################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoT22I FbaI FseI FspI HindIII KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI NdeI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfiI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AclI 667 AACGTT AA^CGTT AcsI 1238 AAATTC [GA]^AATT[CT] AcyI 229 GGCGCC G[GA]^CG[CT]C Aor51HI 351 AGCGCT AGC^GCT ApaI 1030 GGGCCC GGGCC^C ApoI 1238 AAATTC [GA]^AATT[CT] Asp700I 1184 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 793 GAGCTC G[AT]GC[AT]^C BanI 229 GGCGCC G^G[CT][GA]CC BbeI 229 GGCGCC GGCGC^C BbsI 1378 GAAGAC GAAGAC(2/6) BpuAI 1378 GAAGAC GAAGAC(2/6) BsaHI 229 GGCGCC G[GA]^CG[CT]C BsiHKAI 793 GAGCTC G[AT]GC[AT]^C BsmBI 542 CGTCTC CGTCTC(1/5) BsmFI 357 GGGAC GGGAC(10/14) BsrBI 20 CCGCTC CCGCTC(-3/-3) BstBI 911 TTCGAA TT^CGAA BstEII 39 GGTGACC G^GT[ACGT]ACC BstPI 39 GGTGACC G^GT[ACGT]ACC BstYI 176 AGATCC [GA]^GATC[CT] DraI 447 TTTAAA TTT^AAA DraII 1029 AGGGCCC [GA]G^G[ACGT]CC[CT] DsaI 95 CCATGG C^C[GA][CT]GG Eco47III 351 AGCGCT AGC^GCT EcoNI 1311 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1029 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 39 GGTGACC G^GT[ACGT]ACC EcoRV 658 GATATC GAT^ATC HgaI 359 GACGC GACGC(5/10) Hin1I 229 GGCGCC G[GA]^CG[CT]C HincII 858 GTTAAC GT[CT]^[GA]AC HindII 858 GTTAAC GT[CT]^[GA]AC HpaI 858 GTTAAC GTT^AAC KasI 229 GGCGCC G^GCGCC MflI 176 AGATCC [GA]^GATC[CT] NarI 229 GGCGCC GG^CGCC NcoI 95 CCATGG C^CATGG NheI 114 GCTAGC G^CTAGC NspI 752 GCATGC [GA]CATG^[CT] NspV 911 TTCGAA TT^CGAA PflMI 717 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 988 GACTC GAGTC(4/5) Psp1406I 667 AACGTT AA^CGTT SacI 793 GAGCTC GAGCT^C SapI 470 GAAGAGC GCTCTTC(1/4) SexAI 35 ACCTGGT A^CC[AT]GGT SfoI 229 GGCGCC GGC^GCC SfuI 911 TTCGAA TT^CGAA SmaI 159 CCCGGG CCC^GGG SphI 752 GCATGC GCATG^C StuI 771 AGGCCT AGG^CCT Van91I 717 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 176 AGATCC [GA]^GATC[CT] XmaCI 159 CCCGGG C^CCGGG XmaI 159 CCCGGG C^CCGGG XmnI 1184 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 177 GATCC GGATC(4/5) 828 GATCC AlwNI 31 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 574 CAGCTCCTG AvaI 159 CCCGGG C^[CT]CG[GA]G 1312 CTCGGG BanII 793 GAGCTC G[GA]GC[CT]^C 1030 GGGCCC BsgI 476 CTGCAC GTGCAG(16/14) 837 CTGCAC BsmAI 1060 GAGAC GTCTC(1/5) 543 GTCTC BsoBI 159 CCCGGG C^[CT]CG[GA]G 1312 CTCGGG BsrFI 241 GCCGGC [GA]^CCGG[CT] 283 GCCGGC BstNI 36 CCTGG CC^[AT]GG 843 CCTGG BstXI 665 CCAACGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1130 CCAAAGACTTGG Cfr10I 241 GCCGGC [GA]^CCGG[CT] 283 GCCGGC EcoRII 36 CCTGG ^CC[AT]GG 843 CCTGG FauI 19 CCCGC CCCGC(4/6) 1123 CCCGC HaeII 229 GGCGCC [GA]GCGC^[CT] 351 AGCGCT MvaI 36 CCTGG CC^[AT]GG 843 CCTGG NaeI 241 GCCGGC GCC^GGC 283 GCCGGC NgoMIV 241 GCCGGC G^CCGGC 283 GCCGGC TaqI 912 TCGA T^CGA 1343 TCGA TthHB8I 912 TCGA T^CGA 1343 TCGA XcmI 585 CCAGTCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1095 CCAAGACCACAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstUI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvnI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI