5083 5614 8013
Size: 63 12 531 2399
Fragments arranged by size:
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303 63 34 12
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1870 1902 4450 4482 5080 5112 6346 6378
Size: 1870 32 2548 32 598 32 1234 32
Cuts at: 6378 7048 7080 8013
Size: 670 32 933
Fragments arranged by size:
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32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 276 372 579 1425 1521 2262 2376 3040
Size: 276 96 207 846 96 741 114 664
Cuts at: 3040 3291 3693 3732 3897 4503 5016 5637 5730
Size: 251 402 39 165 606 513 621 93
Cuts at: 5730 6072 6207 6438 8013
Size: 342 135 231 1575
Fragments arranged by size:
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342 276 251 231 207 165 135 114
96 96 93 39
ApaI G_GGCC'C
Cuts at: 0 2693 5388 5536 5851 6839 7756 8013
Size: 2693 2695 148 315 988 917 257
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 2350 3859 4158 8013
Size: 2350 1509 299 3855
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 6955 8013
Size: 6955 1058
AvaI C'yCGr_G
Cuts at: 0 2465 3213 4346 4525 7189 7475 8013
Size: 2465 748 1133 179 2664 286 538
Fragments arranged by size:
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AvrII C'CTAG_G
Cuts at: 0 7977 8013
Size: 7977 36
BamHI G'GATC_C
Cuts at: 0 6731 8013
Size: 6731 1282
BanI G'GyrC_C
Cuts at: 0 1169 1252 2189 3217 3424 3881 6856 6913
Size: 1169 83 937 1028 207 457 2975 57
Cuts at: 6913 7311 8013
Size: 398 702
Fragments arranged by size:
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83 57
BanII G_rGCy'C
Cuts at: 0 61 119 602 1883 1952 2693 3773 4561
Size: 61 58 483 1281 69 741 1080 788
Cuts at: 4561 5388 5536 5780 5851 6136 6839 7307 7756
Size: 827 148 244 71 285 703 468 449
Cuts at: 7756 7937 8013
Size: 181 76
Fragments arranged by size:
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449 285 244 181 148 76 71 69
61 58
BbeI G_GCGC'C
Cuts at: 0 3428 3885 8013
Size: 3428 457 4128
Fragments arranged by size:
4128 3428 457
BbsI GAAGACnn'nnnn_
Cuts at: 0 1436 2796 2970 3124 5032 6338 8013
Size: 1436 1360 174 154 1908 1306 1675
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 950 6306 8013
Size: 950 5356 1707
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1068 1102 4476 4510 8013
Size: 1068 34 3374 34 3503
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 3326 3959 7778 8013
Size: 3326 633 3819 235
Fragments arranged by size:
3819 3326 633 235
BglI GCCn_nnn'nGGC
Cuts at: 0 823 1022 1239 2140 4594 7123 8013
Size: 823 199 217 901 2454 2529 890
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 2249 4289 5243 8013
Size: 2249 2040 954 2770
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 601 2176 2785 2843 4405 4654 4835 6038
Size: 601 1575 609 58 1562 249 181 1203
Cuts at: 6038 6265 8013
Size: 227 1748
Fragments arranged by size:
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181 58
Bme1580I G_kGCm'C
Cuts at: 0 181 896 1011 1145 1211 1255 1304 2354
Size: 181 715 115 134 66 44 49 1050
Cuts at: 2354 2549 2693 2765 2814 3222 3863 4162 5388
Size: 195 144 72 49 408 641 299 1226
Cuts at: 5388 5536 5555 5593 5851 6422 6839 6861 7756
Size: 148 19 38 258 571 417 22 895
Cuts at: 7756 8013
Size: 257
Fragments arranged by size:
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299 258 257 195 181 148 144 134
115 72 66 49 49 44 38 22
19
BmgBI CAC'GTC
Cuts at: 0 717 758 3309 4185 6362 8013
Size: 717 41 2551 876 2177 1651
Fragments arranged by size:
2551 2177 1651 876 717 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 956 5166 6409 8013
Size: 956 4210 1243 1604
Fragments arranged by size:
4210 1604 1243 956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6270 8013
Size: 6270 1743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 712 1633 3154 3796 4198 4683 4825
Size: 448 264 921 1521 642 402 485 142
Cuts at: 4825 4906 5068 6165 7648 7702 7858 8013
Size: 81 162 1097 1483 54 156 155
Fragments arranged by size:
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264 162 156 155 142 81 54
Bpu10I CC'TnA_GC
Cuts at: 0 950 2047 4871 6306 8013
Size: 950 1097 2824 1435 1707
Fragments arranged by size:
2824 1707 1435 1097 950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5005 5959 6283 7156 7921 8013
Size: 5005 954 324 873 765 92
Fragments arranged by size:
5005 954 873 765 324 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 1313 1840 2890 3216 3778 5389 5818 8013
Size: 1313 527 1050 326 562 1611 429 2195
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 1187 2084 2349 2355 4263 4401 6139 6969
Size: 1187 897 265 6 1908 138 1738 830
Cuts at: 6969 7767 8013
Size: 798 246
Fragments arranged by size:
1908 1738 1187 897 830 798 265 246
138 6
BsaBI GATnn'nnATC
Cuts at: 0 3667 6736 7381 8013
Size: 3667 3069 645 632
Fragments arranged by size:
3667 3069 645 632
BsaHI Gr'CG_yC
Cuts at: 0 1340 3425 3882 6080 8013
Size: 1340 2085 457 2198 1933
Fragments arranged by size:
2198 2085 1933 1340 457
BsaWI w'CCGG_w
Cuts at: 0 1997 4577 5090 8013
Size: 1997 2580 513 2923
Fragments arranged by size:
2923 2580 1997 513
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 55 685 704 715 734 1330 1360
Size: 25 30 630 19 11 19 596 30
Cuts at: 1360 1606 1636 1870 1900 2236 2266 3088 3118
Size: 246 30 234 30 336 30 822 30
Cuts at: 3118 4543 4573 4966 4996 5386 5416 5971 6001
Size: 1425 30 393 30 390 30 555 30
Cuts at: 6001 6508 6538 6574 6604 6670 6700 7048 7078
Size: 507 30 36 30 66 30 348 30
Cuts at: 7078 7132 7162 7201 7231 8013
Size: 54 30 39 30 782
Fragments arranged by size:
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390 348 336 246 234 66 54 39
36 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 25 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 709 899 967 1237 1240 1630 2575 2884
Size: 709 190 68 270 3 390 945 309
Cuts at: 2884 3424 3475 4513 4822 4987 5502 6250 6253
Size: 540 51 1038 309 165 515 748 3
Cuts at: 6253 6337 6340 7069 7606 8013
Size: 84 3 729 537 407
Fragments arranged by size:
1038 945 748 729 709 540 537 515
407 390 309 309 270 190 165 84
68 51 3 3 3
BseYI C'CCAG_C
Cuts at: 0 889 1036 1248 2255 2262 2664 2815 2827
Size: 889 147 212 1007 7 402 151 12
Cuts at: 2827 4685 5387 5529 5536 5772 7470 7922 8013
Size: 1858 702 142 7 236 1698 452 91
Fragments arranged by size:
1858 1698 1007 889 702 452 402 236
212 151 147 142 91 12 7 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1161 1780 2329 2704 3457 3721 3728
Size: 358 803 619 549 375 753 264 7
Cuts at: 3728 3733 3892 3910 4516 4694 4846 5350 5593
Size: 5 159 18 606 178 152 504 243
Cuts at: 5593 6517 8013
Size: 924 1496
Fragments arranged by size:
1496 924 803 753 619 606 549 504
375 358 264 243 178 159 152 18
7 5
BsiEI CG_ry'CG
Cuts at: 0 125 3156 5373 7200 8013
Size: 125 3031 2217 1827 813
Fragments arranged by size:
3031 2217 1827 813 125
BsiHKAI G_wGCw'C
Cuts at: 0 602 1201 1718 1952 2177 2354 3176 3863
Size: 602 599 517 234 225 177 822 687
Cuts at: 3863 4162 5603 7688 7937 8013
Size: 299 1441 2085 249 76
Fragments arranged by size:
2085 1441 822 687 602 599 517 299
249 234 225 177 76
BsiWI C'GTAC_G
Cuts at: 0 1154 8013
Size: 1154 6859
BsmI GAATG_Cn'
Cuts at: 0 1038 1559 1683 6227 8013
Size: 1038 521 124 4544 1786
Fragments arranged by size:
4544 1786 1038 521 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 45 707 2407 5710 7051 8013
Size: 45 662 1700 3303 1341 962
Fragments arranged by size:
3303 1700 1341 962 662 45
Bsp1286I G_dGCh'C
Cuts at: 0 61 119 181 602 896 1011 1145 1201
Size: 61 58 62 421 294 115 134 56
Cuts at: 1201 1211 1255 1304 1718 1883 1952 2177 2354
Size: 10 44 49 414 165 69 225 177
Cuts at: 2354 2549 2693 2765 2814 3176 3222 3773 3863
Size: 195 144 72 49 362 46 551 90
Cuts at: 3863 4162 4561 5388 5536 5555 5593 5603 5780
Size: 299 399 827 148 19 38 10 177
Cuts at: 5780 5851 6136 6422 6839 6861 7307 7688 7756
Size: 71 285 286 417 22 446 381 68
Cuts at: 7756 7937 8013
Size: 181 76
Fragments arranged by size:
827 551 446 421 417 414 399 381
362 299 294 286 285 225 195 181
177 177 165 148 144 134 115 90
76 72 71 69 68 62 61 58
56 49 49 46 44 38 22 19
10 10
BspEI T'CCGG_A
Cuts at: 0 1997 4577 8013
Size: 1997 2580 3436
Fragments arranged by size:
3436 2580 1997
BspHI T'CATG_A
Cuts at: 0 2109 3962 7008 7394 8013
Size: 2109 1853 3046 386 619
Fragments arranged by size:
3046 2109 1853 619 386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 774 1264 1397 2324 2975 3270 3632 3755
Size: 774 490 133 927 651 295 362 123
Cuts at: 3755 3872 3973 4040 4118 5285 5669 6107 6215
Size: 117 101 67 78 1167 384 438 108
Cuts at: 6215 7279 7574 8013
Size: 1064 295 439
Fragments arranged by size:
1167 1064 927 774 651 490 439 438
384 362 295 295 133 123 117 108
101 78 67
BsrBI CCG'CTC
Cuts at: 0 1230 4812 7100 7356 8013
Size: 1230 3582 2288 256 657
Fragments arranged by size:
3582 2288 1230 657 256
BsrDI GCAATG_nn'
Cuts at: 0 1086 1718 6653 6718 7098 7842 8013
Size: 1086 632 4935 65 380 744 171
Fragments arranged by size:
4935 1086 744 632 380 171 65
BsrFI r'CCGG_y
Cuts at: 0 613 2149 2385 3344 5177 6017 6216 6458
Size: 613 1536 236 959 1833 840 199 242
Cuts at: 6458 7199 8013
Size: 741 814
Fragments arranged by size:
1833 1536 959 840 814 741 613 242
236 199
BssHII G'CGCG_C
Cuts at: 0 2319 8013
Size: 2319 5694
BssSI C'ACGA_G
Cuts at: 0 2038 5554 5884 8013
Size: 2038 3516 330 2129
Fragments arranged by size:
3516 2129 2038 330
BstAPI GCAn_nnn'nTGC
Cuts at: 0 276 579 3585 3858 4503 5013 5016 6207
Size: 276 303 3006 273 645 510 3 1191
Cuts at: 6207 8013
Size: 1806
Fragments arranged by size:
3006 1806 1191 645 510 303 276 273
3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1365 1890 2499 3663 4629 5205 6945 6993
Size: 1365 525 609 1164 966 576 1740 48
Cuts at: 6993 7233 7902 8013
Size: 240 669 111
Fragments arranged by size:
1740 1365 1164 966 669 609 576 525
240 111 48
BstYI r'GATC_y
Cuts at: 0 984 1313 2249 2414 2909 3632 3668 4289
Size: 984 329 936 165 495 723 36 621
Cuts at: 4289 5243 5300 5958 6731 8013
Size: 954 57 658 773 1282
Fragments arranged by size:
1282 984 954 936 773 723 658 621
495 329 165 57 36
Bsu36I CC'TnA_GG
Cuts at: 0 7316 8013
Size: 7316 697
BtgI C'CryG_G
Cuts at: 0 119 879 1364 2498 2777 3356 3452 4534
Size: 119 760 485 1134 279 579 96 1082
Cuts at: 4534 4562 4589 4622 5405 5753 6909 6938 7401
Size: 28 27 33 783 348 1156 29 463
Cuts at: 7401 8013
Size: 612
Fragments arranged by size:
1156 1134 1082 783 760 612 579 485
463 348 279 119 96 33 29 28
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1071 1466 1911 4004 6735 8013
Size: 1071 395 445 2093 2731 1278
Fragments arranged by size:
2731 2093 1278 1071 445 395
BtsI GCAGTG_nn'
Cuts at: 0 363 802 1784 2659 3056 3402 3501 4254
Size: 363 439 982 875 397 346 99 753
Cuts at: 4254 4665 4899 5586 7281 7916 8013
Size: 411 234 687 1695 635 97
Fragments arranged by size:
1695 982 875 753 687 635 439 411
397 363 346 234 99 97
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 7401 7436 8013
Size: 7401 35 577
Fragments arranged by size:
7401 577 35
DraIII CAC_nnn'GTG
Cuts at: 0 3858 5174 5559 8013
Size: 3858 1316 385 2454
Fragments arranged by size:
3858 2454 1316 385
DrdI GACnn_nn'nnGTC
Cuts at: 0 529 1489 3994 5886 8013
Size: 529 960 2505 1892 2127
Fragments arranged by size:
2505 2127 1892 960 529
EaeI y'GGCC_r
Cuts at: 0 122 824 1355 1769 2237 2696 3153 3869
Size: 122 702 531 414 468 459 457 716
Cuts at: 3869 4115 4595 5267 6632 7095 8013
Size: 246 480 672 1365 463 918
Fragments arranged by size:
1365 918 716 702 672 531 480 468
463 459 457 414 246 122
EagI C'GGCC_G
Cuts at: 0 122 3153 8013
Size: 122 3031 4860
Fragments arranged by size:
4860 3031 122
EarI CTCTTCn'nnn_
Cuts at: 0 172 376 490 2060 2110 3238 3760 4885
Size: 172 204 114 1570 50 1128 522 1125
Cuts at: 4885 6104 6261 6469 6730 7150 7171 7322 7471
Size: 1219 157 208 261 420 21 151 149
Cuts at: 7471 8013
Size: 542
Fragments arranged by size:
1570 1219 1128 1125 542 522 420 261
208 204 172 157 151 149 114 50
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1648 2932 3109 6772 8013
Size: 1648 1284 177 3663 1241
Fragments arranged by size:
3663 1648 1284 1241 177
EcoICRI GAG'CTC
Cuts at: 0 600 1950 7935 8013
Size: 600 1350 5985 78
Fragments arranged by size:
5985 1350 600 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 712 1585 1633 2218 2616 2790 2908
Size: 448 264 873 48 585 398 174 118
Cuts at: 2908 2926 3154 3228 3610 3796 4198 4422 4683
Size: 18 228 74 382 186 402 224 261
Cuts at: 4683 4825 4837 4906 5068 6165 6232 7648 7702
Size: 142 12 69 162 1097 67 1416 54
Cuts at: 7702 7858 8013
Size: 156 155
Fragments arranged by size:
1416 1097 873 585 448 402 398 382
264 261 228 224 186 174 162 156
155 142 118 74 69 67 54 48
18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 169 694 1546 1771 4828 6103 6442 7327
Size: 169 525 852 225 3057 1275 339 885
Cuts at: 7327 8013
Size: 686
Fragments arranged by size:
3057 1275 885 852 686 525 339 225
169
EcoO109I rG'GnC_Cy
Cuts at: 0 129 1569 1597 2689 2690 2718 3729 4956
Size: 129 1440 28 1092 1 28 1011 1227
Cuts at: 4956 5112 5533 5658 5847 5848 7320 7753 8013
Size: 156 421 125 189 1 1472 433 260
Fragments arranged by size:
1472 1440 1227 1092 1011 433 421 260
189 156 129 125 28 28 1 1
EcoRI G'AATT_C
Cuts at: 0 6955 8013
Size: 6955 1058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1299 7197 8013
Size: 1299 5898 816
Fragments arranged by size:
5898 1299 816
FspI TGC'GCA
Cuts at: 0 1867 5059 8013
Size: 1867 3192 2954
Fragments arranged by size:
3192 2954 1867
HaeII r_GCGC'y
Cuts at: 0 3255 3428 3885 4911 5352 6172 6313 6774
Size: 3255 173 457 1026 441 820 141 461
Cuts at: 6774 6802 8013
Size: 28 1211
Fragments arranged by size:
3255 1211 1026 820 461 457 441 173
141 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 540 572 1075 1107 1330 1362
Size: 25 32 483 32 503 32 223 32
Cuts at: 1362 1606 1638 1885 1917 2236 2268 4003 4035
Size: 244 32 247 32 319 32 1735 32
Cuts at: 4035 4174 4206 5326 5358 5386 5418 5971 6003
Size: 139 32 1120 32 28 32 553 32
Cuts at: 6003 6508 6540 6670 6702 7282 7314 8013
Size: 505 32 130 32 580 32 699
Fragments arranged by size:
1735 1120 699 580 553 505 503 483
319 247 244 223 139 130 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 28 25
HincII GTy'rAC
Cuts at: 0 5418 5466 7197 8013
Size: 5418 48 1731 816
Fragments arranged by size:
5418 1731 816 48
KasI G'GCGC_C
Cuts at: 0 3424 3881 8013
Size: 3424 457 4132
Fragments arranged by size:
4132 3424 457
KpnI G_GTAC'C
Cuts at: 0 2193 8013
Size: 2193 5820
MluI A'CGCG_T
Cuts at: 0 2459 8013
Size: 2459 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1943 2474 3314 4949 8013
Size: 1943 531 840 1635 3064
Fragments arranged by size:
3064 1943 1635 840 531
MscI TGG'CCA
Cuts at: 0 1357 2239 3871 4597 5269 8013
Size: 1357 882 1632 726 672 2744
Fragments arranged by size:
2744 1632 1357 882 726 672
MslI CAynn'nnrTG
Cuts at: 0 338 445 899 1079 1204 2779 2887 3197
Size: 338 107 454 180 125 1575 108 310
Cuts at: 3197 3961 4222 4273 4990 5596 6026 6694 6943
Size: 764 261 51 717 606 430 668 249
Cuts at: 6943 6949 7393 7519 8013
Size: 6 444 126 494
Fragments arranged by size:
1575 764 717 668 606 494 454 444
430 338 310 261 249 180 126 125
108 107 51 6
MspA1I CmG'CkG
Cuts at: 0 104 121 653 1422 2130 2196 2262 2850
Size: 104 17 532 769 708 66 66 588
Cuts at: 2850 3207 3228 3288 4176 4500 4670 4678 4689
Size: 357 21 60 888 324 170 8 11
Cuts at: 4689 5055 5634 6198 6285 6576 7959 8013
Size: 366 579 564 87 291 1383 54
Fragments arranged by size:
1383 888 769 708 588 579 564 532
366 357 324 291 170 104 87 66
66 60 54 21 17 11 8
NaeI GCC'GGC
Cuts at: 0 615 2151 5179 6019 8013
Size: 615 1536 3028 840 1994
Fragments arranged by size:
3028 1994 1536 840 615
NarI GG'CG_CC
Cuts at: 0 3425 3882 8013
Size: 3425 457 4131
Fragments arranged by size:
4131 3425 457
NcoI C'CATG_G
Cuts at: 0 2498 2777 3356 4589 4622 6938 7401 8013
Size: 2498 279 579 1233 33 2316 463 612
Fragments arranged by size:
2498 2316 1233 612 579 463 279 33
NgoMIV G'CCGG_C
Cuts at: 0 613 2149 5177 6017 8013
Size: 613 1536 3028 840 1996
Fragments arranged by size:
3028 1996 1536 840 613
NotI GC'GGCC_GC
Cuts at: 0 3153 8013
Size: 3153 4860
NspI r_CATG'y
Cuts at: 0 286 586 1656 2217 3132 3162 6573 7613
Size: 286 300 1070 561 915 30 3411 1040
Cuts at: 7613 7695 8013
Size: 82 318
Fragments arranged by size:
3411 1070 1040 915 561 318 300 286
82 30
PasI CC'CwG_GG
Cuts at: 0 7233 8013
Size: 7233 780
PciI A'CATG_T
Cuts at: 0 1652 7691 8013
Size: 1652 6039 322
Fragments arranged by size:
6039 1652 322
PflMI CCAn_nnn'nTGG
Cuts at: 0 1187 1425 2235 2262 2376 2757 4035 4754
Size: 1187 238 810 27 114 381 1278 719
Cuts at: 4754 5280 5820 6072 8013
Size: 526 540 252 1941
Fragments arranged by size:
1941 1278 1187 810 719 540 526 381
252 238 114 27
PfoI T'CCnGG_A
Cuts at: 0 1478 1814 1889 2912 3524 3773 4883 5249
Size: 1478 336 75 1023 612 249 1110 366
Cuts at: 5249 5729 7322 7874 8013
Size: 480 1593 552 139
Fragments arranged by size:
1593 1478 1110 1023 612 552 480 366
336 249 139 75
PmlI CAC'GTG
Cuts at: 0 1187 2084 2349 2355 6139 6969 8013
Size: 1187 897 265 6 3784 830 1044
Fragments arranged by size:
3784 1187 1044 897 830 265 6
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 1870 1902 7048 7080 8013
Size: 178 32 1660 32 5146 32 933
Fragments arranged by size:
5146 1660 933 178 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 129 1597 2718 4956 5112 5658 7320 8013
Size: 129 1468 1121 2238 156 546 1662 693
Fragments arranged by size:
2238 1662 1468 1121 693 546 156 129
PshAI GACnn'nnGTC
Cuts at: 0 1852 6988 8013
Size: 1852 5136 1025
Fragments arranged by size:
5136 1852 1025
PspOMI G'GGCC_C
Cuts at: 0 2689 5384 5532 5847 6835 7752 8013
Size: 2689 2695 148 315 988 917 261
Fragments arranged by size:
2695 2689 988 917 315 261 148
PspXI vC'TCGA_Gb
Cuts at: 0 4525 8013
Size: 4525 3488
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 949 981 1627 1659 4243 4275 7690 7722
Size: 949 32 646 32 2584 32 3415 32
Cuts at: 7722 8013
Size: 291
Fragments arranged by size:
3415 2584 949 646 291 32 32 32
32
PstI C_TGCA'G
Cuts at: 0 272 281 785 2708 3227 3632 3698 4357
Size: 272 9 504 1923 519 405 66 659
Cuts at: 4357 4475 4508 4787 5009 5633 5666 5726 5972
Size: 118 33 279 222 624 33 60 246
Cuts at: 5972 6104 6176 6203 7276 8013
Size: 132 72 27 1073 737
Fragments arranged by size:
1923 1073 737 659 624 519 504 405
279 272 246 222 132 118 72 66
60 33 33 27 9
PvuII CAG'CTG
Cuts at: 0 653 1422 2130 2196 2262 3228 3288 4500
Size: 653 769 708 66 66 966 60 1212
Cuts at: 4500 4670 4678 5055 5634 6198 6285 8013
Size: 170 8 377 579 564 87 1728
Fragments arranged by size:
1728 1212 966 769 708 653 579 564
377 170 87 66 66 60 8
RsrII CG'GwC_CG
Cuts at: 0 2000 8013
Size: 2000 6013
SacI G_AGCT'C
Cuts at: 0 602 1952 7937 8013
Size: 602 1350 5985 76
Fragments arranged by size:
5985 1350 602 76
SacII CC_GC'GG
Cuts at: 0 122 8013
Size: 122 7891
SalI G'TCGA_C
Cuts at: 0 5464 7195 8013
Size: 5464 1731 818
Fragments arranged by size:
5464 1731 818
SapI GCTCTTCn'nnn_
Cuts at: 0 376 490 3238 4885 6104 6469 6730 8013
Size: 376 114 2748 1647 1219 365 261 1283
Fragments arranged by size:
2748 1647 1283 1219 376 365 261 114
SbfI CC_TGCA'GG
Cuts at: 0 6104 8013
Size: 6104 1909
ScaI AGT'ACT
Cuts at: 0 211 1054 2059 2233 5839 8013
Size: 211 843 1005 174 3606 2174
Fragments arranged by size:
3606 2174 1005 843 211 174
SexAI A'CCwGG_T
Cuts at: 0 1433 8013
Size: 1433 6580
SfcI C'TryA_G
Cuts at: 0 268 277 781 929 2184 2625 2704 3223
Size: 268 9 504 148 1255 441 79 519
Cuts at: 3223 3628 3694 4353 4471 4504 4540 4783 5005
Size: 405 66 659 118 33 36 243 222
Cuts at: 5005 5629 5662 5722 5968 6100 6172 6199 6774
Size: 624 33 60 246 132 72 27 575
Cuts at: 6774 7020 7272 8013
Size: 246 252 741
Fragments arranged by size:
1255 741 659 624 575 519 504 441
405 268 252 246 246 243 222 148
132 118 79 72 66 60 36 33
33 27 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1022 8013
Size: 1022 6991
SfoI GGC'GCC
Cuts at: 0 3426 3883 8013
Size: 3426 457 4130
Fragments arranged by size:
4130 3426 457
SmlI C'TyrA_G
Cuts at: 0 4525 5020 5974 6298 7171 7189 7600 7936
Size: 4525 495 954 324 873 18 411 336
Cuts at: 7936 8013
Size: 77
Fragments arranged by size:
4525 954 873 495 411 336 324 77
18
SphI G_CATG'C
Cuts at: 0 286 586 3132 6573 8013
Size: 286 300 2546 3441 1440
Fragments arranged by size:
3441 2546 1440 300 286
StuI AGG'CCT
Cuts at: 0 1282 2710 4570 4900 6094 8013
Size: 1282 1428 1860 330 1194 1919
Fragments arranged by size:
1919 1860 1428 1282 1194 330
StyI C'CwwG_G
Cuts at: 0 989 1265 2498 2777 3356 3431 4388 4589
Size: 989 276 1233 279 579 75 957 201
Cuts at: 4589 4598 4622 4952 5819 6938 7401 7977 8013
Size: 9 24 330 867 1119 463 576 36
Fragments arranged by size:
1233 1119 989 957 867 579 576 463
330 279 276 201 75 36 24 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1852 1957 2017 3369 3491 5185 6844 7756
Size: 1852 105 60 1352 122 1694 1659 912
Cuts at: 7756 8013
Size: 257
Fragments arranged by size:
1852 1694 1659 1352 912 257 122 105
60
TatI w'GTAC_w
Cuts at: 0 209 767 1052 2057 2231 5837 7706 8013
Size: 209 558 285 1005 174 3606 1869 307
Fragments arranged by size:
3606 1869 1005 558 307 285 209 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 704 736 2755 2787 4543 4575 4966 4998
Size: 704 32 2019 32 1756 32 391 32
Cuts at: 4998 5725 5757 6833 6865 8013
Size: 727 32 1076 32 1148
Fragments arranged by size:
2019 1756 1148 1076 727 704 391 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 37 5400 7294 7656 8013
Size: 37 5363 1894 362 357
Fragments arranged by size:
5363 1894 362 357 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1350 1839 2505 2754 3069 3108 4959 5277
Size: 1350 489 666 249 315 39 1851 318
Cuts at: 5277 5281 5406 5484 5703 5760 5997 6696 6822
Size: 4 125 78 219 57 237 699 126
Cuts at: 6822 7899 8013
Size: 1077 114
Fragments arranged by size:
1851 1350 1077 699 666 489 318 315
249 237 219 126 125 114 78 57
39 4
XhoI C'TCGA_G
Cuts at: 0 4525 7189 8013
Size: 4525 2664 824
Fragments arranged by size:
4525 2664 824
XmnI GAAnn'nnTTC
Cuts at: 0 2921 5245 5311 8013
Size: 2921 2324 66 2702
Fragments arranged by size:
2921 2702 2324 66
ZraI GAC'GTC
Cuts at: 0 6081 8013
Size: 6081 1932
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AclI AgeI AscI AseI AsiSI BaeI BciVI BfrBI
BmtI BsrGI BstBI BstEII BstZ17I ClaI DraI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PmeI PsiI PvuI SanDI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XbaI XmaI